Heavy metal biosorption potential of Aspergillus and Rhizopus sp. isolated from wastewater treated soil (original) (raw)
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BIOSORPTION OF HEAVY METALS USING ASPERGILLUS SPECIES ISOLATED FROM CONTAMINATED SOIL.
With the rapid development of industries heavy metal pollution has become one of the major global concerns due to their toxicity and threat to human life and environment. This work evaluated the heavy metal biosorption potential of Aspergillus sp. (Aspergillus flavus and Aspergillus fumigatus) isolated from contaminated soil of Bhagwanpur industrial area, Haridwar. The heavy metal concentrations were determined after digestion of soil samples. The results indicate the heavy metal resistant fungi that were isolated and screened for the biosorption potential. The minimum inhibitory concentration (MIC) of Pb, Cr, Ni and Zn was determined by agar diffusion method in 25, 50, 100, 200 and 400 ppm concentrations. In this study, adsorption of Zn, Ni, Cr and Pb were investigated and two sp. of Aspergillus were identified viz. Aspergillus flavus and Aspergillus fumigatus. The results showed that Aspergillus flavus and Aspergillus fumigatus could biosorb all the metals in the order Zn>Ni>Cr>Pb and Pb>Cr>Zn>Ni respectively.
Bioresource Technology, 2007
Heavy metal analysis of agricultural field soil receiving long-term (>20 years) application of municipal and industrial wastewater showed two-to five-fold accumulation of certain heavy metals as compared to untreated soil. Metal-resistant fungi isolated from wastewater-treated soil belonged to genera Aspergillus, Penicillium, Alternaria, Geotrichum, Fusarium, Rhizopus, Monilia and Trichoderma. Minimum inhibitory concentrations (MIC) for Cd, Ni, Cr, Cu, and Co were determined. The MIC ranged from 0.2 to 5 mg ml À1 for Cd, followed by Ni (0.1-4 mg ml À1 ), Cr (0.3-7 mg ml À1 ), Cu (0.6-9 mg ml À1 ) and for Co (0.1-5 mg ml À1 ) depending on the isolate.
Indian journal of experimental biology, 2006
Fungi including Aspergillus and Penicillium, resistant to Ni2+, Cd2+, and Cr6+ were isolated from soil receiving long-term application of municipal wastewater mix with untreated industrial effluents of Aligarh, India. Metal tolerance in term of minimum inhibitory concentration (MIC) was 125-550 microg/ml for Cd, 300-850 microg/ml for Ni and 300-600 microg/ml for Cr against test fungi. Two isolates, Aspergillus niger and Penicillium sp. were tested for their Cr, Ni and Cd biosorption potential using alkali treated, dried and powdered mycelium. Biosorption experiment was conducted in 100 ml of solution at three initial metal concentrations i.e., 2, 4 and 6 mM with contact time (18 hr) and pretreated fungal biomass (0.1g) at 25 degrees C. Biosorption of all metals was found higher at 4 mM initial metal concentration as compared to biosorption at 2 and 6 mM concentrations. At 4 mM initial metal concentration, chromium biosorption was 18.05 and 19.3 mg/g of Aspergillus and Penicillium bi...
