Study of Oxidative Stress in Essential Hypertension (original) (raw)
Related papers
Study of Oxidative Stress Parameters in Hypertension and their Correlation with Blood Pressure
2014
Increasing evidence has underlined the importance of oxidative stress in the Pathophysiology of hypertension and its contribution to the associated complications. However the correlation between oxidative stress parameters and blood pressure levels is not clearly understood. Thus present study was done to evaluate the oxidative stress parameters in patients with hypertension and to check the correlation, if any, between these parameters and systolic and diastolic blood pressure. Plasma malondialdehyde, superoxide dismutase and catalase levels were measured in sixty hypertensive and forty normotensive subjects. Pearson’s correlation analysis was performed to study the correlation between these parameters and systolic and diastolic blood pressure. Enhanced oxidative stress was observed in hypertensive subjects as denoted by raised plasma malondialdehyde levels and reduced superoxide dismutase and catalase levels. Plasma malondialdehyde correlated positively whereas superoxide dismutas...
Oxidative Stress Markers and Antioxidant Status in Human Hypertension
2011
Background: There is growing evidence that oxidative stress contributes to hypertension justified by observations, predominantly in animal models. Objective: The aim of the present study was to observe the relationship, if any, between systemic arterial hypertension and biochemical markers of oxidative stress, inflammation and aging. Materials & Methods: 50 subjects with hypertension and 50 age and sex-matched controls were included in the study. The nitrate-nitrite ratio, malondialdehyde (measured as thiobarbituric acid reactive substances, superoxide dismutase activity, and high sensitive C-Reactive Protein were assayed on samples from these subjects by standardized methodology. The data obtained was statistically analyzed. Results: Statistically significant mean values of serum nitrate-nitrite ratio and plasma superoxide dismutase activity were lower and that of serum malondialdehyde was higher in hypertensive subjects when compared to the controls. Serum nitrate-nitrite ratio, malondialdehyde and superoxide dismutase activity exhibited statistically significant multivariate correlation with each other while High Sensitive C-Reactive Protein was not significantly correlated. Nitrate-nitrite ratio was significantly lower with aging both in normotensive and hypertensive subjects. Conclusion: The present study confirmed the role of oxidation mediated tissue damage in generation of hypertension and suggested the usefulness of examining nitrate:nitrite ratio as a surrogate marker of the pathophysiologic process leading to generation of human hypertension. The study also confirmed an exacerbation of oxidative stress with advancement of age in both study groups.
Evidences for oxidative stress in essential hypertension
Central European Journal of Medicine, 2012
Aim: This study explores the degree of oxidative stress in essential arterial hypertension (EAH). Even oxidative stress appears as one of several metabolic abnormalities involved in essential hypertension, it remains uncertain whether is primary or secondary. However measurement of the main oxidant may be useful in order to recognize and monitor oxidative stress. Methods: Lipid peroxidation products (TBA reac tive substances) were determined in red blood cells (RBC) and serum together with markers of antioxidant status: total antioxidative capacity (AOC), catalase activity (CAT) and RBC glutathione (GSH) content in four investigated groups. The first group consisted of regularly and adequately treated hyper tensive patients without complication (regulated), patients with hyper tension and complication of some organs and organic systems were the non-regulated group. Third group were patients at the beginning of EAH (non-treated) and controls were normotensive individuals. Results: Compared with controls, regulated and non-treated group, non-regulated hyper tensive pati ents had higher TBARS concen tration in plasma (p<0.001) and lower AOC, CAT activity without differences in GSH content. Conclusion: Recent findings suggest that hyper tension is a condition followed by intensive oxidative stress and reduced antioxidant factors. Adequate and strictly controlled therapy, hygienic and diet regime and possible use of antioxidants can signi fi cantly reduce blood pressure and prevent possible complications.
