Jai Sunder et al /J. Pharm. Sci. & Res. Vol.3(8), 2011,1404-1407 Antibacterial Activity in Solvent Extract of Different Parts of (original) (raw)
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Antibacterial activity of water extracts different parts of Morinda citrifolia grown in Sri Lanka
International journal of scientific and research publications, 2016
This study examined the antibacterial potential of water extracts of different parts of Morinda citrifolia (Family: Rubiacae) plants which is used/indicated in Sri Lankan traditional and folk medicine to wash old cutaneous wounds, cuts, abrasion, rashes, bruises or burns, and as a throat gargle. This was tested in triplicate, in vitro, using agar disc diffusion bio assay against Gram positive human pathogenic bacteria, Staphylococcus aureus (ATCC 25923) and Gram negative bacteria , Escherichia coli (ATCC 35218). Concentration of water extracts tested were 500,750 and 1000 μg/disk. Gentamicin (10 μg/disk) was used as the positive control. The results showed that none of the extracts (roots, fruits, flower, stem bark or combination of these parts) was effective against E. coli whilst fruit (diameter of inhibition zone 2.93 ±0.03mm), stem bark (5.1 ±0.03mm) and combined extract (9.46 ± 0.03mm) exhibited antibacterial activity against S. aureus. The reference drug, Gentamicine induced a...
Antibacterial, Antioxidant and GC- MS Analysis of Morinda citrifolia Extracts
2016
The purpose of the study was to determine the antibacterial, antioxidant activities, and GC-MS analysis of aqueous and methanolic extracts from the fruit and leaves of Morinda citrifolia. Well diffusion, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays were used to test antibacterial activity against methicillin-resistant Staphylococcus aureus. DPPH (2, 2-diphenyl-1 picrylhydrazyl) and superoxide dismutase (SOD) assays were used to evaluate antioxidant activity. The hot and cold aqueous extracts of the fruit of M. citrifolia had no effect on all test bacteria but methanolic extracts of M. citrifolia had antimicrobial and antioxidant properties. GS-MS analysis revealed many important compounds such as anthraquinones, diterpenes, alkaloids and others in all extracts of this plant. It could be concluded that this plant had good antibacterial and antioxidant effects. The results suggest that this plant can be a new source of antimicrobials and a...
Antibacterial activity of water extracts of different parts of Morinda citrifolia grown in Sri Lanka
This study examined the antibacterial potential of water extracts of different parts of Morinda citrifolia (Family: Rubiacae) plants which is used/indicated in Sri Lankan traditional and folk medicine to wash old cutaneous wounds, cuts, abrasion, rashes, bruises or burns, and as a throat gargle.This was tested in triplicate, in vitro, using agar disc diffusion bio assay against Gram positive human pathogenic bacteria,Staphylococcus aureus (ATCC 25923) and Gram negative bacteria , Escherichia coli (ATCC 35218). Concentration ofwater extracts tested were 500,750 and 1000 µg/disk. Gentamicin(10 µg/disk) was used as the positive control. The results showed that none of the extracts (roots, fruits, flower, stem bark or combination of these parts) was effective against E. coli whilst fruit (diameter of inhibition zone 2.93 ±0.03mm), stem bark (5.1±0.03mm) and combined extract (9.46 ± 0.03mm) exhibited antibacterial activity against S. aureus. The reference drug,Gentamicine induced an inhibition zone of 13.98 ±0.04mm against S. aureus and 15.02 ±0.03 mm against E. coli. It is conducted that water extracts of different parts of M. citrifolia plant has mild to moderate antibacterial activity against S.aureus, a commonly encountered pathogen in cutaneous wounds.In addition, the results justify its use in Sri Lankan ethnomedicine as a topical cleaning agent for infected skin wounds.