Biosorption of heavy metal polluted soil using bacteria and fungi isolated from soil
SN Applied Sciences, 2019
Heavy metals polluted soils have turned out to be a common environmental problem across the globe due to their toxic effects and accumulation through the food chain. Heavy metals have lethal effects on all forms of life. For instance, plants grown on heavy metal polluted soil show a reduction in growth and yields. A surge in anthropogenic activities and industrial operations has substantially increased the level of heavy metal pollution and release into the environment; hence, there is need to remediate these heavy metal pollutants. Biosorption is an efficient, economical, ecofriendly and convenient techniques of remediating heavy metal polluted soils. It is a widely accepted method that utilizes biomaterials such as natural biomass as biosorbents. The current study was based on the biosorption of copper, chromium, cadmium and nickel polluted soil using bacteria and fungi isolated from soil. Bacterial species isolated were Pseudomonas, Bacillus, Micrococcus, Escherichia, Streptococcus, Enterobacter and Staphylococcus while fungi isolated were Aspergillus niger, Penicillium notatum and Aspergillus flavus. The isolated bacteria were screened for potential to biosorb copper and chromium likewise fungi for cadmium and nickel. Biosorption rate was determined using atomic absorption spectrophotometry. Five milliliters each of a-day-old culture of the screened bacteria and fungi was inoculated into 45 ml of nutrient broth (bacteria) and potato dextrose broth (fungi) having concentrations of 5, 10, 15 and 20 ppm, respectively, of copper, chromium, cadmium and nickel. The conical flasks were incubated at a temperature of 37 °C and 28 °C ± 2 for bacteria and fungi, respectively, for a period of 35 days of inoculation. For the bacterial isolates, the highest biosorption rates of chromium (89.67%) and copper (90.89%) by Pseudomonas aeruginosa were observed at 20 ppm on day 21 and 15 ppm on day 14, respectively, while for the fungi isolates, P. notatum showed highest biosorption rate for cadmium at 10 ppm with 77.67%. Aspergillus niger showed highest biosorption rate for nickel with 81.07% after 28 days of incubation. The results of this study revealed the ability of Pseudomonas aeruginosa to biosorb copper and chromium and also A. niger and P. notatum to biosorb cadmium and nickel from the environment and can be developed for the biosorption of soils polluted with copper, chromium, cadmium and nickel.
Biosorption of cadmium and nickel by pretreated Aspergillus spp. biomass
2019
Industrial effluents contaminated with the heavy metals pose threat to the environment and its habitants. Biosorption is an effective and eco-friendly method for sequestration of heavy metals from such effluents. Fungi, with their remarkable metabolism-independent metal uptake systems, are efficient natural biosorbents of heavy metals. Therefore, we explored fungal biomass (Aspergillus spp.) pretreated with formaldehyde (solvent) and sodium hydroxide (alkali) for sequestration of metals cadmium (Cd) and nickel (Ni) from the aqueous solutions contaminated with heavy metals. The results have shown significant increase in the sequestration of Cd and Ni by the Aspergillus spp. biomass pretreated with formaldehyde and sodium hydroxide and thereby demonstrated its potential in cleaning the environment polluted with heavy metals.
Biosorption of cadmium and copper by Aspergillus spp. isolated from industrial ceramic waste sludge
Biological Diversity and Conservation, 2019
Under proper conditions, fungi can act as a good biosorbent for different heavy metals. In the present study, Aspergillus spp. have been isolated from ceramic industrial waste sludge and the tolerance of the fungi for copper and cadmium metals were examined. The experiments were carried out at 25 °C , pH=4-4.5 for Cu(II), pH=6 for Cd(II), biosorbent dose of 2.5 g, initial metal concentration of Cd (II) was 1 mM and Cu(II) was 5 mM. The removal efficiencies for cadmium and copper with two Aspergillus strains were found to be 90-95% and 85-90%, respectively. The sorption capacities of live and dead fungi for copper were 5.3676 mg g-1 , 18.661 mg g-1 and for cadmium were 0.1977 mg g-1 , 0.1772 mg g-1 respectively. FTIR analyses have showed that copper ions bound to vinyl compounds (950-900 cm-1) and cadmium ions bound to primer amides (1420-1400 cm-1), mostly. Considering biosorption results, Langmuir and Freundlich isotherm models have been described and it was clearly seen that none of the isotherm models have fitted the experimental data. The metal ion binding areas of the cell surface of fungi were determined by FTIR. SEM monitoring and EDX analysis were carried out. EDX results confirmed the biosorption of copper and cadmium.
