Cukurova Medical Journal Vitamin C-E and Monosodium Glutamate-Induced Ovarian Toxicity Vitamin C-E ve Monosodyum Glutamata Bağlı Ovaryan Toksisite (original) (raw)
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Alexandria Journal of Medicine
Background: Monosodium glutamate (MSG) has been recognized as flavor enhancer that adversely affects male reproductive systems. Objective: The study was conducted to explore the conceivable protective effects of vitamin C and/or vitamin E on testicular toxicity induced by MSG in rats. Materials and Methods: Thirty male Wistar albino rats were divided (six per group) into: control, MSG, MSG + Vitamin C, MSG + Vitamin E and MSG + Vitamin C + Vitamin E groups. The duration of the study was three weeks. Assessment of serum testosterone, leuteinizing hormone (LH), malondialdehyde (MDA), glutathione peroxidase (GPX), interleukin-10 (IL-10) ,and tumor necrosis factor (TNF-α) were done. Histopathological examination of the testes of the rats was performed using histological, histochemical (Periodic Acid Schiff reaction (PAS)), and immunohistochemical (Proliferating cell nuclear antigen (PCNA), androgen receptors (ARs), Caspase-3) techniques. Results: MSG-group was associated with significant decrease in serum testosterone, LH, GPX, and IL-10 (P < 0.05) and significant increase in serum MDA and TNF-α (P < 0.05) when compared with control group. MSG-group revealed many histopathological changes in the testis including degeneration of the germinal epithelium, absence of sperms in the lumina of tubules, widened vacuolated interstitium, marked deposition of the collagen fibers, very strong PAS reaction and marked immunohistochemical changes. Administration of vitamin C or vitamin E significantly reduced these changes; however, the combination of vitamin C and vitamin E provided more potent protection against the toxic effect of MSG than using each vitamin alone. Also, there was insignificant difference (P > 0.05) between MSG +Vitamin C and MSG +Vitamin E groups. Conclusion: Vitamin C and Vitamin E act synergistically in reducing MSG-induced testicular toxicity via antioxidant, anti-inflammatory and antiapoptotic effects of both vitamins.
Ovarian toxicity in rats caused by methidathion and ameliorating effect of vitamins E and C
Human & Experimental Toxicology, 2007
Experimental groups were as follows: control group; a group treated with 5 mg/kg body weight MD (MD group); and a group treated with 5 mg/kg body weight MD plus vitamin E and vitamin C (MD ϩ Vit group). MD and MD ϩ Vit groups were given MD by gavage five days a week for four weeks at a dose level of 5 mg/kg/day by using corn oil as the vehicle. Serum malondialdehyde (MDA: an indicator of lipid peroxidation) concentration, serum activity of cholinesterase (ChE), and ovary histopathology were studied. The level of MDA increased significantly in the MD group compared with the control (P Ͻ 0.005). Serum MDA decreased significantly in the MD ϩ Vit group compared with the MD group (P Ͻ 0.05). The activities of ChE decreased significantly both in the MD and MD ϩ Vit groups compared with the controls (P Ͻ 0.05). However, the decrease in the MD ϩ Vit groups was less than in the MD group; the ChE activity in the MD ϩ Vit group was significantly higher compared with MD group (P Ͻ 0.05). Number of ovarian follicles were significantly lower in the MD group compared to the controls (P Ͻ 0.05). Number of atretic follicles were significantly higher in the MD group than in the controls (P Ͻ 0.05). Follicle counts in MD ϩ Vit group showed that all types of ovarian follicles were significantly higher, and a significant decrease in the number of atretic follicles compared with the MD group (P Ͻ 0.05). In conclusion, subchronic MD administration caused an ovarian damage, in addition, LPO may be one of the molecular mechanisms involved in MD-induced toxicity. Treatment with vitamins E and C after the administration of MD reduced LPO and ovarian damage. Human & Experimental Toxicology (2007) 26, [491][492][493][494][495][496][497][498]
Ain Shams journal of forensic medicine and clinical toxicology, 2013
Background: Monosodium glutamate (MSG) is a commonly used food enhancer which causes a wide range of endocrinal disorders including reduced fertility rate in both sexes. Earlier studies have shown that chronic administration of MSG induces oxidative stress in some organs of experimental animals. Accordingly, this work was carried out to study the possible ameliorative effect of vitamin C on monosodium glutamate induced testicular toxicity in adult male albino rats. Study design: Sixty adult male albino rats were included in this study and divided into four main groups. Group I used as control group (10 rats). Group II (10 rats) received 100mg/kg/day vitamin C. Group III (20 rats) was subdivided into two subgroups (10 rats each): 100mg/kg/day and 4gm/kg/day MSG treated rats. Group IV (20 rats) was also subdivided into two subgroups and received both MSG and vitamin C in their previous doses. All animals were treated orally by gastric tube for two months. Body weight, serum testosterone level, cauda epididymal sperm reserves, histopathological examination using H&E stain and survivin immunostaining were studied. Results: There was a significant increase in the body weight (p<0.05) and in the percentage of sperm abnormal forms (p<0.05), while a significant reduction in the sperm count and motility (p<0.05) was noticed. These results were dose dependent. Animals treated with 4gm/kg MSG showed severely damaged seminiferous tubules with few primary spermatocytes in H&E stained sections and marked reduction in survivin expression in histochemical study compared to other groups. Moreover, there was significant improvement in all studied parameters on administration of vitamin C concomitant with MSG which was more observed with low MSG dose (100mg/kg). Thus, vitamin C holds a promise as an agent that can reduce MSG induced toxic effects in testes.
