Protective Effect of Vitamins C and E on Depot-Medroxyprogesterone Acetate-Induced Ovarian Oxidative Stress In Vivo (original) (raw)

Protective effect of combined vitamin C and E against ovarian and endometrial toxicity in rats that receiving oral rhodamine B

Biomarkers and Genomic Medicine, 2015

This study aimed to investigate whether combined supplementation with vitamin C and vitamin E was able to modify the superoxide dismutase (SOD) and malondialdehyde (MDA) levels in the ovarium of rats exposed to rhodamine B. Twenty-five female Wistar albino rats were divided into five groups (n = 5 each), including control (untreated group); rhodamine B group; rhodamine B group which received vitamin C (0.2 mg) + vitamin E (0.04 IU/g body weight); rhodamine B group which received vitamin C (0.4 mg) + vitamin E (0.04 IU/g body weight); and the rhodamine B group which received vitamin C (0.8 mg) + vitamin E (0.04 IU/g body weight). Analysis of MDA levels as a marker of lipid peroxidation was done spectrophotometrically. Analysis of SOD levels was done by enzyme-linked immunosorbent assay technically. Endometrial histology was analyzed in hematoxylin eosin staining. This increase in ovarian MDA was significantly (p 0.05). All doses also significantly prevented rhodamine B-induced decr...

Ovarian toxicity in rats caused by methidathion and ameliorating effect of vitamins E and C

Human & Experimental Toxicology, 2007

Experimental groups were as follows: control group; a group treated with 5 mg/kg body weight MD (MD group); and a group treated with 5 mg/kg body weight MD plus vitamin E and vitamin C (MD ϩ Vit group). MD and MD ϩ Vit groups were given MD by gavage five days a week for four weeks at a dose level of 5 mg/kg/day by using corn oil as the vehicle. Serum malondialdehyde (MDA: an indicator of lipid peroxidation) concentration, serum activity of cholinesterase (ChE), and ovary histopathology were studied. The level of MDA increased significantly in the MD group compared with the control (P Ͻ 0.005). Serum MDA decreased significantly in the MD ϩ Vit group compared with the MD group (P Ͻ 0.05). The activities of ChE decreased significantly both in the MD and MD ϩ Vit groups compared with the controls (P Ͻ 0.05). However, the decrease in the MD ϩ Vit groups was less than in the MD group; the ChE activity in the MD ϩ Vit group was significantly higher compared with MD group (P Ͻ 0.05). Number of ovarian follicles were significantly lower in the MD group compared to the controls (P Ͻ 0.05). Number of atretic follicles were significantly higher in the MD group than in the controls (P Ͻ 0.05). Follicle counts in MD ϩ Vit group showed that all types of ovarian follicles were significantly higher, and a significant decrease in the number of atretic follicles compared with the MD group (P Ͻ 0.05). In conclusion, subchronic MD administration caused an ovarian damage, in addition, LPO may be one of the molecular mechanisms involved in MD-induced toxicity. Treatment with vitamins E and C after the administration of MD reduced LPO and ovarian damage. Human & Experimental Toxicology (2007) 26, [491][492][493][494][495][496][497][498]

Changes in the oxidant/antioxidant balance in rats with pharmacologically induced ovarian failure

2018

Reactive oxygen species are constantly produced in all aerobic organisms, following intracellular metabolism, in response to environmental stimuli. With a composition including free oxygen radicals, they are incriminated in inflammatory, cardiovascular diseases, playing a role in the damage and loss of function of various tissues and organs. The aim of the present work was to assess the effect of different cyclophosphamide doses onto the serum oxidant/antioxidant balance in female rats with ovarian failure. We also monitored the effect after the administration of an antioxidant complex (Sel-E-Vit). Oxidative stress was measured based on the serum levels of malondialdehyde and protein carbonyls; antioxidant defence was quantified based on the serum levels of hydrogen donors and reduced glutathione. Sel-E-Vit treatment causes changes in the oxidant/antioxidant balance of animals with cyclophosphamide-induced ovarian failure. Our results demonstrate the presence of oxidative stress in ...

