Gonadal maturation of Indonesian leaffish (Pristolepis grootii) using pregnan mare serum gonadotropin and luteinizing hormone-releasing hormone analog (original) (raw)
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Effect of PMSG+AD hormone variation on the gonadal maturation of Pedih fish (Tor douronensis)
IOP Conference Series: Earth and Environmental Science, 2019
Pedih (Tor douronensis) is a freshwater fish that live in mountainous areas and swift rivers in the interior of Aceh. The aimed of this research was to analyze the dosage of PMSG+AD hormone orally for the gonadal maturation of pedih (Tor dauronensis). This research was conducted at technical implementation unit of fish breeding center Lukup Badak, Pegasing district, Aceh Tengah on Febuary to March 2018. The method used was Completely Randomized Design (CRD) with 4 level of treatment and three replications: 0 ml/kg feed, 0.3 ml/kg feed, 0.5 ml/kg feed and 0.7 ml/kg feed. The result of ANOVA test showed that different dosage of PMSG+AD hormone orally showed significant effect on the weight of broodstock, relative weight, gonad maturations index, eggs diameter, and relative eggs diameter. The result of Duncan test showed that the best dosage was found 0.7 ml/kg feed, but this treatment was not significantly different with the dosage of 0.5 ml/kg feed.
International Journal of Biological and Chemical Sciences
Sex control in tilapia should provide an invaluable benefit to aquaculture. The effects of different dietary levels of papaya carica seed meal powder on growth and gonad histology in Oreochromis niloticus larvae were studied to prevent early maturity and uncontrolled spawning. Four (4) dietary level of seed meal powder, varying from 0 g.Kg-1 as a control; 1 g.Kg-1 ; 2 g.Kg-1 to 3 g.Kg-1 were each tested in triplicate. The diet were isonitrogenous and were fed to twelve (12) experimental groups of 110 larvae weighing 0.019 g at a feeding rate of 4% body weight, three times a day for 30 days with the experimental diet and then after fed with the basal diet for other 60 days of the experiment. At the end of the experiment, 24 fish from all replicates were randomly sampled, slaughtered and dissected to remove testicles and ovaries. Testicular and ovarian sampleswere fixed in 10% buffered formalin for 24 h before the histological process. After 90 days of experiment, fish weight increased from 0.019 g ± 0.09 to 32.47g ± 0.02; 30.40g ± 0.05; 34.68 g ± 0.07 and 33.83 g ± 0.09 respectively for C. papaya dosage of 0; 1; 2 and 3, respectively. In fish treated with 0 and 1 g.Kg-1 of PSM, the testes showed different stages of spermatogenesis with germ cells moving up to the spermatozoa. Similarly, the ovaries showed follicles at different stages of folliculogenesis up to vitellogenesis. The main lesional changes observed concerned the testicles of fish fed with 2 and 3 g.Kg-1 PSM with in particular the scarcity of germ cells and spermatozoa. For females, the only observations concern vitellogenesis which are important for treatment doses of 0 and 1 g.Kg-1 , while they decrease in the group treated with 3 g.Kg-1 .
Indian Journal of Fisheries, 2019
The present study was conducted to examine the effects of three different hormonal treatment types i.e., pituitary extract (Pt), ovaprim (Ov) and human chorionic gonadotropin (HCG) + Metoclopramide (Met) on reproductive performance of Caspian shemaya, Alburnus chalcoides (Guldenstadt, 1772). To this end, three experimental treatments and one control group were considered. The experimental groups were administrated with different doses of Pt, Ov, HCG+Met as follows: Pt-2 mg kg bw (body weight)-1 , Pt-3 mg kg bw-1 , Pt-4 mg kg bw-1 , Ov-10 μg kg bw-1 , Ov-20 μg kg bw-1 , Ov-30 μg kg bw-1 , HCG+Met-1000 IU kg bw-1 , HCG+Met-2000 IU kg bw-1 , HCG+Met-3000 IU kg bw-1 and also a control group without any hormonal treatment. The highest values of oocyte weight and egg diameter were observed in groups administrated with Ov 10 μg kg bw-1 and HCG + Met (2000 IU kg bw-1) respectively. The highest values of absolute fecundity and relative fecundity were recorded for fish administrated with Ov (20 μg kg bw-1). The latency period and hour-degree for final maturation were lower in fish administrated with Ov (10 μg kg bw-1) compared to other experimental groups (p<0.05). There were no significant differences between experimental groups in terms of other assayed parameters. The results of the present study demonstrated higher efficiency of ovaprim in improving the reproductive performance of Caspian shemaya.
