Comparison of Hyssopus officinalis, Tussilage farfara, Carum copticum extracts versus systemic glucantime in the treatment of cutaneous leishmaniasis in Balb/c mice (original) (raw)
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Advanced studies in biology, 2014
Leishmania is transmitted by sandflies that ingest the parasite in the amastigote phase resident within macrophages, then inoculate the promastigote phase into body hosts. The present study was conducted to evaluate the in vitro effects of alcoholic extract of Plants on L.major. The extract of aerial parts of plants were obtained by maceration. The in vitro experiments were performed on promastigotes to assess antileishmanial activity of the extracts using glucantime as 194 Fatemeh Tabatabaie et al. a reference. The extract of plants and glucantime solutions for biological testing were prepared in PBS at 0.05-0.1-0.2-0.4 μg/mL and 1μg/mL, respectively. All experiments were repeated at least three times in duplicate. For the extract of plants and glucantime, the concentration-response curve was plotted, from which IC50 values were determined also No and MTT assay were done. The different concentrations resulted in different optical densities or inhibitory percentages (P<0.05) so that extract of plants were effective against L. major in vitro. NO production the extract of plants showed significant in vitro antileishmanial activities.The Findings of this study indicate that these plants are effective against L.major in vitro.
Leishmania is transmitted by sandflies that ingest the parasite in the amastigote phase resident within macrophages, then inoculate the promastigote phase into body hosts. The present study was conducted to evaluate the in vitro effects of alcoholic extracts of plants on L. major. The extract of aerial parts of plants were obtained by maceration. The in vitro experiments were performed on promastigotes to assess anti Leishmanial activity of the extracts using glucantime as a reference. The extract of plants and glucantime solutions for biological testing were prepared in PBS atseveral concentration, respectively. All experiments were repeated at least three times in duplicate. For the extract of plants and glucantime, the concentration-response curve was plotted, from which IC50 values were determined also MTT assay was done. The different concentrations resulted in different optical densities or inhibitory percentages (P<0.05) so that extract of plants were effective against L. ...
In vitro and in vivo susceptibility of Leishmania major to some medicinal plants
Objective: To evaluate the efficacy of some medicinal plants and systemic glucantime in a comparative manner against the causative agent of cutaneous leishmaniasis both in vitro and in BALB/c mice. Methods: For in vivo testing, inbred mice were challenged with Leishmania major parasites and the resultant ulcers were treated with extract based-ointments applied topically two times per day for a period of 20 days. A group of 56 mice were randomly divided into 7 subgroups. The control group received the ointment void of extracts, whereas the reference group received glucantime only. The efficacy of treatments was evaluated by measuring ulcer diameter, parasite burden and NO production. Results: Our results indicated that plant extract based-ointments were effective in reducing ulcer size and parasite burden in spleens, but their effects did not differ significantly from that of glucantime. The plant extracts tested in this study were able to increase NO production that helped parasite suppression. Conclusions: Our findings indicate that the tested plant extracts are effective against Leishmania major both during in vitro and in vivo experiments, but further researches are required to recommend a potential plant extract as an alternative drug.
In Vitro Activity of Hyssopus Officinalis, Tussilage Farfara, Carum Copticum Extracts Against
2015
Asadi, Mehri Noori and Fatemeh Maleki. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Leishmania is transmitted by sandflies that ingest the parasite in the amastigote phase resident within macrophages, then inoculate the promastigote phase into body hosts. The present study was conducted to evaluate the in vitro effects of alcoholic extract of Plants on L.major. The extract of aerial parts of plants were obtained by maceration. The in vitro experiments were performed on promastigotes to assess antileishmanial activity of the extracts using glucantime as 194 Fatemeh Tabatabaie et al. a reference. The extract of plants and glucantime solutions for biological testing were prepared in PBS at 0.05-0.1-0.2-0.4 μg/mL and 1μg/mL, respectively. All experiments were repeated at least three times in duplicate. For the extract o...
