Diaporthe diversity and pathogenicity revealed from a broad survey of grapevine diseases in Europe (original) (raw)
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Fungal biology, 2015
Trunk diseases in grapevine (Vitis spp.) are major problems in the wine and table-grape industries reducing the productivity, quality and longevity of vineyards. Species of Diaporthe are important fungal pathogens of grapevine trunk disease worldwide. A survey of 14 grape vineyards located in different provinces of China was yielded Diaporthe isolates associated with symptomatic grapevine wood. These isolates were identified based on morphology and a combined data matrix of rDNA ITS, partial sequences of translation elongation factor 1-α (EF 1-α), β-tubulin (TUB) and calmodulin (CAL) gene regions. Four species of Diaporthe were identified, which included Diaporthe eres, Diaporthe hongkongensis, Diaporthe phaseolorum and Diaporthe sojae. All isolates of Diaporthe caused disease on detached grape shoots in pathogenicity experiments but differed in virulence. The incidence in local vineyards and the pathogenicity results indicate that D. eres is an important pathogen of grapevine in Ch...
Frontiers in Microbiology
Grapevine trunk diseases have become one of the main threats to grape production worldwide, with Diaporthe species as an emerging group of pathogens in China. At present, relatively little is known about the taxonomy and genetic diversity of Chinese Diaporthe populations, including their relationships to other populations worldwide. Here, we conducted an extensive field survey in six provinces in China to identify and characterize Diaporthe species in grape vineyards. Ninety-four isolates were identified and analyzed using multi-locus phylogeny. The isolates belonged to eight species, including three novel taxa, Diaporthe guangxiensis (D. guangxiensis), Diaporthe hubeiensis (D. hubeiensis), Diaporthe viniferae (D. viniferae), and three new host records, Diaporthe gulyae (D. gulyae), Diaporthe pescicola (D. pescicola), and Diaporthe unshiuensis (D. unshiuensis). The most commonly isolated species was Diaporthe eres (D. eres). In addition, high genetic diversity was observed for D. eres in Chinese vineyards. Haplotype network analysis of D. eres isolates from China and Europe showed a close relationship between samples from the two geographical locations and evidence for recombination. In comparative pathogenicity testing, D. gulyae was the most aggressive taxon, whereas D. hubeiensis was the least aggressive. This study provides new insights into the Diaporthe species associated with grapevines in China, and our results can be used to develop effective disease management strategies.
Arhiv Za Higijenu Rada I Toksikologiju, 2012
Grapevine trunk diseases (GTD) have a variety of symptoms and causes. The latter include fungal species from the family Diaporthaceae. The aim of our study was to determine Diaporthaceae species present in the woody parts of grapevines sampled from 12 vine-growing coastal and continental areas of Croatia. The fungi were isolated from diseased wood, and cultures analysed for phenotype (morphology and pathogenicity) and DNA sequence (ITS1, 5.8S, ITS2). Most isolates were identifi ed as Phomopsis viticola, followed by Diaporthe neotheicola and Diaporthe eres. This is the fi rst report of Diaporthe eres as a pathogen on grapevine in the world, while for Diaporthe neotheicola this is the fi rst report in Croatia. Pathogenicity trials confi rmed Phomopsis viticola as a strong and Diaporthe neotheicola as a weak pathogen. Diaporthe eres turned out to be a moderate pathogen, which implies that the species could have a more important role in the aetiology of GTD.
Plant Disease, 2013
Baumgartner, K., Fujiyoshi, P. T., Travadon, R., Castlebury, L. A., Wilcox, W. F., and Rolshausen, P. E. 2013. Characterization of species of Diaporthe from wood cankers of grape in eastern North American vineyards. Plant Dis. 97:912-920.
Journal of Fungi
Grapevine trunk diseases (GTD) are currently one of the most devastating and challenging diseases in viticulture, leading to considerable yield losses and a remarkable decline in grapevine quality. The identification of the causal agents is the cornerstone of an efficient approach to fighting against fungal diseases in a sustainable, non-chemical manner. This review attempts to describe and expose the symptoms of each pathology related to GTD, the modes of transmission, and the harmfulness of recently reported agents. Special attention was given to new diagnostic tests and technologies, grapevine defense mechanisms, molecular mechanisms of endophytes fungal colonization, and management strategies used to control these threats. The present extended review is, therefore, an updated state-of-the-art report on the progress in the management of vineyards.
