PENETAPAN KADAR FENOLIK TOTAL DARI EKSTRAK ETANOL BUNGA ROSELLA BERWARNA MERAH (Hibiscus sabdariffa L.) DENGAN MENGGUNAKAN SPEKTROFOTOMETRI UV-Vis (original) (raw)
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Jurnal Fitofarmaka Indonesia
One of the herb plants, purple Roselle flowers (Hibiscus sabdariffa L), used to traditionally for various diseases. The aim of this research is to determine the total phenolic content of ethanol extract of purple Roselle flowers. Sample as many one kilograms extracted by maceration method using ethanol 96%. Resulty 120 grams thick extract. Total phenolic content determined by UV-Vis spectrophotometer at wave leght 743 nm with gallic acid as standard reference with Folin-Ciocalteau reagent. The result show the rendamen of ethanolic extract 12% with total phenolic content 1.6726 %.
Jurnal Fitofarmaka Indonesia, 2016
Red Roselle (Hibiscus sabdariffa L.) is a medicinal plant used by the public as drugs. This plant is efficacious because one contains chemical constituents are flavonoids. Comparative Study of total Flavonoid content in red Roselle (Hibiscus sabdariffa L.) from North Luwu district of South Sulawesi province and the province of East Java Kediri district has done. The aim of study to determine of total flavonoid content in Kediri regency and district of North Luwu using a spectrophotometer. Extraction is done by maceration method using 96% ethanol. Phytochemical screening using the color reaction identification and determine content of Colorimetric method using UV-Vis spectrophotometry at the maximum wavelength of 417 nm. Meanwhile the yield extract red Rosella from Kediri (sample I) 12.703% and red Rosella from north Luwu (sample II) 10.44%. Test phytochemical to sample I and sample II positive containing Flavonoids. Test total Flavonoid content to sample I is 0.02816% and sample II is 0.2075%.
Total phenolic content and antioxidant activity of roselle (Hibiscus sabdariffa) extracts
2019
Introduction: Roselle (Hibiscus sabdariffa) is widely known for its benefits and medicinal properties. The aims of this study were to determine the total phenolic content (TPC) in H. sabdariffa extract, its antioxidant activity and relationship between TPC and its antioxidant scavenging capacity. Methods: The TPC value for each extract of free, bound soluble and bound insoluble phenolic acids was determined by using Folin-Ciocalteu assay. The antioxidant scavenging capacity was evaluated by using DPPH assay. Results: The results obtained in this study showed that bound soluble phenolic acids (7.63 + 0.28 mg GAE/g DW) had the highest TPC value than the free and insoluble phenolic acid extracts (2.78 + 0.20 mg GAE/g DW and 3.61 + 0.85 mg GAE/g DW, respectively) (p<0.05). As for the IC50 values, bound soluble phenolic acid extract exhibited the greatest value(0.62%) as compared to the free and bound insoluble phenolic acid extracts (0.90% and 1.14%, respectively). A positive correla...
Horticulturae
Roselle (Hibiscus sabdariffa L.), as an edible flower, has long provided an array of positive effects on human health. This benefit is a result of phenolic compounds that are naturally present mainly in the calyx. Plentiful medicinal remedies and functional foods based on this flower are available worldwide, as supported by the studies of phenolic compounds in recent decades. This paper aims to provide a comprehensive review of the composition, biological activity, and beneficial effects on human health of phenolic compounds in roselle. This review was performed in accordance with the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) guidelines. A structured search in the published literature for phenolics compositions in roselle was required prior to the evaluation on the validity of the reported analytical methods. Reliable identification and quantification of phenolic compounds in roselle can be achieved by employing the proper extraction and separation ...
Acta Horticulturae, 2016
An increased consumption and demand in international trade suggests a need for quality assurance of roselle (Hibiscus sabdariffa) products. A liquid chromatography-mass spectrometry method was developed for the determination of anthocyanin profiles of the calyces, phenolic acids and flavonoids of the leaves of Jamaica, Senegal and Malaysia accessions. Delphinidin-sambubioside, delphinidinglucoside, cyanidin-sambubioside, cyanidin-glucoside and delphinidin were identified from the calyces of these accessions. Chlorogenic acid and its isomers, glycosylated derivatives of quercetin and kaempferol, and 5-(hydroxymethyl) furfural were identified from the leaves of these accessions. No significant differences in anthocyanins among accessions were found, except the accession from Malaysia, which contained significantly (P<0.05) higher amounts of delphinidin-glucoside and delphinidin. The total phenols determined by Folin-Ciocalteu's assay were 15.92+1.12, 17.63+0.7, and 19.25+1.10 mg g-1 for the calyces of Jamaica, Malaysia and Senegal, respectively, and 23.76+2.16, 22.28+1.77 and 20.62+1.61 mg g-1 for the leaves of Malaysia, Senegal and Jamaica, respectively, calculated as gallic acid equivalents. The Senegal accession contained a higher (P<0.05) amount of total phenols in calyces than the Jamaica accession. The antioxidant activities determined by the ABTS radical cation decolorization assay were 64.38+1.56, 60.36+3.34 and 62.49+2.69 mg g-1 for the calyces and 111.43+7.17, 121.84+12.55 and 110.68+10.27 mg g-1 for the leaves of Malaysia, Senegal and Jamaica, respectively, as Trolox equivalents. Macro-and micronutrient elements varied significantly (P<0.05) among accessions. This study reveals variability in phenolic, antioxidant and elemental nutrient contents and suggests that the selection of roselle accessions can influence the quality of developed products.
