Acid stress suggests different determinants for polystyrene and HeLa cell adhesion in Lactobacillus casei (original) (raw)

Evaluation of adhesion properties of lactobacilli probiotic candidates

Monatshefte für Chemie - Chemical Monthly

Bacterial adhesion is a complex phenomenon implicated in the host-bacterial interaction that is pivotal for probiotic activity. Eight probiotic lactobacilli candidates (Lactobacillus reuteri, L. plantarum, L. mucosae, L. murinus) were screened for their ability to adhere to abiotic and biotic surfaces in vitro. Adhesion to hydrocarbons was used for hydrophobicity assessment. Three strains of L. reuteri and L. murinus C were evaluated as hydrophobic, others as intermediate. All tested strains were able to form the biofilm on polystyrene. L. mucosae D and L. reuteri E were tested for adhesion to epithelial cell lines (HeLa and Caco-2). Both were more adherent to HeLa than to Caco-2. The adhesivity degree in HeLa reached the highest value after 8 h of co-cultivation in both lactobacilli tested, then decreased. In Caco-2, adhesion was increased within 24 h from the beginning of the co-cultivation. Mucus-binding protein gene, implicated in adhesion, was detected in L. mucosae D. Therefore, the involvement of proteinaceous substances in binding process was investigated. Cells of L. mucosae D were digested by three proteolytic enzymes (proteinase K, pronase E, trypsin) and evaluated for time-dependent adhesivity changes to HeLa, Caco-2, and L929 cell lines. Results confirmed that proteins are most likely to play an important role in binding of lactobacilli to eukaryotic cells. One hour after treatment, L. mucosae D was able to overcome the effect of proteolytic cleavage. We assume that it was due to the restoration of its cell-surface binding structures. Co-cultivation of HeLa and L. mucosae D led to protuberance and communication channels formation in eukaryotic cells.

Adhesive properties of food and faecal potential probiotic lactobacilli

Journal of Applied and Natural Science

In the present investigation, total four isolates of Lactobacillus species i.e. L. casei, L. helveticus, L. brevis and L. fermentum were examined for the cell surface hydrophobicity by bacterial adherence to hydrocarbons assay in LAPTg broth and hydrophobicity was calculated as percentage decrease in Optical Density at 600 nm. The general range of hydrophobicity in Lactobacilli was found in between 6-73%. Remarkably, L. helveticus and L . fermentum showed 73% hydrophobicity in xylene. Higher value of hydrophobicity could point toward a better ability of lactobacilli to adhere to epithelium cells. The outcome of present study concludes that L. helveticus and L. fermentum have good adhesive properties which may help them to adhere to surface epithelium of host cell and further screening with other probiotic attributes could be designated as probiotics.

EVALUATION OF ADHESIVE PROPERTIES OF PRESUMPTIVE PROBIOTIC Lactobacillus plantarum STRAINS

Bioscience Journal

Thirty-two strains of Lactobacillus plantarum UFLA SAU from pork sausages, pre-selected for some features for probiotic application, were utilized in this study to evaluate their adhesive properties and compare the results against the three pathogens also tested. Strains were tested for autoaggregation and coaggregation capacity and Microbial Adhesion To Solvents (MATS) at the time intervals of 0, 1, 2, 3 and 4 h. Our findings revealed that UFLA SAU strains have a high autoaggregative capacity and coaggregative ability with pathogens, especially Listeria monocytogenes. In relation to adhesion to solvents, in general, L. plantarum strains showed hydrophilic cell surface properties and an important electron donor and basic character. Adhesive properties were markedly separated for the strains under study by Principal Component Analysis software. UFLA SAU 132, 226 and 87 were differentiated by autoaggregation ability. UFLA SAU 11 and Listeria monocytogenes were characterized by adhesion to solvents. UFLA SAU 14, 18 and 172 showed high coaggregation with Escherichia coli, Salmonella Typhi and Listeria monocytogenes. In comparison to the pathogens tested, many UFLA SAU strains presented higher adhesive capacity. These tests should be used for screening and identifying potentially adherent microorganisms. Adhesive properties are important features for the choice of probiotic strains and confer various applications, such as in the pharmaceutical (therapeutic or prophylactic) and food (functional foods) industries.

