Mycoplasma excretion in reproductive male and female goats (original) (raw)
Related papers
2010
Contagious agalactia affects goats and is caused by several species of mycoplasma including Mycoplasma agalactiae and Mycoplasma mycoides subsp. capri (Mmc). Male goats, latently infected with M. agalactiae and Mmc, were identified at a dairy goat breeding artificial insemination centre. In three samplings, conducted over 1 year, ear swabs were assessed for both of the above organisms using culture and PCR techniques. Serological examination for antibodies against these organisms was performed at each time-point and conjunctival, nasal, rectal and preputial swabs were taken from a sub-sample of animals.
2004
A preliminary epidemiological survey was carried out to find out whether contagious agalactia of sheep and goats was present in herds in the Czech Republic or Jordan. A total of 99 animals were examined for the presence of Mycoplasma agalactiae antigen; there were 64 animals from the Czech Republic and 35 from Jordan. All Jordanian animals showed signs of clinical disease. Biological materials for examination included 353 swabs (133 from sheep and 220 from goats) collected from conjunctival, nasal and vaginal mucosae and the external ear canal. A monoclonal antibody-based sandwich ELISA was used to detect the antigen. The results were positive in 11 animals, ambiguous in 10 animals and negative in the rest of them (78). They confirmed our assumption that the Czech Republic is still free from contagious agalactia of sheep and goats but demonstrated that Jordanian herds of small ruminants are infected with Mycoplasma agalactiae. Sheep, goats, contagious agalactia, antigen, Mycoplasma ...
Isolation and Detection of Mycoplasma agalactiae from Semen Samples of Goats
Archives of Razi Institute, 2017
Contagious agalactia (CA) is a highly infectious disease of goats and sheep, and is a form of Mycoplasmosis, which is usually enzootic. Since Mycoplasma agalactiae (M. agalactiae) is the main cause of this disease in goats, the aim of this study was to isolate and detect M. agalactiae from semen of goat bucks. Thirty-nine semen samples were collected from goat bulks, and all samples were cultured in PPLO broth medium supplemented for M. agalaciae isolation. The bacteria DNAs were extracted from clinical samples and the PCR assay was applied to detect Mycoplasma genus and M. agalactiae species using specific primers, which amplified a 163bp fragment in 16SrRNA gene and a 375bp fragment in lipoprotein gene. The PCR evaluations were performed for both the clinical samples and the cultures. Out of the 39 samples, 29 (74.3%) of the cultures were shown positive and typical Mycoplasma colonies grew on PPLO agar, which could be considered as the diagnostic method. In addition, 38 (97.4%) sa...
Investigation of Mycoplasma agalactiae by molecular methods in goat bucks in West of Iran
The presence of asymptomatic carriers of Mycoplasma agalactiae in goat buck populations, and the internal presence of this microorganism in some of these animals, indicated that the ability of this micro-organism captive in contagious agalactiae to colonize in other locations such as the respiratory system or genitalia. This study was designed to detect the excretion Mycoplasma agalactiae in goat bucks reared in Iran, where the disease is considered endemic. A total of 196 samples which included semen (n=49), conjunctiva (n=49), nasal (n=49) and ear (n=49) of goat bucks were collected from July to September 2013. To avoid the introduction of goat bucks carrying Mycoplasma organisms sub clinically in samples were obtained from all candidate goat bucks for PCR, to detect the presence of Mycoplasma agalactiae. Of the samples tested, 31.12% were scored positive for Mycoplasma spp. and Mycoplasma agalactiae was detected in seventeen samples, conjunctival (6 positive sample), nasal (1 positive sample), ear (5 positive sample) and semen (5 positive sample). The results obtained in the present study confirm of Mycoplasma agalactiae in the semen, conjunctival, nasal and ear in goat bucks without clinical symptoms of contagious agalactiae infection. The presence of this agent in male goat is considered very risky in terms of reproductive disorders and contagious agalactiae outbreaks in endemic areas in Iran. This is the first report of Mycoplasma agalactiae in goat bucks without contagious agalactiae signs in Iran.
2014
Background: Contagious agalactia (C.A) is an infectious syndrome of sheep and goat characterized by mastitis, arthritis, keratoconjunctivitis and abortion. Mycoplasma agalectiae is the main cause of the disease in small ruminants. Objective: The aim of this study was to isolate and identify Mycoplasma mycoides cluster using PCR and culture from goats of Kerman city in Iran. Results: A total of 298 samples were collected from ear, eye and nose swab and milk secretion. All samples were cultured in PPLO broth and agar. After culture 56 samples (18.79%) were positive and showed fried egg colony on the agar media. At the same time the bacterial DNAs were extracted by Phenol-Chloroform method and the PCR assay was applied for detection of Mycoplasma genus in 163bp fragment of 16SrRNA gene, M.agalectiae in 375bp fragment of lipoprotein gene and M.mycoides cluster in 259bp fragment of 16SrRNA gene in all clinical samples. Out of 298 samples, 127 samples (42.6%) were positive for Mycoplasma-...
