High Throughput Profiling of Flavonoid Abundance in Agave lechuguilla Residue-Valorizing under Explored Mexican Plant (original) (raw)
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Molecules, 2021
Agave lechuguilla agro-waste is a promising renewable material for biorefining purposes. The procurement of added-value co-products, such as bioactive phytochemicals, is required to improve bioprocesses and promote the bio-based economy of the productive areas of Mexico. In this study, we aimed to evaluate the effect of post-harvest management and enzymatic pretreatment as the first stages of the A. lechuguilla valorization process. Four drying methods were compared, and enzymatic hydrolysis was optimized to obtain a flavonoid-enriched extract applying ultrasound-assisted extraction. In both experiments, the total phenolic (TPC) and flavonoid (TFC) contents, HPLC-UV flavonoid profiles, and radical scavenging capacity (DPPH) were considered as response variables. The results demonstrated that light exposure during the drying process particularly affected the flavonoid content, whereas oven-dehydration at 40 °C in the dark preserved the flavonoid diversity and antioxidant functionalit...
Background: Many plants, particularly medicinal plants, have been extensively studied for their antioxidant activity in recent years. americanais one of the 500 more widely used medicinal plants in several countries. antioxidant capacity, total phenolic content and total flavonoids content of four different fractions (ethyl acetate, chlorof aqueous fraction). An experimental study was realized on the test. The total phenolic contents (TPC) and the total flavonoids contents (TFC) of the four different fractions were measured ciocalteu assay and aluminum trichloride 172.92±14.30 mg/100g of gallic acid equivalent of fraction. The total flavonoids content varied from 1.24 catechin equivalent. Conclusion : All extracts showed a good antioxidant capacity and ethyl acetate fraction from Kristel area showed the highest antioxidant capacity with a value of IC
Journal of Agricultural and Food Chemistry, 2007
A screening method was developed for the systematic identification of glycosylated flavonoids and other phenolic compounds in plant food materials based on an initial, standard analytical method. This approach applies the same analytical scheme (aqueous methanol extraction, reverse phase liquid chromatographic separation, and diode array and mass spectrometric detection) to every sample and standard. This standard approach allows the cross-comparison of compounds in samples, standards, and plant materials previously identified in the published literature. Thus, every analysis contributes to a growing library of data for retention times and UV/vis and mass spectra. Without authentic standards, this method provides provisional identification of the phenolic compounds: identification of flavonoid backbones, phenolic acids, saccharides, and acyls but not the positions of the linkages between these subclasses. With standards, this method provides positive identification of the full compound: identification of subclasses and linkages. The utility of the screening method is demonstrated in this study by the identification of 78 phenolic compounds in cranberry, elder flower, Fuji apple peel, navel orange peel, and soybean seed
Journal of Chromatography A, 2006
A high-performance liquid chromatography (HPLC) method with photo-diode array (DAD) detection was developed to separate and quantify flavonoids in sugarcane leaves and bagasse (= the crushed sugarcane refuse from juice extraction), and in sugarcane juice. Sugarcane flavonoids consist of a complex mixture of aglycones and glycosides (including flavonolignan glycosides), and the HPLC-UV method herein proposed is suitable for their quantification as total flavonoids. This method was applied to analyze samples of cultivated sugarcane, commercial juice and transgenic sugarcane leaves. Sugarcane leaves proved a promising source of flavonoids: an average of 1.10 mg of total flavonoids/g plant material was found in fresh leaves. Moreover, the flavonoid content of sugarcane juice (0.6 mg/mL) is comparable to other food sources of flavonoids previously reported. Transgenic sugarcane leaves ("Bowman-Birk" and "Kunitz") were compared with non-modified ("control") plant samples using the proposed HPLC-UV method, which indicated that the content of total flavonoids in transgenic plants is different from that in non-modified sugarcane.
Journal of Chromatography A, 2004
Steam distillation (SD) extraction-solid-phase microextraction coupled to GC-MS was developed for the determination of terpenes and Bligh-Dyer extraction-derivatization coupled with GC for the determination of fatty acids such as ethyl esters were used. It was found that the three different Agave species have the same profile of fatty acids; the quantity of these compounds is different in each Agave variety. On the other hand, different terpenes were identified in the three Agave plants studied: nine in A. salmiana, eight in A. angustifolia and 32 in A. tequilana Weber var. azul.
