Slime production by bovine milk Staphylococcus aureus and identification of coagulase-negative staphylococcal isolates (original) (raw)
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International Journal for Modern Trends in Science and Technology, 2020
The present study was conducted to characterize the slime production activity of Staphylococcus aureus isolated from raw milk samples of various regions of Jaipur city of Rajasthan, India. Total 144 strains of S. aureus were confirmed by 23S rRNA amplification and 1250 bp product size on agarose gel confirmed S. aureus on molecular level. Slime production was determined by Congo red agar (CRA) method. Out of 144 S. aureus strains 134 (93.06%) produced slime and maximum slime production was found in Durgapura (100%) region followed by Mansarovar (95.65%) region and lowest slime production was found in Jhotwara area of Jaipur city of Rajasthan. This study provides an idea that infection of S. aureus may pose a potential risk to human health and these results may support the future actions related to milk safety programs.
South Asian Journal of Agriculture
Staphylococcus aureus is an emerging pathogen from dairy animals that can form biofilms in the udder of dairy cows affected by mastitis. The use of antibiotics in dairy farms leads to a decrease in bacteria, finally making healthy food. The aim of this study was to determine the occurrence, antibiotic susceptibility, and biofilm forming ability of S. aureus isolated from cow milk. A total of fifteen raw milk samples were collected from Mohammadpur, Narayangonj, Savar, Bosila, Siddheshwari and Norshingdi dairy farms for isolation of S. aureus. All the samples were highly loaded with Staphylococcus and the total staphylococcal count ranged from 105 to 106 cfu ml-1. Biochemical tests (TSI, catalase, oxidase, motility, MR/VP etc.) were performed for the confirmation of the Staphylococcal isolates. The 100% resistance was observed against oxacillin, erythromycin, cotrimoxazole, amoxicillin, cefixime and vancomycin. In contrast to these results, the isolates showed 100% sensitivity to gen...
SCISCITATIO
Food poisoning caused by the contamination from Staphylococcus aureus are frequently found in foodespecially in dairy products. Pasteurization process in milk production was not enough to kill S. aureus becauseit formed biofilm that could survive in high temperature. This research aimed to study the presence of biofilmforming S. aureus in samples from packed commercial milk products in Yogyakarta City, Indonesia. Twentyisolates from dairy products were grown in Brain Heart Infusion (BHI) broth then inoculated into Braid-ParkerAgar (BPA) medium to get the candidate of S. aureus isolates. These isolate candidates were selected usingMannitol Salt Agar (MSA) and Congo Red Agar (CRA) medium. Another selection was done by carbohydratefermentation analysis and confirmed using API STAPH. Confirmation analysis showed that eight isolateswere identified as S. aureus. Another two isolates were identified as S. xylosus and S. haemolyticus. Therefore,it indicated the presence of Staphylococcus au...
The study was carryout to evaluate the incidence and multidrug resistant of Staphylococcus aureus in different milk samples. Staphylococcus aureus is a major problem of public health which causes a number of human and animal diseases. The main source of infection is contaminated milk. Totally 50 raw and pasteurized milk samples were collected from three different sources such as Goat milk (15), Buffollow milk (25), and pasteurized milk (10). In order to isolate and identify the Staphylococcus aureus from these samples. The collected milk samples were cultured on nutrient agar; the presumptive Staphylococcus colonies were sub-cultured on Mannitol Salt Agar (MSA) and confirmed by using standard Bacteriological methods. Antimicrobial susceptibility pattern of Staphylococcus aureus was done by Kirby-Bauer disk diffusion method using eight antimicrobials. The prevalence of S.aureus was found to be 25 (50%) out of the total samples examined. In addition, the prevalence of S.aureus was 5 (33.3%) from Goat milk, 16 (64%) from Buffalo milk and 4 (40%) from pasteurized milk. S.aureus was more likely to occur in buffalo milk that were poorly managed and treated frequently with antimicrobials. Thus, out of a total of 25 isolates, high resistance rate was observed primarily to Methicillin 25 (100%) followed by Erythromycin15 (60%), Amoxycillin12 (48%), Vancomycin12 (48%), Gentamycin 12 (48%), Tetracycline 11 (44%), Ciprofloxacin 11 (44%), and low level of resistance to Kanamycin 8 (32%). S.aureus became almost resistant to β-lactams and Erythromycin. The virulence characters of Staphylococcus aureus were characterized phenotypically. The rate of positiveness for Protease, Lipase, β-hemolysis, β-lactamase and Slime formation were 48%, 60%, 60%, 44%, 48%.In this study we concluded that the hygiene of milk is poor and resistant strains have contaminated the milk probably during the process of milking and transportation.
