Isolation and Sequencing of Mycoplasma Arginini Genome Associated with Respiratory Disorder in Small Ruminants (original) (raw)
Related papers
Isolation and molecular characterization of Mycoplasma spp. in sheep and goats in Egypt
Veterinary World, 2019
Background and Aim: Different species of Mycoplasma are associated with many pathological problems in small ruminants including respiratory manifestation, this problem results in significant losses, especially in African countries. This study aimed to (I) study some epidemiological aspects of Mycoplasma species infections in Egyptian sheep and goats at Giza Governorate, (II) diagnosis of Mycoplasma species affections using bacterial isolation and identification, (III) apply the polymerase chain reaction (PCR) for typing of different Mycoplasma species, and (IV) illustrate the phylogenetic tree for the isolated Mycoplasma species and other species from GenBank using the purified PCR product. Materials and Methods: A total of 335 samples were collected from sheep and goats from Giza Governorate in Egypt as 142 nasal swabs from clinically affected animals, 167 pneumonic lungs, 18 samples from tracheal bifurcation, and 8 samples by bronchial wash were cultured on pleuropneumonia-like organisms (PPLOs) media for cultivation of Mycoplasma species. PCR and sequencing and phylogenetic analysis were adopted to identify and classify the isolated Mycoplasma species. Results: A total of 24 Mycoplasma isolates were isolated on PPLO media, identified by biochemical tests, and confirmed and typed by PCR using specific primers. 10 isolates were confirmed as Mycoplasma arginini, four isolates as Mycoplasma ovipneumoniae by PCR, and 10 isolates as undifferentiated Mycoplasma species. A purified isolate of M. arginini and M. ovipneumoniae was sequenced and phylogenetic analysis was illustrated. Conclusion: M. arginini and M. ovipneumoniae are prevalent in Egyptian sheep and goats. Further studies on M. arginini are required due to its high frequency of isolation from pneumonic sheep and goats and also from animals suffer from different respiratory manifestations.
Pakistan Journal of Zoology, 2015
Respiratory diseases of small ruminants are among the most important problems throughout the world as well as in Balochistan, Pakistan. Various Mycoplasma species lead to pneumonia and other respiratory diseases in sheep and goats and inflict heavy economic losses in Balochistan. The aim of present study was to highlight the prevalence of respiratory Mycoplasma species in nasal swab samples of sheep and goats through polymerase chain reaction (PCR) and further validation through Restriction Fragment Length Polymorphism (RFLP). In total, 240 nasal swab samples of Rakhshani breed of sheep and 200 nasal swab samples of Khurasani breed of goats were collected in 2011 from randomly selected sheep from Khanozai district, Pishin and goats from Kuchlak, district Quetta respectively. The extracted DNA samples were analyzed using the PCR for Mycoplasma mycoides cluster group, Mycoplasma mycoides sub-cluster group, Mycoplasma capricolum subspecies capripneumoniae (Mccp), Mycoplasma capricolum ...
Isolation of Mycoplasma species from respiratory infections of sheep and goats
The present study is carried out on isolation of Mycoplasma species from respiratory infections of sheep and Goats because Mycoplasma is also one of the major etiological agent in the production of respiratory infections which produces economic losses to small-scale farmers. In the present study a total of 188 Mycoplasma suspected samples, 95 (44 nasal swabs, 49 lung tissues and 2 pleural fluids) from sheep and 93 (56 nasal swabs, 33 lung tissues and 4 pleural fluids) from goats were collected. Isolation carried out on PPLO broth and PPLO agar. Positive samples produce color change in broth from brown to yellow and production of two different types of colonies on agar. One type is typical fried egg colony and other type is nipple-like with prominent tip. Conformation also done by PCR targeting Mycoplasma genus yielding 280 bp product size.