Relationship between Oxidative Stress and Essential Hypertension
Hypertension Research, 2007
This study investigated the association of blood pressure with blood oxidative stress-related parameters in normotensive and hypertensive subjects. A cross-sectional design was applied to 31 hypertensive patients and 35 healthy normotensive subjects. All subjects were men between the ages of 35 and 60 years. Exclusion criteria were obesity, dyslipidemia, diabetes mellitus, smoking and current use of any medication. All patients underwent 24-h ambulatory blood pressure monitoring and sampling of blood and urine. Antioxidant enzymes activity, reduced/oxidized glutathione ratio (GSH/GSSG), and lipid peroxidation (malondialdehyde) were determined in erythrocytes. Parameters measured in the plasma of test subjects were plasma antioxidant status, lipid peroxidation (8-isoprostane), plasma vitamin C and E, and the blood pressure modulators renin, aldosterone, endothelin-1 and homocysteine. Daytime systolic and diastolic blood pressures of hypertensives were negatively correlated with plasma antioxidant capacity (r =-0.46, p <0.009 and r =-0.48, p <0.007), plasma vitamin C levels (r =-0.53, p <0.003 and r =-0.44, p <0.02), erythrocyte activity of antioxidant enzymes, and erythrocyte GSH/GSSG ratio, with hypertensives showing higher levels of oxidative stress. Blood pressures showed a positive correlation with both plasma and urine 8-isoprostane. Neither plasma vitamin E nor the assessed blood pressure modulator levels showed significant differences between the groups or correlation with blood pressures. These findings demonstrate a strong association between blood pressure and some oxidative stress-related parameters and suggest a possible role of oxidative stress in the pathophysiology of essential hypertension. (Hypertens Res 2007; 30: 1159-1167)
Different antioxidants status, total antioxidant power and free radicals in essential hypertension
Molecular and Cellular Biochemistry, 2005
Hypertension is a multi-factorial process, prevalent in developed as well as in developing countries. Different antioxidants and free radicals play an important role in cardiovascular system. In present study, total antioxidant power in terms of FRAP (ferric reducing activity of plasma), free radicals and different antioxidants have been studied in essential hypertensives (n = 50) and normal subjects (n = 50). Levels of total cholesterol, low-density lipids-cholesterol, malonialdehyde, very low-density lipids (VLDL), uric acid, plasma homocysteine and low-density lipids (LDL), were significantly higher in hypertensives as compared to normotensive. HDL-cholesterol, SOD, GPx, reduced glutahione, total glutathione, oxidized glutathione, total thiols, protein thiols, non protein thiols, RNI, total antioxidant power, vitamin A, ascorbic acid and glutahione-S-transferase (GST) were decreased significantly in normotensive. We observed significantly low nitric oxide levels in hypertensive patients. No correlation was observed between severity of disease and plasma nitric oxide levels. There was a significant decrease in plasma FRAP value in essential hypertensives as compared to normotensive controls, which showed a negative correlation with diastolic blood pressure. In conclusion, our study revealed that there was a consistent significant difference between essential hypertensives versus controls with respect to most of the parameters. These complex changes are consistent in the view that essential hypertension is associated with an abnormal level of antioxidant status compared to normal response to oxidative stress or both. (Mol Cell Biochem 277: 89-99, 2005) oped as well as developing countries with a common end result of elevated blood pressure (BP). Hypertension is present in 60 to 70% of the population over 60 years of age and may result in cardiovascular complications such as stroke, coronary heart disease (CHD), and heart failure. High blood pressure (essential hypertension) is defined as systolic pressure (SBP) greater than 140 and/or diastolic pressure (DBP)
Antioxidant Status, Oxidative Stress and Lipid Profile in Essential Hypertensive Men
Journal of Evolution of Medical and Dental Sciences, 2013
Hypertension is a major health burden and coexists of with imbalance in antioxidants and lipid profile. This study is done to evaluate the total antioxidant status and oxidative stress like Malondialdehyde (MDA), catalase, superoxide dismutase (SOD), uric acid and correlate with lipid profile among hypertensive men. Significant increase in MDA, uric acid and dyslipidemia was found among hypertensive men. Also total antioxidant status and other antioxidants like SOD, catalase was found to be decreased in hypertensive men. Hence it is concluded that lipid peroxidation occur in hypertension which leads to endothelial dysfunction, renal blood flow alteration and tissue damage. As a result of which dyslipidemia, hyperuricemia and decrease in anti oxidants are seen. Hence decrease in antioxidants is a useful marker for antioxidant therapy to prevent organ damage among hypertensive.
