An audit of specimens received for superficial fungal studies to the Department of Microbiology, University of Ruhuna (original) (raw)
Related papers
Clinico-Mycological Study of Dermatophytic Infections in a Tertiary Care Hospital of South Delhi
Journal of Evidence Based Medicine and Healthcare, 2020
BACKGROUND Dermatophytosis is a superficial fungal infection commonly referred to as ringworm or tinea. It remains a public health problem, which is prevalent in all age groups and both the sexes. The prevalence and mycological profile of dermatophytosis varies across populations and also keeps changing from time to time in a particular population. This study was conducted to study the clinicomycological profile of dermatophytic infection in our population. METHODS 117 patients over a period of one year were enrolled for the study. All patients were thoroughly examined, and clinical details were noted. The sample obtained by skin / nail / hair scrapings was divided into two parts: one for KOH mount and another for fungal culture. Following direct microscopic examination with KOH, the scrapings (skin, hair and nail) were inoculated into slopes of duplicate sets of tubes containing Sabouraud's dextrose agar and dermatophyte test medium. The isolates were identified by studying the culture characteristics, pigment production, and microscopic examination of the lactophenol cotton blue mounts and slide culture. RESULTS Males were found to be more commonly infected (M : F = 2.4 : 1). The most common age group affected was 16-30 years (49 patients, 41.8 %) followed by 31-45 years age group (35 patients, 29.9 %). Tinea corporis was the most common clinical presentation (54, 46.1 %) followed by tinea manuum (21, 17.9 %), tinea cruris (13, 11.1 %), tinea unguium (13, 11.1 %), tinea faciei (6, 5.1 %), tinea pedis (5, 4.2 %) and mixed (5, 4.2 %). In 52 (44.4 %) patients, KOH positivity was seen while 48 (41 %) patients showed culture positivity. The most common dermatophytes isolated were T. mentagrophytes (25, 59.5 %) followed by T. rubrum (10, 23.8 %), T. tonsurans (6, 14.2 %) and Epidermophyton floccosum (1, 2.3 %). CONCLUSIONS T. mentagrophytes is the most common organism causing dermatophytosis in our population. An epidemiological shift of type of fungus seems to have occurred when compared to previous study in which T. rubrum was the most common organism isolated. Also, most common clinical pattern observed was tinea corporis followed up by tinea manuum which is different from the previously observed pattern. The present study demonstrated the clinico-mycological pattern in our population which can be utilized in framing policies and treatment guidelines.
2018
Tinea capitis is one of the commonest forms of superficial mycosis that we come across in the dermatology OPD, though the etiology of the disease is not regularly documented. Here among 72 clinically diagnosed cases of tinea capitis, the age group mostly affected was found to be between 0-10 years (58.3%). After direct microscopy it was found that 60 (83.3%) were positively showing presence of filamentous fungi under KOH mount in direct microscopy. The number of samples showing ectothrix type of infection was higher (70%) than the samples showing endothrix type of infection (23.3%). Among the 72 cases, 48 (66.6%) cases were culture positive for dermatophytes. The rest were either showing no growth after 45 days of incubation (27.8%), or found to have growth of other non dermatophytic fungi like Aspergillus spp. (4.2%) or Fusarium spp. (1.6%). The Male: Female ratio among culture positive cases was 1.25:1. Though the culture of dermatophytes showed predominance of Trichophyton (66.7%...
Clinicomycological study of tinea infections in and around Pune
International Journal of Research in Dermatology, 2019
Background: Tinea is a common fungal infection seen in the tropical and subtropical countries affecting the skin and its appendages. The presentation may vary from mild scaling to severe inflammation with bacterial super infection. It may be confused with other manifestation such as psoriasis, seborrhea, drug eruptions, eczema, and contact dermatitis. Hence correct diagnosis is necessary for appropriate treatment, which will reduce morbidity, discomfort and lessens possibility of transmissions. The aims and objectives were to determine clinicomycological profile of Tinea infections in patients attending dermatology OPD of B. J. Govt. Medical College and Sassoon General Hospital, Pune. Methods: Skin scrapings, nail clippings; hair samples from clinically suspected cases of tinea were collected. Identification of dermatophytes from these samples was done by conventional technique. Results: 119 clinically suspected cases of Tinea infections were processed over a period of one year. Out of these cases mixed infection of Tinea cruris with corporis was the predominant (27.73%) clinical presentation. Among all the samples, fungal filaments were seen by KOH mount in 48 (40.33%) whereas 35 (29.41%) samples were confirmed as dermatophytes by culture. Among these 35 isolates of dermatophytes 20 were T. rubrum, 7 isolates were T. tonsurans. 8 isolates were of T. mentagrophytes. Conclusions: In present study mixed infections of tinea cruris with corporis was the predominant clinical presentation and T. rubrum was the most common dermatophyte isolated.