2018
Respiratory tract infections are the major cause of economic loss in goats. Mycoplasma agalactiae acts as an important etiological agent in mastitis, arthritis, kerato-conjuctivitis, pleuropneumonia and septicaemia syndrome (MAKePS). This paper reports a case study regarding the outbreak of respiratory disease associated with M. agalactiae in a herd of goats in Thrissur district, Kerala. The goats were presented with symptoms of respiratory distress, conjunctivitis, lethargy and reduced production. Nasal swabs were collected and dispensed in modified Hayfick’s broth without crystal violet. After incubation at 37°C for about 72 h., DNA was isolated from the broth and subjected to Mycoplasma genus specific PCR, which revealed amplicons of 280 bp confirming the genus. Also, the solid medium which was inoculated with filtered drops of incubated broth presented typical fried egg like colonies after five days of incubation and the isolates were identified as Mycoplasma by PCR. The species...
Veterinary World, 2021
Background and Aim: Mycoplasma infection in small ruminants is a serious problem in sheep and goat herds around the world. It is responsible for high economic losses and decreased animal productivity. This study aimed to highlight the clinical, histopathological, minimum inhibitory concentration (MIC), and molecular characterization of Mycoplasma species in sheep and goats in Menoufiya Governorate, Egypt. Materials and Methods: A total of 234 samples were collected; 104 samples were collected from pneumonic lung tissues from the abattoir, in addition, 10 and 20 samples collected from apparently and diseased sheep, respectively, and 40 and 60 samples were collected from apparently and diseased goats, respectively, which were subjected to isolation onto pleuropneumonia-like organism medium. Polymerase chain reaction (PCR), histopathological examination, and determination of the MIC were also performed. Results: Of 104 samples of lung tissues showing pneumonic lesions, 56 (53.84%) were positive for Mycoplasma isolation. The positive isolation of Mycoplasma from 10 and 20 samples from apparently and diseased sheep was 30% and 40%, respectively as well as the positive isolation of Mycoplasma was 17% and 56.66% out of 40 and 60 apparently healthy and diseased field goat's cases, respectively. All the diseased sheep and goats showed respiratory manifestations, including cough, bilateral nasal discharge, conjunctivitis, and systemic reaction. Evaluation of the MIC for Mycoplasma ovipneumoniae revealed that lincospectin and tylosin were the most effective antibiotics at 2.5 μg/mL. Histopathological examination of affected lung tissue showed extensive hemorrhagic pneumonia with extensive alveolar hemorrhage. The PCR technique proved to be a rapid, specific, and sensitive method for the detection of M. ovipneumoniae and Mycoplasma arginini at 390 and 326 bp, respectively. Conclusion: M. ovipneumoniae and M. arginini were the most prevalent species associated with respiratory infections in sheep and goats in the study area. Further studies are needed to investigate the role of these species in dissemination of the disease within herds of small ruminants.
Mycoplasma arginini: high frequency involvement in goat pneumonia
TURKISH JOURNAL OF VETERINARY AND ANIMAL SCIENCES
Mycoplasmas are an important class of bacteria associated with caprine pneumonia. Some of the main species involved are M. mycoides subsp. capri, M. capricolum subsp. capricolum, and M. capricolum subsp. capripneumoniae. In addition, there are frequent reports of M. arginini involvement. In the present study an attempt was made to see the association of various mollicutes in caprine pneumonia. In total 244 pneumonic goat lung samples collected over a 10-year period (2003-2012) were screened for the isolation and identification of associated mollicutes. The identification was done on the basis of biochemical tests, growth inhibition test, PCR, and sequencing. The most frequent isolates were Acholeplasma (20/244, i.e. 8.19%) and Mycoplasma arginini (13/244, i.e. 5.3%), followed by M. mycoides subsp. capri (1/244) and M. agalactiae (1/244). Seven representative isolates of M. arginini were subjected to partial sequencing of the rpoB gene. On phylogenic analysis, M. arginini isolates were found to be highly identical, exhibiting >98% identity with that of the standard M. arginini (ATCC 23243) strain. The results encourage exploration of the role of M. arginini in goat pneumonia in much detail, due to its high frequency involvement.
