Analysis of the effect of ultrasonic agitation on the cleaning of root canals using different periods during the final irrigation (original) (raw)

Effect of ultrasound streaming on the disinfection of flattened root canals prepared by rotary and reciprocating systems

Journal of applied oral science : revista FOB

New technical and scientific developments have been advocated to promote the success of the endodontic treatment. In addition to rotary and reciprocating systems, irrigating solution agitation has been suggested and passive ultrasonic irrigation (PUI) is the most used. To evaluate, in vitro, the effect of ultrasound streaming (US) in the disinfection of flattened root canal systems prepared by the ProTaper, BioRaCe and Reciproc systems, utilizing the microbiological culture. Extracted human mandibular incisors (n=84) were used. Suspensions of Enterococcus faecalis (ATCC 29212) were standardized and inserted along with the teeth immersed in brain-heart infusion (BHI) broth. The contamination was made following a protocol during 5 days. The teeth were randomly divided into six groups: G1, ProTaper Universal; G2, ProTaper Universal with US; G3, BioRaCe; G4, BioRaCe with US; G5, Reciproc; and G6, Reciproc with US. Irrigation was performed with saline solution. After biomechanical prepar...

Root canal debridement efficacy of heated sodium hypochlorite in conjunction with passive ultrasonic agitation: An ex vivo study

Journal of Dental Research, Dental Clinics, Dental Prospects

Background. This study aimed to investigate the endodontic debridement efficacy of different sodium hypochlorite (NaOCl) irrigation regimens with and without ultrasonic agitation, followed by ethylenediaminetetraacetic acid (EDTA) via scanning electron microscopy (SEM) after using a rotary instrumentation system. Methods. Mandibular premolars (n=50) were randomly divided into five experimental groups (n=10) for root canal instrumentation with ProTaper Universal rotary system up to F3. The root canal system was treated with intracanal-heated NaOCl (100°C) or preheated NaOCl (55°C), followed by ultrasonic agitation and EDTA treatment. Samples irrigated with conventional needle irrigation (CNI) using normal saline solution were used as controls. Debridement efficacy was analyzed by SEM. A five-point scale was used to estimate the presence/absence of debris for each canal segment (coronal, middle, and apical). The results were analyzed using one-way ANOVA and post hoc Tukey tests (P<...

Effect of the Passive Ultrasonic Irrigation and the Apical Diameter Size on the Debridement Efficacy of Infected Root Canals: A Multivariate Statistical Assessment of Histological Data

Applied Sciences, 2021

The removal of necrotic and vital pulp substrates and microorganisms and their toxins from the root canal system (RCS) has been found to be the basis for a successful endodontic treatment. In this study, our aim was to evaluate the effect of passive ultrasonic irrigation (PUI) on the elimination of the organic remnant tissue from infected, narrow and curved mandibular root canals during their instrumentation. For this purpose, mesiobuccal canals from mandibular first molars were instrumented with the RaCe rotary system, using PUI activation or conventional irrigation (CI) and two apical diameters (#25 and #35). The root canal cleanness of the samples was evaluated by microscopy and using a modified Langeland’s ordinal scale. Parametric and non-parametric statistical analyses and principal coordinates analysis (PCoA) of the samples were performed. When PUI was used, there was a significant reduction of the organic remnant in the apical enlargement of 25 at 2 mm from the apex (p < ...

A Scanning Electron Microscope (SEM) Based Evaluation of Effectiveness of Chloroquick, 7% Maleic Acid, and Conventional Multistep Irrigating Solutions with Passive Ultrasonics in Removal of Root Canal Smear Layer

Journal of Advances in Medicine and Medical Research

Aim: The aim of this study was to compare and evaluate the effectiveness of three different irrigation solutions, namely Chloroquick, maleic acid, and a multi-step irrigation solution when used in combination with ultrasonics for the removal of smear layers in endodontic procedures. Materials and Methods: Sixty single-rooted teeth that had been freshly extracted were gathered, disinfected, and stored in distilled water. To ensure uniform root length, each tooth was coronally removed below the Cementoenamel Junction (CEJ). The working length was meticulously determined, and F3 Protaper rotary files were employed for the biomechanical preparation of the root canals. Subsequently, the teeth were randomly assigned to one of three groups for the irrigation process: Group A (Chloroquick), Group B (Maleic Acid 7%), and Group C (Multistep Irrigation approach). Following the preparation, longitudinal sections of the specimens were obtained and examined under a scanning electron microscope. T...