Vitamin C suppresses ovarian pathophysiology in experimental polycystic ovarian syndrome

Pathophysiology, 2019

Background: Polycystic ovary syndrome (PCOS), also known as the Stein-Leventhal syndrome is one of the most common causes of anovulation, infertility and hyperandrogenism in women, affecting between 5-10 % of women of reproductive age (12-35 years) worldwide. Despite substantial effort to define the cause of PCOS, its pathophysiology remains poorly understood. Consequently, determining the mechanisms of PCOS and the possible treatment is the major goal of medical research in endocrine and reproductive physiology. Aim: To investigate the mechanism of ovarian metabolic changes in dehydroepiandrosterone (DHEA)induced polycystic ovary in Wistar rats treated with vitamin C. Methods: Twenty-eight immature female Wistar rats weighing (16-21 g) were randomly divided into four groups (n = 7/group): group I served as control and was given water, group II were injected with DHEA (6 mg/100 g in 0.2 ml corn oil subcutaneously to induce PCOS condition), group III received 150 mg/kg BW of Vitamin C orally, group IV were co-administered with 6 mg/kg BW DHEA in 0.2 ml of corn oil subcutaneously and 150 mg/kg BW of Vitamin C orally. All treatments lasted for 15 days. Twenty-four hours after the last administration, the rats were sacrificed by cervical dislocation. Blood samples and ovaries were collected for reproductive hormonal analysis, biochemical and histopathological analysis. The expressions of mRNA androgen receptor gene in the ovary were determined by real-time reverse transcriptase polymerase chain reaction. All data were analysed using one-way ANOVA. Results: There was a significant decrease (p < 0.05) in the antioxidant and metabolic enzyme activity in the DHEA treated group compared with the control group. DHEA co-administration with Vitamin C showed a significant decrease in Malondialdehyde, cytokines and Estrogen and a significant increase (p < 0.05) in antioxidant and metabolic enzymes compared with DHEA treated group only. The histopathological evaluation demonstrates a reduction in cystic and atretic ovaries, increased expression of Bcl 2 and E-Cadherin with a reduction in Bax expression in the group co-administered with DHEA and Vitamin C. The DHEA group showed overexpression of mRNA Androgen Receptor gene in the ovaries compared to the control group. Conclusion: This study shows that Vitamin C plays a protective role against DHEA-Induced Polycystic Ovary in Wistar rats via its antioxidant and anti-apoptotic mechanisms.

Study of oxidative stress and antioxidant levels in polycystic ovarian disease

2015

Background: Polycystic ovary disease (PCOD) is a common but complex endocrine disorder and is a major cause of anovulation and consequent infertility. The stressful lifestyle is leading to increased prevalence of polycystic ovarian disease in young adolescent and early reproductive population and its association with many ongoing complications such as infertility, obesity, insulin resistance, dyslipidemia, endothelial dysfunction and overt diabetes mellitus. The study was undertaken to analyze the oxidative stress in females having polycystic ovarian disease and compare them with age and gender matched healthy subjects. We correlated oxidative stress by measuring serum Malonyldialdehyde-(MDA) levels and antioxidant levels by measuring serum Vitamin C levels in PCOD patients. Study methodology: Oxidative stress were measured in 60 Subjects within the age group of 20 to 30 years were selected and divided into two groups. Group I consisted of 30 diagnosed female patients of PCOD and Gr...

Hydrogen peroxide formation and lipid peroxidation in rat uterus—effect of hormones and vitamin E

Journal of Biosciences, 1986

The effect of estradiol-17ß and progesterone given separately as well as in combination on the rate of hydrogen peroxide formation and lipid peroxidation in the uteri of ovariectomized rats was studied. Estradiol in 3 µg dose per day per animal elicited maximum stimulatory response and progesterone (100 µg), on the other hand, was without any such effect. However, progesterone given along with estradiol completely prevented the effect due to the latter. In the same way, vitamin E, a well known antioxidant was found to be extremelv effective in protecting the uterus from the highly peroxidative action of estradiol-17ß.