Evaluation of antibacterial activity of Morinda citrifolia, Vitex trifolia and Chromolaena odorata
African Journal of Pharmacy and Pharmacology, 2012
Methanol, ethanol and ethyl acetate extracts of Morinda citrifolia L. (fruit, leaf and stem), Vitex trifolia and Chromolaena odorata (leaf) were prepared to evaluate the anti-bacterial activity. The antibacterial activities of extracts were tested against 12 bacterial isolates by in vitro disc diffusion method. Methanol extracts possessed higher level of antibacterial activity than ethanol and ethyl acetate extracts. When the plants were compared, C. odorata had better antibacterial activity than V. trifolia and M. citrifolia with the minimal inhibitory concentration (MIC) of about 25 mg/ml.
Phytochemical composition and in vitro biological activities of Morinda citrifolia fruit juice
Saudi Journal of Biological Sciences, 2020
Morinda citrifolia is a plant with broad nutraceutical and therapeutic effects and used in the traditional treatment of several ailments. The objective of this work is to investigate the phytochemistry of the fruit juice of M. citrifolia on one hand and on other hand to evaluate its antiradical and antibacterial activity. The phytochemical investigation was carried out by tube staining tests of the extract of two types of fruit juice of M. citrifolia. The antioxidant activity of these juices was evaluated by reducing the DPPH radical method. Concerning the antibacterial activity, it was tested on the in vitro growth of 10 reference bacterial strains using the well diffusion method. Qualitative phytochemistry of M. citrifolia fruit juices revealed the presence of large groups of secondary metabolites including polyphenols, reducing compounds, mucilage and terpernoids. The antioxidant activity of M. citrifolia fruit juices is dose-dependent and higher than that of ascorbic acid. Antim...
Chemical composition and antifungal activity of Morinda Citrifolia fruit extract
Bioscience Journal
Noni (Morinda citrifolia) fruit is a well-known plant used as a traditional medicine for preventing some diseases because of its abundance in chemical compounds. This research aimed to determine the phytochemical concentration, chemical composition, and antifungal activity of M. citrifolia fruit extract. M. citrifolia fruit was extracted with methanol and then distilled water for the partition extract. Subsequently, the extract was fractionated using various nonpolar to polar solutions, such as; chloroform, ethyl acetate, water, 2-propanol, and methanol fractions. Each fraction was evaporated until the dry extract was released. Additionally, the photochemical concentration of the M. citrifolia fruit extract was quantitatively determined using a UV-visible spectrophotometer. The chemical composition of the M. citrifolia fruit extract of each fraction was identified using gas chromatography-mass spectrometry (GC-MS). Then, the antifungal activity of M.citrifolia fruit extract against ...
Anti-infective potential of morinda citrifolia fruit extract
International Journal of Pharmacy and Pharmaceutical Sciences
Morinda citrifolia Linn (Rubiaceae), a plant with reported immunostimulant properties was selected for studying its anti-infective potential. The hydroalcoholic extracts of M. citrifolia fruits were evaluated in rats for their role in attenuation of physiological changes occurring due to the onset of E. coli induced sepsis. Results obtained from in-vitro studies indicated that the extract did not exert any anti-bacterial effect. However, the in-vivo studies revealed the ability of the extract to prevent alterations in four important physiological parameters namely-body temperature, pulse rate, breathing rate and WBC count. Our study indicates that the hydroalcoholic extract of Morinda citrifolia fruits is as effective as Gentamicin, a standard antibiotic used commonly for treatment of sepsis. This probably accounts for the widespread traditional usage of this plant as a febrifuge and anti-infective. Further studies need to be carried out to establish a correlation between its immunostimulant activity and anti-infective potential.