A clinicomycological study of dermatophytic infection in a tertiary care hospital
IP Innovative Publication Pvt. Ltd., 2018
Introduction: Dermatophytosis is a mycotic infection of the hair, skin or nails. Although dermatophytic infection doesn’t cause mortality, it can lead to morbidity and result in a major public health issue usually in tropical countries like India, because of the supporting climatic conditions like high temperature and air moisture. The present study was undertaken to know the clinical status of dermatophytic infection and to isolate and identify the species commonly seen in our area. Materials and Methods: A total of 166 clinically suspected cases of dermatophytosis sent for department of Microbiology were studied over a period of one year. KOH for microscopy and culture on Sabouraud's dextrose agar slopes was done. Species identification was done by studying growth morphology, slide culture and biochemical tests like urease test. Results: Among 166 patients, the dermatophytosis was more common among the age group of 21-30 years. Males (55.5%) were more affected than females (44.5%). The most common clinical type was tinea corporis (36.2%) followed by tinea incognito (10.3%). Out of 166 patients, 125 cases (75.3%) were positive for fungi by KOH and 118 cases (71.1%) showed growth in culture. Trichophyton spp are predominantly isolated (88%). Conclusion: The prevalence of dermatophytosis usually differ with respect to various geographical locations. India due to high humidity and temperature acts as a rich ground for the heavy growth of dermatophytes. Idea regarding the fungal infections prevalent in the particular region is important to know the burden of infection and its course of infection. This helps to plan for the proper infrastructure required for various epidemiological and laboratory studies, and further to evaluate interventions required for treatment. Keywords: Dermatophytosis, Trichophyton, Tinea.
Microbiology Research Journal International, 2019
Dermatophytosis or tinea is a type of cutaneous infection caused by keratinophilic fungi, infecting the skin, nails and hair. A correct diagnosis is important for epidemiological purposes and initiating appropriate treatment. An increase in the prevalence of fungal infection worldwide is due to abuse of antibiotics, immunosuppressive treatments and numerous medical conditions. Aim: To isolate, identify, and examine the in-vitro antifungal susceptibility of dermatophytes in clinically suspected cases of tinea infections. Methodology: After taking informed consent, we took 65 patients suspected of tinea infection and obtained samples from skin, hair and nail, under aseptic precautions, at Department of Microbiology, University of Health Sciences (UHS), Lahore, Pakistan. The identification of dermatophytes was performed using potassium hydroxide (KOH) mounts and culture on Sabouraud Dextrose Agar (SDA) and Dermatophyte Test Medium (DTM). The cultures were incubated at 30ºC for up to 4 ...
Mycological Study of Tinea Capitis in a Tertiary Care Hospital
Journal of Evolution of medical and Dental Sciences, 2015
BACKGROUND AND OBJECTIVE Tinea capitis is a superficial fungal infection of the hair follicle of scalp. The causative dermatophytes vary with social status and geographical distribution. The incidence is high in developing countries due to factors like poverty compounded by overcrowding, improper hygiene and illiteracy. Tinea capitis is the most common dermatophytes infection of childhood, especially in school going children. It is self-limiting disease of childhood and seldom extends beyond puberty. India being a tropical developing country with high temperature and humidity favours superficial cutaneous mycotic infections. The objective of this study was to isolate and identify the dermatophytes causing tinea capitis. METHOD A prospective study was conducted from July 2013 to August 2015. The study included 90 clinically suspected cases of tinea capitis. Among the 90 clinically suspected cases, 51 cases showed mycological evidence of fungal infection. The confirmed positive cases were then speciated based on their cultural morphology and microscopy. RESULTS Among the 51 culture positive cases-20(39.21%) Trichophyton rubrum, 14(27.45%) Trichophyton mentagrophytes, 06(11.76%) Trichophyton tonsurans, 05(9.8%) Microsporum gypseum, 03(5.8%) Microsporum canis, 02(3.92%) Microsporum audouinii and 01(1.96%) Trichophyton violaceum species were obtained. CONCLUSION Trichophyton species were found to be the major causative agents for tinea capitis
Asian Journal of Pharmaceutical and Clinical Research Journal, 2022
