1H NMR Metabolomics of Chinese Human Milk at Different Stages of Lactation among Secretors and Non-Secretors (original) (raw)
Exploring human breast milk composition by NMR-based metabolomics
Natural Product Research, 2014
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Metabolites, 2013
Milk is a key component in infant nutrition worldwide and, in the Western parts of the world, also in adult nutrition. Milk of bovine origin is both consumed fresh and processed into a variety of dairy products including cheese, fermented milk products, and infant formula. The nutritional quality and processing capabilities of bovine milk is closely associated to milk composition. Metabolomics is ideal in the study of the low-molecular-weight compounds in milk, and this review focuses on the recent nuclear magnetic resonance (NMR)-based metabolomics trends in milk research, including applications linking the milk metabolite profiling with nutritional aspects, and applications which aim to link the milk metabolite profile to various technological qualities of milk. The metabolite profiling studies encompass the identification of novel metabolites, which potentially can be used as biomarkers or as bioactive compounds. Furthermore, metabolomics applications elucidating how the differential regulated genes affects milk composition are also reported. This review will highlight the recent advances in NMR-based metabolomics on milk, as well as give a brief summary of when NMR spectroscopy can be useful for gaining a better understanding of how milk composition is linked to nutritional or quality traits.
Journal of Maternal-Fetal and Neonatal Medicine, 2012
The aim of the present study was to investigate the metabolic profile of preterm human breast milk (HBM) by using a metabolomic approach. Methods: NMR spectroscopy and GC/ MS were used to analyze the water-soluble and lipid fractions extracted from milk samples obtained from mothers giving birth at 26-36 weeks of gestation. For the sake of comparison, preterm formula milk was also studied. Results: The multivariate statistical analysis of the data evidenced biochemical variability both between preterm HBM and commercial milk and within the group of HBM samples. Conclusions: The preliminary results of this study suggest that metabolomics may provide a promising tool to study aspects related to the nutrition and health of preterm infant.
2019
Proceedings of the 15 th International Workshop on Neonatology and the 40 th Congress UMEMPS (Union of Middle-Eastern and Mediterranean Pediatric Societies) • Cagliari (Italy) • October 24 th -26 th , 2019 • Children of the Middle-Eastern and Mediterranean area: we can do better! Guest Editors: Vassilios Fanos (Cagliari, Italy), Enver Hasanoglu (Ankara, Turkey), Michele Mussap (Cagliari, Italy), Robert Sacy (Beirut, Lebanon), Elie Saliba (Tours, France), Salvatore Vendemmia (Aversa, Italy) Nutrition in early life has important biological effects on immediate and lifetime health. In the light of these considerations, products such as specialized and standard infant formulas substitute for human milk have the potential to influence health outcomes differently depending on their composition. The recent knowledge of the long-term health benefits of breast-feeding has addressed research toward the creation of formulas ever closer to the needs of the infant both in term of nutritional and...
Nutrients, 2018
The composition of human breast milk is highly variable, and it can be influenced by genetics, diet, lifestyle, and other environmental factors. This study aimed to investigate the impact of geographical location and mode of delivery on the nuclear magnetic resonance spectroscopy (NMR) metabolic profile of breast milk and its relationship with the milk microbiome. Human milk metabolic and microbiota profiles were determined using NMR and 16S rRNA gene sequencing, respectively, in 79 healthy women from Finland, Spain, South Africa, and China. Up to 68 metabolites, including amino acids, oligosaccharides, and fatty acid-associated metabolites, were identified in the milk NMR spectra. The metabolite profiles showed significant differences between geographical locations, with significant differences ( < 0.05) in the levels of galactose, lacto--fucopentaose III, lacto--fucopentaose I and 2-fucosyllactose, 3-fucosyllactose, lacto--difucohexaose II, lacto--fucopentaose III, 2-hydroxybut...
Clinical Impact of Human Breast Milk Metabolomics
Clinica Chimica Acta, 2015
Metabolomics is a research field concerned with the analysis of metabolome, the complete set of metabolites in a given cell, tissue, or biological sample. Being able to provide a molecular snapshot of biological systems, metabolomics has emerged as a functional methodology in a wide range of research areas such as toxicology, pharmacology, food technology, nutrition, microbial biotechnology, systems biology, and plant biotechnology. In this review, we emphasize the applications of metabolomics in investigating the human breast milk (HBM) metabolome. HBM is the recommended source of nutrition for infants since it contains the optimal balance of nutrients for developing babies, and it provides a range of benefits for growth, immunity, and development. The molecular mechanisms beyond the inter-and intra-variability of HBM that make its composition unique are yet to be well-characterized. Although still in its infancy, the study of HBM metabolome has already proven itself to be of great value in providing insights into this biochemical variability in relation to mother phenotype, diet, disease, and lifestyle. The results of these investigations lay the foundation for further developments useful to identify normal and aberrant biochemical changes as well as to develop strategies to promote healthy infant feeding practices.