Data in Brief, 2019
A comparative data of two generally accepted mass techniques for estimate the molecular weight distribution of polysaccharides are presented. The data were obtained from agave fructans samples of different molecular weight, which were analyzed authored independently. The two analytical techniques used were Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-ToF) and High Pressure Size Exclusion Chromatography (HP-SEC). The data set here are related to the research paper entitled "Size-exclusion chromatography (HPLC-SEC) technique optimization by simplex method to estimate molecular weight distribution of agave fructans" by Moreno-Vilet et al. [1]. This article present the comparative figures as histograms obtained from mathematically processed MALDI-ToF spectra and HPLC-SEC chromatograms. And also, the calculated polymer parameters as number and mass molecular weight, number and mass average degree of polymerization and dispersity index.
Phytochemical profiles and classification of Agave syrups using 1 H-NMR and chemometrics
Food Science & Nutrition
Agave syrups are natural sweet substances produced when Agave pines are subjected to a fructan hydrolitic process in order to unfold polysaccharides called agavins (NMX-FF-110-SCFI, 2008) Agavins are fructose polymers where the number of molecules plays an inverse relationship with sweetness: the lower the sweetness, the longer the degree of polymerization. To produce Agave syrups, plants must be at least 6 years old corresponding to their proximate maturity, as well as their maximum carbohydrates content (Mellado-Mojica & López, 2013). In the Agave syrups production, the principal agronomic species used are Agave tequilana Weber Blue variety and Agave salmiana displaying differences in carbohydrate content and composition (Bautista,
Total flavonoids quantification from O/W emulsion with extract of Brazilian plants
International Journal of Pharmaceutics, 2006
A new derivative spectrophotometric (DS) method was proposed and validated for quantification of total flavonoids from in O/W emulsion with polyacrylamide (and) C13-14 isoparaffin (and) laureth-7 containing Catuaba (Trichilia catigua Adr. Juss) (and) Marapuama (Ptychopetalum olacoides Bentham) extract. DS method was optimized to perform the assay in most favorable conditions. Linearity, specificity and selectivity, recovery (Rc, %), precision (R.S.D., %), accuracy (E, %), detection (LOD, g ml −1 ) and quantification limits (LOQ, g ml −1 ) were established for method validation. First-derivative at 388.0 nm (zero-to-peak; amplitude = ± 0.12; wavelength range = 300.0-450.0 nm and λ = 4 nm) offered linearity for rutin concentrations ranging from 10.0 to 60.0 g ml −1 in ethanol 99.5%. Second-derivative provided to be unsuitable for interval evaluation obtaining unacceptable accuracy. Analytical method was validated for first-derivative, according to the experimental results: correlation coefficient (r = 0.9999); specificity to total flavonoids quantification, expressed in rutin, at wavelength 388.0 nm and selectivity with elimination of interference from matrix; Rc = 108.78%; intra-and inter-run precision (1.30-3.65% and 3.48-4.68%), and intra-and inter-run accuracy (100.00-112.19% and 101.25-118.44%); LOD = 0.62 g ml −1 and LOQ = 1.86 g ml −1 .
Problem statement: In Durango, Mexico, mescal is elaborated from wild plants of Agave durangensis. This species shows a high morphological variability within and among populations, what makes its taxonomic delimitation a hard task. Approach: In this study the pollen and foliar phenolic compositions of Agave durangensis were analyzed by HPLC/DAD with the aim of determining the significance of phenol profiles to delimit this taxon. Results: The foliar phenol compositions were evaluated within and among two populations and between juvenile and adult plants. Agave asperrima Jacobi, Dasylirion sp. and juvenile samples of A. shrevei Gentry subsp. shrevei, A. shrevei Gentry subsp. matapensis Gentry and A. wocomahi Gentry, were also analyzed to stand comparisons with. The results from this study indicated that pollen and foliar tissues of Agave durangneisis were rich in kaempferol glycoside derivatives (13 and 23 different compounds can be present, respectively). Principal coordinates analysis (PCO), based on foliar profiles of adults, indicated the presence of several chemotypes within the Type locality of Agave durangensis and revealed chemical differences between the both analyzed populations. Conclusion/Recommendations: Chemical and morphological differences and biogeographical evidence suggest the recognition of two different taxonomic entities in this morphological variable group.