In vivo Hepatoprotective and Antioxidant Activity of Enicostemma littorale against Ccl4 Induced Liver Damage in Rats (original) (raw)
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African Journal of Agricultural Research, 2007
The hepatomodulatory response of ethanol extract of Enicostemma littorale Blume was examined in contrast to oxidative stress-induced liver injury by carbon tetrachloride (CCl4) in albino wistar male rats. The rats received the extract, orally at the doses of 125, 250 and 500 mg/kg b wt/day for 21 consecutive days and CCl4, at the dose of 0.2 ml/kg b wt/twice a week, i.p with olive oil, in the ratio 1:1 treatment. The rats subjected only CCl4 with olive oil, showed remarkable oxidative stress-induced liver injury. Supplementation of E. littorale extract significantly (P< 0.001) increased the hepatic reduced glutathione (GSH), glutathione-s-transferase (GST), glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT) and vitamin-C (vit. C) in the liver, with a dose-dependent reduction of the thiobarbituric acid reactant substances (TBARS) as evidenced by reduced hepatic lipid peroxidation (LPO) levels, compared with the control animals. The hepatic total cholesterol a...
Enicostemma axillare is used in Indian traditional medicine as a liver tonic. Its ethyl acetate extract has shown potent in vitro antioxidant activity and found to contain 7.26% of a bitter secoiridoid glycoside, swertiamarin. Hence, in the present study the ethyl acetate extract was screened for hepatoprotective and antioxidant properties against CCl 4 induced hepatic injury in rats. The hepatoprotection was assessed in terms of reduction in histological damage and changes in serum enzymes and metabolites. The pretreatment with the extract at 100 and 200 mg/kg body weight doses given orally for eight days prior to CCl 4 caused significant restoration of altered biochemical changes due to CCl 4 towards the normal in serum, liver and kidney. The extract treatment at 200 mg/kg body weight was found to be more potent than the standard silymarin at 100 mg/kg body weight in reversing most of the biochemical parameters. Histopathological studies complemented the results of biochemical estimations in providing a proof of hepatoprotective and antioxidant actions of the extract. The study provides a support to the ethnomedical use of E. axillare in India.
Hepatoprotective activity of Enicostemma littorale in CCl $ _4 $-induced liver damage
2004
Vellarugu or Vallari in Tamil [3]. E. littorale plant has been used as a folk medicine for the treatment of diabetes mellitus in Western and Southern India [4]. Ethnomedical studies of North Gujarat (India) reveal the use of hot aqueous extract of E. littorale by the tribal inhabitants for the treatment of diabetes, fever, stomach ache, dyspepsia and malaria in interior part of Gujarat. Studies from our laboraory have
International Journal of Pharmacology, 2008
This study was designed to scrutinize the enzymatic and nonenzymatic antioxidant activities of the methanolic extract of whole plant of Cleome viscosa and Cleome gynandra (MeCV and MeCG) in streptozotocin (STZ) induced diabetic rats. Oral administration of MeCV, MeCG and Me.CV+CG combination at a dosage of 400 mg/ kg b.w for 28 days on diabetic rats. The enzymatic and non-enzymatic antioxidant assays were measured by using standard procedures in liver tissue of diabetic and normal rats. There is a significant (p < 0.05 and p < 0.01) improvement in the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione-s-transferase (GST) in liver tissue of STZ induced diabetic treated rats when compared with the untreated diabetic rats and the contents of ascorbic acid (AA) and glutathione (GSH) levels were also significantly increased in the treated diabetic rats when compared with untreated diabetic rats. These results clearly indicated that the whole plants of MeCV have good hepato protective activity than MeCG whole plant in STZ induced diabetic rats.
