Microbiological profile and their antibiotic sensitivity pattern in patients of chronic suppurative otitis media at eastern tertiary care center of Nepal (original) (raw)
Related papers
2020
Background: Chronic suppurative otitis media (CSOM) is defined as a persistent infection of the middle ear with a perforated tympanic membrane and draining exudate for more than 6 weeks. Information about the organism responsible for COSM and their antibiotic susceptibility pattern is an important for effective treatment. Aim: This study aims to develop protocol for empirical treatment by determining aerobic bacterial profile and antibiotics susceptibility in patients of chronic suppurative otitis media (CSOM) at a secondary care hospital in North India. Material and Methods: A cross-sectional study was conducted at ENT department of the secondary care hospital in North India on 85 patients, middle ear discharge sample was collected under strict aseptic conditions and antibiotic susceptibility done as per Clinical Laboratory Standards Institute guidelines. Result: 85 ear swabs were collected, and 89 bacterial isolates were identified, of which 62 (72.94%) sample with mono-microbial ...
https://www.ijhsr.org/IJHSR\_Vol.6\_Issue.3\_March2016/11.pdf, 2016
Introduction: Chronic suppurative otitis media (CSOM) is the most common infection of the ear characterized by recurrent, purulent discharge from the middle ear through a nonintact tympanic membrane. Its incidence has remained relatively higher in developing countries Aim/Objectives: This study was carried out in department of Microbiology, Dr.S.C.G.M.C, Nanded to determine bacteriological profile and its antibiotic susceptibility in patients with CSOM Materials & Methods: A total of 470 patientsaged 2-60 years admitted to ENT ward from July 2014 to April 2015 were studied. Ear swabs were taken and cultured aerobically on blood agar and Mac Conkeyagar. The organisms isolated were identified by standard microbiological methods and antibiotic sensitivity pattern was determined Results: Out of 470 patients, 245(52.12%) were female and 225(47.87%) were male. Peak prevalence of otitis media was documented in age group 2-10 years (39.41%). Amongst 470 ear swabs, 460(97.87%) were culture positive and 30(6.52%) were having 2 isolates. Pseudomonas aeruginosa (37.95%) was the commonest isolate followed by Staphylococcus aureus (21.83%), Coagulase Negative Staphylococcus (18.16%), Klebsiella pneumoniae (15.51%) and Escherichia coli (8.36%). Antibiotics sensitive to P. aeruginosa were Imipenem (100%), Gentamicin (65.2%) and Tobramycin (61.7%) and those resistant were Ceftazidime (71.6%) and Piperacillin (59%). Both K. pneumoniae and E. Coli species showed highest sensitivity to Amikacin (96.8%) and Ciprofloxacin (65.3%) and resistance to Amoxyclav (62.2%), Cefotaxime (61.8%) and Ceftriaxone (61.3%). Amongst Gram positive isolates, Vancomycin (100%), Clindamycin (64.3%) and Cefoxitin (53.9%) were highly sensitive and Penicillin (11.2%) was most resistant drug Conclusion: Routine use of topical antibiotics for any case of CSOM as empirical therapy must be reviewed and judicial use of antibiotics is recommended to minimise antibiotic resistance
IP Indian Journal of Anatomy and Surgery of Head, Neck and Brain
Objective: To isolate and identify various bacterial pathogens in positive ear swab cultures of CSOM cases and to determine the antibiotic sensitivity pattern of different bacterial species identified. Study design: Prospective study Setting: J. L. N. Hospital & Research Centre, Bhilai (C.G). Materials and Method: The study was done on the population of Bhilai and surrounding region. A sample size of 120 cases attending the ENT OPD with the diagnosis of Chronic Suppurative Otitis Media and active ear discharge were included in the study. Detailed history, general physical examination, local systemic examination was done along with aural swab for Gram's staining and aerobic bacterial culture and drug sensitivity. The data were then compared with the relevant & available literature. Results: Most of the patients were in the age group of 11-20 years (38.33%), followed by 21-30 years age group (31.67%). More males (60%) were affected by the disease than females (40%). Unilateral (59.16%) infection was more common than bilateral (40.83%). Incidence of monobacterial (90%) etiology was more common than polymicrobial (9.1%). Staphylococcus aureus 67 (51.53%) was the most commonly isolated bacteria followed by the Pseudomonas 26 (20%). Antibiogram showed the most effective drugs in the present study are clindamycin, cotrimoxazole, ciprofloxacin, amikacin, chloramphenicol and ceftazidime Conclusion: Staphylococcus aureus is the most common pathogen isolated, followed by Pseudomonas. Clindamycin was the most effective antibiotic followed by cotrimoxazole, ciprofloxacin, amikacin, chloramphenicol and ceftazidime. Antibiotic sensitivity of the organisms varies according to geographical area and local practice regarding the choice of antibiotics. A continuous and periodic evaluation of microbiological pattern and their antibiotic sensitivity pattern in local area is helpful in prescribing empirical antibiotics for successful treatment of otitis media and thus minimizing its complications and emergence of resistant strains.