Review of ultrasonic irrigation in endodontics: increasing action of irrigating solutions

Medicina Oral Patología Oral y Cirugia Bucal, 2012

Introduction: Effective irrigant delivery and agitation are prerequisites for successful endodontic treatment. Ultrasonic irrigation can be performed with or without simultaneous ultrasonic instrumentation. Existing literature reveals that ultrasonic irrigation may have a very positive effect on chemical, biological and physical debridement of the root canal system as investigated in many in vitro studies. Objective: The purpose of this review article was to summarize and discuss the available information concerning ultrasonic irrigation in endodontics. Methods: This article presents an overview of ultrasonic irrigation methods and their debridement efficacy. In this paper the relevant literature on passive ultrasonic irrigation is reviewed. Information from original scientific papers or reviews listed in MEDLINE and Cochrane were included in the review. Results: The use of ultrasound in the irrigation procedure results in improved canal cleanliness, better irrigant transfer to the canal system, soft tissue debridement, and removal of smear layer and bacteria. There are many in vitro studies, but there is a need to standardize protocols, and correlate the clinical efficacy of ultrasonic devices with improved treatment outcomes. Understanding the basis of ultrasonic irrigation is fundamental for clinicians and researchers to improve the design and use of ultrasonic irrigation.

In vitro evaluation of endodontic debris removal as obtained by rotary instrumentation coupled with ultrasonic irrigation

Australian Endodontic Journal, 2006

The cleaning capacity of rotary Ni-Ti instrumentation using Profile GT files, coupled with irrigation energised by ultrasound, was evaluated. Thirty-six human mandibular incisors were instrumented in vitro using the crown-down technique with the Profile GT system to 1 mm from the anatomical apex to a size 30.04 taper file. The instrumented teeth were then divided randomly into four groups where various final irrigations were used: Group I: 100 ml of 1% NaOCl with a Luer-Lok syringe (control group); Group II, Group III and Group IV: final irrigation with 100 ml of 1% NaOCl energised by ultrasound for 1, 3 and 5 min, respectively. After biomechanical preparation, the apical thirds of the roots were submitted to histological processing and examination. The specimens were analysed at 40× magnification and the images submitted to morphometric analysis with an integration grid. ANOVA showed a statistically significant difference (P < 0.01) between the irrigation techniques. Group I (Luer-Lok syringe) showed the highest percentage of debris (35.81 ± 4.49) and was statistically different from the other groups energised by ultrasound: Group II (27.28 ± 4.49), Group III (24.39 ± 5.72) and Group IV (18.46 ± 5.25). It was concluded that rotary instrumentation using Ni-Ti files associated with final irrigation of 1% NaOCl energised by ultrasound leads to better debris removal from the apical third of mesio-distally flattened root canal.

SEM study of the effect of passive ultrasonic irrigation on the cleanliness of dentinal tubules in endodontic retreatment

International journal of health sciences

Aim and Objective: Endodontic retreatment is indicated in the event of a failure of initial treatment of the root canal and of an initial treatment that looks to be correctable with retreatment. This in-vitro study was designed to assess the efficacy of passive ultrasonic irrigation in endodontic retreatment, by employing scanning electron microscopes, on the cleanliness of dentine tubules with and without solvent in curved root channels. Material and method: Sixty human maxillary molar teeth were treated endontically. The protaper rotary instrument was used in root canals in the crown down procedure under copious 3% NaOCl (2 ml) and Glyde irrigation. The saline solution (1 ml) was used for final rinsing. The 10 sample root surfaces of each group were evaluated for SEM pictures. Results: Amongst group I and II here were more open dentinal tubules that were statistically significant (p<0.05) in Group II without solvent group. Group IV demonstrated larger, statistically significant...

Influence of Ultrasonic Irrigation and Chloroform on Cleanliness of Dentinal Tubules During Endodontic Retreatment-An Invitro SEM Stud