The effects of acrylamide and vitamin E administration during pregnancy on adults' ovarian tissue: An experimental study

Annals of Medical Research

The aim of the present study is to investigate the effects on ovarian tissues of adult female rats, which were the offspring of rats administered acrylamide (AA) and vitamin E during pregnancy. Material and Methods: Thirty rats were divided into 5 groups of 6 pregnant rats, as the Control, Corn Oil, Vitamin E, AA, Vitamin E + AA groups. The births were monitored on the 21 st day to select the female rats. The selected female rats were decapitated at the end of the 8 th week and their ovarian tissues were removed under anesthesia malondialdehyde (MDA), reduced glutathione (GSH), total antioxidant capacity (TAS), total oxidant capacity (TOS), oxidative stress index (OSI), superoxide dismutase (SOD), catalase (CAT) and nitric oxide (NO) levels were examined. Results: Administration of AA during pregnancy caused an increase in MDA, TOS, OSI and NO levels and a decrease in GSH, SOD, CAT and TAS levels in the ovarian tissues of the rats when compared to the control group. It was determined that vitamin E administration caused an increase in GSH, SOD, CAT and TAS levels in ovarian tissues, compared to all other groups (P <0.05). Conclusion: Exposure to food-induced AA toxicity increases each day and the parallel increase in infertility suggests that it could be related to AA toxicity. Although vitamin E is capable to exert a protective effect against AA toxicity through increasing the antioxidant capacity of ovarian tissue, there is certain necessity for further studies.

Oxidant/antioxidant status, paraoxonase activity, and lipid profile in plasma of ovariectomized rats under the influence of estrogen, estrogen combined with progesterone, and genistein

Drug Design, Development and Therapy, 2015

The aim of this study was to investigate whether estradiol (E2), E2 combined with progesterone (Prog) (E2/Prog), and genistein (Gen) treatment had antioxidative and antihyperlipidemic effects in the plasma of ovariectomized (OVX) rats. Materials and methods: Adult female Sprague-Dawley rats were divided into five groups. Rats in all groups, except for those in a sham group, underwent bilateral ovariectomy under general anesthesia. The groups were as follows: sham group; control OVX group; group treated with estrogen (0.014 mg/kg 17-β E2); group treated with a combination of E2 and Prog (0.014 mg/kg 17-β E2 plus 0.028 mg/kg drospirenone), and group treated with Gen (10 mg/ kg/day). Plasma of rats of each treatment group was analyzed to determine the total antioxidant status, total oxidant status, paraoxonase activity, lipid profile, high-density lipoprotein (HDL-chol), low-density lipoprotein (LDL-chol), total cholesterol (Total-C), triacylglycerols, lipoprotein (a), and oxidative stress index. Results: Plasma Total-C levels and body weight increased in all the OVX groups compared with the sham group (P0.005). The group treated with E2 had significantly elevated total oxidant status, oxidative stress index, LDL-chol, and Total-C compared with the control group (P0.005). Gen treatment might lead to lower LDL-chol and Total-C levels compared with E2 treatment. Conclusions: Gen treatment might be preferred to E2 treatment for treatment of menopausal symptoms in patients at risk for cardiovascular diseases. However, considering the small sample size of this study, larger studies are needed in this area.

Cukurova Medical Journal Vitamin C-E and Monosodium Glutamate-Induced Ovarian Toxicity Vitamin C-E ve Monosodyum Glutamata Bağlı Ovaryan Toksisite

