Fabrication of Microbicidal Silver Nanoparticles: Green Synthesis and Implications in the Containment of Bacterial Biofilm on Orthodontal Appliances (original) (raw)
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Green synthesis of silver nanoparticles: properties and action against bacterial biofilms
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Silver nanoparticles have gained the spotlight for their application in diverse fields over the last decade. Their properties range from optical, conduction, electrochemical and electromagnetic to biological properties like antioxidant, antimicrobial, anti-inflammatory, anti-tumor, and so on. Many investigations on silver nanoparticles show that they have the potential for eradication of antimicrobial resistance caused due to bacterial biofilms, which has become a setback against fighting microbial infections. Silver nanoparticles act against biofilms in multiple ways including damage to DNA, denaturation of proteins of the organism, creating pores or interfering with plasma membrane, generating reactive oxygen species, and many more mechanisms that make it difficult for a bacteria to gain resistance against these silver nanoparticles. Owing to their effective antimicrobial properties, their synthesis and properties are being explored and investigated. The most rational and sustainable way for the synthesis of silver nanoparticles is from plant extracts and microbes rather than chemical or physical synthesis which are high energy, temperature, machinery, labor, and cost requiring. Therefore, this review highlights the process of green synthesis for silver nanoparticles production, hinting at some of their important properties that make it unique and effective for applications in diverse felids as well as their action against bacterial biofilms as an upsurge against treating antibiotic resistance.
InTech eBooks, 2018
The porpoise of the study was to evaluate the antibacterial effect of silver nanoparticles (Ag-NPs) versus chlorhexidine (CHX) against Streptococcus mutans and Lactobacillus casei. Three different reducing agents were used for the synthesis and characterization of Ag-NPs: sodium borohydride (NaBH 4), a chemical method, and Heterotheca inuloides (Hi) and Camellia sinensis (Cs), two eco-friendly methods. The synthesized substance was deposited on deciduous teeth. Its behavior in dental tissues was evaluated through an energy dispersive X-ray spectroscopy (EDS) analysis, using a scanning electron microscope (SEM). The characterization of Ag-NPs in terms of shape, size, and polydispersity was performed through spectrophotometry of ultraviolet-visible light analysis (UV-vis), as well as by transmission electron microscopy. Isolation and culture of strains S. mutans and L. casei were done to perform the microbiological analysis. In Petri dishes, paper discs containing different concentrations of Ag-NPs (synthesized by Hi, and by Cs) were deposited and tested along with paper discs containing CHX. Their antibacterial effect against both bacteria was evaluated by the inhibition zones test. By means of UV-Vis and TEM analysis, it was possible to observe that Heterotheca inuloides produced smaller and more stable nanoparticles, also in greater quantities (17.5 nm), when compared to Camellia sinensis. EDS analysis through SEM showed a 6.25 average absorption of silver in dental tissues. The microbiological analysis revealed a greater zone of inhibition when the test bacteria were in contact with 20 μl of Ag-NPs, synthesized by Hi, being statistically significant (p < 0.05), compared to the growth inhibition zones produced by Cs, and CHX against both strains. We can conclude that eco-friendly methods produced Ag-NPs with an important antibacterial effect in both strains.
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HSOA Journal of Nanotechnology: Nanomedicine & Nanobiotechnology, 2019
Green synthesis is an ecological system for the production of eco-friendly and well characterized metallic nanoparticles using plants. In this research work, the synthesis of silver nanoparticles was from the extract of Annona muricata leaves. The characteri- zation of silver nanoparticles was done with Fourier Transform In- frared spectroscopy (FTIR), UV-vis spectrometry and transmission electron microscope to determine functional groups, shape, size and morphology of synthesized nanoparticle. The anti-antimicrobi- al potency of the synthesize nanoparticle was investigated via well diffusion method. The UV spectrum of synthesized nanoparticle re- vealed absorbance at 435.00nm which confirmed the formation of silver nanoparticle. The FTIR analysis shows bands corresponding to -OH, C=O and -NH2 nanoparticle are spherical shape of the func- tional group. The micrograph obtained from TEM analysis confirmed that the synthesized silver is spherical in shape. The antimicrobial investigation of the silver nanoparticles shows good antibacterial ac- tivity due to high zones of inhibition against test bacteria. This work revealed that the synthesized silver nanoparticles possess great an- timicrobial potency.
A Review on Antimicrobial Effect of Silver Nanoparticle against Biofilm Producing Bacteria
2019
In recent era multi drug resistant biofilm forming bacteria band Nanotechnology is a burning field for the researchers. Microbial infection caused by Biofilm forming bacteria due to ability of these bacteria to form Biofilm. Formation of biofilm is characteristic for various gram negative and gram positive bacteria hence Staphylococusaureus are most common biofilm producing bacteria. Biofilm composed by several layers of bacterial encased with in an exopolysachharidematrix. In this review it has been observed that Nanotechnology play a key role in control and prevention of the infection occur due to multidrug resistant biofilm forming bacteria.In most of the observation antimicrobial effect of silver nanoparticle it has been determine by disc diffusion and microdilution technique. For the determination of biofilm production Congo redagar technique and Tube technique has been used. Due to the significance of biofilm in medical science and increase of drug resistance, silvernanopartic...
Development of biologically inspired green synthesis of silver nanoparticles is evolving into an important branch of nano-biotechnology. In the present investigation, we report the green synthesis of silver nanoparticles (AgNPs) employing the leaf extract of Justicia glauca. Water-soluble organics present in the leaf extract are mainly responsible for the reduction of silver nitrate (AgNO3) solution to AgNPs. The AgNPs are 10-20nm in dimensions as determined by TEM images. The antimicrobial activities of green synthesized AgNPs and drug blended AgNPs have been evaluated against the dental caries and periodontal disease causing microorganisms such as Streptococcus mutans, Staphylococcus aureus, Lactobacillus acidophilus, Micrococcus luteus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and Candida albicans. The AgNPs and drug blended AgNPs show a significant antibacterial and antifungal activity. Minimum inhibitory concentration (MIC) value of AgNPs determined against the selected dental caries and periodontal disease causing microorganisms are noticeable between the range of 25-75μg/mL.