Methods: An observational prospective study was carried out at large tertiary care hospital in Southern Maharashtra, India. 110 Participants were selected based on Inclusion and exclusion criteria. Data collection was done with help of personal interview and detailed examination by investigator using predesigned, pre-tested, and structured questionnaire. All patients were followed up in dermatology department till complete investigation, treatment, and discharge. Results: Patients belonging to 21−40 year constituted 45% of the study population. Male to female ratio was 3:1. About 51.82% belonged to low socioeconomic status and 56.36% were from rural areas. The most common isolate obtained was Trichophyton rubrum (25.45%) followed by Trichophyton mentagrophytes (7.27%). Out of the 110 samples collected, 66.36% (73 samples) were KOH positive and 35.45% (39 samples) were culture positive. The most common type of mixed dermatophytic infection was Tinea Corporis with Tinea Cruris (38.46%) followed by Tinea Manuum with Tinea Unguium (30.77%). Mixed type was seen more commonly in 21−40 years age group (30.77%). Association of isolate and the clinical type involved among study participants was assessed by applying Chi-square test which showed no statistical significance (p=0.94). Similarly, association of results of KOH mount and culture report to clinical types also showed no statistical significance (p=0.94). However, when association of age and sex with clinical types was assessed, age showed statistically significant association (p=0.004) while sex showed no statistical significance (p=0.32). Conclusions: Incidence of dermatophytosis was maximum in rural areas, low socioeconomic group and in summer. Thus, changing environmental and socioeconomic conditions often led to changing epidemiology of dermatophytic infections. Tinea corporis was found to be the commonest clinical type followed by Tinea cruris. T. rubrum was the commonest isolate obtained (25.45%). Fungi were demonstrated by direct microscopy and/or by culture in 73 cases (66.36%) out of 110 cases. Hence, direct microscopy with or without culture is an important diagnostic tool in dermatophytosis. Authors recommend more in-depth study with larger sample size and multicentric based to have clearer picture of dermatophytosis.
A preliminary clinico-mycological study of dermatophytes infection
International Journal of Research in Medical Sciences
Background: Superficial fungal infections are the most common skin diseases, affecting millions of people throughout the world. Aim of the study was carried out to find, the effect of seasonal variation and socio-economic status on the prevalence of dermatophytes.Methods: A total of fifty clinically suspected cases of dermatophytosis were subjected to mycological studies.Results: Out of which 32 (64%) were culture positive Tinea corporis was the most common clinical type followed by Tinea cruris. Tricophoton rubrum 17/32 (53.12%) was the most common isolate followed by T. mentagrophytes 9/32(28.12%), T. tonsurans 3/32(9.37%), T. verrrucosum 2/32(6.25%) and T. schoenleinii 1/32(3.12%). Most of the patients belonged to the middle socio-economic group (28/50) followed by lower socio-economic group (19/50) maximum cases of dermatophytosis were reported from June to August revealing the fact that hot and humid weather during the monsoons has a great impact on the occurrence of dermatophy...
A Clinical-Mycological and Immunological Study of a Wide Spread Tinea Corporis
A wide spread Tinea corporis infections might be a tinea incognito which is a dermatophyte infection with atypical clinical features modified by the improper use of corticosteroids or calcineurin inhibitors, or due to poverty, poor hygiene, and unsanitary conditions. A total of 100 patients was investigated, 60 patients were 60 females and 40 males, female to male ratio 1.5 were included in the study. Tinea corporis was most prevalent in thethirties. The size of the individual skin lesion was more than 5cm up to 50cm. The mean duration of the disease was 9.5months (range 6-12 months). Sixty patients had a history of treatment with topical steroids because of missing the diagnosis as eczema and psoriasis. Microscopical examination revealed hyphae spores in most of the cases n=84 (84%). Mycological culture were positive in 93 cases (93%). The most frequently isolated dermatophyte had been Trichophyton rubrum, n= 53 cases (56% out of 93). This case series revealed Trichophyton rubrum as the most frequent agent of a wide spread tinea corporis. Immunological assay revealed no changes in the serum level of IgM and IgA, in IgG and C3 serum levels increase in 40 cases, normal in 50 cases, and decrease in 10 cases, whereas C4 serum level increase in 20 cases, normal in 40 cases, and decrease in 40 cases.