A comparative study on the antioxidant activity of selected medicinal plant extracts (original) (raw)
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International Journal of Green Pharmacy, 2012
Background: Hopea ponga has been categorised as an endangered tree species under International Union for Conservation of Nature red list of threatened species. Objective: To study the antibacterial, antioxidant activities, total phenolic and flavonoid contents of Hopea ponga leaf, extracted from different solvents viz., petroleum ether, chloroform and methanol. Materials and Methods: Antibacterial activity of leaf extract of Hopea ponga was determined by disc diffusion method against Escherichia coli, Proteus vulgaris, Bacillus cereus, Streptococcus faecalis and Pseudomonas aeruginosa. The antioxidant activities of the extracts were assayed through in-vitro models such as 2,2-diphenyl-1-picrylhydrazyl, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid), ferric reducing antioxidant power, hydrogen peroxide and hydroxyl scavenging activities. Total flavonoid content was determined using aluminium chloride colorimetric method and total phenolic content determined using Folin ciocaulteu reagent method. Results: Methanol extract showed higher scavenging activity against 2,2-diphenyl-1-picrylhydrazyl and reducing power activity against ferric ion (97.24 ± 0.11 and 88.01 ± 0.03) compared to other extracts. Methanol extract showed significant antibacterial activity against P. vulgaris and B. cereus. Among the tested extracts, methanol extract exhibited higher content of phenols (1318.749 mg of ferulic acid equivalent/100 gram dry weight of extract) and flavonoids (457.832 mg of quercetin equivalent/100 gram dry weight of extract). Conclusions: The present study suggests that the methanol extract may contain active compounds, which have effective antioxidant and antimicrobial activity. Hence, this endangered species have to be explored and conserved to serve the mankind.
ANTIOXIDANT ACTIVITY EVALUATION OF KALANCHOE PINNATA (LAM.) PERS. FRESH LEAF JUICE
Asian Journal of Pharmaceutical and Clinical Research Journal, 2022
Objective: The objective of this study was to determine the antioxidant and free radical scavenging properties of Kalanchoe pinnata (Lam.) Pers. fresh leaf juice for standardized doses using different types of assays. Methods: The investigation of the antioxidant activity was carried out for the previously standardized doses (by cytotoxicity and genotoxicity analysis)-50 µl (low dose-LD) and 70 µl (high dose-HD) by Phosphomolybdenum (PM), Ferric Reducing Antioxidant Power (FRAP), 2, 2-diphenyl-1picrylhydrazyl (DPPH) radical scavenging activity, and 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity assays. Results: The activity measured by PM assay for LD and HD of the extract was equivalent to 109 µg/ml and 141 µg/ml of ascorbic acid, respectively, while by FRAP assay, it was equivalent to 17.5 µg/ml and 47 µg/ml of ascorbic acid, respectively. The activity measured by DPPH assay was found equivalent to 83 µg/ml and 177.5 µg/ml of ascorbic acid for LD and HD, with the percent scavenging activity 4.55% and 17.04%, respectively. Similarly, the activity of LD and HD by ABTS assay was found equivalent to 277.5 µg/ml and 308 µg/ml of ascorbic acid with the percent scavenging activity 39.01% and 47.93%, respectively. Conclusion: The changes observed in the absorbance values for all the assays reflected the increasing antioxidant activity of the plant extract in dosedependent manner, which justifies the traditional use of this herb in prevention of diseases induced by oxidative stress. The antioxidant activity of K. pinnata fresh leaf juice has been conducted for the 1 st time and has not been previously reported.
