Antibacterial activity of the chemical constituents from Ranunculus laetus (original) (raw)

Antimicrobial activity of five constituents isolated from Ranunculus muricatus

Journal of medicinal plant research

Antimicrobial activity of five compounds isolated from Ranunculus muricatus, namely, Tricin7-O-β-Dlucopyranoside (1), protocatechuyl aldehide (2), isoscopoletin (3), anemonin (4), Β-sitoseterol (5) were tested against two human Gram-positive bacteria (Staphylococcus aureus, Micrococcus luteus) and four Gram-negative ones (Escherichia coli, Pseudomonas aeruginosa, Enterobacter cloacae, Klebsiella Pneumoniae). All the five compounds isolated, were tested against the fungal strain Aspergilus niger. The structures of the compounds have been confirmed by EIS, HR-EIMS and 1 H-13 C-NMR spectroscopic methods. The antimicrobial activities were performed by agar well diffusion method. MIC and MBC were carried out by agar dilution method and viable cell count method, respectively. Compound 1 showed maximum antimicrobial activities while the other compounds also showed superior antimicrobial activities. These compounds were isolated first time from R. muricatus which are persuasive as standard antimicrobial drugs.

Antimicrobial and Synergistic Studies of Ranunculus Muricatus L. Against Some Indigenous Bacteria

2014

In the present study, antibacterial activity of the whole plant methanolic extract of Ranunculus muricatus L., was analyzed against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Bacillus pumilus, Salmonella typhimurium and Pseudomonas aeroginosa. Methanol was regarded as an excellent solvent for antimicrobial activity. It was observed as best bactericidal at a minimal inhibitory concentration (MIC) of 1-10 µg/ml against all the bacterial cultures viz. B. pumilus, B. subtilis, S. aureus, E. coli, P. aeroginosa and S. typhimurium. Synergistic antibacterial activity of methanolic extracts was tested with respect to solvent extract of leaves of Ricinus communis, Nerium oleander, Withania somnifera, whole plant of Heliotropiaum curassavicum and fruits of Citrullus colocynthis. Synergistical study revealed the best antibacterial activity against B. subtilis and B. pumilus at a level of 1 µg/ml except E. coli and S. aureus.

In vitro Antimicrobial Activity of the Chemical Ingredients of Ranunculus hirtellus

The phytochemical study on the n-butanol fraction of Ranunculus hirtellus led to the first time isolation of four known constituents (1-4).The antimicrobial and enzyme inhibition activities of these four constituents isolated from Ranunculus hirtellus are presented in this study. These constituents were screened against two human Gram-positive bacteria (Staphylococcus aureus, Micrococcus luteus) and four Gram-negative ones (Escherichia coli, Pseudomonas aeruginosa, Enterobacter cloacae, Klebsiella Pneumoniae) by using Agar well diffusion method for both antibacterial and antifungal activities. MIC was determined by Agar well dilution method. MBC was performed by viable cell count method. Compound (1) showed maximum antimicrobial activity while the other compounds showed least antimicrobial activities. In addition these four isolated compounds were screened for their Acetyl cholinesterase (AChE) and Butyryl cholinesterase (BChE) inhibitory activities. Moreover, the IC (50% inhibitory...

In vitro Screening of Ranunculus muricatus for Potential Cytotoxic and Antimicrobial Activities

2014

Antibacterial and antifungal activities of four fractions (n-hexane, chloroform, ethyl acetate and ethanol) ofRanunculus muricatus were tested against two human Gram positive (Staphylococcus aureus, Micrococcus luteus) and four Gram negative pathogen (Escherichia coli, Pseudomonas aeruginosa, Enterobactercloacae, KlebsiellaPneumoniae). The antimicrobial activities were performed by agar well diffusion method. MIC and MBC were carried out by agar dilution method and viable cell count method, respectively. Cytotoxic activities of the crude and fractions were determined by using brine-shrimp (Artemiasalina) lethality bioassay by Mayer. The Ampicillin, Ofloxacine and Itraconazole were used as standard agents. Nearly all fractions exhibited more or less antimicrobial activity. The Ethyl acetate fraction illustrated maximum antimicrobial activity, MIC was 0.119µg/ml against Staphylococcus areous. Likewise n-hexane fraction exhibited highestantifungal activity against Aspergillusniger whil...

Ranunculus Sericeus Banks & Sol. Extract Fractions Possess Antibacterial and Antifungal Activity

2018

Ranunculus sericeus, collected from Agri, was successively extracted with n-hexane, chloroform, acetone and methanol, in a Soxhlet extractor. Obtained extracts were tested for antibacterial activity against human and plant pathogenic bacteria Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus, Enterococcus faecalis, Xanthomonas axonopodis pv. vitians, Enterobacter cloacae, Burkholderia sepasia, Pantoea ananatis, and for antifungal activity against fungi Rhizoctonia sp ., Alternaria sp., and Fusarium sp. Minimum Inhibitory Concentration (MIC) values were determined. Our results indicate that acetone and methanol fractions have moderate antibacterial and antifungal activity.

