Assessment of Lipid Peroxide and Lipid Profiles in Patients with Essential Hypertension (original) (raw)
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Lipid peroxidation the levels of antioxidant enzymes in hypertension
Free Radicals and Antioxidants, 2012
There is substantial evidence that oxidative stress plays a major role in hypertension and subsequently the atherosclerotic process. The aim of the present study was to investigate the presence of oxidative stress in hypertension. Materials and Methods: The study included 46 hypertensive and 48 control subjects. Superoxide dismutase, glutathione, glutathione peroxidase activity and malondialdehyde level were measured in serum samples of the participants along with fasting lipid levels. Statistical Analysis Used: All data was entered into an Excel spreadsheet and analyzed using standard statistical software like Statistical Package for the Social Sciences (SPSS). Chi square test was used for categorical variables. Results: Serum malondialdehyde level was significantly raised in the study group as compared to control group (p < 0.05). Superoxide dismutase activity, whole blood glutathione levels and glutathione peroxidase activity were significantly decreased in all the subgroups of study group as compared to control group (p < 0.05). Cholesterol, low density lipoproteins and triglycerides showed significant rise, whereas high density lipoprotein was decreased as compared to normal. Conclusions: The higher malondialdehyde level and lower activity levels of other antioxidant molecules measured in this study could have resulted from increased free radical generation, which may confirm the presence of oxidative stress in hypertension. However, further elaborate clinical studies are required to evaluate the role of such antioxidant enzymes.
Biomarkers of lipid peroxidation related to hypertension in aging
Hypertension research : official journal of the Japanese Society of Hypertension, 2016
The aim of this clinical study was to evaluate the influence of aging on the levels of lipid peroxidation (quantified as thiobarbituric acid-reactive substances (TBARS) content), lipid hydroperoxide (LOOH), hexanoyl lysine (HEL), 8-iso-prostaglandin F2α (8-iso-PGF2α) and total antioxidant capacity (TAC), and determine their relationships to the demographic and cardiovascular risk factors in elderly hypertensive (HT) patients. This study consisted of four groups: two elderly groups with 30 HT patients (11 males, 19 females) and 30 normotensive healthy volunteers (15 males, 15 females), and two young groups with 30 HT patients (13 males, 17 females) and 30 normotensive healthy volunteers (12 males, 18 females). In the elderly control group, the TBARS, LOOH, HEL and 8-iso-PGF2α levels, and the carotid intima media thickness (CIMT) were significantly higher than in the young control group. The TBARS, LOOH, HEL and 8-iso-PGF2α levels and the CIMT measurements were significantly higher in...
2018
DOI: 10.21276/sjams.2018.6.7.30 Abstract: The present study was carried out to estimate the erythrocyte glutathione peroxidase activity, an antioxidant enzyme and malondialdehyde (marker of lipid peroxidation) in Hypertensive subjects of different grades as per JNC 7 th norms and to determine their relation with each other in essential hypertensive patients. In the present study, erythrocyte glutathione peroxidase activity and malondialdehyde levels were measured in 90 hypertensive subjects (30-60 years), categorized into three groups as prehypertension, Stage I HT and Stage II HT, depending upon their blood pressure and statistically compared it with that of 30 healthy individual, served as control. Correlation analysis between aforesaid parameters was performed by using Pearson correlation test. Erythrocyte glutathione peroxidase activity was found to be significantly low in each patient group as compared to control (P < 0.001) where as malondialdehyde levels were increased sig...
Oxidant–antioxidant Status and Lipid Profile in the Hypertensive Patients
Journal of Nepal Health Research Council, 2009
Background: Hypertension is associated with an elevation of ROS and frequently also with an impairment of endogenous antioxidant mechanisms. Increased level of serum cholesterol, TG, VLDL has been observed in patients with hypertension. It has been shown that oxidized lipoprotein inactivates NO and aggravates hypertension. This study intends to know the association of oxidative stress and lipid profile with hypertensive patients of the Western Nepal.