Free radical scavenging activities of different fractions of Kalanchoe pinnata
Aim and Objectives: In the present study, we have been successively extracted from methanolic extract of Kalanchoe pinnata into three successive different fractions like flavonoid rich fraction, saponin rich fraction and phenolics rich fraction to evaluate in-vitro models of antioxidant and anti-radical activities. Till date the correlation analysis between various parameters of Kalanchoe pinnata has not been reported. To our knowledge it may be first reported in the current research work. Material and Methods: Different fractions of Kalanchoe pinnata were extracted successively and were evaluated for their total phenolic and flavonoid contents. Antioxidative abilities of these fractions individually were analyzed by Phosphomolybdenum Assay (PMA), Cupric ions Reducing Antioxidant Capacity (CUPRAC) and Ferric ions Reducing Ability Power (FRAP) methods. The free radical scavenging activities such as hydroxyl, DPPH, and anti-peroxidation like Thiobarbituric Acid Reactive Substance (TBARS), Metal Chelation Capacity (MCC) were assayed through in-vitro models. Results: Antioxidant and antiradical effects of different fractions of Kalanchoe pinnata were ascertained through different in-vitro models. Results of individual tests demonstrated good correlation among themselves. In the correlation analysis, total flavonoids content has found to be negative correlation with all assays. Apart from total flavonoid contents, TBARS and DPPH assays have well correlated positively with other assays. Conclusion: Methanolic extract of Kalanchoe pinnata and its successive fractions were performed at various concentrations using in vitro models of antioxidant assays studied. From these results, revealed Kalanchoe pinnata exhibited significant antioxidant activity and free radical-scavenging activity. However, further evaluation of their isolated bioactive compounds, antioxidant activities should be investigated.
African Journal of Pure and Applied Chemistry, 2012
The research was conducted to determine the antioxidant activity of two Kalanchoe species in Ghana which are used widely for the treatment of various ailments including stroke and ulcers. The leaves were subjected to methanol and aqueous extractions for each sample. Thin layer chromatography (TLC) was performed to determine the various components in the leaves extract. The concentration of phenol and flavonoid was determined using folin-ciocalteu Zishen et al. (2003) and Lee et al. (2005) method. The extract was also screened to determine the total flavonoid and phenolic content of the methanol and aqueous extracts of the leaves of Kalanchoe pinnata and Kalanchoe integra and also to assess the antioxidant activity. 1,1-Diphenyl-2-picrylhydryzyl [DPPH] radical scavenging activity of both aqueous and methanol extracts of K. integra and K. pinnata were determined by the Brand-William method (2003)
Total antioxidant capacity (TAC) of fresh leaves of Kalanchoe pinnata
Herb Kalanchoe pinnata is grown as weeds in the tropical countries like India. It has a wide range of active constituents which has potent medicinal properties. The main objective was to overview the total antioxidant capacity of the herb Kalanchoe pinnata. Antioxidant activities were evaluated in terms of total phenolics content, total antioxidant activity, and reducing power. Different studies were carried out by comparing Kalanchoe pinnata extract with antioxidant references such as gallic acid; ascorbic acid. All these antioxidant activities increased with increasing concentrations in a dose dependent manner. It was found to be significant and valuable.
2021
The aim of this study was to analyze the chemical components and evaluate the biological activity of the extracts from the leaves Kalanchoe pinnata and Kalanchoe daigremontiana, which are cultivated in the province of Chiriqui, Republic of Panama. Phytochemicals components, antioxidant and anti-inflammatory activities were studied. The composition of the obtained petroleum ether, ethanol and aqueous extracts was analyzed by phytochemical screening. The antioxidant activity of the extracts was studied using three in vitro model systems (DPPH radical scavenging assay, nitric oxide radical scavenging assay, and superoxide radical scavenging activity). The anti-inflammatory activity of these species was studied using an in vivo model (ʎ-carrageenan-induced paw edema in rats). Phytochemical analysis of the extracts showed the presence of alkaloids, steroids, triterpenes, flavonoids, phenolic compounds, saponins and glycosides. The greatest radical inhibitory effect was observed in the DP...