Screening of Ranunculus sceleratus for enzyme inhibition, antibacterial and antioxidant activities

Bangladesh Journal of Pharmacology, 2015

Enzyme inhibition potential of various fractions of Ranunculus sceleratus was checked against α-glucosidase, butyrylcholinesterase, acetylcholinesterase and lipoxygenase enzymes. n-Butanol fraction showed very good activity (77.5 ± 1.0% inhibition at 0.1 mg/mL) against α-glucosidase. Its IC50 value was 35.7 ± 1.0 µg/mL comparable to quercetin (IC50 value 16.5 ± 0.4 µg/mL). Antibacterial activity was checked against five bacterial strains by 96-wells microplate assay using ciprofloxacin, a standard antibiotic. Chloroform, ethyl acetate, n-butanol and aqueous fractions showed excellent activity against Pseudomonas aeruginosa, (MIC at 7.1, 7.8, 5.6 and 5.3 respectively), which is greater than standard antibiotic ciprofloxacin (MIC 10.0). The antioxidant potential of all the fractions was evaluated. Ethyl acetate soluble fraction exhibited highest percent inhibition of DPPH radical as compared to other fractions. It showed 80.9 ± 1.2% inhibition of DPPH radical at a concentration of 30 ...

ANTIFUNGAL, CYTOTOXIC AND PHYTOTOXICITY OF AERIAL PART OF RANUNCULUS MURICATUS

OBJECTIVE: The present research is preliminary biological screening of aerial plant of Ranunculus muricatus (Ranunculaceae). Dichloromethane and methanol extracts of the aerial plant were investigated for their antifungal, phytotoxic and cytotoxic activities. METHOD: Anti-fungal, cytotoxicity and phytotoxicity activity was performed by Agar tube dilution assay, Brine shrimp lethality bioassay and lemna bioassay respectively. RESULT: Dichloromethane and methanolic extract exhibited significant phytotoxicity against Lemna minor having Paraquat as standard drug and incubation condition (28 ± 1ºC). None of extracts presented any significant cytotoxic activity having Imipenum and Etoposide as standard drug respectively. Both extract had non-significant antifungal activity but it has been noted that methanol extract showed 30% inhibition with linear growth at 70mm, when compared with control; only against Microsporum canis. CONCLUSION: Ranunculus muricatus showed significant phytotoxicity. The phytotoxicty assay is a valuable major screen for weedicide investigation. Additionally, modern studies are currently carried out to identify the allelopathic constituents by isolation, purification and structure elucidation to find out as effective herbicidal.

In Vitro Biological Activities Of Ranunculus gracilis Clarke Rhizome

Journal of anatolian environmental and animal sciences, 2022

Ranunculus gracilis rhizomes were picked from Yenice Forests Karabük province in Turkey. Ethanol was chosen for extraction solvent. Disc diffusion method including filamentous and non-filamentous Gram-positive bacteria, Gram-negative bacteria, and yeast strains was used to evaluate the antimicrobial activity of the extract. 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity and ferric reducing antioxidant power analysis were performed to determine antioxidant activity. The Folin-Ciocalteu method for determining the total phenolic amount and the AlCl3 method for the total flavonoid content of the extract was chosen. Mean diameters of inhibition zones (IZD) of the bacteria were found in the range of 8.2 mm to 24.45 mm. This value was measured as 17.82 mm and 18.69 mm for yeasts. The total antioxidant activity value of the extract was calculated as 7.08 mg AAE/g extract. The IC50 value was found as 9.097 mg/mL for DPPH free radical scavenging activity. The FRAP value indicated that the reducing power of 1 gram of sample was equivalent to 4.66 μmol of Trolox. The total phenolic content of ethanol extract of R. gracilis rhizomes was determined as 0.414 mg GAE/g, while the flavonoid content was calculated as 0.68 mgQE/g. This study is the first report demonstrating the biological activities of R. gracilis in the literature. The analyzed ethanolic extract of R. gracilis rhizomes demonstrated that the biological activity level could be considered significant according to the obtained results.

PHYTOCHEMICAL STUDY OF AERIAL PARTS OF RANUNCULUS MURICATUS FOR THE PHARMACOLOGICAL ACTIVE COMPOUNDS

Phytochemical study for the pharmacological activity is an important segment of drug designing. This initial work establishes a foundation for product development. The pharmacological active compounds are finally developed into a pharmaceutical preparation. This is used in actual clinical and therapeutical practice to cure miscellaneous pathological problems. Thus; we have aimed this study for the extraction of compounds using two solvents of different polarities, so that maximum number of compounds can be extracted. Analytical technique used for the isolation and identification of the various compounds is thin layer chromatography. Phytochemical analysis of aerial parts showed the presence of Tannins, saponin and cardiac glycosides. Cardioactive glycosides, Tannin and saponin were reported in aerial parts of Ranunculus muricatus for the first time. Therefore Ranunculus muricatus can be used for further isolation and structural determination of cardioactive compounds.