PCR-based survey of vector-borne pathogens in dogs in Dhaka, Bangladesh (original) (raw)
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Korean Journal of Veterinary Research
The study of canine vector-borne diseases in the Philippines started in the 1970s but only gained interest in the past decade. Characterization of such diseases in the Philippines remains incomplete, thus, it is necessary to obtain additional information on the prevalence and diversity of canine tick-borne diseases in the country. In this study, blood samples were obtained at two veterinary clinics in Metro Manila, Philippines from 114 dogs suspected of having canine tick-borne pathogens. Polymerase chain reaction (PCR) was performed on whole blood DNA extracts followed by sequencing, and the following pathogens were detected: Hepatozoon (H.) canis (5.26%), Babesia (B.) vogeli (5.26%), Ehrlichia (E.) canis (4.39%), and Anaplasma platys (3.51%). Additionally, a set of multiplex PCR primers were developed to detect H. canis, Babesia spp. (B. canis and B. vogeli), and E. canis in canine blood. Multiplex and conventional single-reaction PCR results for the 114 dog blood samples were similar, except for one H. canis sample. Multiplex PCR is, therefore, a useful tool in screening infected dogs in veterinary clinics. This study's results, together with those of previous studies in the country, show that canine vector-borne pathogens are an emerging veterinary concern in the Philippines.
The incidence of tick-borne blood disease in domestic dogs was determined using polymerase chain reaction (PCR) technology. A total of 280 left-over blood samples from domestic dogs were obtained from animal hospitals in Khon Kaen, Thailand, from May 2012-April 2013. Most dogs for which clinical data were available, exhibited loss of appetite and slight fever, but some appeared to be in normal condition and had no history of tick infection. All blood samples were negative for parasites when detected by Giemsa staining and microscopic examination. Eighty (28.57%) out of 280 samples were positive for tickborne pathogen DNA. The most parasite that we detected was Babesia canis (48.75% of infected samples), followed by Ehrlichia canis (33.75%) and mixed infections of B. canis and E. canis (17.5%). All of the samples were negative to Hepatozoon canis. The present results indicate that PCR-based technology has high sensitivity and specificity for diagnosis of B. canis and E. canis. On the other hand, diagnosis of tick-borne pathogens by microscopic examination is not very sensitive, especially in cases of low infection or early stage of infection. Therefore, to ensure the accuracy of the results and to avoid misdiagnosis and incorrect treatment, detection at the molecular level may need to develop for diagnosis in animal clinics or animal hospitals.
Pathogens
Canine tick-borne pathogens (CTBPs) such as Babesia vogeli, Ehrlichia canis, Anaplasma platys, Hepatozoon canis, and Mycoplasma haemocanis are important pathogens in dogs worldwide. Rhipicephalus sanguineus sensu lato, the main vector of several CTBPs, is the most common tick species found on dogs in Thailand. The present study identified CTBPs in dogs and ticks infested dogs. Samples (360 dog blood samples and 85 individual ticks) were collected from stray dogs residing in 37 temples from 24 districts in Bangkok and screened for CTBPs using molecular techniques. The most common CTBP found infecting dogs in this study was Ehrlichia canis (38.3%) followed by Mycoplasma haemocanis (34.2%), Hepatozoon canis (19.7%), Babesia vogeli (18.1%), and Anaplasma platys (13.9%), respectively. Furthermore, A. platys (22.4%) was the most common CTBP in ticks followed by M. haemocanis (18.8%), B. vogeli (9.4%), H. canis (5.9%), and E. canis (2.4%), respectively. The detection of CTBPs from the pres...