BMC Research Notes, 2021
ObjectivesThe current study aimed to evaluate the effects ofFicus caricalatex on the treatment of cutaneous leishmaniasis (CL), induced byLeishmania major.A 5% topical gel withF. caricalatex was prepared. BALB/c mice were infected by inoculation of amastigotes form ofL. major.Thirty BALB/c mice were divided into five groups, where the first group was treated daily, the second group twice per day, and the third group every other day with the 5% topical gel, for 3 weeks. The sizes of the lesions were measured before and during the course of treatment.ResultsAlthough the mean size of lesions in the mice group treated with the 5%F. caricagel, especially in the group receiving daily treatment, was less than the mean size of the lesions in the control group, yet, the differences was not statistically significant (p > 0.05). The findings of the current study demonstrated that the 5%F. caricalatex with a 3-week course of treatment had no considerable effect in recovery or control of CL i...
Anti-leishmanial and toxicity activities of some selected Iranian medicinal plants
Parasitology Research, 2012
Leishmaniasis is caused by protozoan parasites belonging to the genus Leishmania. Cutaneous leishmaniasis is the most common form of leishmaniasis in Iran. As there is not any vaccine for leishmaniasis, treatment is important to prevent the spreading of parasites. There is, therefore, a need to develop newer drugs from different sources. The aim of this study was to assess anti-leishmanial activity of the ethanolic extracts of 17 different medicinal plants against Leishmania major promastigotes and macrophage cell line J774. The selection of the hereby studied 17 plants was based on the existing information on their local ethnobotanic history. Plants were dried, powdered, and macerated in a hydroalcoholic solution. Resulting extracts have been assessed for in vitro anti-leishmanial and brine shrimp toxicity activities. Four plants, Caesalpinia gilliesii, Satureia hortensis, Carum copticum heirm, and Thymus migricus, displayed high antileishmanial activity (IC50, 9.76±1.27, 15.625±3.76, 15.625 ±5.46, and 31.25±15.44 μM, respectively) and were toxic against the J774 macrophage cell line at higher concentrations than those needed to inhibit the parasite cell growth (IC50, 45.13 ± 3.17, 100.44 ± 17.48, 43.76 ± 0.78, and 39.67 ± 3.29 μM, respectively). Glucantime as positive control inhibited the growth of L. major promastigotes with IC500 254 μg/ml on promastigotes (1×10 6 /100 μ/well) of a log phase culture, without affecting the growth of J774 macrophages. These data revealed that C. gilliesii, S. hortensis, C. copticum heirm, and T. migricus extracts contain active compounds, which could serve as alternative agents in the control of cutaneous leishmaniasis. The activity of these herbs against L. major promastigotes and macrophage cell line J774 was reported for the first time in our study.
The Most Important Native Medicinal Plants Effective Against Cutaneous Leishmaniasis in Mouse
2018
Leishmaniasis is a zoonotic parasitic disease that is caused by different Leishmania species. Most cases of leishmaniasis are reported from Afghanistan, Saudi Arabia, Algeria, Brazil, Iraq, and Iran. Antimony compounds have long been used as standard treatment and first line drugs for leishmaniasis, but Leishmania species have recently acquired drug resistance. Nowadays, medicinal plants are being increasingly used to treat parasitic diseases especially leishmaniasis. In this review, the search terms Leishmania, leishmaniasis, mouse, Iran, and medicinal plants were used to retrieve publications from databases such as Scopus, Islamic World Science Citation Center, Scientific Information Database, and Magiran. According to the results of this review, nine medicinal plants, Eucalyptus camaldulensis, Matricaria chamomilla, Cathrantus roseus, Echinacea purpurea, Lawsonia inermis, Artemisia sieberi, Berberis vulgaris, Allium sativum L., and Lavandula spica L. have been reported to be effe...