Antioxidant Activity of Red and Purple Rosella Flower Petals Extract (Hibiscus sabdariffa L.)
Journal of Pharmaceutical Research International
Aims: This study aims to test the antioxidant activity of red and purple H. sabdariffa flower petals extract and conduct qualitative phytochemical screening. Study Design: Antioxidant potential of red and purple H. sabdariffa flower petal extract analyzed by spectrometric assays. Place and Duration of Study: This study was carried out at School of Pharmacy Muhammadiyah Cirebon, Cirebon, West Java, Indonesia from the year of 2020 to 2021. Methodology: Red and purple H. sabdariffa petals extracted with 70% ethanol. The extract was then examined for its antioxidant activity using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method, after the qualitative phytochemical screening. Results: In this study, the concentration of red and purple H. sabdariffa petals extract dependently demonstrated the ability to scavenge DPPH. In the DPPH radical scavenging activity test, the red and purple H. sabdariffa petasl extracts designating IC50 values of 63.77 and 37.19 µg/ml and fall into the category of...
African Journal of Biotechnology
Build-up of free radicals in the human body can cause oxidative stress which may invariably lead to degenerative diseases and eventual death. Antioxidants such as polyphenols and flavonoids found in fruits and vegetables have been shown to protect the body against the damaging effects of these free radicals. Roselle contains high amounts of antioxidants and its leaves are often used for sauces or in herbal preparations to treat certain ailments. Several landraces of roselle occur in Ghana but have not been screened for antioxidant activity. Leaf samples of roselle acessions were harvested 60 days after sowing, freeze dried and analysed for total phenolic content (TPC), total flavonoid content (TFC) as well as total antioxidant activity (TAA) using UV-VIS Spectrophotometer (Shimadzu, 1201, Japan). No statistically significant differences were observed in TPC which ranged from 20 ± 0.6 µg/g (RNL) to 90 ± 0.6 µg/g (Sob-4). TFC and TAA in the leaf samples; however, showed statistically significant variation and ranged from 10.04 ± 0.31 µg/g (WH-S2V) to 49.79 ± 0.48 µg/g (Don-1) and 37.48% (Don-4) to 58.58% (WNL-HS), respectively. Higher leaf phenolic or flavonoid content did not necessarily translate into a higher antioxidant activity. This suggests that other forms of antioxidants other than phenolics or flavonoids might be responsible free radical scavenging activity in roselle leaves. Nevertheless, very high free radical percentage inhibition observed in the roselle leaves makes it an excellent material for mitigation against the adverse effect of free radicals. Nine promising accessions with high free radical scavenging activity have been identified for further improvement.
2002
The experiment was conducted in Al Saniya Township (located 15km north of Al-Qadisiyah) in the Summer Season 2016, to study the Measurement of some medical substances in Rosella plant (Hibiscus sabdariffa L.) extract using HPLC technique under the influence of different treatments. A factorial experiment was applied according to complete randomized blocks design (R.C.B.D), with three replicated. Three factors included three cultivars of Roselle plant are (red, white and lined), and The second factor is salicylic acid in three Concentration (0, 25, 50 mg.L-1) divided into four sprayings and The third factor is seaweed extract in three concentration (0, 7.5, 15 ml.L-1) divided into four sprayings. The results showed that the superiority of the red variety was significantly higher than that of Quercetin, Anthocyanin and Delphinidin-3-glucoside in the sepales, and the salicylic acid spray treatment was 50 mg/l. The seaweed extract was also sprayed at 15 ml, In most active substances. Th...
Total Phenolic Content & Antioxidant Activity of Roselle (Hibiscus Sabdariffa l.) Calyces Extracts
2020
Total phenolic content and antioxidant activity were determined in Roselle (Hibiscus Sabdariffa L.) calyces extracts. Dried Roselle calyces were collected from Fenooj Region in Systan & Baluchestan province of Iran. Liquid extracts of dried Roselle (Hibiscus Sabdariffa L.) calyces obtained using water, ethanol (50%), methanol (50%), ethanol-methanol (50-50%, v/v) to evaluate selected phenolic compounds and antioxidant activity using the β-carotene bleaching method, free-radical scavengering by DPPH. The phenolic compounds contents ranged from 24.36 (in water ) to 44.43 (in ethanol-methanol) mg of gallic acid 100 g -1 of dried calyces , and the antioxidant activity ranged from 39.45 % (in water ) to 60.43 % ( in ethanol-methanol) .Free radical scavengering capacity of extracts by DPPH were obtained in range of 43.85%(in water) to 91.77% ( in ethanol-methanol).The antioxidant properties of Roselle calyces make the extracts ideal for use in foods as a natural extract, concentrate, or p...