Veterinary World, 2022
Background and Aim: Canine tick-borne pathogens (CTBPs) are an important cause of morbidity in dogs in Thailand. This study aimed to evaluate the occurrence of three CTBPs in clinically normal, owned dogs to understand the risk for the general canine population. We also examined sex, age, tick infestation, and packed cell volume (PCV) of the animals in association with active infection of the CTBPs. Materials and Methods: A total of 139 dogs were included in the study. Blood samples were collected for thin blood smear, PCV and nested polymerase chain reaction (PCR) assay. Statistical analyses were performed to examine the association between individual factors and CTBP infection status determined by PCR. In addition, sensitivity, specificity, and Cohen's kappa were calculated to assess the utility of routine blood smear. Results: The PCR results showed that 31 dogs (22.3%) were infected with at least one of the three pathogens. The occurrence rate for Ehrlichia canis, Anaplasma platys, and Hepatozoon canis was 2.2% (3/139), 18.7% (24/139), and 2.8% (4/139), respectively. There were two cases of coinfection with A. platys and E. canis. The univariate analyses did not yield any associations between recorded variables and the active infection. Microscopic examination showed good sensitivity and agreement only for H. canis (Sn: 75%, 95% confidence interval: 24.9-98.7, κ=0.85). Conclusion: Our findings confirmed the endemicity of the CTBPs in owned canine population in the study site. In-depth epidemiological investigation would be warranted to elucidate environmental risk factors for CTBP infection.
Canine tick-borne diseases in pet dogs from Romania
Parasites & Vectors, 2017
Background: Tick-borne diseases are of substantial concern worldwide for animals as well as humans. Dogs have been a human companion for millennia, and their significant impact on human life renders disease in dogs to be of great concern. Tick-borne diseases in dogs represent a substantial diagnostic challenge for veterinarians in that clinical signs are often diffuse and overlapping. In addition, co-infections with two or more pathogens enhance this problem further. Molecular methods are useful to disentangle co-infections and to accurately describe prevalence and geographical distribution of tick-borne diseases. At this point, this information is lacking in many areas worldwide. Romania is one such area, where prevalence and distribution of several important pathogens need to be further investigated. To address this, we screened blood samples from 96 sick dogs with molecular methods for eight different pathogens including Babesia spp., Theileria spp., Hepatozoon spp., Anaplasma spp., Ehrlichia spp., "Candidatus Neoehrlichia mikurensis", Mycoplasma spp., and Borrelia spp. Results: As many as 45% (43/96) of the dogs in the study were infected with protozoan parasites. Babesia canis was the most frequent of these (28 infected dogs), whereas Hepatozoon canis was detected in 15% (14/96) and Babesia gibsoni was found in a single sample. Bacterial infection with Mycoplasma spp. occurred in 18% (17/96) of the sampled dogs. Obtained bacterial sequences revealed the occurrence of two species: Mycoplasma canis and "Candidatus Mycoplasma haematoparvum". In several cases co-infection with protozoan parasites and Mycoplasma sp. were detected. All dogs were negative for Anaplasma spp., Ehrlichia spp., "Ca. Neoehrlichia mikurensis", and for Borrelia spp. Conclusions: The results from the present study reinforce the notion that Babesia canis is an important pathogen in the Romanian dog population. However, more surprisingly, another protozoan species, H. canis, seems to be infecting dogs to a larger extent than previously recognized in Romania. Well-known tick-borne bacterial disease agents such as Anaplasma spp. and Borrelia spp. were not detected. In contrast, less wellstudied bacteria such as hemotropic Mycoplasma spp. were detected frequently. Moreover, co-infection might aggravate disease and complicate diagnosis and should be further studied in dogs.
Frontiers in veterinary science, 2024
Tick-borne pathogens are transmitted by a wide range of tick species and affect both human and animal health. Understanding the diversity of these pathogens and their co-infection rates in domesticated animals in urban areas is crucial for effective disease management and prevention. In this study, a total of 565 owned dogs in the central region of Thailand were investigated for the infection rate of three genera of Ehrlichia, Hepatozoon, and Babesia infection using multiplex PCR. The results revealed an overall infection rate of 19.1%, with Ehrlichia having the highest infection rate (12.2%), followed by Babesia (2.5%) and Hepatozoon (1.4%). The rate of co-infection was 3%, with mixed infections involving two or three genera. Male dogs exhibited a slightly higher infection rate compared to females, although not statistically significant. Young adult dogs (1-3 years) showed the highest infection rate of both single infections and co-infections. Monthly infection rate indicated variations throughout the year, with co-infection rate significantly associated with overall infection rate. Clinical manifestations in three genera of infected dogs included thrombocytopenia and eosinopenia. The results of this study are useful to design strategies for the management and prevention of tickborne diseases in the study area.
