Engineering Protein Dynamics of Ancestral Luciferase (original) (raw)

Engineering the protein dynamics of an ancestral luciferase

Joan Planas

Nature Communications

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Directed Molecular Evolution Reveals Gaussia Luciferase Variants with Enhanced Light Output Stability

Sarah Bovenberg, Hannah Degeling

Analytical Chemistry, 2013

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Super RLuc8: A novel engineered Renilla luciferase with a red-shifted spectrum and stable light emission

mahboobeh nazari

Enzyme and Microbial Technology, 2017

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Bioluminescent and structural features of native folded Gaussia luciferase

Eugene Vysotski

Journal of Photochemistry and Photobiology B: Biology, 2018

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Effect of mutation at positively charged residues (K329 and R330) in a flexible region of firefly luciferase on structure and kinetic properties

Saman Hosseinkhani

Enzyme and microbial technology, 2019

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Engineering the C-terminus of firefly luciferase as an indicator of covalent modification of proteins

Stephanie Matthews

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1996

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Optimization of the Turnover in Artificial Enzymes via Directed Evolution Results in the Coupling of Protein Dynamics to Chemistry

ioanna zoi

Journal of the American Chemical Society, 2019

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Coelenterazine-v ligated to Ca2+-triggered coelenterazine-binding protein is a stable and efficient substrate of the red-shifted mutant of Renilla muelleri luciferase

Natalia Malikova

Analytical and Bioanalytical Chemistry, 2010

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The role of proline substitutions within flexible regions on thermostability of luciferase

Yiru Gan

Biochimica et biophysica acta, 2015

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A catalytic mechanism for Renilla-type bioluminescence

Floros Flouros

2022

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Exploring the challenges of computational enzyme design by rebuilding the active site of a dehalogenase

Veselin Kolev

Proceedings of the National Academy of Sciences, 2018

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Rescue of Conformational Dynamics in Enzyme Catalysis by Directed Evolution

Dorothee Kern

2017

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Design of thermostable luciferases through arginine saturation in solvent-exposed loops

Saman Hosseinkhani

Protein Engineering, Design and Selection, 2011

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Functional consequences of site-directed mutation of conserved histidyl residues of the bacterial luciferase .alpha. subunit

Xin Xing

Biochemistry Usa, 1991

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Evolving strategies for enzyme engineering

Peter Meinhold

Current Opinion in Structural Biology, 2005

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Incorporating Fast Protein Dynamics into Enzyme Design: A Proposed Mutant Aromatic Amine Dehydrogenase

ioanna zoi

The journal of physical chemistry. B, 2017

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The Influence of Insertion of a Critical Residue (Arg356) in Structure and Bioluminescence Spectra of Firefly Luciferase

Saman Hosseinkhani

Journal of Biological Chemistry, 2007

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Pathways and Mechanisms for Product Release in the Engineered Haloalkane Dehalogenases Explored Using Classical and Random Acceleration Molecular Dynamics Simulations

Jíří Damborský

Journal of Molecular Biology, 2009

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Dynamics and hydration explain failed functional transformation in dehalogenase design

Jíří Damborský

Nature Chemical Biology, 2014

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Probing the emitter site of Renilla luciferase using small organic molecules; an attempt to understand the molecular architecture of the emitter site

mahboobeh nazari

International Journal of Biological Macromolecules, 2016

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Novel methods for directed evolution of enzymes: quality, not quantity This review comes from a themed issue on Protein technologies and commercial enzymes Edited

Lorenzo Camisi

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How directed evolution reshapes the energy landscape in an enzyme to boost catalysis

vy nguyen

Science, 2020

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De novo design of luciferases using deep learning

Pengchen Ma

Nature

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Accelerated protein engineering for chemical biotechnology via homologous recombination

Lorenzo Camisi

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Biotechnological Advances in Luciferase Enzymes

Gary Sayler

Bioluminescence [Working Title]

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Directed evolution of enzymes for biocatalysis and the life sciences

Gavin P Williams

Cellular and Molecular Life Sciences, 2004

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Modulating Enzyme Catalysis through Mutations Designed to Alter Rapid Protein Dynamics

ioanna zoi

Journal of the American Chemical Society, 2016

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Implication of a critical residue (Glu175) in structure and function of bacterial luciferase

Saman Hosseinkhani

FEBS Letters, 2005

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Are Time-Dependent Fluorescence Shifts at the Tunnel Mouth of Haloalkane Dehalogenase Enzymes Dependent on the Choice of the Chromophore?

Mariana Amaro

2013

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Molecular basis of thermostability enhancement of Renilla luciferase at higher temperatures by insertion of a disulfide bridge into the structure

Zahra Kahrani

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2017

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Protein dynamics in a family of laboratory evolved thermophilic enzymes

William Goddard

2003

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Illuminating the mechanism and allosteric behavior of NanoLuc luciferase

Sérgio Marques

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