SEM study of the effect of passive ultrasonic irrigation on the cleanliness of dentinal tubules in endodontic retreatment (original) (raw)

Influence of Ultrasonic Irrigation and Chloroform on Cleanliness of Dentinal Tubules During Endodontic Retreatment-An Invitro SEM Stud

ABSTRACT Background: Ultrasonic irrigation has been proved for its remarkable cleaning efficiency in the field of endodontics. But its role in endodontic re-treatment has been understated. There is not much data available to understand the effect of ultrasonic irrigation for the evaluation of cleanliness of dentinal tubules when it is used with or without chloroform, a gutta percha solvent during endodontic retreatment. Aim: To compare the influence of ultrasonic irrigation with syringe irrigation on cleanliness of dentinal tubules after gutta perch removal for endodontic retreatment with or without the use of chloroform a gutta percha solvent using scanning electron microscope (SEM). Materials and Methods: Freshly extracted 45 human mandibular premolar teeth for periodontal and orthodontic reasons were taken and were occlusally adjusted to a working length of 19 mm. The root canals of all teeth were prepared chemo mechanically to a master apical file size 40 and were divided in various groups. In Group 1 (n = 5; control group), the canals remained unfilled. In Groups 2 and 3 (n = 20 each), the canals were filled using lateral compaction with gutta-percha and AH plus sealer, removal of root fillings was undertaken after 2 weeks using Gates Glidden drills and H files without chloroform in Group 2 and with chloroform in group 3. The specimen of Group 2 and 3 were further divided into two subgroups I and II (n=10). In subgroup I, irrigation was done using side vented needles and sodium hypochlorite. In subgroup II irrigation was done using passive ultrasonic irrigation with sodium hypochlorite. Thereafter, the roots were split and the sections were observed under SEM. The number of occluded dentinal tubules /total number of dentinal tubules were calculated for the coronal, middle and apical third of each root half. Statistical analysis was performed using one-way ANOVA followed by Tukey’s test using standardized technique. Result: Results indicated that the cleanest dentinal tubules were found in the control group (Group 1 where the canals were unfilled) followed by the non chloroform group with ultrasonic irrigation (Group 3 subdivision II) followed by chloroform group with ultrasonic irrigation (Group 2 subdivision II), the non chloroform group with syringe irrigation (Group 3 subdivision I) and least cleanliness was found in the chloroform group with syringe irrigation (Group 2 subdivision I). Conclusion: Under the limitations of this study it could be concluded that both ultrasonic and syringe irrigation showed cleaner canals when chloroform was not used. Irrigation when done with ultrasonics leads to cleaner tubules than syringe irrigation. Hence, mechanical methods of retrieval in conjunction with use of passive ultrasonic irrigation should be a part of retreatment protocol. dr VINEET VINAYAK | dr VINEET VINAYAK|

Efficacy of passive ultrasonic irrigation in removing root filling material during endodontic retreatment

Revista de Odontologia da UNESP, 2016

Introduction The aim of endodontic retreatment is to remove the previous root filling materials completely to allow for the subsequent steps of cleaning, canal shaping and filling replacement. Objective To evaluate the efficacy of passive ultrasonic irrigation (PUI) in the removal of residual root filling material after removing the bulk of the root filling with nickel-titanium rotary instruments. Material and method Eighteen mesial isthmus-containing roots of human mandibular molars were prepared and filled. The root canal filling was removed with the ProTaper Retreatment system and K-files. The specimens were randomized into 2 groups (n = 9), both irrigated with 2.5% NaOCl and 17% EDTA. In the conventional group, an irrigation syringe was used. In the PUI group, the PUI technique was used. The roots were sectioned longitudinally and evaluated under stereomicroscopy. Student’s t test was used to compare groups and the general linear model was used to compare the apical, cervical, a...

Effect of Passive Ultrasonic Irrigation on the Removal of Root Canal Filling During Retreatment Procedures

Abstract The aim of this in vitro study was to evaluate the efficacy of passive ultrasonic irrigation used as adjunct during endodontic retreatment and its effect on the removal of obturation material from canal walls. Thirty-six extracted human single-rooted teeth were instrumented and obturated with gutta-percha and zinc oxide-eugenol- based sealer using the lateral condensation technique. Teeth were stored at 37ºC in a humid environment for four weeks. The removal of root canal filling was performed using ProTaper™ universal retreatment rotary files without solvent. Teeth were then divided into three groups of 12 specimens each based on the irrigation technique: group 1) syringe irrigation technique with 5.25% NaOCl solution applied as a final irrigation; group 2) passive ultrasonic irrigation technique using # 25 stainless steel files and ultrasonic activation for one minute; group 3) passive ultrasonic irrigation technique applied three times of one minute each. The teeth were ...

The efficacy of ultrasonic irrigation to remove artificially placed dentine debris from human root canals prepared using instruments of varying taper