International Journal of Research Publications
Background: Noni fruit (Morinda citrifolia L.) has long been known by the people of Indonesia, especially Java, Sumatra, and Borneo. Noni fruit (Morinda citrifolia L.) is also widely used as traditional medicine in almost all islands in Indonesia. Noni fruit (Morinda citrifolia L.) is also known to have antibacterial activity against Staphylococcus aureus and MRSA (Methicillin-Resistant Staphylococcus aureus). The antibacterial activity of noni fruit is due to the phytochemical compounds found in noni fruit, such as iridoids, flavonoids, fatty acids, lignans, anthraquinone vitamins, and trace elements. Objective: This study is aimed to determine the antibacterial activity of the methanol extract of Noni fruit (Morinda citrifolia L.) against Staphylococcus aureus and MRSA (Methicillin-Resistant Staphylococcus aureus). Methods: This research was an experiment using a well-diffusion method to determine the antibacterial activity of the methanol extract of Noni fruit (Morinda citrifolia L.) against Staphylococcus aureus and MRSA (Methicillin-Resistant Staphylococcus aureus) in vitro. Minimum Bactericidal Concentration (MBC) values were determined using the diffusion method. The MBC values were determined by suspension streaking Muller Hinton broth on a mac Conkey agar plate. Five concentrations were used for the diffusion method, obtained from serial dilution using distilled water. The concentrations used were 100%, 50%, 25%, 12.5%, and 6.25%, respectively. The positive control of this study for Staphylococcus aureus was erythromycin 15 µg, and for MRSA was vancomycin 30 µg. Results: The Minimum Bactericidal Concentration (MBC) of methanol extract from Noni fruit (Morinda citrifolia L.) on Staphylococcus aureus and MRSA (Methicillin-Resistant Staphylococcus aureus) in this study was 6,25% and 3,13% respectively. Conclusion: This research shows a higher antibacterial activity against MRSA. The average diameter of the inhibition zone in each specific case was 0-26.64 mm for Staphylococcus aureus and 0-24.63 mm for MRSA. Therefore, further research is needed in developing Noni fruit (Morinda citrifolia L.) as an oral or topical antibiotic.
Acta Cirurgica Brasileira, 2014
To evaluate the antitumor and antimicrobial activity of ethanolic extract of Morinda citrifolia L. fruit cultivated in southeastern Brazil. METHODS: Preparation ethanolic extract of the fruit of Morinda citrifolia L. Culture of melanoma cells B16-F10 for treatment with ethanolic extract of Morinda citrifolia L. fruit to determine cell viability by MTT and determination temporal effect of ethanolic extract fruit on the cell growth B16-F10 for 8 days. Evaluation of antimicrobial activity of ethanolic extract fruit against Staphylococcus aureus and Escherichia coli by determination of Minimum Inhibitory Concentration (MIC). RESULTS: The ethanolic extract of Morinda citrifolia L. fruit (10mg/mL) decreased cellular activity and inhibited 45% the rate of cell proliferation of B16-F10 melanoma treated during period studied. The ethanolic extract of Morinda citrifolia L. fruit demonstrated antimicrobial activity inhibiting the growth of both microorganisms studied. Staphylococcus aureus was less resistant to ethanolic extract of Morinda citrifolia L. fruit than Escherichia coli, 1 mg/mL and 10 mg/mL, respectively. CONCLUSION: What these results indicate that the ethanolic extract of the fruit of Morinda citrifolia L. showed antitumor activity with inhibition of viability and growth of B16-F10 cells and also showed antibacterial activity as induced inhibition of growth of Staphylococcus aureus and Escherichia coli.
In vitro antimicrobial appraisal of the potentials of Morinda lucida against some selected bacteria
Food Research
The bioactive compounds from plant extract represent a potent alternative to combating the increasing antimicrobial resistance (AMR) to synthetic drugs. This study aimed to investigate the phytochemical, and antibacterial activities of Morinda lucida. Leaves of M. lucida was extracted successively using n-hexane, acetone, methanol and distilled water. The crude plant extracts were evaluated for their antibacterial activity against pathogens found in food and other sources such as Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Salmonella enterica serovar Typhi, Escherichia coli, and Klebsiella spp. using the agar well diffusion technique. Minimum inhibitory concentration (MIC) was carried out using the microbroth dilution technique. The phytochemical analysis showed the presence of alkaloids and sterols in all extracts. Flavonoid was only present in the n-Hexane extract. The result showed that n-Hexane extract had the widest zone of inhibition against P. aeruginosa...