Biomarkers and Genomic Medicine, 2015
This study aimed to investigate whether combined supplementation with vitamin C and vitamin E was able to modify the superoxide dismutase (SOD) and malondialdehyde (MDA) levels in the ovarium of rats exposed to rhodamine B. Twenty-five female Wistar albino rats were divided into five groups (n = 5 each), including control (untreated group); rhodamine B group; rhodamine B group which received vitamin C (0.2 mg) + vitamin E (0.04 IU/g body weight); rhodamine B group which received vitamin C (0.4 mg) + vitamin E (0.04 IU/g body weight); and the rhodamine B group which received vitamin C (0.8 mg) + vitamin E (0.04 IU/g body weight). Analysis of MDA levels as a marker of lipid peroxidation was done spectrophotometrically. Analysis of SOD levels was done by enzyme-linked immunosorbent assay technically. Endometrial histology was analyzed in hematoxylin eosin staining. This increase in ovarian MDA was significantly (p 0.05). All doses also significantly prevented rhodamine B-induced decr...
Journal of Toxicology, 2016
A study was designed to investigate ameliorates effect of combined vitamins C and E able to against depot-medroxyprogesterone acetate- (DMPA-) induced ovarian oxidative stress in rat. Twenty-five female Wistar rats were divided into the following groups (n=5rats each): control (untreated) (C); depot-medroxyprogesterone acetate (DMPA); DMPA plus green vitamin C (at dose of 0.2 mg/gram; 0.4 mg/gram; 0.8 mg/gram) and vitamin E (0.04 IU/gram). The treatment with combined vitamins C and E was performed for four weeks. Analysis of malondialdehyde (MDA) level as a marker of oxidative stress was done colorimetrically. Analysis of SOD level was done by enzyme linked immunosorbent assay (ELISA) technically. This increase in ovarium MDA was significantly (P<0.05) attenuated by medium dose treatments of combined vitamins C and E. DMPA insignificantly decreased SOD levels compared to the untreated group. This decrease in ovarian SOD level was significantly attenuated by all doses of the combi...
Annals of Medical Research
The aim of the present study is to investigate the effects on ovarian tissues of adult female rats, which were the offspring of rats administered acrylamide (AA) and vitamin E during pregnancy. Material and Methods: Thirty rats were divided into 5 groups of 6 pregnant rats, as the Control, Corn Oil, Vitamin E, AA, Vitamin E + AA groups. The births were monitored on the 21 st day to select the female rats. The selected female rats were decapitated at the end of the 8 th week and their ovarian tissues were removed under anesthesia malondialdehyde (MDA), reduced glutathione (GSH), total antioxidant capacity (TAS), total oxidant capacity (TOS), oxidative stress index (OSI), superoxide dismutase (SOD), catalase (CAT) and nitric oxide (NO) levels were examined. Results: Administration of AA during pregnancy caused an increase in MDA, TOS, OSI and NO levels and a decrease in GSH, SOD, CAT and TAS levels in the ovarian tissues of the rats when compared to the control group. It was determined that vitamin E administration caused an increase in GSH, SOD, CAT and TAS levels in ovarian tissues, compared to all other groups (P <0.05). Conclusion: Exposure to food-induced AA toxicity increases each day and the parallel increase in infertility suggests that it could be related to AA toxicity. Although vitamin E is capable to exert a protective effect against AA toxicity through increasing the antioxidant capacity of ovarian tissue, there is certain necessity for further studies.
Journal of Physics: Conference Series, 2019
Factors causing infertility include unhealthy lifestyle, such as excessive use of MSG which can produce free radicals and can affect morphology and motility of spermatozoa. Antioxidant is needed to prevent free radicals, which can be obtained from various sources such as tomatoes and zinc. This study was used experimentally with post test only control group design with divided into 5 groups (K1 to K5). K1 were exposed standard feed, K2 were exposed to 4 mg/day of MSG, K3 were exposed 3.7 g/day of tomatoes + 4 mg/day of MSG, K4 were exposed 0.27 mg/day of zinc + 4 mg/day of MSG and K5 were exposed combination of 3.7 g/day of tomatoes + 0.27 mg/day of zinc + 4 mg/day of MSG. Tomatoes or zinc and the combination of tomatoes and zinc were effective to improve the morphology and motility of male white rats Rattus norvegicus spermatozoa which were exposed to MSG. The combination of tomatoes and zinc were the most effective in improving morphology and motility of spermatozoa exposed to MSG.