Evaluation of Hypoglycemic and Hypolipidemic Activities of Zizyphus Jujuba (original) (raw)
Related papers
Objective: The present study was designed to evaluate the hypoglycemic and hypolipidemic activity of methanolic extract of zizyphus jujuba leaves in normal and streptozotocin inducing diabetic rats. Zizyphus jujuba Lam. is also called as Baer tree, belongs to the family Rhamnaceae. It is used primarily for its fruits. Jujube, a delicious fruit, is an effective herbal remedy improving stamina and muscular strength and aids weight gain, strengthens liver function and increases immune system resistance. Methods: The dried bark was powdered and extracted with various solvents by successive soxhlet hot extraction process with increasing order of polarity. On phytochemical investigation, the methanol extract and aqueous extract has shown steroids, flavonoids and tannins. The zizyphus jujuba was reported for the many of biological activity, and hypoglycaemic activity of the same has not been reported so far. Streptozotocin is used to induce diabetic in rats and the blood lipid levels were estimated using commercial kits available in the market. The methanolic extract of Zizyphus jujuba was administered at the doses 100mg/kg and 200mg/kg. Results : The methanolic extracts of Zizyphus jujuba 100mg/kg and 200mg/kg produced significant decreased p<0.01) in blood glucose level after 2,4 and 6 hour of treatment as compared to untreated diabetic rats respectively as 503±3The treatment of diabetic rats with (Group III) extracts for 3 weeks resulted in significant decrease of serum triglycerides, total cholesterol and LDL-c cholesterols as compared to untreated diabetic rats (Group II) and the values came down significantly (p<0.01 ) below those in the normal healthy control group .
2020
Zizyphus jujuba Lam. Is also called as Baer tree, belongs to the family Rhamnaceae. The dried bark was powdered and extracted with various solvents by successive soxhlet hot extraction process with increasing order of polarity. On phytochemical investigation, the methanol extract and aqueous extract has shown steroids, flavonoids and tannins. The present study was designed to evaluate the hypoglycemic and hypolipidemic activity of methanolic extract of zizyphus jujube leaves in normal and streptozotocin inducing diabetic rats. The zizyphus jujube was reported for the many of biological activity, and hypoglycaemic activity of the same has not been reported so far. Streptozotocin is used to induce diabetic in rats and the blood lipid levels were estimated using commercial kits available in the market. The methanolic extract of Zizyphus jujube was administered at the doses 100mg/kg and 200mg/kg doses. Both the doses caused a significant decrease in the levels of total cholesterol, trig...
The Effect of Ziziphus Jujuba Fruit Extract in Diabetic and Non-Diabetic Rat
Iranian Journal of Diabetes and Obesity, 2014
Herbal medicine and medical plants such as Ziziphus Jujuba are widely used in the treatment of diseases such as diabetes mellitus. We investigated the effects of ethanol extracts of Ziziphus Jujuba fruit on serum glucose, triglycerides, LDL-cholesterol, HDL- cholesterol, cholesterol and activities of aminotransferase enzymes in streptozocin-induced diabetic adult male rats . Materials and Methods: Adult Wistar rats were rendered hyperglycemic. Animals were divided into four equal groups (7 per group). Animals in all groups were treated for 14 days. Blood samples were collected from the heart of the animals. Results: Continuous supplementation of ethanol extract in drinkable water of diabetic rats resulted to a significant decrease of fasting blood glucose, cholesterol and increase in high density lipoprotein levels after 14 days (P 0.05). Conclusion: This paper discussed the antidiabetic effect of an alcoholic extract of Ziziphus Jujuba on streptozocin-induced diabetes in rats. Rese...
A Review on Zizyphus as Antidiabetic
2012
The present review paper aims to highlight the effects of extracts of genus zizyphus for the treatment of Diabetes clinically throughout the world. The basic aim is to study the hyperglycemic effect of this genus and its use. So as the effect of herbal medicine is more friendly then synthetic medicines. The effects of three species Ziziphus spinacristi, Zizyphus jojoba, and Zizyphus vulgaris have been studied clinically and have great effect on insulin secretion. While work on Zizyphus maritiana have also been done. It is concluded that genus zizyphus have potential of antidiabetic activity. Many species of Zizyphus grow widely. As natural drugs are with no or less side effects and safe for human health so they can greatly replace synthetic drugs in the forth coming future.
