Adhesion Properties of Lactobacillus plantarum Dad-13 and Lactobacillus plantarum Mut-7 on Sprague Dawley Rat Intestine (original) (raw)
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Journal of Global Pharma Technology, 2018
The uses of microbes as functional food maintain health and prevent many disease and disorder. The isolation and research of new strains of probiotic strains especially lactobacilli proven to be useful to satisfy the increasing demand of the population. In the current study, the probiotic potential of Lactobacillus strains isolated from sheep milk was investigated. The Lactobacillus strains were identified and evaluated for tolerance against gastric acidity and bile toxicity, along with the adhesion to HT-29 cells. The study proved the antimicrobial activities and antibiotic susceptibility. Survival of the strains through the host intestine was examined by the 12 week Wister rat feeding and faecal analysis trial. The in-vivo trials not only proved the adhesion but also the survival of the Lactobacillus plantarum MCC 3595 inside the intestinal lumen of the host. Thus, the isolated strain can act as the functional food by the further clinical investigation.
Study on the Adhesion of Lactobacillus Plantarum Strains with Probiotic Properties to MDCK
2016
One of the requirements for probiotic strains is to adhere to epithelial cells or cell lines. The presence of S-layer proteins in three Lactobacillus plantarum strains with probiotic properties was examined as well as their ability to adhere to the epithelial monolayer model non-cancerous cell line MDCK. The three strains lacked S-layer proteins, but Lactobacillus plantarum X2 and Lactobacillus plantarum LBRZ12 adhered to the cells of MDCK, while Lactobacillus plantarum F3 didn’t. Along with their other probiotic properties these make them suitable for inclusion in the composition of probiotics and probiotic foods.
Adhesion Ability of Lactobacillus Plantarum AC131
Biotechnology & Biotechnological Equipment, 2011
Adhesion of lactic acid bacteria (LAB) to mucosal surfaces and their ability to prevent pathogenic invasion of the epithelium are among the most beneficial properties of probiotic lactobacilli. The molecular mechanisms of lactobacillus adhesion have not been fully studied and understood yet. Therefore, a current task is to reveal the genetic and biological factors that determine the adhesion of probiotic LAB cultures. A dairy product originating strain AC131 was selected for the present study, based on its broad-spectrum antimicrobial activity. It was identified as Lactobacillus plantarum by 16S rDNA sequence analysis. The presence of genes Map, Mub and EF-Tu, as genetic determinants of the adhesion factors in Lactobacillus plantarum AC131, was established by PCR analyses. In addition, in vitro tests for adhesive ability of the strain AC131 were carried out. Positive results in tests with two different cell lines were obtained and the strain AC131 was classified as strongly adhesive to HeLa and moderate adhesive to HT-29 cell line. Our results provide a promising basis for further characterization of Lactobacillus plantarum AC131, as a potential probiotic.
Adhesion Ability ofLactobacillus PlantarumAC131
Biotechnology & Biotechnological Equipment, 2011
Adhesion of lactic acid bacteria (LAB) to mucosal surfaces and their ability to prevent pathogenic invasion of the epithelium are among the most beneficial properties of probiotic lactobacilli. The molecular mechanisms of lactobacillus adhesion have not been fully studied and understood yet. Therefore, a current task is to reveal the genetic and biological factors that determine the adhesion of probiotic LAB cultures. A dairy product originating strain AC131 was selected for the present study, based on its broad-spectrum antimicrobial activity. It was identified as Lactobacillus plantarum by 16S rDNA sequence analysis. The presence of genes Map, Mub and EF-Tu, as genetic determinants of the adhesion factors in Lactobacillus plantarum AC131, was established by PCR analyses. In addition, in vitro tests for adhesive ability of the strain AC131 were carried out. Positive results in tests with two different cell lines were obtained and the strain AC131 was classified as strongly adhesive to HeLa and moderate adhesive to HT-29 cell line. Our results provide a promising basis for further characterization of Lactobacillus plantarum AC131, as a potential probiotic.
