Ubiquitin fusion augments the yield of cloned gene products in Escherichia coli (original) (raw)

Increasing gene expression in yeast by fusion to ubiquitin

Jonathan marsh

Journal of Biological Chemistry, 1989

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Expression and mutagenesis of human poly(ADP-ribose) polymerase as a ubiquitin fusion protein from Escherichia coli

Tauseef Butt

Biochemistry, 1991

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Molecular cloning and expression of a Tetrahymena pyriformis ubiquitin fusion gene coding for a 53-amino-acid extension protein

Paulo Guerreiro

Molecular and General Genetics, 1991

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Ultra-High Expression of a Thermally Responsive Recombinant Fusion Protein in E. coli

Ashutosh Chilkoti

Biotechnology Progress, 2006

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Introduction to Expression by Fusion Protein Vectors

Paul Riggs

Current Protocols in Molecular Biology, 2001

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High-Yield Expression in Escherichia coli and Purification of Mouse Ubiquitin-Activating Enzyme E1

Pedro Domingues

Molecular Biotechnology, 2012

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pUBEX/pUBSEX: a versatile expression vector system for production of fusion and nonfusion proteins in Escherichia coli

Keith Stanley

Gene, 1991

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Construction of a neo fusion gene for expression in both prokaryotic and eukaryotic cells

Adrian J Harwood

Gene, 1990

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Recombinant protein production data after expression in the bacterium Escherichia coli

Isaias Renteria

Data in Brief, 2016

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Expression of yeast deubiquitination enzyme UBP1 analogues in E. coli

Diana Mikiewicz

Microbial cell factories, 2005

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Multiple-copy genes: production and modification of monomeric peptides from large multimeric fusion proteins

Dave Plunkett

Gene, 1985

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Expression and accurate processing of yeast penta-ubiquitin in Escherichia coli.

Sobhanaditya Jonnalagadda

Journal of Biological …, 1987

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A Novel Cytokine Derived Fusion Tag For Over- Expression Of Heterologous Proteins In E. Coli

Sriram Padmanabhan

2009

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Depression of protein synthetic capacity due to cloned-gene expression inE. coli

Steven Peretti

Biotechnology and Bioengineering, 1990

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Advanced genetic strategies for recombinant protein expression in Escherichia coli

Kim Mortensen

Journal of Biotechnology, 2005

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Structure and expression of the Drosophila ubiquitin-80-amino-acid fusion-protein gene

A. Del Arco

The Biochemical journal, 1994

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Exploitation of GFP fusion proteins and stress avoidance as a generic strategy for the production of high-quality recombinant proteins

Christopher Hewitt, Yanina Sevastsyanovich, Jeff Cole

FEMS Microbiology Letters, 2009

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Expression of honeybee prepromelittin as a fusion protein in Escherichia coli

Brian Austen

Protein Expression and Purification, 1991

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Balanced expression of single subunits in a multisubunit protein, achieved by cell fusion of individual transfectants

Inger Sandlie

European Journal of Biochemistry, 2002

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Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression

Rajika L Perera

Bmc …, 2004

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Characterization of ubiquitin C-terminal hydrolase 1 (YUH1) from Saccharomyces cerevisiae expressed in recombinant Escherichia coli

Sun-Hee KIM

Protein expression and purification, 2007

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Production of active eukaryotic proteins through bacterial expression systems: a review of the existing biotechnology strategies

Sudhir Sahdev, Kulvinder Saini

Molecular and Cellular Biochemistry, 2007

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Structure and expression of the Drosophila ubiquitin-80-amino-acid fusion-protein gene

Rosa Barrio

Biochemical Journal, 1994

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Long-Term Stable and Tightly Controlled Expression of Recombinant Proteins in Antibiotics-Free Conditions

Seung-Goo Lee, Dae-Hee Lee

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Efficient expression of theEscherichia coli leuB gene in yeast

Reginald Storms

Current Genetics, 1985

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Recombinant protein expression in Escherichia coli

Eduardo A. Ceccarelli

Current Opinion in Biotechnology, 1999

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The UreEF Fusion Protein Provides a Soluble and Functional Form of the UreF Urease Accessory Protein

Robert Hausinger

Journal of Bacteriology, 2006

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