Saudi Journal of Pathology and Microbiology
Chronic suppurative otitis media (CSOM) is an important cause of morbidity in developing countries and a harbinger of many complications, out of which hearing loss; especially in children is a cause for concern. The present study focuses on the causative agents of CSOM and their antibiotic susceptibilities. The patients were found to be more in the first two decades of life (29.2%) and Pseudomonas aeruginosa was found to be the most common agent (49.7%) followed by Staphylococcus aureus (23.8%). The isolates were found to be susceptible to a number of antibiotics, which can be used to prevent the lifelong complications of CSOM. Timely culture and sensitivity helps in the management of these cases.
Aerobic Bacteriology of Chronic Suppurative Otitis Media in Rajsamand District of Rajasthan
https://www.ijrrjournal.com/IJRR\_Vol.5\_Issue.8\_Aug2018/Abstract\_IJRR0031.html, 2018
Objective: The objective of our study was to examine the aerobic bacteriological profile and antibiotic sensitivity pattern to locally available antibiotics in chronic suppurative otitis media (CSOM) in Rajsamand district of Rajasthan state in India.. Aural swabs were taken on the first day of attendance of the patients to ENT OPD before any local medication was given to the patient, using sterile cotton wool swabs and sterile ear specula and sent for culture and sensitivity. Results: A total of 150 cases of CSOM were selected for the study out of which 109 cases were of unilateral CSOM and 41 cases were having bilateral disease. Thus, a total of 191 swabs were taken for analysis. Out of 191 swabs processed, microbial growth was seen in 176 samples while 15 samples showed no growth. 121 (68.75%) samples showed mono-microbial growth while 55 (31.25%) samples showed poly-microbial growth. The peak incidence of CSOM was found in the age group 31-45 years (34.66%) followed by age group 16-30 years (27.33%). Females (62%) were more commonly affected than males (38%) and the female: male ratio was 1.6:1. Pseudomonas aeruginosa (38.63%) was the most commonly isolated bacterial pathogen followed by staphylococcus aureus (35.22%) and klebsiella sp. (10.22%). Conclusion: A thorough and precise knowledge of the etiological agents causing CSOM and their antimicrobial susceptibility is of essential importance for an effective and efficient treatment and thus in further prevention of both complications and development of antibiotic resistance which is becoming more common now a days.
2016
Introduction: Chronic Suppurative Otitis Media (CSOM) takes a lot of time in the hospital outdoors and operation theatres. It is a chronic disease which is associated with irreversible sequelae and serious intracranial and extracranial complications. Henceforth early & effective treatment needs to be instituted to avoid such complications. Materials & Methods: This study was planned at a tertiary care medical college to assess the bacterial etiology and antibiotic susceptibility profile of CSOM cases and to draw comparison with similar studies throughout India over the last four years. Isolation and identification of pathogen was done using aerobic culture followed by standard biochemical tests and antimicrobial susceptibility testing for commonly used antimicrobials. Results: The commonest pathogens isolated were Pseudomonas aeruginosa, Staphylococus aureus, Klebsiella spp., Escherichia coli, Coagulase Negative Staphylococci (CONS) and other gram negative rods mostly showing susceptibility to high end antibiotics like beta lactam-beta lactamase inhibitor, Carbapenems, Fourth generation cephalosporins & Glycopeptides. Conclusion: There is wide variation in antibiotic sensitivity profile over varied geographical areas and populations throughout the country. For better management of this disease, clinical classification as well as antibiotic susceptibility testing of the organisms is essential for making the right choice of antibiotics. The high rate of multiple drug resistance as well as high level of resistance to individual antibiotics is a cause of concern.