Evaluation of adhesion properties of lactobacilli probiotic candidates
Monatshefte für Chemie - Chemical Monthly
Bacterial adhesion is a complex phenomenon implicated in the host-bacterial interaction that is pivotal for probiotic activity. Eight probiotic lactobacilli candidates (Lactobacillus reuteri, L. plantarum, L. mucosae, L. murinus) were screened for their ability to adhere to abiotic and biotic surfaces in vitro. Adhesion to hydrocarbons was used for hydrophobicity assessment. Three strains of L. reuteri and L. murinus C were evaluated as hydrophobic, others as intermediate. All tested strains were able to form the biofilm on polystyrene. L. mucosae D and L. reuteri E were tested for adhesion to epithelial cell lines (HeLa and Caco-2). Both were more adherent to HeLa than to Caco-2. The adhesivity degree in HeLa reached the highest value after 8 h of co-cultivation in both lactobacilli tested, then decreased. In Caco-2, adhesion was increased within 24 h from the beginning of the co-cultivation. Mucus-binding protein gene, implicated in adhesion, was detected in L. mucosae D. Therefore, the involvement of proteinaceous substances in binding process was investigated. Cells of L. mucosae D were digested by three proteolytic enzymes (proteinase K, pronase E, trypsin) and evaluated for time-dependent adhesivity changes to HeLa, Caco-2, and L929 cell lines. Results confirmed that proteins are most likely to play an important role in binding of lactobacilli to eukaryotic cells. One hour after treatment, L. mucosae D was able to overcome the effect of proteolytic cleavage. We assume that it was due to the restoration of its cell-surface binding structures. Co-cultivation of HeLa and L. mucosae D led to protuberance and communication channels formation in eukaryotic cells.
Adhesive properties of food and faecal potential probiotic lactobacilli
Journal of Applied and Natural Science
In the present investigation, total four isolates of Lactobacillus species i.e. L. casei, L. helveticus, L. brevis and L. fermentum were examined for the cell surface hydrophobicity by bacterial adherence to hydrocarbons assay in LAPTg broth and hydrophobicity was calculated as percentage decrease in Optical Density at 600 nm. The general range of hydrophobicity in Lactobacilli was found in between 6-73%. Remarkably, L. helveticus and L . fermentum showed 73% hydrophobicity in xylene. Higher value of hydrophobicity could point toward a better ability of lactobacilli to adhere to epithelium cells. The outcome of present study concludes that L. helveticus and L. fermentum have good adhesive properties which may help them to adhere to surface epithelium of host cell and further screening with other probiotic attributes could be designated as probiotics.
Prebiotic Treatment Influence the Adhesion Properties of Three Lactobacillus strains
International Journal of Current Microbiology and Applied Sciences, 2017
Different species from the genus Lactobacillus are commonly used as probiotics (Zago et al., 2011). By definition, probiotics are live microorganisms that confer a health benefit on the host when administered in adequate amounts (FAO/WHO, 2001). Their efficacy has been demonstrated for the treatment of gastrointestinal disorders, respiratory infections and allergic symptoms (Wohlgemuth et al., 2010). The general mechanisms of action that induce these beneficial results are: production of antibacterial substances; induction of defensin production by intestinal epithelial cells; competitive exclusion of pathogenic bacteria; influence on host microbiota and pathogenic bacteria; improved intestinal barrier function; modulation of host immune (Wohlgemuth et al., 2010). The predominant number of species applied as probiotics belong to the group of lactic acid bacteria (LAB), named after the main end product of their carbohydrate metabolism. LAB are GramInternational Journal of Current Mic...
Good adhesion properties of probiotics: a potential risk for bacteremia
FEMS Immunology & …, 2001
The ability to adhere to human intestinal mucus was tested for lactic acid bacteria of clinical blood culture, human fecal and dairy origin. The blood culture isolates were found to adhere better than the dairy strains. Of the Lactobacillus rhamnosus strains (nine clinical, 10 fecal and three dairy), blood culture isolates adhered better than the fecal strains. Although these results indicate a trend for blood culture isolates to bind to intestinal mucus in higher numbers than strains of dairy and human fecal origin, other factors are also likely to be involved in the etiology of lactobacillemia since some of the clinical Lactobacillus isolates exhibited a relatively low level of adhesion.