Polish Journal of Veterinary Sciences, 2013
The aim of this study was to examine virulence factors and the ability of S. aureus and CNS species isolated from milk of cows with mastitis to form biofilm, and to compare them with virulence factors of staphylococci from milk of cows without mastitis and cowshed environment. Most of S. aureus strains from cows with mastitis showed haemolytic activity (93.9%), among them 72.7% and 21.2% produced α- and β-haemolysin, respectively. S. aureus from cows with mastitis symptoms produced proteases (above 48%) and esterase (42.4%). The highly significant relationship between the number of S. xylosus strains producing haemolysins (62%) and the origin of these strains from milk of cows with mastitis was observed. The ability to produce proteases was significantly associated with S. sciuri from milk of cows with mastitis. The ability of biofilm formation by staphylococcal strains from milk of cows with mastitis was greater than in strains from milk of cows without mastitis and the difference ...
International Journal of Livestock Production, 2015
The isolation, identification of Staphylococcus aureus from bovine milk and its antibiotic susceptibility was studied. One milliliter of each freshly drawn milk sample was inoculated into 9 ml of sterile peptone water and incubated overnight. A loop full of peptone water broth was streaked on blood agar base enriched with sheep blood using the quadrant streaking method for each quarter. Blood agar plates were incubated aerobically at 37°C for 24 h. S. aureus was identified by the tube coagulase test. Antimicrobial sensitivity test was conducted on S. aureus isolates using 18 antibiotics. From the study 43.75% had subclinical mastitis, while none was tested positive for clinical mastitis. None of the S. aureus isolates was fully sensitive to all the agents; 3 (42.86%) isolates were resistant to 10 agents, followed by 2 (28.57%) isolates which were resistant to 4 agents.
Brazilian Journal of Microbiology, 2000
This study reports results on the biochemical characteristics of 274 Staphylococcus aureus strains isolated from mastitic milk, hands of milkmen and milking machines. Features included colony morphology on Baird Parker agar (BPA), catalase, coagulase and thermonuclease tests. API-Staph (bioMérieux, France) was used as reference identification system. 39.4% of the colonies on BPA identified as S.aureus were atypical. They were more frequent in milk samples (94.4%). All S.aureus strains were coagulase positive but intensity of the test varied according to the source: environmental strains were 3+ or 4+, while 17.1% of strains isolated from milk presented only 1+ or 2+ results. The majority of atypical S.aureus strains presented a 4+ coagulase result. 2.2% of S.aureus strains were thermonuclease negative. Sixteen thermonuclease positive and coagulase positive strains were identified as S.hyicus.
Isolation and characterization of Staphylococcus aureus from raw cow milk in Bangladesh
The study was intended to identification and characterization of Staphylococcus aureus isolated from raw cow milk. A total of 47 milk samples were collected from Sheshmore, Shutiakhali and Bangladesh Agricultural University Dairy Farm, Mymensingh. Using bacteriological, biochemical and PCR-based identification schemes, 12 (25.53%) isolates were confirmed as S. aureus. All the isolates showed β-hemolysis on 5% sheep blood agar. S. aureus specific nuc gene (target size 279-bp) was amplified in the cases of all isolates. The isolates were found as resistant to Penicillin (100%), Erythromycin (75%) and Amoxicillin (100%). On the other hand, the isolates were sensitive to Ciprofloxacin (83.33%), Oxacillin (100%), Cloxacillin (100%) and Neomycin (100%). The isolated S. aureus showed increased resistance to broad spectrum antibiotic (e.g., Ciprofloxacin); this situation is alarming for the dairy industry and public health.
Scientific research and essays
Two hundred and fifty different samples were collected from bovine and examined for the presence of staphylococcal bacteria. 189 isolates were able to grow on the mannitol salt agar (MSA), known as staphylococci. Coagulase test revealed that 165 isolates were able to produce this enzyme; 138 of these isolates were Staphylococcus aureus which appeared in 55.2% of the isolates. Deoxyribonuclase (DNAase), urase and beta haemolysis activities of the isolates were also investigated and it showed 90.69, 86.23, and 87.86% of the isolates respectively. An enzymatic examination of the isolates was combined in numerous tests like catalase test, coagulase test, non-producing oxidase, sugar fermentation, oxidative and fermentation test, liquefaction of gelatin and MR-VP test. The polymerase chain reaction (PCR) amplification of coa gene products of S. aureus showed the following: gene product of 500 bp (22.5%); 650 bp (15%); 800 and 850 bp (25% for each); and 600 bp (12.5%).