Persistent Changes of Peripheral Blood Lymphocyte Subsets in Patients with Oral Squamous Cell Carcinoma (original) (raw)
Related papers
European Journal of Cancer. Part B: Oral Oncology, 1996
Frequencies of proliferating and cytotoxic lymphocytes from the peripheral blood and tumour tissue of oral cancer patients and healthy individuals were monitored using limiting dilution analysis. Significantly lower precursor frequencies of proliferating lymphocytes were observed in the peripheral blood and tumour tissue of oral cancer patients. A high frequency of natural killer (NK) cells but low cytotoxic T lymphocytes (CTL) was observed in the peripheral blood compartment of oral cancer patients as compared to healthy individuals. A marked reduction in both NK and CTL frequencies in the tumour tissue compared to the peripheral blood was observed. In the tumour tissues, increased percentages of activated CD4 + lymphocytes as compared to CD8 + lymphocytes were observed. Our results suggest that impaired proliferative and cytotoxic potential of tumour infiltrating lymphocytes may play an important role in the escape of tumour cells from the immune system.
Analytical Cellular Pathology
Head and neck squamous cell carcinoma (HNSCC) is a common type of cancer worldwide. Strong connections have been revealed between immune cells and the pathogenesis of HNSCC. Important differences regarding the levels of immune cell subpopulations in both peripheral circulation and tumor microenvironment were emphasized, with some of them having prognostic significance. In our study, we performed an analysis of immune changes in the tumor tissue and the peripheral blood of untreated HNSCC patients, investigating the proportions of different immune cell populations in these two compartments. The local infiltrating lymphocytes were mainly cytotoxic T cells (CD8+). We have also revealed an increased level of B lymphocytes (CD19+) in the tumor microenvironment. In peripheral blood, the most important lymphocyte subtype was represented by the helper T lymphocytes (CD4+). We also found an increased proportion of circulating NK cells (CD56+). Our results showed significant differences betwe...
International journal of scientific research, 2018
Background & Objectives: In OSCC, the presence of regional lymph node metastasis at presentation is the most significant adverse prognostic factor and a major determinant of poor survival. Tumor-infiltrating lymphocytes (TILs) often infiltrate solid malignant tumours and extensive lymphocyte infiltration has been related with a more favourable prognosis in patients with various cancers. OSCC often contain large mononuclear cell infiltrates, comprised mainly of T cells, which could reflect an in situ immune reaction against the malignant OSCC cells. The aim of this retrospective study was to evaluate the expression of Cytotoxic T lymphocyte in OSCC using immunohistochemical marker CD8+(CTLs) and correlate these findings with the status of lymphnode. Methods: The study was conducted on tissue sections obtained from histopathologically diagnosed cases of OSCC (n=30) retrieved from the archives of Department of Oral and Maxillofacial Pathology. The sample consisted of cases showing lymph node metastasis (n=15) and those without pathologic lymph node involvement (n=15). The sections were evaluated by using immunohistochemical staining technique with marker CD8 for Cytotoxic T lymphocytes. The mean immunoexpression of Cytotoxic T lymphocyte was evaluated and correlated with lymphnode status. Results: A statistically significant increase in the count of CTLs (CD8+) was observed in lymph node negative pN(-) as compared to lymph node positive cases pN(+) of OSCC. Interpretation & Conclusion: CTLs (CD8+) are involved in modulating the immune response and can contribute to the dissemination or control of metastatic neoplastic cells. It can be considered that T-cell mediated adaptive immunity plays a key role in anti-tumour immunity.
PLoS ONE, 2012
Head and neck squamous cell carcinoma (HNSCC) is known to cause substantial immunosuppression. The present study was designed to characterize blood leukocyte activation in HNSCC and to investigate if the individual activation pattern could be related to tumor progress and survival. The leukocyte activation profile of HNSCC patients and healthy controls was assessed with flow cytometry. HNSCC patients displayed increased numbers of monocytes, neutrophils and total leukocytes as well as an enhanced neutrophil/lymphocyte ratio. In addition, patients had a higher percentage of CD69 + , CD71 + and CD98 + T cell subsets and NK cells, and a reduced expression of L-selectin in CD14 high CD16 + monocytes and neutrophils, when compared to controls. These changes could be correlated to both tumor burden and spread to lymph nodes. Among the cancer patients an increased neutrophil/lymphocyte ratio, a low neutrophil and CD14 high CD16 + monocyte activation state and an elevated CD4/CD8 ratio were related to poor survival. In contrast, a high percentage of CD98 + Th cells appeared to be associated with a better outcome. Taken together, the present data indicate that HNSCC causes activation of blood leukocytes and that the individual activation pattern can be linked to prognosis.