ABSTRACT Background: Ultrasonic irrigation has been proved for its remarkable cleaning efficiency in the field of endodontics. But its role in endodontic re-treatment has been understated. There is not much data available to understand the effect of ultrasonic irrigation for the evaluation of cleanliness of dentinal tubules when it is used with or without chloroform, a gutta percha solvent during endodontic retreatment. Aim: To compare the influence of ultrasonic irrigation with syringe irrigation on cleanliness of dentinal tubules after gutta perch removal for endodontic retreatment with or without the use of chloroform a gutta percha solvent using scanning electron microscope (SEM). Materials and Methods: Freshly extracted 45 human mandibular premolar teeth for periodontal and orthodontic reasons were taken and were occlusally adjusted to a working length of 19 mm. The root canals of all teeth were prepared chemo mechanically to a master apical file size 40 and were divided in various groups. In Group 1 (n = 5; control group), the canals remained unfilled. In Groups 2 and 3 (n = 20 each), the canals were filled using lateral compaction with gutta-percha and AH plus sealer, removal of root fillings was undertaken after 2 weeks using Gates Glidden drills and H files without chloroform in Group 2 and with chloroform in group 3. The specimen of Group 2 and 3 were further divided into two subgroups I and II (n=10). In subgroup I, irrigation was done using side vented needles and sodium hypochlorite. In subgroup II irrigation was done using passive ultrasonic irrigation with sodium hypochlorite. Thereafter, the roots were split and the sections were observed under SEM. The number of occluded dentinal tubules /total number of dentinal tubules were calculated for the coronal, middle and apical third of each root half. Statistical analysis was performed using one-way ANOVA followed by Tukey’s test using standardized technique. Result: Results indicated that the cleanest dentinal tubules were found in the control group (Group 1 where the canals were unfilled) followed by the non chloroform group with ultrasonic irrigation (Group 3 subdivision II) followed by chloroform group with ultrasonic irrigation (Group 2 subdivision II), the non chloroform group with syringe irrigation (Group 3 subdivision I) and least cleanliness was found in the chloroform group with syringe irrigation (Group 2 subdivision I). Conclusion: Under the limitations of this study it could be concluded that both ultrasonic and syringe irrigation showed cleaner canals when chloroform was not used. Irrigation when done with ultrasonics leads to cleaner tubules than syringe irrigation. Hence, mechanical methods of retrieval in conjunction with use of passive ultrasonic irrigation should be a part of retreatment protocol. dr VINEET VINAYAK | dr VINEET VINAYAK|

Effectiveness of Root Canal Debris Removal Using Passive Ultrasound Irrigation with Chlorhexidine Digluconate or Sodium Hypochlorite Individually or in Combination as Irrigants

The Journal of Contemporary Dental Practice, 2008

Aim The aim of this in vitro study was to evaluate the cleaning capacity of different auxiliary chemical substances energized with ultrasound in radicular dentin using morphologic analysis. Methods and Materials Twenty-eight single-canal, mandibular incisors were prepared with the Hero 642 canal preparation system following the sequence: 25/12, 30/06, 35/02, and 40/02, 1 mm from the apex. The specimens were then divided into four groups of seven teeth. During biomechanical preparation the teeth were irrigated with 2 ml of distilled water between files. Each group of specimens (n=7) received a final irrigation with 100 ml of the following irrigants that were activated with ultrasound for 3 minutes: Group 1- distilled water; Group 2- 0.2% chlorhexidine digluconate (CHX); Group 3- 2.5% sodium hypochlorite (NaOCl); and Group 4-the filtrate obtained after combining 0.2% CHX and 2.5% NaOCl. The apical portions of the root canals from each group were then submitted to histological processi...

The efficacy of ultrasonic irrigation to remove artificially placed dentine debris from human root canals prepared using instruments of varying taper

International Endodontic Journal, 2005

van der Sluis LWM, Wu M-K, Wesselink PR. The efficacy of ultrasonic irrigation to remove artificially placed dentine debris from human root canals prepared using instruments of varying taper. International Endodontic Journal, 38, 764-768, 2005. Aim To investigate the influence of the taper of root canals on the effectiveness of ultrasonic irrigation to remove artificially placed dentine debris. Method Forty-four maxillary and mandibular canines were selected after bucco-lingual and mesiodistal radiographs indicated that their internal diameters were smaller than the diameters of a size 20, .06 taper System GT instrument (Dentsply Maillefer, Ballaigues, Switzerland). These canines were divided into three groups and prepared using either size 20, .06 taper System GT instruments, size 20, .08 taper or size 20, .10 taper System GT instruments. Each root was then split longitudinally through the canal, forming two halves. In one canal wall, a standard groove was cut 2-6 mm from the apex, to simulate uninstrumented canal extensions. Each groove was filled with dentine debris mixed with 2% NaOCl to simulate a situation when dentine debris accumulates in the uninstrumented canal extensions. Each canal was reassembled by joining the two halves of the teeth by means of wires and sticky wax. In each canal ultrasonic irrigation was performed with a size 15 K file using 2% NaOCl as an irrigant. Before and after irrigation, images of each half of the canal with a groove were taken using a microscope and a digital camera, after which they were scanned into a PC as TIFF images. The quantity of dentine debris in the groove was evaluated using a scoring system: the higher the score, the larger the amount of debris. The scores before and after irrigation were compared. The differences in percentage of score reduction between the three groups were analysed by means of one-way anova. Results After ultrasonic irrigation, the debris score reduced by 74, 81 and 93%, respectively, in the size 20, .06, 20, .08 and 20, .10 taper groups. However, the difference amongst groups was not statistically significant (P ¼ 0.078). Conclusion There was a tendency that ultrasonic irrigation was more effective in removing artificially placed dentine debris from simulated canal extensions from canals with greater tapers.