Indian Journal of Otology, 2012
Chronic Suppurative Otitis Media (CSOM) is one of the common ear diseases which can cause many complications if not treated properly. This study was done to determine isolates causing CSOM and their antibiotic susceptibility pattern. The study was carried out from November 2014 to April 2015. Ear swabs were collected & cultured from one hundred and twenty patients with CSOM attending the ENT Out Patient Clinic in Al-Hillah Teaching hospital. The specimens were cultured The isolated organisms were identified by standard microbiological methods. The antibiotic susceptibility testing of the isolates was done using the Kirby-Bauer disc diffusion method.CSOM was less common among elderly and women. Pseudomonas aeruginosa was the most common isolated bacteria (37.5%) followed by Staphylococcus aureus (20.83%), and Streptococcus pneumoniae (16.67%).The most effective antibiotics in this study were ciprofloxacin and ceftazidime , whereas the highest resistance levels were observed for trimethoprim, carbenicillin, lincomycin and erythromycin.
IP Innovative Publication Pvt. Ltd., 2018
Introduction: In the Otolaryngology Out Patient Department (OPD), Chronic Suppurative Otitis Media (CSOM) is the most common encountered otological disease. Staphylococcus aureus and Pseudomonas species are the most commonly encountered etiological agents. Untreated CSOM leads to complications such as facial nerve paralysis, lateral sinus thrombosis, labyrinthitis, meningitis and brain abscess. Materials and Methods: Patients with CSOM having unilateral or bilateral ear discharge for more than 3 months, attending Otolaryngology OPD or admitted in the wards were included in the study. Two swabs were collected from each patient with sterile precautions and submitted to the Microbiology Department. The samples were subjected to Gram’s stain and culture according to standard operative procedure. Antibiotic susceptibility testing was performed by Kirby-Bauer’s disc diffusion method as per CLSI guidelines. Results: A total of 520 samples were collected, out of which 474 samples showed growth. Staphylococcus aureus was the predominant isolate followed by Pseudomonas species. Staphylococcus isolates were highly sensitive to vancomycin and linezolid. Conclusion: Several studies have showed the predominance of different etiological agents in different geographical regions with different antibiotic sensitivity pattern. So, the knowledge of local etiological agent and its susceptibility pattern helps clinicians in treating the patients and to prevent its complications and drug resistance. Keywords: CSOM, Staphylococcus aureus, Pseudomonas aeruginosa, Antibiotic susceptibility pattern, Aerobic bacteria.
Otitis media (OM) is a major health problem in both developed and developing countries. This study taken to determine the pattern of bacterial isolates in Patients with chronic suppurative otitis media (CSOM) in Latur District, Maharashtra (India). A prospective study carried out in dept. of Microbiology, Govt. Medical College, latur among consenting patients with CSOM attending the ENT department over a period of 10 months. Informed consent was obtained from the patients for this study and microbiological analysis done. A total of 168 patients aged 5-70 years with chronic suppurative otitis media were accounted and total 201 bacteria isolated from ear discharge of 168 paients. In the present study, gram-negative bacteria were the dominant isolates (59.7%) while gram positive bacteria were 81(40.3%) The most Common causal organisms isolated were Pseudomonas aeruginosa 57 (28.35) followed by Staphylococcus aureus 49 (24.37%), Coagulase negative Staphylococcus 26(12.93 %), Klebsiella spp. 23(11.44 %).this study indicates the antibiogram trends of the most predominant bacterial isolates in otitis media. Pseudomonas aeruginosa and S. aureus showed high resistant rate (73.6-89.4%) to, tetracycline, Erythromycin and amoxicillin.while P. aeruginosa demonstrated 96.5 % sensitivity to norfloxacin and low level of resistance to gentamicin and ciprofloxacin.