2010
Oral squamous cell carcinoma (OSCC) is common in many Asian countries. The immunopathogenesis of OSCC is unclear. The authors analyzed the lymphocyte subtypes and surface activation markers in healthy Taiwanese people (n = 130) and patients with OSCC (n = 97)/oral leukoplakia (OL, n = 28) using flow cytometry. Univariate analysis found an elevation in the percentage of CD56+ NK cells, CD4+/CD69+ T cells, CD19+/CD69+ B cells and CD56+/CD69+ NK cells in OSCC patients relative to healthy people. The CD19+ and CD19+/CD25+ lymphocyte subtypes decreased in OSCC patients. CD56+ NK cells increased in OL patients. CD56+/CD69+ NK cells were elevated in recurrent and advanced OSCC. Multivariate analysis revealed an increase in CD56+ NK and CD19+/CD69+ cells in OL patients relative to controls. CD19+ B cells declined during progression from OL to OSCC. Betel quid chewing, alcohol, smoking, tumour location and staging showed little effect on lymphocyte subtypes. These results suggest that alterations and activation of NK cells, T and B cells are important and associated with disease status in oral carcinogenesis.
T-lymphocyte subpopulations in peripheral blood and tissues of cancer patients
Cancer research, 1980
Patients with cancer often show impaired immune functions; however, the basis of this suppression is still not understood. In several experimental systems, human T-cells with receptors for Fc of immunoglobulin G may function as suppressors, and those with receptors for Fc of immunoglobulin M may function as helpers. Peripheral blood as well as tumor tissue infiltrates were examined for proportions and numbers of T gamma, T mu, or Ia-positive T-cells. Forty-five untreated patients with solid tumors and 24 patients with lymphomas were studied. An increase in the percentage of peripheral blood T gamma cells (p < 0.001) and a decrease in T mu cells (p < 0.0005) were recorded in all tumor patients when compared with 30 normal controls. Percentages and absolute numbers of peripheral blood Ia-positive T-cells were decreased (p < 0.001 and < 0.00001) in solid-tumor patients; by contrast, the proportion of peripheral blood Ia-positive T-cells was elevated (p < 0.005) in lympho...
BMC Immunology, 2022
Background: A crucial role for the immune system has been proposed in the establishment and progression of head and neck squamous cell carcinoma (HNSCC). In this study, we investigated the cytokine and regulatory profiles of T cells in tumor draining lymph nodes (TDLNs) of patients with HNSCC. Results: The frequencies of CD4 + TNF-α + and CD4 + TNF-α hi negatively were associated with poor prognostic factors such as LN involvement (P = 0.015 and P = 0.019, respectively), stage of the disease (P = 0.032 and P = 0.010, respectively) and tumor size (P = 0.026 and P = 0.032, respectively). Frequencies of CD8 + IFN-γ + and CD8 + IFN-γ + TNF-α + T cells showed negative relationship with tumor grade (P = 0.035 and P = 0.043, respectively). While, the frequencies of CD4 + IL-4 + , CD8 + IL-10 + , CD8 + IL-4 + T cells were higher in advanced stages of the disease (P = 0.042, P = 0.041 and P = 0.030, respectively) and CD4 + IFN-γ + TNF-α − , CD8 + IL-4 + and CD8 + IFN-γ + TNF-α − T cells were higher in patients with larger tumor size (P = 0.026 and P = 0.032, respectively). Negative associations were found between the frequencies of CD4 + CD25 + Foxp3 + and CD4 + CD25 + Foxp3 + CD127 low/− Treg cells and cancer stage (P = 0.015 and P = 0.059). Conclusion: This study shed more lights on the changes in immune profile of T cells in TDLNs of HNSCC. Larger tumor size and/or LN involvement were associated with lower frequencies of CD4 + TNF-α + , CD8 + IFN-γ + and CD8 + IFN-γ + TNF-α + but higher frequency of CD4 + IL-4 + T cells. Moreover, Foxp3 + Tregs correlated with good prognostic indicators.
T-Lymphocyte Subpopulations in Peripheral Blood and Tissues of Cancer Patients1
Cancer Research
Patients with cancer often show impaired immune functions; however, the basis of this suppression is still not understood. In several experimental systems, human T-cells with receptors for Fc of immunoglobulin G may function as suppressors, and those with receptors for Fc of immunoglobulin M may function as helpers. Peripheral blood as well as tumor tissue infiltrates were examined for proportions and numbers of Ty, T/i, or lapositive T-cells. Forty-five untreated patients with solid tumors and 24 patients with lymphomas were studied. An increase in the percentage of peripheral blood Ty cells (p < 0.001) and a decrease in T/i cells (p < 0.0005) were recorded in all tumor patients when compared with 30 normal controls. Percentages and absolute numbers of peripheral blood la-positive T-cells were decreased (p < 0.001 and < 0.00001) in solid-tumor patients; by contrast, the proportion of peripheral blood lapositive T-cells was elevated (p < 0.005) in lymphoma sub jects. Studies of cancer tissues from 46 untreated patients using immunofluorescence and mouse hybridoma antibody specific for T-cells showed that tumor lymphocytic infiltrates were composed mainly of T-cells. Double staining with fluorescein-conjugated specific anti-Ty and anti-human la reagents detected relatively high proportions of Ty and la-positive Tcells within solid-tumor lymphoid infiltrates. A comparison of peripheral blood and tumor lymphocyte T-cell profiles revealed that, in some patients, low proportions of la-positive T-cells in blood were paralleled by a high percentage of such cells in tumor lymphoid infiltrates.