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PCR-based diagnosis of Helicobacter species in the gastric and oral samples of stray dogs
Comparative Clinical Pathology, 2012
Helicobacter spp. have been detected in different parts of gastrointestinal tract of dogs including the oral cavity, stomach, intestines and recently, hepatobilliary system. However, the transmission pathways of Helicobacter spp. have not been yet fully elucidated. Research in the last decade has proposed that oral-oral and fecal-oral transmissions, among others, may be a plausible route of this gastric infection. This study was carried out primarily to determine the existence of pylori and non-pylori Helicobacter spp. in the oral secretions and dental plaque of stray dogs of Iran as one of the possible routes of humans and animal infection and, secondly, to evaluate the accordance between oral and gastric colonization of Helicobacter spp. in these dogs. Forty-eight adult stray dogs were studied by PCR using 16S rRNA, Helicobacter felis, Helicobacter heilmannii, and Helicobacter pylori specific primers. Positive samples for 16S rRNA specific primers that did not meet the specified species of Helicobacter genus were randomly subjected to sequencing. Helicobacter spp. DNA was found in the oral and gastric specimens of 100 % of the stray dogs. There was not, however, any agreement between Helicobacter colonization at these two locations, at neither genus nor species level. Our study confirmed that the oral cavity of stray dogs routinely exposed to transient forms of bacteria and may temporarily harbor Helicobacter spp and Wolinella spp. Therefore, oral cavity as a source of Helicobacter spp. may act as a reservoir for transmission. However, it may not necessarily reflect the colonization status of the gastric mucosa.
Characterization of Helicobacter species isolated from humans and dogs in Sharkia Governorate, Egypt Mohamed, M. E. M.; Suelam, I. I. A. and Saleh, M. A. (1) Department of Zoonoses, Faculty of Veterinary Medicine, Zagazig University, Egypt. (2) Laboratory Animal Unit, Veterinary Hospital, Zagazig University, Egypt (3) Animal Health research Institute, Dokki, Giza, Egypt Abstract The present work was carried out to study the occurrence of Helicobacer species in humans and dogs in Sharkia Governorate and characterization by antibiogram and Polymerase Chain Reaction. A total number of 35 human gastric biopsy samples were collected endoscopy Unit, Department of Internal Medicine, Faculty of Medicine, Zagazig University and 30 samples were collected from stray dogs (20 male and 10 female). Both Helicobacter pylori and Helicobacter heilmannii occur in the stomach of examined human subjects with the frequencies of 40% (14/35) and 8.6% (3/35), respectively. Helicobacter pylori occurs in peptic ulcers and apparently healthy cases with the frequencies of 44% (11/25) and 30% (3/10), respectively, moreover, Helicobacter heilmannii occurs with the frequencies of 12% (3/25) and zero% (0/10), respectively. Helicobacter pylori and H. heilmannii occurs in the stomach of examined dogs with the frequencies of 6.7% (2/30) and 33.3% (10/30), respectively. H. pylori occurs in gastroenteritis and apparently healthy cases with the frequencies of 7.1% (1/14) and 6.3 %( 1/16), respectively. Nevertheless, H. heilmannii occurs with the frequencies of 42.9 (6/14) and 25 (4/16), respectively. Representative strains of Helicobacter species were confirmed by PCR technique. The used primers were Helicobacter genus-specific PCR for 16S rRNA gene. Concerning the antibiotic sensitivity test, it was found that multi-drug resistance phenomena are emerged in Helicobacter species. The public health significance of the isolated Helicobacter species was discussed. The obtained results reveal that dogs in Sharkia Governorate are potential reservoir for Helicobacterioses.
Veterinary Medicine International
Helicobacter pylori is a spiral-shaped bacterium, which plays a role in the aetiology of gastric diseases in humans. Non-H. pylori Helicobacter (NHPH) species naturally colonise the stomach of animals and also induce gastric lesions in humans, highlighting their zoonotic importance. We evaluated the gastric bacterial colonisation density and gastric lesions and sought to identify the main phylogenetic groups of the Helicobacter spp. obtained from dogs in the central region of Rio Grande do Sul, Brazil, with this study aiming to investigate the occurrence of Helicobacter spp. in saliva and gastric samples from these dogs. This study included 35 dogs and used analysis such as cytology, histopathology, PCR, rapid urease testing, and phylogenetic analysis. Of the dogs, 94.3% were positive for Helicobacter spp., and these bacteria were present in the stomach of 32 dogs and saliva of eight. Respectively, eight, 15, and nine dogs had mild, moderate, and severe colonisation. Lymphocytic-pla...