Our study aimed to investigate the combinatorial effect of vitamin C and E on oxidative stress, the number of folicles, and hormonal level in female rats orally exposed to monosodium glutamate (MSG). Materials and Methods:Female, twelve weeks old Wistar rats ingested with of MSG at dose 140 mg/200 gram body weight (bw) with or without combination of vitamin C and E. Twenty five rats were divided into five group (n = 5 each), control group, MSG-treatment group, MSG-treatment + 0.2 mg/g bw vitamin C + 0.04 IU/g bw vitamin E (MSG + CE1), MSG-treatment + 0.4 mg/g bw vitamin C + 0.04 IU/g bw vitamin E (MSG + CE2), and MSG-treatment + 0.6 mg/g bw vitamin C + 0.04 IU/g bw vitamin E (MSG + CE1). Analysis of malondialdehyde (MDA) level was done colorimetrically. Analysis of the number of ovarian follicles was done histopathologically with hematoxylin eosin staining. Analysis of 17β-estradiol and follicle stimulating hormone (FSH) levels were done by ELISA technique. Results: There were significantly (P < 0.05) increased ovarium MDA levels and atresia follicle number in groups exposed to MSG compared to non-exposure group. The level of FSH, 17β-estradiol, the number of primary, secondary, de Graaf follicles were significantly lower in MSG-treatment group compared to control group (P < 0.05). The administration of combined vitamin C and E (second and third dose) significantly (P < 0.05) decreased the MDA levels and the number of atresia follicle compared to the MSG-exposed groups, to reach level in control group (P > 0.05). MSG + CE2 and MSG + CE3 significantly increased FSH level, number of primary follicles, compared to MSG-treatment group (P < 0.05), to reach similar level in control group (P > 0.05). All dose of combined vitamin C and E significantly increased 17β-estradiol level and the number of secondary and de Graaf follicles compared to MSG-treatment group (P < 0.05), to reach significantly higher level compared to control group (P < 0.05). Conclusion: The present data suggest that combination of vitamin C and E as in this study inhibited ovarium toxicity caused by MSG treatment.

Evaluation of vitamin E levels, antioxidant enzymes and malondialdehyde in women with unexplained infertility

International Journal of Clinical Biochemistry and Research, 2023

Background: Unexplained infertility is a perplexing disorder. 30% of infertile couples are diagnosed with unexplained infertility with all standard fertility investigations normal. Oxidative stress has been shown to have a negative impact on reproductive function in women with endometriosis and (PCOS) polycystic ovarian syndrome. In another study antioxidant supplementation improved OS induced infertility. Many studies have demonstrated an increase in Malondialdehyde in serum of women with infertility and vitamin E as a protective antioxidant in the body with positive effect on the fertility. There is growing evidence linking OS and unexplained infertility. Based on this knowledge the specific group of unexplained infertile women were chosen to assess their levels of serum antioxidant enzymes, Vitamin E and lipid peroxidation marker. Aim: To compare the Levels of Malondialdehyde, Antioxidant enzymes and Vitamin E in the serum of women with unexplained infertility and control group. Method: Case control study. 70 normal ovulatory women who conceived within 12 months of contraceptive free intercourse, and with no history of miscarriage were recruited in the control group.70 women with unexplained infertility were recruited as study group. All participants included in the study were between 28 and 38 years of age. Serum levels of MDA, Antioxidant enzymes (GST, SOD, Catalase) and Vitamin E concentrations were compared between two groups. Vitamin E concentrations were determined by using High performance liquid chromatography. Antioxidant enzymes and MDA were measured by standard spectrophotometric assay. Results: Data was analysed using SPSS Software. Continuous parameters were analysed using Mann Whitney U test. There is a significant decrease in the vitamin E levels 3.80 (ug/ml) in the unexplained infertile group as compared to controls 6.0 (ug/ml). All women in the study group showed significantly (P<0.05) higher levels of MDA and lower levels of antioxidant enzymes such as GST, SOD and Catalase as compared to the fertile women. This decrease was found to be significant with p value of <0.05. Conclusion: The study group have a higher oxidative stress status and low level of antioxidants compared to control group. Serial measurement of oxidative stress biomarkers and their defense system may help to understand the aetiology of unexplained infertility and to enhance their chances of conception. Keywords: Oxidative stress, Unexplained infertility, Antioxidant enzymes, Lipid peroxidation marker.