Detection of Mycoplasma agalactiae by Polymerase Chain Reaction in Jordanian Sheep and Goat Herds
Acta Veterinaria Brno, 2007
The aim of the study was to ascertain whether sheep and goats from selected Jordanian herds were infected with Mycoplasma agalactiae, the most common aetiological agent of contagious agalactia of sheep and goats. All examined animals showed clinical signs of disease at the time of sample collection. The group included 35 animals, 15 sheep and 20 goats. For microbiological examination, a total of 107 swabs were taken from conjunctival, nasal, vaginal or preputial mucosae and from the external auditory canal. Identification of the species isolated was carried out by a polymerase chain reaction. Of the 35 animals, 21 (4 sheep and 17 goats) tested positive for Mycoplasma agalactiae. These results confirmed our assumption that this mycoplasma species is present in Jordanian herds and, for the first time, provided evidence that contagious agalactia of sheep and goats occurs in Jordan. Mycoplasma agalactiae, sheep, goats, Jordan, polymearase chain reaction Contagious agalactia of sheep and goats is a serious infectious disease affecting small ruminants. It is characterised by arthritis, keratoconjunctivitis, pneumonia and, in females, mastitis and occasional abortion. It may be caused by any of the five mycoplasma species associated with this disease, namely, Mycoplasma agalactiae, Mycoplasma mycoides subsp. mycoides LC (large colony), Mycoplasma capricolum subsp. capricolum, Mycoplasma mycoides subsp. capri and Mycoplasma putrefaciens (Nicholas 1996; Sarris 1996; Bergonier et al. 1997; Bölske 1994). However, Mycoplasma agalactiae (M. agalactiae) is still regarded, particularly in sheep, as the "classical" aetiological agent of contagious agalactia (Bergonier et al. 1997). Mycoplasma putrefaciens is found only in goats. However, other animal species, such as cattle, camels or small wild ruminants, can also be infected (Perrin et al. 1994). Contagious agalactia is at present an important disease in countries with intensive small animal husbandry, such as the Mediterranean region and the Balkan Peninsula, Western
2018
Respiratory tract infections are the major cause of economic loss in goats. Mycoplasma agalactiae acts as an important etiological agent in mastitis, arthritis, kerato-conjuctivitis, pleuropneumonia and septicaemia syndrome (MAKePS). This paper reports a case study regarding the outbreak of respiratory disease associated with M. agalactiae in a herd of goats in Thrissur district, Kerala. The goats were presented with symptoms of respiratory distress, conjunctivitis, lethargy and reduced production. Nasal swabs were collected and dispensed in modified Hayfick’s broth without crystal violet. After incubation at 37°C for about 72 h., DNA was isolated from the broth and subjected to Mycoplasma genus specific PCR, which revealed amplicons of 280 bp confirming the genus. Also, the solid medium which was inoculated with filtered drops of incubated broth presented typical fried egg like colonies after five days of incubation and the isolates were identified as Mycoplasma by PCR. The species...
Ten goats were experimentally infected with a Mycoplasma identified by biomolecular methods as Mycoplasma mycoides subsp. capri, strain Irbid which was isolated from goats in an outbreak of contagious agalactia in north Jordan and defined as ‘unusual’, due to its serological characteristics. Two groups of goats infected by the endotracheal route and by aerosol, respectively, were placed in contact with a third group of naive animals. Six weeks after infection, some animals from both the infected and contact groups presented fever and nasal discharge, followed by severe respiratory signs and polyarthritis. Organs were taken from animals that died during the trial or those that were sacrificed at the end of the trial. The results of microbiological isolation and immunohistochemical tests conducted on the organs were compared after a description of the clinical picture and anatomopathological and histopathological signs.
Pesquisa Veterinária Brasileira
ABSTRACT: Mycoplasmosis is a disease that may cause severe economical losses in goat and sheep herds, and it is associated with mastitis, polyarthritis, agalactia, conjunctivitis, pneumonia and reproductive failure. The objective of this study was to determine the occurrence of Mycoplasma agalactiae in milk samples and investigate the main risk factors associated with infection in goats from farms of the state of Paraíba, Brazil. For Mycoplasma agalactiae diagnosis, 251 milk samples were submitted to DNA extraction using a commercially available kit, following the manufacturer’s instructions and Polymerase Chain Reaction (PCR) was performed. In addition, questionnaires were applied to identify the main risk factors associated with contagious agalactia. Out of the two hundred fifty-one samples analyzed, 50 (19.9%, I.C. 15.1-25.4%) were PCR positive for M. agalactiae. In the risk factors analysis, some associations were observed for the following variables: size of the herd (P<0.00...
Journal of Dairy Research, 2009
This study was designed to assess the possible effects of mycoplasmas on the quality of milk produced by goat herds in a contagious agalactia (CA) endemic area with absence of classical symptoms. Several factors related to milk quality (percentages of fat, total protein, lactose and total solids, standard plate counts (SPC) and presence of Staphylococcus aureus) were compared in mycoplasma-infected and non-infected herds. To define the CA status of 26 herds on the island of Lanzarote (Spain), where CA is endemic, 570 individual milk samples and 266 bulk tank milk (BTM) samples were microbiologically analysed for the presence of Mycoplasma spp. A herd was considered infected by mycoplasmas when at least a sample (individual or BTM) was positive. BTM samples were also used to determine milk quality parameters. Mycoplasma infection was confirmed in 13 herds. A total of 31, 10 and 11 strains of Mycoplasma mycoides subsp. mycoides LC (MmmLC), Mp. agalactiae and Mp. capricolum subsp. capr...