Lipid peroxidation the levels of antioxidant enzymes in hypertension
Free Radicals and Antioxidants, 2012
There is substantial evidence that oxidative stress plays a major role in hypertension and subsequently the atherosclerotic process. The aim of the present study was to investigate the presence of oxidative stress in hypertension. Materials and Methods: The study included 46 hypertensive and 48 control subjects. Superoxide dismutase, glutathione, glutathione peroxidase activity and malondialdehyde level were measured in serum samples of the participants along with fasting lipid levels. Statistical Analysis Used: All data was entered into an Excel spreadsheet and analyzed using standard statistical software like Statistical Package for the Social Sciences (SPSS). Chi square test was used for categorical variables. Results: Serum malondialdehyde level was significantly raised in the study group as compared to control group (p < 0.05). Superoxide dismutase activity, whole blood glutathione levels and glutathione peroxidase activity were significantly decreased in all the subgroups of study group as compared to control group (p < 0.05). Cholesterol, low density lipoproteins and triglycerides showed significant rise, whereas high density lipoprotein was decreased as compared to normal. Conclusions: The higher malondialdehyde level and lower activity levels of other antioxidant molecules measured in this study could have resulted from increased free radical generation, which may confirm the presence of oxidative stress in hypertension. However, further elaborate clinical studies are required to evaluate the role of such antioxidant enzymes.
This study was undertaken to evaluate the serum levels of Oxidant (MDA) & antioxidant (SOD & Vitamin E) and compare oxidative stress (MDA) level among normotensive and hypertensive subjects. Oxidative stress has been relationship with mechanisms of EH (essential hypertension). A total number of 70 subjects were taken including both sex (Men and Women) between the ages of 35-70 years taken in this study. Exclusion criteria were chronic diseases, alcohol consumer, obesity, smoking/tobacco consumer and current use of any medication. Antioxidant enzymes activity and lipid peroxidation (malondialdehyde) were determined in serum. In 70 subjects out of 35 were found as an controls normotensive individuals and the cases 35 hypertensive patients. Serum MDA levels were highly significantly elevated in hypertensive patients in compared to normotensive individuals (4.39±0.98 µmol/l vs 1.51±0.70µmol/l and p < 0.0001). SOD acts as an antioxidant was highly significantly decrease in hypertensive patients in compared to normotensive individuals (0.44±0.06U/mg protein/min vs 0.96±0.04 U/mg protein/min and p <0.0001). Vitamin E, which acts as a biomarker of hypertensive was significantly higher decrease in hypertensive in compared to normotensive individuals (0.69±0.08 vs 1.06±0.25 and p < 0.001). These findings demonstrate the strong association of SOD and Vitamin E level decrease in hypertensive patients and by MDA level increase in hypertensive patients. Oxidative stress in hypertensive patients increasing over time may play a role in the improvement of atherosclerosis and cardiovascular disease, should be considered in further research.
Oxidant–antioxidant Status and Lipid Profile in the Hypertensive Patients
Journal of Nepal Health Research Council, 2009
Background: Hypertension is associated with an elevation of ROS and frequently also with an impairment of endogenous antioxidant mechanisms. Increased level of serum cholesterol, TG, VLDL has been observed in patients with hypertension. It has been shown that oxidized lipoprotein inactivates NO and aggravates hypertension. This study intends to know the association of oxidative stress and lipid profile with hypertensive patients of the Western Nepal.
Analysis Of Level Of Antioxidants In The Prognosis Of Hypertension ., Indo Am
The main objective of the study is to find the level of antioxidants in case of hypertension patients because hypertension directly effect on blood level and heart of the patient. Methodology of the study: This cross sectional study was conducted in Tehsil Headquarter Hospital Khairpur Tamewali Bahawalpur during January 2019 to August 2019. All the data was collected according to the rules and regulations of authority. The data was collected from both genders of age between 30 to 50years. The blood was drawn from all patients for further analysis of antioxidants. Results: Our results showed that the level of antioxidants increases in hypertension patients due to increase in blood flow. The level of MDA, SOD, GSH and CAT vary in a different manner. The level of SOD become decreases due to hypertension. Antioxidants are compounds that are able to trap ROS and thus may be capable of reducing oxidative damage and possibly blood pressure. Conclusion: In conclusion, we found that hypertension increased free radical levels in the blood. According to our study, levels of free radicals increase in the blood, which may stimulate antioxidant defense systems of body during hypertension.