Macroscopic and histopathological investigation of Sarcocystis infection in sheep (original) (raw)
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Animal and Veterinary Sciences, 2020
Background: Protozoal diseases caused by Sarcocystis species can cause serious damage in sheep and goats flocks, inducing decreased growth conversion rates and partial or complete condemnation of carcasses at the slaughter house. Sarcocystis spp. affecting sheep and goats are host specific for their intermediate hosts and family specific for their final hosts. They derive their name from the intramuscular cyst stage present in the intermediate host. Objective: Macro and micro identification of macro-cysts of Sarcocystis spp. affecting a Najdi sheep carcass. Method: Detection of macrosarcocystis during routine post mortem examination and identification the detected cyst by histopathological investigation. Result: The macroscopic sarcocystis were found cleared and scattered in almost all skeletal muscles including shoulder, chest and diaphragm as well as thigh. Both morphological and histopathological characteristics indicated that the detected cysts may be identified as Sarcocystis gigantea. Conclusion: It is a case study of macro-cysts of Sarcocystis spp. affecting local breed of Najdi sheep. It is considered a seldom detected case all over many years during meat inspection at Buraidah slaughter house. The possible control measures are those of simple hygiene. Farm dogs should not be housed in, or allowed access to, fodder stores nor should they be allowed to defecate in pens where livestock are housed. It is also important that they are not fed uncooked meat.
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine, 2011
According to the bibliographical studies' analysis, the incidence of ovine sarcocystosis, is situated in the first place reaching values of over 98%. The field researches concern the intensity and extent degree of the microsarcocysts in the skeletal muscles, in the following anatomic regions: the intercostal muscles, the diaphragm muscle, the tongue muscles, the dorsal muscles and the thigh muscles, with aspects regarding the incidence of these microcysts, depending on the animals' age. There were 160 meat samples collected from 20 ovine aged between 1,5 and 4 years. Following the samples' processing there was noticed that the extent of the Sarcocystis spp. infestation at the samples evaluated was of 100%, the average value of the intensity being the highest in the myocardia. In this context, it is necessary, in the case of ovine meat products, which do not involve in their technological process a thermic treatment, to priory make a control also to investigate the microcysts evaluation. At the same time, the ovine meat and organs administrated in the carnivore's diet must be thermic sterilized.
Prevalence and intensity of Sarcocystis spp. infection in animals slaughtered for food in Lithuania
Veterinární medicína, 2019
The exact prevalence of Sarcocystis spp. infection in animals slaughtered for food is unknown in Lithuania. Therefore, the present study was initiated to evaluate Sarcocystis spp. infection in the carcasses of cattle (n = 206), sheep (n = 61), pigs (n = 73) and horses (n = 72) raised in Lithuania for food. The prevalence and intensity of Sarcocystis spp. infection were assessed under light microscopy by analysing 1 g of stained and squashed muscle samples. All the investigated muscle types (oesophagus, diaphragm, heart, neck, jaw, back, leg and tongue) were found to have been infected with microcysts rather than with macrocysts. A high prevalence of infection was established in cattle (44.9–98.1%) and sheep (100%), whereas the prevalence of this infection in pigs (30.1–50.0%) and horses (34.7–63.9%) was considered to be moderate. Significant differences in the infection prevalence were detected in the majority of muscle groups of cattle and in some muscle groups of pigs and horses. ...
Prevalence of Sarcocystis in slaughtered sheep carcasses in AL-bieda, Libya
Sarcocystis is an obligatory intracellular protozoan parasite, which can infect human and animals. Sheep are intermediate hosts for Sarcocysitis species. Despite the importance of worldwide sheep production, little is known about the prevalence of Sarcocystis in domestic sheep (Barbary breed) in Libya. The main aim of the present study is to determine the prevalence of Sarcocystis in sheep in Al-beida city, Libya, as well as to identify parasite based on macro and microcysts examination. Thirty five sheep (25 male and 10 female) slaughtered at local abattoirs in Al-beida city. Muscle samples were collected from heart, esophagus and diaphragm (35 each) of sheep carcasses and examined by digestion method igesting pepsin and Hcl for bradyzoites observation in the organs by light microscope. The results showed that, microcysts were more prevalent than macrocysts which were detected in only one esophagus sample in agedmore than one year age. The digestion technique revealed positive results in all samples taken from heart, diaphragm and esophagus (100%, 97% and 94%) respectively. There were significant differences between different ages, while the prevalence of Sarcocystis was not influenced by sex and seasonal variations. It could be concluded that prevalence of Sarcocystisis significantly high in Al-beida city.
International journal of health sciences
This study aimed to determine and identify Sarcocystis spp. infection in sheep and goats in Misan governorate, Iraq. samples from oesophagus, diaphragm, tongue, and heart muscles were examined histologically for microscopic and macroscopic cyst , 70 out of 90 sheep 77.78 % had microscopic thick walled sarcocysts with mean 53 ×24.72 μm diagnosed as Sarcocystis tenella while 25 out of them 27.78 % had microscopic thin-walled sarcocysts with mean 49.45×33 μm diagnosed Sarcocystis arieticanis , esophagus showed the highest infection rate above all organs examined for thick – walled Sarcocystis while diaphragm was the highest for thin –walled Sarcocystis , the heart was the lowest infected organ for both type as 27.78% and 3.33% respectively . In goats 50 sample examined revealed 20 infected with thick walled sarcocysts only with different in distribution according to organs being high in esophagus 40% and lowest in heart 16% with mean measurement 60.61 × 37.75 μm diagnosed as Sarco...