Lipid Concentration And The Extent Of Their Peroxidation In Nigerian Hypertensives
2007
Plasma total cholesterol, triglycerides, high density lipoprotein cholesterol, low density lipoprotein cholesterol, Phospholipids and molonydialdehyde were determined in normotensives, hypertensives on anti – hypertensive drugs and hypertensives not on any anti hypertensive drugs aged between 30 – 60 years. Plasma samples were used. Result were statistically significant in all parameters studied at the level of (p < 0.001) in normotensives, hypertensives on drugs and hypertensives not on drugs except in phospholipids where there was no statistical difference. Relationship between blood pressure and lipid concentration were established. The product of lipid peroxidation, malonydialdehyde was statistically significant in hypertensives on anti-hypertensive drugs and hypertensives not on any anti-hypertensive drugs when compared to normotensive control at (p<0.001) indicating free radical generation as a result of oxidative stress. These finding indicates that there are high lipid...
BLOOD PRESSURE, LIPID PROFILE AND LIPID PEROXIDATION IN DIABETIC HYPERTENSIVE PATIENTS
Background and Objective: Hypertension is correlated with the incidence of atherosclerosis. Hence, in the present work was undertaken to investigate the association of Blood pressure, Lipid profile and lipid peroxidation in hypertensive type II Diabetes Mellitus patients. Materials and Methods: In this study 40 cases of borderline to mild essential hypertensive subjects of age group 45–69 years and 40 healthy age and sex matched subjects were used. Patients were selected as per the detailed history and routine examination and were excluded if any secondary cause of hypertension or any chronic complication. Systolic (SBP) and diastolic blood pressure (DBP) of all subjects were recorded. 12 hours fasting blood samples were collected from all subjects for biochemical analysis. Lipid profile and Fasting Blood Glucose were measured by enzymatic methods in semiautoanalyzer. Serum MDA was measured by spectrophotometric method. The data were analyzed for Statistical significance using one way ANOVA and P<0.05 was the level of significance. Results: There was a significant increase (p=0.0001) in the FBS level, SBP, DBP, Total cholesterol, Triglyceride level and LDL-Cholesterol level in Non-Diabetic Nonhypertensive Controls. The MDA level was significantly increased (p=0.05) in Diabetic hypertensive patients. Conclusion: Our study revealed that Lipid profile, MDA and Blood Sugar has interactive connection with type II diabetic mellitus. It is therefore recommended that healthcare providers should consider testing diabetic patients for complications due to hyperlipidemia as a part of the treatment.
Evidences for oxidative stress in essential hypertension
Central European Journal of Medicine, 2012
Aim: This study explores the degree of oxidative stress in essential arterial hypertension (EAH). Even oxidative stress appears as one of several metabolic abnormalities involved in essential hypertension, it remains uncertain whether is primary or secondary. However measurement of the main oxidant may be useful in order to recognize and monitor oxidative stress. Methods: Lipid peroxidation products (TBA reac tive substances) were determined in red blood cells (RBC) and serum together with markers of antioxidant status: total antioxidative capacity (AOC), catalase activity (CAT) and RBC glutathione (GSH) content in four investigated groups. The first group consisted of regularly and adequately treated hyper tensive patients without complication (regulated), patients with hyper tension and complication of some organs and organic systems were the non-regulated group. Third group were patients at the beginning of EAH (non-treated) and controls were normotensive individuals. Results: Compared with controls, regulated and non-treated group, non-regulated hyper tensive pati ents had higher TBARS concen tration in plasma (p<0.001) and lower AOC, CAT activity without differences in GSH content. Conclusion: Recent findings suggest that hyper tension is a condition followed by intensive oxidative stress and reduced antioxidant factors. Adequate and strictly controlled therapy, hygienic and diet regime and possible use of antioxidants can signi fi cantly reduce blood pressure and prevent possible complications.