Journal of Basic and Clinical Physiology and Pharmacology, 2012
Background: Liver diseases are on rise and remain a serious health problem. Olax subscorpioidea and Distemonanthus benthamianus are two plants used in Ivorian traditional medicine in the treatment of many diseases including jaundice and hepatitis. Objective: The present study was carried out to assess the hepatoprotective and in vivo antioxidant potentials of the hydro-ethanolic leaf extracts of these plants in carbon tetrachloride (CCl 4)-induced hepatotoxicity in rats. Materials and Methods: The plant extracts at doses of 25 and 100 mg/kg bw, and silymarin (25 mg/kg bw) were intraperitoneally (i.p.) injected once daily for 7 days to different groups of rats. Hepatotoxicity was induced on the 7 th day in all the group animals except control. Rats were sacrificed on the 8 th day and blood was collected. Serum biochemical parameters and antioxidant activity were measured using standard procedures. Histopathological examinations of liver rats were also performed. Results and Discussion: Hepatotoxicity induced with CCl 4 was well manifested by significant increase in serum activities of GOT, GPT, ALP and GGT, and enhancement of total bilirubin and TBARS levels. On the other hand, the level of total protein, albumin, α 1-globulin significantly decreased, and DPPH-free radical scavenging activity and TAP were lowered. Pretreatment with plant extracts and silymarin prevent the toxic effects of CCl 4 by decreasing serum enzyme activities, total bilirubin and TBARS levels and improving serum TAP and DPPH-free radical scavenging potential. Histopathological observations showed almost normal hepatic cells with a mild degree of inflammation, lesser fatty infiltration and absence of necrosis among the rats treated at 100 mg/kg of extracts of both the plants. Conclusion: The results suggest that the hydro-ethanolic leaf extracts of O. subscorpioidea and D. benthamianus possesses hepatoprotective and in vivo antioxidant activity.
Experimental and Toxicologic Pathology, 2011
A model of hepatotoxicity by carbon tetrachloride (CCl 4) in rats was used in order to evaluate the protective potential of the acetonic and methanolic extracts of Heterotheca inuloides. Pretreatment with the two H. inuloides extracts attenuated the increase in the activity of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) observed in CCl 4-induced liver injury. The protective effect was confirmed by the analysis of tissue slides stained with hematoxylin-eosin and periodic acid/Schiff's reagent. Additionally, the two extracts are scavengers to the superoxide radical as was observed by electron paramagnetic resonance. Due to the fact that the methanolic extract resulted in a better protective effect in the previous experiments, it was used to investigate in more detail the mechanism of hepatoprotection. Quercetin, one of the main components of the extract, with known hepatoprotective and antioxidant activity was used as a positive control. Pretreatment of animals with the methanolic extract or quercetin, was associated with the prevention of 4-hydroxynonenal and 3-nitrotyrosine increase in the liver, two markers of oxidative stress. Furthermore, the decrease in the activity of several antioxidant enzymes including superoxide dismutase, catalase and glutathione peroxidase in CCl 4-induced liver injury was alleviated by the pretreatment with H. inuloides methanolic extract or quercetin. These results suggest that the hepatoprotective capacity of H. inuloides methanolic extract is associated with its antioxidant properties, which would also explain the biomedical properties attributed to this plant.
Indo American Journal of Pharmaceutical Research, 2018
The present study is aimed at evaluating the chemoprotective effect of Enicostemma littorale in DEN induced hepato-carcinogenesis in Sprague Dawley rats. The preliminary steps involved extraction, phytochemical investigation, HPTLC study and In vitro antioxidant activity using DPPH, ABTS. and Initiation of HCC was done by single i.p injection of DEN at a dose of 200mg/kg. The MEEL received treatment for 90 days after 14 days of development of HCC and continued for entire study period, whereas the other two group given normal saline, 5-flurouracil (20mg/kg) i.p. The results showed that the injection DEN lead to the development of liver tumors in rats. Significant effect of serum biochemical parameter like SGOT, SGPT, ALP, UREA, TOTAL PROTEIN and tumor marker was observed with depletion of endogenous antioxidants SOD, CAT, GSH, there by leading to higher LPO. The result exhibited that MEEL treatment (Preventive) group offered excellent shielding against HCC and displayed all the parameter in near normal range with a maintained antioxidant enzyme system. The result obtained showed that extracts were found to containing phenols at a concentration of 70.25 mg/g and flavonoids 26.03mg/g. HPTLC Analysis showed presence of Quercetin, Mangiferin, Gallic acid, Catechin and sweroside. In the DPPH, ABTS, FRAP, TRAP radical scavenging assay MEEL has displayed the highest antiradical activity in both assays and was also comparable with the standard qucertein The present study reveal the efficacy of the MEEL to prevent malignancy induced by chemical carcinogen and the phytoconstituents responsible for activity.