Antioxidant and Free Radical Scavenging Activity of Certain Dietary and Medicinal Plant Extracts
The antioxidant and free radical scavenging activity of the ethanol extracts of Melia dubia Hiern., Albizia lebbeck (L.) Benth., Brassica oleracea L. var. botrytis (broccoli) and Glycyrrhiza glabra L. were evaluated. The total phenolic content of the extracts was found to be higher in A. lebbeck (33.6%) and lower in broccoli (16.9%). The antioxidant activity of the extract was evaluated through different chemical assays and in vitro methods such as reducing power, DPPH x , ABTS x+ and x OH radical scavenging activities, peroxidation inhibition in linoleic acid emulsion system and antihemolytic activity. The increasing concentrations of the extract exhibited nonlinear increases in the reducing power. Extract of A. lebbeck was found to have more hydrogen donating ability. Similar line of dose dependant activity has been observed in DPPH x and x OH radical scavenging systems. Further, A. lebbeck extracts exhibited strong antioxidant activity equivalent to trolox (6423.04 μmol g-1) in ABTS radical cation scavenging system. All the extracts exhibited notable activity against linoleic acid emulsion system (35.5 to 40.2%) and antihemolytic activity (29.8 to 46.6%).
Journal of Pharmacy and Nutrition Sciences, 2012
Many diseases are associated with oxidative stress caused by free radicals. Current research is directed towards finding naturally occurring antioxidants of plant origin. Kalanchoe crenata Andr. (Crassulaceae), commonly known as "never die" or "Dog's liver," has been traditionally used for the treatment of ailments, such as, earache, smallpox, headache, inflammation, pain, asthma, palpitations, convulsion, and general debility. The aim of present research deals with phytochemical screening and in-vitro evaluation of antioxidant activities of the leaves & stems of K.crenata. Method: Successive extracts of leaves & stems was subjected for phytochemical screening. The preliminary screening reports the presence of saponins, phytosterols, flavanoids, phenols and alkaloids in the extracts. Various extracts of K.crenata leaves & stems was studied in-vitro for total antioxidant activity, for scavenging of nitric oxide, hydrogen peroxide, the antioxidant capacity by p...
Screening of thirteen medicinal plant extracts for antioxidant activity
2010
Antioxidants are vital substances which possess the ability to protect the body from damage caused by free radical induced oxidative stress. A variety of free radical scavenging antioxidants exist within the body which many of them are derived from dietary sources like fruits, vegetables and teas. In this study the antioxidant activity and radical scavenging activity of methanolic extracts of selected plant materials, traditionally used by Iranian population as folk remedies was evaluated against linoleic acid peroxidation and 2,2diphenyl-1-picrylhydrazyl radical. The antioxidant activity expressed as IC 50 ranged from1.28 ng/ml in Biebresteinia multifida to 63.48 ng/ml in Polypodium vulgare. Radical scavenging activities expressed as IC 50 varied from 1.83 µg/ml in Salix sp. to 187.88 µg/ml in Allium hirtifolium.
Screening of medicinal plant extracts for antioxidant activity
Journal of Medicinal Plants …, 2009
The methanolic crude extracts of Desmodium gangeticum (Linn.), Eclipta alba (Linn.) Ocimum sanctum (Linn.), Piper longum (Linn.), Solanum nigrum (Linn.) and Amaranthus caudatus (Linn.) were screened for their free radical scavenging properties using ascorbic acid as standard antioxidant. Free radical scavenging activity was evaluated using 1,1-diphenyl-2-picryl hydrazyl (DPPH) free radical. The overall antioxidant activity of D. gangeticum was found to be the strongest, followed in descending order by A. caudatus, S. nigrum, P. longum, E. alba and O. sanctum. The IC 50 values of the extracts ranged between 0.05 ± 0 and 0.19 ± 0 mg/l . The ascorbic acid levels varied from 3.86 ± 0.20 to 21.33 ± 1.49 mg/100g and the carotenoids content were observed between 9.0 ± 0.24 to 24 ± 1.16 mg/100g in plant extracts. The highest total phenols content were found to be in O. sanctum (Linn.) with the value 48.93 ± 0.24 mg/g. The present study reveals that the selected plants would exert several beneficial effects by virtue of their antioxidant activity and could be harnessed as drug formulation.