Heavy metal biosorption by white-rot fungi
Water Science and Technology, 1998
In this study. heavy metal biosorption potentials of two white-rot fungi. PolyporolU versicolor and Phanaroc#UJete chrysosporium. which are commonly used in wastewater treatment were determined. Biosorption studies were performed for Cu{Il). Cr(III). Cd(lI). Ni(lI) and Pb{II) al the lAIIIe operational conditions and the effectiveness of bothfungi al removing theseheavy metals wascompared. II was found that both P. "enicolor and P. chrysosporium were the most effective in removing Pb(II) from aqeoua solutions with maximum biosorption capacities of 57.Sand 110 mg Pb(II)fg dry biomass. respectively. With P. versicolor, the adsorptive capacity order was determined to be Pb(II»Ni(ll)>Cr(m)>Cd(U)>Cucm whereas the orderwasPb(U)>Cr(III)>Cu(1I):CdCIl)>Ni{Il) withP. chrysosporium. AI a general trend,metal removal efficiency with these fungi decreased as the initial metal ion concentration increased. Q 1998 Published by ElsevierScience Ltd. Allrights reserved
Biosorption of cadmium, manganese, nickel, lead, and zinc ions by Aspergillus tamarii
In this paper, Cd 2+ , Mn 2+ , Ni 2+ , Pb 2+ , and Zn 2+ heavy metals ions adsorption properties of commercially obtained Aspergillus tamarii were investigated. The dead biomass was used with a batch system for experiments. The effect of the operating parameters, such as pH, temperature, agitation speed, contact time, initial metal concentration, and biomass dosage of aqueous solution containing Cd 2+ , Mn 2+ , Ni 2+ , Pb 2+ , and Zn 2+ was studied to find biosorption capacity. The optimum pH range for all heavy metal uptakes was 6.0. The experiments were carried out at different temperatures in the range of 20-50˚C and the maximum uptake was found to be at 25˚C. Heavy metal ion uptake increased with agitation speed until 150 rpm. After this agitation speed adsorption capacity slightly decreased. The adsorption equilibrium was obtained at 150 min contact time. At the optimal conditions, maximum uptake of Cd 2+ , Mn 2+ , Ni 2+ , Pb 2+ , and Zn 2+ was found to be 51. 69, 46.99, 58.74, 98.14, and 54.33%, respectively, by using 1.5 g biomass. The interaction between heavy metals and biomass was characterized by FTIR spectroscopy.
Scientific Journal for Damietta Faculty of Science, 2016
This work was conducted to study the effect of some environmental conditions on bioremoval of heavy metals from industrial wastewater by consortium cultures of Aspergillus niger and A. flavus. Many procedures on the effect of incubation period, temperature, pH and concentration of metals were carried out in the laboratory. Results proved that the high culture growth and maximum heavy metals removal were after 21 days incubation at 30 o C, pH of 5.4 and metal concentration at 10 ppm. While the lowest culture growth and minimum heavy metals removal were after 7 days incubation at 5 o C , pH of 10.8 and metal concentration at 100 ppm.
Biosorption of Copper and Lead by Heavy Metal Resistant Fungal Isolates
Microorganisms play a significant role in bioremediation of heavy metal contaminated soil and wastewater. In this study heavy metal resistant fungi were isolated from the waste water treated soil samples of Hudiara drain, Lahore. The optimum pH and temperature conditions for heavy metal removal were determined for highly tolerant isolates of Aspergillus species along with the initial metal concentration and contact time. Biosorption capacity of Aspergillus flavus and Aspergillus niger was checked against Cu (II) and Pb (II) respectively. The optimal pH was 8-9 for A. flavus and 4-5.4 for A. niger, whereas the optimal temperature was 26°C and 37°C respectively. Moreover, the biosorption capacity of A. flavus was 20.75-93.65 mg/g for Cu (II) with initial concentration 200-1400 ppm. On the other hand the biosorption capacity of A. niger for Pb (II) ranged from 3.25-172.25 mg/g with the same range of initial metal concentration. It was also found that equilibrium was maintained after maximum adsorption.