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Induced breeding of endangered spiny eel (Mastacembelus armatus) using PG extract
Progressive agriculture, 2018
Mastacembelus armatus is an important freshwater spiny eel of Bangladesh. The experiment was initiated to establish an induced breeding technique of the species with two induced breeding trials. In trial I, four different doses viz. 20, 40, 60 and 80mg PGkg-1 body weight of the fish were used to standardize the PG dose to ovulate the female M. armatus, and in trial II, the best dose identified in trial I was injected to fish once (whole dose) or twice with divided dose of PG at the rate of 30% and 70% at 6h interval to observe the effect of the mode of hormonal injection. Among the four doses applied in trial I, best result was obtained from 40mg PGkg-1 body weight in respect of ovulation rate (100%) of females and fertilization (93.00±2.00%) and hatching rates (58.30±3.50%) of eggs. In trial II, the females treated once (with the whole dose) or twice with divided dose of PG equally responded and no significant difference (p>0.05) was observed between the modes of hormone injection in respect of ovulation rate of females and fertilization and hatching rates of eggs. As M. armatus is considered as endangered fish, this induced breeding technique will help to conserve the fish as well as to produce seed in commercial hatcheries.
Jurnal Ilmiah Perikanan dan Kelautan, 2024
One of the problems in giant prawn cultivation is female giant prawns laying eggs during rearing. They will incubate their eggs for three weeks so that the energy from the expected feed for growth is used for egg development and other reproductive activities. Giving MPA hormone to giant prawns either by injection or oral could inhibit gonadal maturation and increase growth rate. The maturation process of the prawn ovary consists of the activation of some complex cellular mechanisms involving genes that regulate the stages of oocyte development. This study aimed to evaluate the response of gonadal maturity and the expression of the MrvWD-Kazal gene in giant prawns fed with a diet containing MPA hormone. The design used in this study was a completely randomized design with four treatments and five replications. The treatments were feeding a diet added with MPA with a concentration of 0 mg.kg-1 feed as control (K), 50 mg.kg-1 feed (P1), 100 mg.kg-1 feed (P2), and 150 mg.kg-1 feed (P3). MPA hormone at a concentration of 50-150 mg/kg feed could inhibit the gonadal maturation of female prawns. In 100 mg/kg of feed concentration showed the lowest gene expression level, indicating an inhibition of gonadal maturation molecularly. The administration of MPA hormone through the feed is a recommended method of aquaculture because it is more applicable and effective than by injection, even though it has a non-uniform impact on each individual.
Aquaculture, 1987
Induced spawning of maturing milkfish (Chinos chanos For&al) with gonadotropin-releasing hormone (GnRH) analogues administered in various ways. Aquaculture, 60: 303-310. The response of mature female captive milkfish to mammalian and salmon gonadotropinreleasing hormone analogues (mGnRH-A and sGnRH-A) was investigated. Prior to spawning, six groups of three females received (1) lo-16 B mGnRH-A from an osmotic pump implanted intraperitoneally (IP); (2) 100 M mGnRH-A from a cholesterol/cellulose pellet implanted IP; (3) 10 &kg mGnRH-A as an intramuscular (IM) injection; (4) lo-16 pg sGnRH-A from an osmotic pump implanted IP; (5) 100 B sGnRH-A from a cholesterol/cellulose pellet implanted IP, and (6) a cholesterol/cellulose pellet without armlogue implanted IP. The most effective treatment was 100 B sGnRH-A/fish given in a cholesterol/cellulose pellet; all (3/3) of the fish spawned. However, mGnRH-A was more effective (2/3) compared with sGnRH-A (l/3) if osmotic pumps were used to administer GnRH-A. If the dose and method of administration were not considered, then the sahnon and mammalian GnRH analogues were equally effective (62-67%) for induction of ovulation and natural spawning in milkfiih. Gonads of control fish regressed. At the doses tested, injections or pellet implantations were mom effective compared with osmotic pumps. All pellet-implanted and injected females responded to treatment and 75% (6/8) spawned; half (3/6) of the pump-implanted females spawned. Spawning occurred from 18 to 36 h after treatment.
Indonesian Aquaculture Journal, 2021
Indonesian leaffish, Pristolepis grootii (Bleeker, 1852), is an undomesticated freshwater fish species native to the rivers, flooded swamps, and tributaries of Indonesia. The fish is mainly captured for consumption. In order to prevent its extinction and supply its growing demands, the artificial breeding of the fish should be developed. The purpose of this study was to determine the optimum dose of luteinizing hormone-releasing hormone analog (LHRHa) for stimulating the female P. grootii gonadal development at a dosage of 0, 1, 10, and 50 µg kg-1 of fish. Female fish (20.0 ± 0.6 g) were adapted for 30 days in the rearing environment and then separated into 12 aquariums with six fish per aquarium. Fish were then reared for another 21 days and fed with Tubifex sp. The LHRHa injection was conducted twice on day-7 and 14. Fish bodyweight, gonadosomatic index, gonad histology, blood estradiol-17â, and FSH-â and LH-â gene expression were evaluated at day 0, 7, 14, and 21. The results sho...