The Egyptian Journal of Hospital Medicine, 2009
Background:The present study aims to investigate the hypoglycemic, hypolipidimic and antioxidant effect of the methanolic crude extracts of Zizyphus spina christi, Morus alba and Olea europaea leaves, individually or in combination against diabetes induced rats by Streptozotocin (STZ). Results:Hyperglycemia and hyperlipidaemia except in high density lipoproteins (HDL) were observed in serum after 5 weeks of STZ administration. This was associated with a depression in hepatic glutathione (GSH) concentration as well as hepatic catalase (CAT), glutathione-stransferase (GST) and superoxide dismutase (SOD) activates. In addition hepatic thiobarbituric acid-reactive substance (TBARS) and protein carbonyl (PC) were significantly elevated, indicating increased lipid and protein oxidation and oxidative stress. Depression in blood hemoglobin (Hb) content, serum insulin levels, total antioxidant capacity (TAOC) and nitric oxide (NO) levels as well as body weight gain were also observed in diabetic rats. Administration of 100mg/kg alcoholic extracts of Zizyphus spina christi, Morus alba and Olea europaea leaves 3 days before and after STZ injection daily for 5 weeks significantly ameliorated the oxidative stress evidenced by lowering TBARS & PC as well as increasing hepatic GSH concentration and CAT, GST and SOD activates as compared with STZ treated rats. These effects were paralleled with marked protection against STZ induced hyperglycemia and disturbance of lipid profile. They also caused a great improvement in insulin levels, TAOC, NO, Hb content and body weight gain. Conclussion:Thus, these results showed that the administration of the crude extracts of either Zizyphus spina christi, Morus alba or Olea europaea leaves individually or in combination might improve the clinical manifestation of diabetes and decrease the oxidative stress, this study supports the beneficial effects of these extracts especially Zizyphus spina christi, which showed marked amelioration and this may be attributed to the presence of saponin glycosides which have an inhibitory effect of serum glucose level in addition to enhance the cellular antioxidant defense. This activity contributes to the protection against oxidative damage in STZ induced diabetes.
Antidiabetic and Antioxidant Activities of Zizyphus lotus L Aqueous Extracts in Wistar Rats
Journal of Nutrition & Food Sciences, 2011
Objectives: Zizyphus lotus L. is a medicinal plant, used in traditional medicine for its multiple therapeutic properties. The aim of the present study was to determine the antidiabetic and antioxidant effects of aqueous extracts of different parts of Zizyphus lotus in diabetic Wistar rats. Research methods and procedures: The male Wistar rats were rendered diabetic by intraperitoneal (i.p.) injection of streptozotocin (40 mg/kg body weight). Aqueous extracts from different parts, i.e, root, leaf, and seed of Zizyphus lotus L. were prepared and administrated orally to the animals at the dose of 300 mg/kg for 21 days. Blood glucose level was measured, and antioxidant status was assessed by determining oxygen radical absorbance capacity (ORAC) and the activities of enzymes like catalase, glutathione reductase and peroxidase in pancreas, liver, and erythrocytes. Vitamin C levels were determined by precipitating with 10% trichloroacetic acid. Vitamins A and E concentrations were measured by HPLC. Results: The leaf and root, but not seed, extracts exerted the glucose lowering effect on 21 st day of postadministration. The leaf and root extracts corrected antioxidant status of diabetic animals in pancreas, liver and erythrocytes. The concentrations of different vitamins (vitamin A, C and E) in diabetic rats were also modulated by leaf and root, but not seed, extracts. Conclusion(s): Our study shows that oral administration of Zizyphus lotus L. extracts from roots and leaves exerted antidiabetic and antioxidant effects in diabetic rats. Z. lotus L. seems to be a good candidate to lower, in addition to conventional antidiabetic drugs, the hyperglycaemia in diabetic subjects.