A survey of canine tick-borne diseases in India
Background: There are few published reports on canine Babesia, Ehrlichia, Anaplasma, Hepatozoon and haemotropic Mycoplasma infections in India and most describe clinical disease in individual dogs, diagnosed by morphological observation of the microorganisms in stained blood smears. This study investigated the occurrence and distribution of canine tick-borne disease (TBD) pathogens using a combination of conventional and molecular diagnostic techniques in four cities in India.
Prevalence of tick-borne pathogens in domestic dogs in North-Eastern region of India
Biological Rhythm Research, 2018
In order to detect the prevalence of tick-borne pathogens in dogs, a total of 525 dog blood samples were examined by peripheral blood examination during different seasons. The survey was carried out from July, 2017 to June, 2018 in different parts of NorthEastern (NE) regions of India. Dogs were also closely inspected on their body surface for presence of ticks, if any. Out of 525 dog blood samples examined, 12.19% (64/525) were found positive for at least one type of tick transmitted pathogens as revealed by microscopic examination of stained blood smears. Twenty-eight dogs (5.33%) screened positive for Ehrlichia canis, eleven (2.09%) for Anaplasma platys, nine (1.71%) for Babesia gibsoni, eight (1.52%) for Babesia canis and eight (1.52%) for Hepatozoon canis. Dogs above two years age group showed higher rate of infection than dogs either between 1 and 2 years or below 1 year age group. Infection was higher during rainy season. Male dogs showed a higher proportion of infection than females. The only tick found during the study period was Rhipicephalus sanguineus. The results clearly indicate that vector-borne haemoprotozoa and rickettsia are prevalent in the study area in apparently healthy dogs without showing any obvious symptoms.
First molecular detection of tick-borne pathogens in dogs from Jiangxi, China
The Journal of veterinary medical science, 2016
In this study, blood samples obtained from 162 dogs in Jiangxi, China, were employed in molecular screening of canine tick-borne pathogens by PCR and sequencing. Babesia spp. gene fragment was detected in 12 (7.41%) dogs. All samples were negative for Hepatozoon spp., Ehrlichia canis, Coxiella spp., Borrelia spp., Rickettsia spp. and Anaplasma platys. Species-specific PCR analysis further confirmed that 8 (4.94%) and 4 (2.47%) dogs were infected by Babesia canis vogeli and Babesia gibsoni, respectively. Based on our analyses, Babesia spp. infection in Jiangxi appeared not related to age, gender, breed, usage, activity and health status or tick infestation history of the dogs. This is the first molecular report of Babesia canis vogeli and Babesia gibsoni in dogs from Jiangxi, China.
Molecular identification of Babesia spp. in naturally infected dogs of Kerala, South India
Journal of Parasitic Diseases, 2016
Canine babesiosis is a tick borne haemoprotozoan disease caused by large and small intraerythrocytic apicomplexan piroplasms of the genus Babesia spp. The clinical manifestations of the disease vary from inapparent subclinical form to hyperacute shock related haemolytic crisis. Microscopic examination of blood smears from suspected dogs revealed Babesia canis and B. gibsoni in 3.45 and 25.86 % of samples respectively. A seminested PCR based on previously published species specific primers targeting the 18S rRNA gene was utilized to identify the Babesia species infecting dogs of Kerala at the sub species level. The study revealed 57.5 % prevalence of Babesia spp. among dogs. This report also presents the first molecular evidence of Babesia canis vogeli and B. gibsoni among naturally infected dogs in Kerala, South India. Molecular survey revealed a high prevalence of B. gibsoni infections when compared with B. canis vogeli infections among canines of the state. Preliminary survey of the tick population revealed the presence of Rhipicephalus sanguineus, R. haemaphysaloides and Haemaphysalis bispinosa in infected animals. Further studies need be directed towards utilizing the PCR protocol for confirming the vectors of these species in the region.