Pharma innovation, 2023
Infections of respiratory tract were one of the main problems in sheep and goats farming. Causing production and economic losses to farmers. Mycoplasma is one of etiological agent. Among all species, not only Mycoplasma mycoides cluster group but also non cluster organisms play important role in production of disease. In the present study a total of 188 Mycoplasma suspected samples, 95 (44 nasal swabs, 49 lung tissues and 2 pleural fluids) from sheep and 93 (56 nasal swabs, 33 lung tissues and 4 pleural fluids) from goats were collected. Molecular detection of genus Mycoplasma, Mycoplasma ovipneumoniae and Mycoplasma agalactiae was carried out by PCR targeting 16SrRNA yielding 280 bp, 361 bp and 360 bp product. The overall incidence of genus Mycoplasma was found to be 68.42% in sheep and 55.91% in goats. The overall incidence of Mycoplasma ovipneumoniae and Mycoplasma agalactiae was found to be 32.63% & 12.63% in sheep and17.2% &13.97% in goats.
2012
The present study highlights the prevalence of Mycoplasma species using PCR for Mycoplasma mycoides Cluster, Mycoplasma mycoides Sub-Cluster, Mycoplasma capricolum subspecies capripneumoniae (Mccp), and Mycoplasma putrefaciens (Mp) in 1920 nasal swab (DNA) samples of goats from five districts of Balochistan. Overall, 9.2% (n=177) prevalence of Mycoplasma species was observed. Districtwise prevalence of Mycoplasma species indicated statistically significant difference (X 2 =15.78, df = 4, P< 0.0033) with the highest prevalence of 14.1% in goats from Loralai district followed by 9.4% (Pishin), 8.3% (Zhob) and 8.1% (Killa Saifullah), while the lowest prevalence of 6.3% was noted in Quetta District. Of the total 177 (9.2%) nasal swab samples positive for Mycoplasma species, the highest prevalence of 69.5% (n=123) was found for Mmc followed by 19.2% (n=34) for Mcc, whereas the lowest prevalence of 11.3% (n=20) was observed for Mp organisms (X 2 =266.7, df=3, P<0.0001 indicated statistically highly significant difference). None of the DNA sample was found positive for Mccp organisms in specific PCR. The PCR result coupled with restriction fragment length polymorphism (RFLP) profile specifically indicated the presence of Mmc in goats in the studied districts of Balochistan. The use of PCR is found promising in the rapid detection of Mycoplasma species in the DNA extracted directly from the nasal swab samples of goats in Balochistan, Pakistan.
Pakistan Veterinary Journal, 2011
In this study, the presence of Mycoplasma ovipneumoniae and other mycoplasmas was investigated by culture and PCR in the nasal swab samples of 692 goats with nasal discharges in 44 different flocks in eastern Turkey. The results showed that approximately 41% (18/44) of the flocks and 11% (75/692) of the goats harbored Mycoplasma agents. Among the Mycoplasma species; M. ovipneumoniae was the most prevalent species detected within 29.5% (13/44) of the flocks and 8.1% (56/692) of the goats. Mycoplasma arginini was also detected solely or in combination with M. ovipneumoniae in 18.2% (8/44) of the flocks and 1.9% (13/692) of the goats with nasal discharges. None of the samples were determined to be positive in the M. mycoides cluster and Mycoplasma agalactiae specific PCR analyses. The remaining 12 samples could not be identified at species level. Because the investigation of other agents which may cause respiratory problems such as Mannheimia was beyond the scope of this study, it is n...
Assiut Veterinary Medical Journal, 2018
Mycoplasma ovipneumoniae (M. ovipneumoniae) was isolated from nasal swabs obtained from sheep with respiratory manifestations in delta region, Egypt; wherein 31 sheep with different ages were suffer from nasal discharge, cough, pneumonia, keratoconjunctivitis in a flock of sheep containing 134 sheep. By mycoplasma culture, nine samples were positive from 31examined samples (29%). M.ovipneumoniae is detected in 33.33% (3 out of 9 isolates of mycoplasma) using PCR with specific primer. The high percentage of isolation found in more than one-year age group, strains were isolated from the nasal swabs and no detection from ocular swabs. Isolated M. ovipneumoni strain subjected to sequencing and was designated as NMD-EG016, which showed a 94.6% 16S-23S RNA intergenic spacer sequence identity with three USA strains (M.