Adhesion mechanisms of lactic acid bacteria: conventional and novel approaches for testing

World Journal of Microbiology and Biotechnology, 2019

Adhesion ability is a primary criterion for the selection of probiotic microorganisms. Lactic acid bacteria contribute the majority of microorganisms with probiotic properties. They have several important mechanisms for intestinal epithelial cell adhesion. In order to adhere to the intestinal cells, they generally use various structures such as flagella, pili, S layer proteins, lipoteichoic acid, exopolysaccharides and mucus binding proteins. Various in vitro experiments were designed or study models were developed to reveal the mechanisms they utilize for binding to the intestinal cells, yet, the mechanisms for their adhesion are not fully explained. The major disadvantage of conventional models is the lack of layers forming the intestinal mucosa. Besides, these models omit the presence of natural microbiota, digestive conditions and the presence of a food matrix. Because of the disadvantages of existing models, natural tissues or novel applications like 3D organ cultures, which are better able to mimic in vivo conditions, are preferred.

Study of the adhesion of Lactobacillus acidophilus strains with probiotic properties to MDCK

Study of the adhesion of Lactobacillus acidophilus strains with probiotic properties to MDCK. Probiotic strains are required to have the ability to adhere to epithelial cells or cell lines. The presence of S-layer proteins in three Lactobacillus acidophilus strains with probiotic properties was examined. Their ability to adhere to the epithelial monolayer model non-cancerous cell line MDCK was studied as well. The strains Lactobacillus acidophilus A2, Lactobacillus acidophilus Ac and Lactobacillus acidophilus Z10 possess S-layer proteins and adhere to the cells of MDCK. Along with their other probiotic properties these make them suitable for inclusion in the composition of probiotics and probiotic foods. INTRODUCTION Probiotics are live microorganisms that have beneficial effects on the health of the host, when administered in adequate amounts [1, 11]. Some strains of the genera Lactobacillus, Bifidobacterium and some representatives of Propionibacterium are currently included in th...

Adhesion and aggregation properties of probiotic and pathogen strains

European Food Research and …, 2008

Autoaggregation has been correlated with adhesion, which is known to be a prerequisite for colonization and infection of the gastrointestinal tract by many pathogens. The coaggregation properties of probiotic strains with pathogens as well as their ability to displace pathogens are of importance for therapeutic manipulation of the aberrant intestinal microbiota. Consequently, the ability to aggregate and coaggregate are desirable properties for probiotics in health-promoting foods. Aggregation assays and bacterial adhesion to hydrocarbons (BATH test) demonstrated sig-niWcant diVerences in cell surface properties among the tested commercial probiotic strains. Hydrophobicity increased when the cells were heat-inactivated. All probiotic strains tested showed aggregation abilities with the pathogen strains tested, but the results were strain-speciWc and dependent on time and incubation conditions. Our results indicate that the ability to autoaggregate, together with cell-surface hydrophobicity and coaggregation abilities with pathogen strains can be used for preliminary screening in order to identify potentially probiotic bacteria suitable for human or animal use.

Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92

2003

B. K OS, J. SUSKOVIC ´ ,S. V U K O V I C´ ,M. SIMPRAGA, J. F RECE A ND S. M ATOSIC ´ . 2003. Aims: To investigate aggregation and adhesiveness of Lactobacillus acidophilus M92 to porcine ileal epithelial cells in vitro, and the influence of cell surface proteins on autoaggregation and adhesiveness of this strain. Methods and

Fish Probiotics: Cell Surface Properties of Fish Intestinal Lactobacilli and Escherichia coli

Microorganisms

The properties of intestinal bacteria/probiotics, such as cell surface hydrophobicity (CSH), auto-aggregation, and biofilm formation ability, play an important role in shaping the relationship between the bacteria and the host. The current study aimed to investigate the cell surface properties of fish intestinal bacteria and probiotics. Microbial adhesion to hydrocarbons was tested according to Kos and coauthors. The aggregation abilities of the investigated strains were studied as described by Collado and coauthors. The ability of bacterial isolates to form a biofilm was determined by performing a qualitative analysis using crystal violet staining based on the attachment of bacteria to polystyrene. These studies prove that bacterial cell surface hydrophobicity (CSH) is associated with the growth medium, and the effect of the growth medium on CSH is species-specific and likely also strain-specific. Isolates of intestinal lactobacilli from fish (Salmo ischchan) differed from isolates...