Study of Oxidative Stress in Essential Hypertension
Biomedical and Pharmacology Journal, 2015
Essential hypertension is associated with an elevation of reactive oxygen species (ROS) which reacts with membrane lipids to form lipid hydroperoxides that decomposes to form Malondialdehyde (MDA) an indicator of oxidative stress. Endogenous antioxidant enzyme, Superoxide dismutase (SOD) counteracts oxidative stress. This study aims at understanding the role of oxidative stress in essential hypertension. The study comprised of 50 confirmed cases of hypertension and 50 age and sex matched controls .Inclusion criteria includes patients with blood pressure e" 140/90 mm of Hg, while patients with secondary hypertension, stroke, CAD,MI and diabetes mellitus are excluded. Serum SOD estimated spectrophotometrically by Mishra H.P. & Fridovich I,1972 method and Plasma MDA by colorimetric method of Satoch K.et al. SOD activity was statistically significantly (p<0.0001) decreased while MDA level was statistically significantly (p<0.0001) increased in hypertensives compared to controls. Patients suffering from hypertension have increased ROS activity which oxidizes nitric oxide (NO) and affect vascular tone.Lassegue et al (2004) also found convincing evidence that ROS is an intrinsic part of pathology of hypertension. If oxidative stress is indeed a cause or consequence of hypertension, then reduction in oxidative damage may result in a reduction in blood pressure. Antioxidants like Vit.A,Vit.C & Vit E which are present in vegetables, citrus fruits & oils respectively are able to trap ROS and thus may be capable of reducing oxidative damage and possibly blood pressure. Estimation of oxidative stress markers (SOD & MDA) is simple and inexpensive; it can be used to predict the development of atherosclerotic disease like coronary artery disease, cerebrovascular disease and renal complications associated with essential hypertension. Oxidative stress markers estimation may also be helpful in assessing the usefulness of antihypertensive drugs in prevention of associated complications.
Oxidative Stress in the Elderly with Hypertension: A Cross-Sectional Study
2021
Hypertension is a major health problem, especially in the elderly, because it serves as a risk factor for cardioand cerebrovascular diseases. The involvement of oxidative stress in hypertension has been shown in animal studies. However, the data about oxidative stress in humans with hypertension, especially in the elderly, are still limited. The aim of this study is to analyze oxidative stress by measuring carbonyl and superoxide dismutase (SOD) in hypertensive elderly. It was a cross-sectional study conducted on 70 elderly subjects, 35 subjects with hypertension and 35 subjects with normotension, in Jakarta, Indonesia. Subjects were classified into the hypertensive group if their systolic blood pressure was ≥ 130 mmHg or their diastolic blood pressure was > 80 mmHg according to American guidelines. Plasma carbonyl and SOD were measured using a spectrophotometer. Correlation analysis and Independent T-test were used for statistical analysis. Carbonyl was significantly higher, whi...
Relationship between Oxidative Stress and Essential Hypertension
Hypertension Research, 2007
This study investigated the association of blood pressure with blood oxidative stress-related parameters in normotensive and hypertensive subjects. A cross-sectional design was applied to 31 hypertensive patients and 35 healthy normotensive subjects. All subjects were men between the ages of 35 and 60 years. Exclusion criteria were obesity, dyslipidemia, diabetes mellitus, smoking and current use of any medication. All patients underwent 24-h ambulatory blood pressure monitoring and sampling of blood and urine. Antioxidant enzymes activity, reduced/oxidized glutathione ratio (GSH/GSSG), and lipid peroxidation (malondialdehyde) were determined in erythrocytes. Parameters measured in the plasma of test subjects were plasma antioxidant status, lipid peroxidation (8-isoprostane), plasma vitamin C and E, and the blood pressure modulators renin, aldosterone, endothelin-1 and homocysteine. Daytime systolic and diastolic blood pressures of hypertensives were negatively correlated with plasma antioxidant capacity (r =-0.46, p <0.009 and r =-0.48, p <0.007), plasma vitamin C levels (r =-0.53, p <0.003 and r =-0.44, p <0.02), erythrocyte activity of antioxidant enzymes, and erythrocyte GSH/GSSG ratio, with hypertensives showing higher levels of oxidative stress. Blood pressures showed a positive correlation with both plasma and urine 8-isoprostane. Neither plasma vitamin E nor the assessed blood pressure modulator levels showed significant differences between the groups or correlation with blood pressures. These findings demonstrate a strong association between blood pressure and some oxidative stress-related parameters and suggest a possible role of oxidative stress in the pathophysiology of essential hypertension. (Hypertens Res 2007; 30: 1159-1167)