International Endodontic Journal, 2005

van der Sluis LWM, Wu M-K, Wesselink PR. The efficacy of ultrasonic irrigation to remove artificially placed dentine debris from human root canals prepared using instruments of varying taper. International Endodontic Journal, 38, 764-768, 2005. Aim To investigate the influence of the taper of root canals on the effectiveness of ultrasonic irrigation to remove artificially placed dentine debris. Method Forty-four maxillary and mandibular canines were selected after bucco-lingual and mesiodistal radiographs indicated that their internal diameters were smaller than the diameters of a size 20, .06 taper System GT instrument (Dentsply Maillefer, Ballaigues, Switzerland). These canines were divided into three groups and prepared using either size 20, .06 taper System GT instruments, size 20, .08 taper or size 20, .10 taper System GT instruments. Each root was then split longitudinally through the canal, forming two halves. In one canal wall, a standard groove was cut 2-6 mm from the apex, to simulate uninstrumented canal extensions. Each groove was filled with dentine debris mixed with 2% NaOCl to simulate a situation when dentine debris accumulates in the uninstrumented canal extensions. Each canal was reassembled by joining the two halves of the teeth by means of wires and sticky wax. In each canal ultrasonic irrigation was performed with a size 15 K file using 2% NaOCl as an irrigant. Before and after irrigation, images of each half of the canal with a groove were taken using a microscope and a digital camera, after which they were scanned into a PC as TIFF images. The quantity of dentine debris in the groove was evaluated using a scoring system: the higher the score, the larger the amount of debris. The scores before and after irrigation were compared. The differences in percentage of score reduction between the three groups were analysed by means of one-way anova. Results After ultrasonic irrigation, the debris score reduced by 74, 81 and 93%, respectively, in the size 20, .06, 20, .08 and 20, .10 taper groups. However, the difference amongst groups was not statistically significant (P ¼ 0.078). Conclusion There was a tendency that ultrasonic irrigation was more effective in removing artificially placed dentine debris from simulated canal extensions from canals with greater tapers.

Analysis of the effect of ultrasonic agitation on the cleaning of root canals using different periods during the final irrigation

RSBO

The ultrasonic agitation was introduced as an adjuvant to conventional chemo-mechanical debridement during endodontic treatment to overcome the persistence of biofilms. Objective: To verify the cleaning of root canals irrigated with sodium hypochlorite (NaOCl) and ethylenediaminetetraacetic acid (EDTA), with or without an ultrasonic agitation, using different time periods and images obtained by scanning electron microscope (SEM). Material and methods: Forty mandibular incisors were cleaned, shaped and randomly divided into five groups according to the final irrigation protocol: SH10 group (ultrasonic agitation with NaOCl for 10 s), SH30 group (ultrasonic agitation with NaOCl for 30 s), SHE30 group (ultrasonic agitation with NaOCl and EDTA for 10 s), SHE90 group (ultrasonic agitation with NaOCl and EDTA for 30 s), and control group (NaOCl and EDTA without ultrasonic agitation). The teeth were prepared and analyzed by SEM at ×2000. The Kruskal–Wallis test was used with a 5% level of s...

Ultrastructural investigation of root canal dentine surface after application of active ultrasonic method

Srpski arhiv za celokupno lekarstvo, 2008

INTRODUCTION The basic work principle of all ultrasonic techniques is the piezoelectric effect of producing high frequency ultrasounds of small length, which are transmitted over the endodontic extensions or canal instruments into the root canal. When in contact with the tissue, ultrasonic vibrations are converted into mechanical oscillations. Ultrasonic waves and the obtained oscillations along with the synergic effect of irrigation bring about the elimination of smear layer from the root canal walls. OBJECTIVE The aim of the study was to ultrastucturally examine the effect of smear layer removal from the walls of canals by the application of the active ultrasonic method without irrigation, that is by the application of ultrasound and irrigation using distilled water and 2.5% NaOCl. METHOD The investigation comprised 35 single-canal, extracted human teeth. After removal of the root canal content, experimental samples were divided into three groups. According to the procedure requir...

A Scanning Electron Microscope (SEM) Based Evaluation of Effectiveness of Chloroquick, 7% Maleic Acid, and Conventional Multistep Irrigating Solutions with Passive Ultrasonics in Removal of Root Canal Smear Layer

Journal of Advances in Medicine and Medical Research

Aim: The aim of this study was to compare and evaluate the effectiveness of three different irrigation solutions, namely Chloroquick, maleic acid, and a multi-step irrigation solution when used in combination with ultrasonics for the removal of smear layers in endodontic procedures. Materials and Methods: Sixty single-rooted teeth that had been freshly extracted were gathered, disinfected, and stored in distilled water. To ensure uniform root length, each tooth was coronally removed below the Cementoenamel Junction (CEJ). The working length was meticulously determined, and F3 Protaper rotary files were employed for the biomechanical preparation of the root canals. Subsequently, the teeth were randomly assigned to one of three groups for the irrigation process: Group A (Chloroquick), Group B (Maleic Acid 7%), and Group C (Multistep Irrigation approach). Following the preparation, longitudinal sections of the specimens were obtained and examined under a scanning electron microscope. T...

Effectiveness of passive ultrasonic irrigation in improving elimination of smear layer and opening dentinal tubules

2014

Objective: To compare the ability to eliminate debris and open dentinal tubules of different ultrasound irrigation procedures. Study Design: Forty extracted single-rooted human teeth were instrumented with mechanical rotatory instrumentation, and divided into four groups: (n=10) according to the final irrigation technique: group A (control), 2.5% NaOCl irrigation with the Miraject needle and no ultrasonic agitation; group B, passive ultrasonic irrigation (PUI) with 2.5% NaOCl and Irrisafe 20 tips; group C, PUI with 2.5% NaOCl and Irrisafe 25 tips; group D, PUI with 2.5% NaOCl and K 25 tips. The amount of debris and the number of opened dentinal tubules was established by scanning electronic microscope. Data were compared using the Kruskal Wallis test. Results: Irrisafe tips (groups B and C) opened up more dentinal tubules and eliminated more debris than conventional irrigation (p<0.05) in the apical third. The middle third shows no significant differences between groups. Irrisafe 25 was more effective than conventional irrigation and K tips (p<0.05) in the coronal third. Conclusions: Ultrasonic activation of the irrigation with Irrisafe tips was the most effective procedure for eliminating the debris and opening up dentinal tubules, especially in the apical third.