2014
Effects of n-butanol fraction of Gongronema latifolium leave extract on some liver function and histological parameters in CCl 4-induced oxidative damage in Wistar albino rats were assessed. Fifty-four (54) Wistar albino rats were divided into treatment group and LD 50 groups. Group A (normal control) was given feed and water, Group B (vehicle control) was injected with olive oil intraperitoneally, while the rest groups (C, D, E, F and G) were injected intraperitoneally with a single dose of CCl 4 (148 mg/kg) as a 1:1 (v/v) solution in olive oil and all the animals were fasted for 36 hours. This was repeated once every week for a period of four (4) weeks. At the end of 28 days of treatment, liver marker enzymes studies showed that there was significant (p<0.05) increase in the serum activities of ALT, AST, ALP and bilirubin concentrations in CCl 4-induced control group when compared with the normal control and induced treated groups but there was no significant (p>0.05) difference of these liver marker enzymes and bilirubin levels between the normal control and induced treated groups. Antioxidant assay on the liver homogenate showed that there was significant (p<0.05) decrease in SOD, CAT, GPx and a significant increase (p<0.05) in MDA of CCl 4-induced control rats when compared to the normal control rats but there was no significant (p>0.05) difference between the normal control and induced treated groups. These findings suggested that n-butanol fraction of methanolic leave extract of G. latifolium may have anti-hepatotoxic and antioxidative effects against CCl 4-induced liver damage in rats.
The hepatoprotective and antioxidant activity of Bauhinia hookeri ethanol extract (BHE) against CCl 4-induced liver injury was investigated in mice. BHE was administered (500 and 1000 mg/kg/day) along with CCl 4 for 6 weeks. The hepatic marker enzymes: alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were determined in the serum. The antioxidant parameters: glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione transferase (GST), and malondialdehyde (MDA) were estimated in the liver homogenate. BHE treatment significantly inhibited the CCl 4-induced increase in ALT (44 and 64%), AST (36 and 46%), ALP (28 and 42%), and MDA (39 and 51%) levels at the tested doses, respectively. Moreover, BHE treatment markedly increased the activity of antioxidant parameters GSH, GPx, GR, GST, and SOD. Histological observations confirmed the strong hepatoprotective activity. These results suggest that a dietary supplement of BHE could exert a beneficial effect against oxidative stress and various liver diseases by enhancing the antioxidant defense status, reducing lipid peroxidation, and protecting against the pathological changes of the liver. The hepatoprotective activity of BHE is mediated, at least in part, by the antioxidant effect of its constituents. The active constituents of BHE were identified by HPLC-PDA-ESI/MS/MS.
The combined antioxidant and hepatoprotective effect of Eclipta prostrata was evaluated against carbon tetra chloride (CCl 4 ) induced hepatic damage in wistar albino rats. Ethanolic extract from the Eclipta prostrata at a dose level of 200mg/kg of body weight was administered orally daily once for 15 days. The substantially elevated serum marker enzymes such as Aspartate transaminase (AST), Alanine transaminase (ALT), Alkaline phosphatase (ALP), Acid phosphatase (ACP) and the antioxidant enzymes such as glutathione peroxidase, superoxide dismutase and catalase were found due to CCl 4 treatment. The levels of above mentioned enzymes were brought to near normalcy after administration of plant extract. The biochemical parameters like total protein, total bilirubin were also restored towards normal levels. In addition Eclipta prostrata significantly decrease the liver weight of CCl 4 intoxicated rats. Silymarin at a dose level of 25mg/kg was used as a standard reference drug for comparison.. The results of this study strongly indicate that Eclipta prostrata is having a potent hepatoprotective action against CCl 4 induced hepatic damage in rats.