Species of Diatrypaceae associated with grapevine trunk diseases in Eastern Spain
The presence and diversity of Diatrypaceae species occurring on grapevines in Eastern Spain were investigated. Several species were identified on the basis of morphological characters and phylogenetic analyses of the complete sequence of the internal transcribed spacers of the ribosomal DNA and part of the β-tubulin gene. Five species of Diatrypaceae isolated from the wood of diseased grapevines, pruning debris and/or perithecia were identified, including Anthostoma decipiens, Cryptovalsa ampelina, Eutypa lata, Eutypella citricola and Eutypella microtheca. Additionally, four taxa could not be identified to the species level but were closely related to Eutypa tetragona based on phylogenetic analyses. Eutypa lata was the most prevalent species and showed the greatest degree of genetic diversity. Cryptovalsa ampelina and E. microtheca ranked second in the frequency of isolations, while all the remaining species were less frequently isolated. Eutypella citricola and E. microtheca are reported for the first time as occurring on grapevine in Spain and this is the first report of A. decipiens occurring on grapevine.
Editorial: Fungal pathogens causing the grapevine trunk diseases- biology and identification
Frontiers in fungal biology, 2023
Editorial on the Research Topic Fungal pathogens causing the grapevine trunk diseases-biology and identification Grapevine (Vitis vinifera L.) is widely cultivated as an important fruit crop destinated for wines, table grapes, and raisins. However, grapevines are plagued by a variety of diseases each year, depending on the causal agents, cultivar susceptibility, and climate location, and these diseases are limiting factors in commercial vineyards and nurseries (Wilcox et al., 2015; Muntean et al., 2022). In this sense, Grapevine Trunk Diseases (GTDs) are one the most critical factors affecting vineyards' productivity and quality (Wilcox et al., 2015;
The Spread of Grapevine Trunk Disease
Science, 2009
This review presents an overview of eutypa dieback, esca and botryosphaeria dieback, the predominant grapevine trunk diseases worldwide. It covers their symptomatologies in the trunk, leaves and berries; the characteristics of the different fungal species associated with them; and host-pathogen interactions. Here, the host-pathogen relationship is defined at the cytological, physiological and molecular levels. Currently available experimental tools for studying these diseases, both in vitro and in the field, are discussed. Finally, a progress report on their control, which, since the ban of sodium arsenite, comprises chemical, biological and ⁄ or sanitation methods, is presented.
REVIEW OF VARIOUS GRAPE DISEASES
Grapevine is a foremost vegetative disseminated berry with high global socioeconomic prominence. It is vulnerable to numerous graft-transmitted agents that cause several diseases and extensive crop fatalities, decreasing fruit quality, plant strength, and also reduce the durability of vines. The vegetative transmission and frequent exchanges of propagative material within countries contribute to unfold these pathogens, esteeming the development of multifaceted diseases. Its lasting life cycle further accelerates the blending and introduction of numerous epidemiologic agents into a single plant. At present, almost 65 viruses belonging to different families have been reported infecting grapevines, however, all do not cause economically significant diseases. Regrettably, there are several breeds of vine diseases that prosper in all circumstances. Bacteria and fungi are a source of most common grapevine diseases. Insects can also propagate viruses and harm roots. Ecological circumstances can cause mold development that inflicts disorder on grapevines in vineyards. Here, we review the recent knowledge of these viruses, report advances in their diagnosis and prospection of new species, and give indications about the management of the associated grapevine diseases.
Grapevine trunk diseases: complex and still poorly understood
Plant Pathology, 2012
This review presents an overview of eutypa dieback, esca and botryosphaeria dieback, the predominant grapevine trunk diseases worldwide. It covers their symptomatologies in the trunk, leaves and berries; the characteristics of the different fungal species associated with them; and host-pathogen interactions. Here, the host-pathogen relationship is defined at the cytological, physiological and molecular levels. Currently available experimental tools for studying these diseases, both in vitro and in the field, are discussed. Finally, a progress report on their control, which, since the ban of sodium arsenite, comprises chemical, biological and ⁄ or sanitation methods, is presented.