Tropical Animal Science Journal, 2021
The objectives of this study were to characterize and compare non-volatile polar metabolite profiles of bovine colostrum, collected within 1 h and at 72 h after parturition, from crossbred Holstein cows raised in northeastern Thailand. The colostrum serum was characterized and compared using a non-targeted proton nuclear magnetic resonance (1H-NMR) technique combined with chemometric analysis. Results demonstrated that the main effect of post-parturition time provided a significant impact on the physical properties and major chemical constituents of colostrum, while the influence of farm origin and sampling month were likely undetectable. The 1H-NMR technique enabled to identify 45 non-volatile polar metabolites in the samples. Partial least-squares-discriminant analysis (PLS-DA) allowed discrimination of colostrum metabolome not only according to different times after parturition, but also the origins of the farm as well as sampling months. Differential metabolites were statistical...
NMR metabolite profiles of dairy: A review
International Dairy Journal, 2018
Nuclear magnetic resonance (NMR) spectroscopy, which is one of the most powerful "omics" analytical platforms, has been broadly adopted recently in foodomics. 1 H NMR has been applied to the study of the metabolite profile of dairy products throughout the supply chain, in relation to different aspects such as animal health, milk quality, geographical origin and cheese ripening process. This review reports and discusses the literature on the topic, also collecting the identified metabolites in a descriptive table and depicting them in a Venn diagram for both milk and cheese; moreover, experimental details of the reviewed papers have been reported. The present review provides an exhaustive state-of-the-art in the field of dairy products, addressing both NMR experts and non-experts to the still unexplored potential applications of NMR in dairy characterisation, and in general in foodomics.
An untargeted metabolomics analysis of exogenous chemicals in human milk and transfer to the infant
2022
Human milk is the optimal infant nutrition. However, while human-derived metabolites such as lipids and oligosaccharides in human milk are regularly reported, the presence of exogenous chemicals (such as drugs, food, and synthetic compounds) are often not addressed. To understand the types of exogenous compounds that might be present, human milk (n=996) was analyzed by untargeted metabolomics. This analysis revealed that lifestyle molecules such as medications and their metabolites, food, industrial sources such as plasticizers, cosmetics, microbial molecules, and other personal care products are found in human milk. We provide further evidence that some of these lifestyle molecules are also detectable in the newborn's stool. Thus, this study gives important insight into the types of exposures infants receiving human milk might ingest due to the lifestyle choices, exposure, or medical status of the lactating parent.
Scandinavian Journal of Clinical & Laboratory Investigation, 2014
Background . Modern metabolomic profi ling has not yet been applied to human breastfeeding research. A common reason for breastfeeding cessation is perceived insuffi cient milk production. We investigated broad biochemical profi les in maternal urine collected during and after pregnancy to identify biomarkers related to reduced reported breastfeeding. Methods . Fasting urine was collected at three consultations (visit V1: gestational week 8 -20; V2: week 28 Ϯ 2; V3: 10 -16 weeks postpartum) in the STORK Groruddalen program, a prospective, multiethnic cohort study of gestational diabetes involving healthy, pregnant women in Oslo, Norway, and analyzed using NMR spectroscopy. Breastfeeding at V3 was recorded in three categories: Exclusively breastfeeding ( n ϭ 326), partially breastfeeding ( n ϭ 156) and formula feeding ( n ϭ 67). Results . Five metabolites were relevant to breastfeeding. Lactose was detected at V1 and increased to 0.1 mM/mM creatinine at V2. Postpartum excretion at V3 was signifi cantly higher in exclusively breastfeeding women than partially or non-breastfeeding (median ϭ 0.29, 0.23 and 0.04 mM/mM creatine, respectively; ANOVA p -value ϭ 2e -70). Glycine excretion at V3 (0.12, 0.10 and 0.06, respectively; p ϭ 2e -5) and at V2 were associated with breastfeeding (0.34, 0.33 and 0.26, respectively; p ϭ 4e -5). Creatine and two unidentifi ed substances also correlated with breastfeeding. NMR metabolomics found no other metabolites differing between categories during pregnancy (V1, V2), and did not predict individual breastfeeding postpartum (V3). Conclusion . Decreased glycine excretion at V2 may indicate diffi culties meeting the metabolic demands of the growing fetus, but urine profi les contained otherwise little indication of early adaptations during pregnancy towards reduced biological potential to breastfeed.