2011
Cutaneous leishmaniasis is the most common form of leishmaniasis that is still treated with expensive drugs, which present side effects. Conventional healers usually are cheap and sometimes more effective than chemical drugs. Satureja khuzestanica is an endemic plant in the west of Lorestan Province, Iran. This plant has therapeutic value. This study was carried out for the first time to assess the in vivo efficacy of S. khuzestanica in animal model of leishmaniasis. Mice were inoculated with Leishmania major poromastigotes and then when a lesion developed, the mice were divided randomly into treatment and control groups. The mice were treated with different concentrations of S. khuzestanica essential oil (SKEO). The results showed that the lesions' size in SKEO treated groups was restrained but not significantly different from the control group which might be due to the low sample size or concentration of SKEO. The mortality rate in treated groups was clearly less than the control. The results indicated that SKEO has an effect on preventing death in infected mice. Also the survival rate in the treated groups compared with the control groups was more. According to the results, it seems that not only SKEO is antifungal, antiviral, antibacterial but also antiparasitical.
International journal of medical laboratory, 2019
Background and Aims: Because of the toxicity and side-effects of synthetic drugs, there is a growing interest in biomedical plants. The aim of this study was to evaluate in vitro antileishmanial activity of Carum copticum essential oil against Leishmania (L) major. Materials and Methods: Nineteen experimental groups were designed to determine the effect of Carum copticum essential oil against L. major and compare it with Meglumine antimonite. Group 1 was the control group and included 200 µl of RPMI 1640 plus 2×10 5 cells/ml promastigotes. Groups 2-10 included the aforementioned substances plus 10 µl of 0.01, 0.02, 0.05, 0.1, 0.2, 0.5, 1, 2 and 3 µg/ml of Carum copticum essential oil respectively. Groups 11-19 were similar to groups 2-10 but Meglumine antimonite (0.01, 0.02, 0.05, 0.1, 0.2, 0.5, 1, 2 and 3 µg/ml) was used instead of Carum copticum essential oil. All the experiments were repeated five times. After 8 hours, the antileishmanial activities of studied substances were determined. Results: Up to concentration of 0.5 µg/ml, no effect was observed with both substances. In comparison to control group, at 1 and 2 µg/ml, Meglumine antimonite had no effect on Leishmaniasis (p>0.05) while Carum copticum essential oil significantly decreased Leishmaniasis viability (p<0.05). Moreover, at 3 µg/ml, both compounds significantly decreased Leishmaniasis viability (p<0.05). However, Carum copticum essential oil had substantially better Antileishmanial activity than the other. Conclusions: These results suggest that comparable concentrations, in vitro antileishmanial activity of Carum copticum essential oil is better than Meglumine antimonite.
Journal of Parasitic Diseases, 2016
The role of immunomodulation in the therapeutic treatment of visceral leishmaniasis has gained eminence in view of moderate to severe drawbacks of the currently available drugs like toxicity, drug resistance and prohibitive costs. The potential for modulation of the immune system of many herbal plants can be tapped to address these problems. We conducted the present research study to investigate the antileishmanial and immunomodulatory effects of Ocimum sanctum Linn. and Cocos nucifera Linn. during the progression of visceral leishmaniasis in BALB/c mouse model. The IC 50 values of the ethanolic leaf extract of O. sanctum and that of the aqueous husk-fibre extract of C. nucifera against the sodium stibogluconate (SSG) susceptible strain (MHOM/IN/80/Dd8) were found to be 73.3 and 62 lg/ml respectively. On treatment of infected BALB/c mice with the extracts, we observed a reduction in hepatic parasite load by 43.63 % (O. sanctum), 65.42 % (C. nucifera) and 75.61 % (O. sanctum ? C. nucifera) at 1st post treatment day (p.t.d.), while at 15th p.t.d., the reduction was 73.61 % (O. sanctum), 76.59 % (C. nucifera) and 94.12 % (O. sanctum ? C. nucifera). This was accompanied by an upscaling of the DTH response, skewing of the humoral response towards Th1 type and hepatoprotection in the form of normalization of liver function tests. Overall, administration of the extracts of these two plants in combination as compared to their administration alone rescued the affected mice from the disease greatly, which can be attributed to their antileishmanial and immunomodulatory activities.