Prevalence and histopathology of Sarcocystosis in slaughtered carcasses in southeast Iran
Journal of Advanced Veterinary and Animal Research
Objective: Sarcocystis spp. are common parasites and in terms of economics and pathogenicity in domestic animals is important. The purpose of this work was to define the rate of contamination of slaughtered carcasses of cattle to Sarcocystis using digestive and histopathological methods in southeast Iran. Material and Methods: In this descriptive laboratory study for 1 year, 500 carcasses were examined and isolated bradyzoites of Sarcocystis with the digest method. Also, tissue samples from the esophagus and diaphragm were considered for pathologic studies and stained with hematoxylin and eosin of sections of histopathological. Results: The results showed that the highest contaminations were in imported male animals aged 2-3 years old in the spring. There was a significant difference (p < 0.05) in the prevalence rate with the sex and race of cattle but no significant difference (p > 0.05) in the prevalence rate with age and season. Conclusion: Infection with Sarcocystis is common in oxen in this region. The imported cattle are more infected. It seems that racing and the environmental condition affect the prevalence of Sarcocystosis.
The prevalence of Sarcocystis infection in meat-production by using digestion method in Ahvaz, Iran
Introduction and objective: Sarcocystis is an obligatory intercellular protozoan parasite which can induce infection and clinical signs in carnivorous and herbivorous host. Humans acquire infection by ingestion of cyst or eating raw and undercooked infected meat or meat-production. The main goal of this study is to detect prevalence of the parasites in meat-production such as hamburger, sausage and hot dogs and determine its important role in human health. Materials and methods: Seventy five samples of hamburger, sausage and hot dogs (each 25 samples) were collected from retail stores in Ahvaz southwest of Iran. The samples were examined by digestion method using pepsin and HCl for detecting Sarcocystis parasite by light microscope. Results: Sarcocystis species were seen in 56% for hamburger, 20% for hot dogs and 8% for sausage. Conclusion: The results of this study indicated that the rate of the Sarcocystis sp. infection in meat-production is considerable and would be important for human. Many Sarcocystis sp. can infect meat many species from canine and feline definitive host. A molecular test such as PCR is needed to identify the parasite species. Digestion method is a sensitive, rapid and simple test for diagnosing Sarcocystis infection in meat. To prevent human infection, consumption of raw or under-cooked meat and meat-produced hyper endemic area should be avoided. Significance and impact of the study: Regarding to meat-born disease transmitted to human, to detect protozoa parasites in meat and their products can be important to prevent transmission of parasites which have cyst stage and promote hygienic level in human community.
Evaluation of sanitary and veterinary inspection results of slaughtered sheep in Poland in 2017-2019
2021
The aim of this study was to follow and evaluate the sanitary and veterinary inspection results of slaughtered sheep in Poland in the period 2017-2019. More than 175,000 sheep were slaughtered in Poland during this period. Post-mortem veterinary inspection revealed symptoms and/or lesions in 19,799 animals, which constituted 11.30% of all examined sheep. Of these, 134 animals (0.08%) were considered unfit for consumption. The most numerous cases were with foci of pus, contamination and congestion (5.07%) and fasciolosis (4.72%). The highest number of foci of pus, contamination and congestion in sheep carcasses were found in the Małopolskie and Podlaskie voivodeships. Most of the cases deemed unfit for consumption were found in the Podkarpackie (30), Świętokrzyskie (29) and Podlaskie (23) voivodeships, accounting for over 61% of all disqualified sheep carcasses in 2017-2019 in Poland.
Occurrence and Distribution of Sarcocystis Parasite Isolated from Sheep in Yazd Province, Iran
Introduction: Sarcocystis species are one of the most important meat borne parasites. Sarcocystosis causes several symptoms in human as well as numerous diseases with a high economic impact on livestock. In the current paper at first the results of a pilot study for determination of disease agent in the lambs of Yazd province in central Iran is presented. Then the status of parasite in red meat products in the study region is discussed. Materials and Methods: Muscles of 70 slaughtered sheep from both sexes and different ages were investigated for presence of parasite cysts from September to October 2013. Carcass inspection with naked eye at industrial slaughterhouses of Yazd for macroscopic cysts, and pepsin-digestion method for microscopic cysts was performed on common infected sites of infection. Results: No macroscopic cyst was seen at inspection. However, bradyzoites of parasite were observed in 97.14% of animals’ digested muscles. No significant difference between infection and age groups or sex of animals was observed. Conclusion: As humans are considered to be the final and intermediate host of different species of Sarcocystis and since parasite cysts are present at microscopic sizes, transmission of infection from lamb meat should come into consideration.