The use of plant products in the management of diabetes has gained ground in pharmacotherapy. It becomes imperative to evaluate the antidiabetic effects of fruit extracts of Ziziphus mauritiana (HMZM), Ziziphus spina christi (HMZS) and Glibenclamide on blood glucose, total protein, albumin and lipid profile in alloxan induced diabetic rats. 68 albino rats weighing 70-130g were used in this study. 26 rats were used for acute toxicities of Ziziphus mauritiana and Ziziphus spina christi. 42 rats of 7 groups of 6 rats each were used to test antidiabetic effects of Ziziphus mauritiana and Ziziphus spina christi plant extracts. Group 1 served as the negative control, groups 2-7 were intraperitoneally administered 360mg/kg of alloxan in normal saline respectively. Group 2 served as positive control, groups 3 and 4; 5 and 6 were respectively administered daily dose of 200 and 400mg/kg of HMZM and HMZS, group 7 was administered 0.21mg/kg of glibenclamide. Results showed Ziziphus mauritiana and Ziziphus spina christi non toxic at dose of 5000mg/kg. 48 hours after alloxan administration, blood glucose levels were found to be significantly higher (P<0.05) in groups 2-7 compared to group 1, thus confirming induction of diabetes. In groups 3-7, on 7 th and 14 th days of administration of extracts and Glibenclamide, there was a significantly lower (p<0.05) serum glucose, triacylglycerol, High density Lipoprotein, Low density lipoprotein and significantly higher (P > 0.05) serum level of total protein and albumin compared to group 2. The results obtained showed a significantly lower (P < 0.05) serum level of glucose. The effects of HMZM and HMZS fruit extracts on blood glucose, total protein, albumin and lipid profile was dose dependent. Conclusively, this study has demonstrated the antidiabetic effects of HMZM and HMZS with HMZS having a more pronounced effect on Blood glucose and lipid profile. Cite this article as: Abubakar SM, Umar SA, Alexander I, Abubakar N, Abdulazeez MA, Sule MS, Evaluation of hypoglycaemic, hypolipidaemic and non toxic effect of hydro-methanolic extracts of Ziziphus mauritiana, Ziziphus spina christi fruit and glibenclamide on alloxan induced diabetic rats, Journal of Drug Delivery and Therapeutics. 2018; 8(3):82-92
Lancet, 2011
Aim of the study: The present study aimed to evaluate the anti-diabetic activity of Zizyphus spina-christi leaf extract (200 mg/kg b.w.), plain and formulated in STZ-diabetic rats. Percentage yield of extracts, marker yield (christinin-A) and antihyperglycemic potencies, depending on seasonal variation were investigated. The chemical stability, study of storage conditions, shelf life T90 prediction of both plain extract and formulated soft gelatin capsules by accelerated studies were studied. Material and methods: Changes in all studied parameters after oral administration of Z. spina-christi extract for 28 days were reported. Seasonal variation affecting yield and activities was studied. Flavonoid contents were HPLC evaluated. The capsules were stored at 30, 40 and 50 • C [75% relative humidity] and their residual christinin-A content was assayed for 24 weeks. Christinin-A chemical degradation was monitored by HPLC stability indicating method previously validated. Possible physical examination was checked by dissolution test of the content of the capsules using dissolution tester USP XXIV. Result: Oral administration of Z. spina-christi leaf extract, plain and formulated for 28 days reduced blood glucose level with significant increase in serum insulin and C-peptide levels. Marked elevation in total antioxidant capacity with normalization of percentage of glycated hemoglobin (HbA1C%) was reported. Moreover, they succeeded to reduce the elevated blood lactate level and to elevate the reduced blood pyruvate content of diabetic rats. In line with amelioration of the diabetic state, Zizyphus extract, plain and formulated restored liver and muscle glycogen content together with significant decrease of hepatic glucose-6-phosphatase and increase in glucose-6-phosphate dehydrogenase activities. In vitro experiments showed a dose-dependent inhibitory activity of Zizyphus extract against ␣-amylase enzyme with (IC 50 ) at 0.3 mg/ml. Such finding has been supported by the in vivo suppression of starch digestion and absorption by Zizyphus extract in normal rats.
International Journal of Pharmacognosy and Phytochemical Research
In the current study the phytochemical profile of 70% ethanol extract of Ziziphus jujubа (Rhamnaceae), cultivated in Bulgaria has been investigated. The fruit extract contained numerous phytochemicals, such as triterpenes, phenolic acids and flavonoids. Five triterpenes, 11 phenolic acids and 5 flavonoids have been identified. The fruit extract had a total phenolic content of 21.62 ± 0.0265 mg/g and total flavonoid content 1.34 ± 0.017 mg/g dried extract. Among the triterpenes with the highest concentration was the betulinic acid 20943.17±527.06 µg/g dried extract, rosmarinic acid (1174.26±29.55 µg/g) among the phenolic acids, followed by myricetin (214.61±5.40 µg/g) as a representative of flavonoids and rutin 3 046.89±76.68 among the quercetin glycosides. The inhibitory effect of Z. jujubа fruit extract has been investigated on the key enzymes linked to diabetes – α-glucosidase and α-amylase. In order to evaluate the type of inhibition a Lineweaver-Burk plot was produced. The resul...
Antidiabetic activity and toxicity of Zizyphus spina-christi leaves
Journal of Ethnopharmacology, 2005
The effect of the butanol extract of Zizyphus spina-christi (L.), Willd (Rhamnaceae) leaves and its major saponin glycoside, christinin-A, on the serum glucose and insulin levels was studied in non-diabetic control, type-I (insulin-dependent) and type-II (non-insulin-dependent) diabetic rats. Pretreatment either with 100mg/kg butanol extract or christinin-A potentiated glucose-induced insulin release in non-diabetic control rats. In type-II but not in type-I