AFRICAN JOURNAL OF BIOTECHNOLOGY , 2014
Two intramuscular injection strategies were performed for females of Liza ramada. The first strategy was applied with two injections; priming dose (CPE, 20 mg per fish). Then, the resolving dose (200 µg/kg of LHRH-a) was given after 24 h later. The second strategy was applied with two injections; the priming dose (3500 IU HCG per fish). Then, the resolving dose (200 µg/kg LHRH-a) was given after 24 h later. The three successful spawning attempts occurred within 3 days when the female received an extra dose of LHRH-a 100 µg/kg (3rd injection) from the second breeding protocol. In both strategies, the males received a single dose of LHRH-a. Ovarian biopsy of hormonal treated and control females were taken at different times after each treatment in order to monitor oocyte development and to determine the time of ovulation, since voluntary spawning was not expected. Measurements of oocyte diameters were carried out at 24 h intervals (0, 24, 48, and 72 h). Diameters of oocytes were measured with a hemacytometer under a light microscope. Mature oocytes with a diameter of 600 ± 50 were more appropriate to injection and spawning. After the injection of the first strategy, the egg diameters ranged from 650 to 680 mm with clear oocyte center and final dose of the resolving dose of the egg diameters were 700 ± 50 mm. After 48 h from the second injection of the first strategy, only one fish spawned. The total number of the spawned eggs ranged from 1 to 1.2 million/fish with no signs of fertilization having a diameter that ranged from 700 to 750 mm. The spawned unfertilized eggs were rounded colourless and transparent. In the second strategy and after the final injection of the resolving dose, the egg diameters were 800±30 µm. After the resolving dose of the first strategy, there was no response of spawning. It showed more successful spawning rather than the first one which showed deformed unfertilized eggs. At the second breeding protocol, fish spawned during the 48 h after the third injection dose. After 48 h, the first three fish were successfully spawned with fertilization rates of 1, 1.8 and 1.6 million eggs/spawn and the percentage of fertilization were 52, 75 and 64%, respectively, but without hatching, and all the fertilized eggs reached the gastrula stage. Control non-injected females were subjected to the same rearing conditions but did not spawn. Two replicate samples of 1 ml of eggs were taken for both control and injected fish. Regarding fatty acids profile, the results reported that the mono-unsaturated fatty acid (MUFA), oleic acid, was highly recorded in the fertilized eggs of the treated females with the second strategy injection while in the first strategy this was not detected except in the unfertilized eggs. The females that was treated with the second strategy injection had more fatty acids particularly the saturated fatty acid, highly unsaturated fatty acid and polyunsaturated fatty acid, (eicosapentaenoic acid (20:5n-3,EPA), docosahexaenoic acid (22:6n-3,DHA) and arachidonic acid (20:4n-6,ARA). The most significant depletions were observed in polyunsaturated fatty acids. Our results suggested that CPE, HCG and LHRH-a promote ovulation and spawning process for both scheduled induction and the frequency of hormone injection influence the fatty acid composition of normal, injected gonad and fertilized eggs of L. ramada in relation to egg quality.
Effect of Passiflora incarnata (L) extract on gonadal maturation in young tilapia (Oreochromis sp.)
Latin American Journal of Aquatic Research, 2017
The effect of Passiflora incarnata's extract (PE) on gonadal maturation in young tilapia (Oreochromis sp.) was evaluated by administering feed supplemented with PE during the first 90 days immediately after yolk sac assimilation. One hundred and fifty fishes with 0.01 ± 0.003 g average body weight were randomly distributed in ten tanks with the following duplicated treatments: commercial feed (NAT), NAT with 60 mg kg-1 of 17-MT (MET), and NAT supplemented with 31.10 mg, 62.30 mg and 124.60 mg of PE g-1 (P1, P2, P3 respectively). There were no significant differences (P > 0.05) in the average body weight or in the male-female sex ratio between the experimental treatments (P1, P2, P3) and the control (NAT). Statistical differences in the gonadosomatic index (GSI) and the percentage distribution of gonadal maturation stages (PDGMS) were observed in females of P2 and P3. With respect to the females of NAT, the treatments P2 and P3 presented a lower GSI and a lower percentage of females in stage III (GSI: 1.11 ± 0.88, 1.04 ± 0.99 and 1.71 ± 0.72; PDGMS: 45, 30 and 80%, respectively). No significant differences in GSI or PDGMS were observed in the males. The results suggest that the observed differences in GSI and PDGMS in females are unrelated to antinutritional effects. Instead, these differences could be due to a possible antiestrogenic effect attributed to the possible anti-aromatase action of some of its compounds. Future research focusing on the use of PE for reproductive control in tilapia is suggested.