Epidemiologic effects on helicobacter spp. infections of dogs in Slovenia
Veterinarski glasnik, 2008
The aims of our study were to determinate the prevalence of helicobacter in a population of Slovenian dogs, to characterize the intensity of infection and to find out if the epidemiological parameters i. e. age, feeding regimen, gender, breed, location and indoor/outdoor living conditions have any influence on infection and the intensity of infection. A total of 185 randomly chosen dogs from all parts of Slovenia, at ages from 9 days to 15 years, of both genders and 44 different breeds, without any gastrointestinal disorders, were included in our study. Helicobacter were detected in stomachs of 92.4% of dogs. We determined a mild infection in 17.3% of dogs, a moderate infection in 48.1%, and a strong infection in 27% of dogs. Studying epidemiological parameters and their effects on infection we concluded that age and feeding regimen affect the infection and the intensity of infection, whereas gender, breed, location and indoor/ outdoor living conditions do not.
Helicobacter canis sp. nov., a new species from dogs: an integrated study of phenotype and genotype
Journal of general microbiology, 1993
A group of Campylobacter-like organisms (CLOs) were isolated from the faeces of diarrhoeic or healthy dogs, constituting 4% of all CLOs from this source. Since they formed a unique DNA homology group within the genus Helicobacter, and exhibited distinctive phenotypic properties, they were collectively termed the HC group. A polyphasic taxonomic analysis was made of this group. The phenotype of four dog isolates and a single human isolate was unique and could be distinguished bacteriologically from other helicobacters. Electron microscopic ultrastructure revealed defining characteristics of Helicobacter. The 16S rRNA gene of the nominated type strain NCTC 12739T was sequenced, and its analysis delineated the group as a new species of Helicobacter. This conclusion was supported by relative DNA homology and whole-cell protein electrophoretic patterns. We therefore propose the name Helicobacter canis sp. nov. for this group. The species most closely related to H. canis sp. nov. were H. ...
Occurrence and characterization of gastric Helicobacter spp. in naturally infected dogs
Veterinary Microbiology, 1999
Helicobacter-like organisms are frequently observed in the stomach of dogs but the relationship between these microorganisms and gastric pathology has not been clearly established. Different species of helicobacters are known to be present in the canine stomach but their specific prevalence in naturally infected dogs is unknown. The aims of this study were to isolate and characterize helicobacters in canine gastric biopsies, to compare the commonly used tests for the identification of Helicobacter spp. and to determine the occurrence of these species in dogs. Twenty-three out of 25 dogs (92%) were positive for Helicobacter-like organisms in cytological screening. Culture was successful from biopsies of 5/25 dogs. The isolates were analyzed by electron microscopy, biochemical and physiological tests, whole protein analysis and 16S rDNA sequencing. Helicobacter felis was identified in four samples and Helicobacter bizzozeronii in one sample. Only the whole protein analysis in combination with electron microscopy was able to clearly discriminate the two species. Compared to the high prevalence of Helicobacter-like organisms, the occurrence of H. felis and H. bizzozeronii, was low (17 and 4%, respectively). No Flexispira rappini-like organisms or H. salomonis were detected. Electron microscopy revealed that H. bizzozeronii-like microorganisms were present in three additional biopsies where we were unable to culture any Helicobacter-like organisms. These observations indicate that in the stomach of dogs not all helicobacters are culturable. The unculturable bacteria appeared to be the prevalent ones and may represent different spiral organisms. The presence of distinct helicobacters with different Veterinary Microbiology 70 (1999) 239±250 (J.G. Kusters) 0378-1135/99/$ ± see front matter # 1999 Elsevier Science B.V. All rights reserved. PII: S 0 3 7 8 -1 1 3 5 ( 9 9 ) 0 0 1 5 0 -9 characteristics can reflect different roles in the pathogenesis of canine gastric disease. #
Dog as Potential Source of Helicobacter pylori in Egypt: Public Health Significance
World Vet. J., 2020
Helicobacter species are a group of Gram-negative, microaerophilic bacteria, which are known to colonize the gastrointestinal and biliary tracts of humans and various animal species. The objective of the present study was to determine the prevalence of Helicobacter pylori in owned dogs and their role in the transmission of H. pylori to the dog owners. For this purpose, 60 gastric biopsy samples from dog owners and 80 stool samples from owned dogs were collected and examined for the presence of H. pylori 16s rRNA gene by nested PCR. The PCR positive samples from human and dog isolates were further subjected to partial Helicobacter genus-specific 16s rRNA gene sequencing. Phylogenetic analysis based on partial sequence of this gene was performed to determine the relationship between human and dog isolates. H. pylori was detected in 62.5% and 91.6% of dog and human samples, respectively. The nucleotide sequence of Helicobacter genus-specific 16s rRNA gene of human and dog isolates were similar. In conclusion, this study indicated a high prevalence of H. pylori in both dogs and dog owners in Egypt. Zoonotic transmission of H. pylori between dogs and humans is probable and represents a public health concern.