Induced breeding of endangered spiny eel (Mastacembelus armatus) using PG extract (original) (raw)
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Advance Engineering Science, 2023
Fish gonadotropin, Induce breeding, Indonesian fish species. Indonesian leaffish (Pristolepis grootii) is one of Indonesia's endemic fish species that has the potential to be cultivated. The unavailability of quality seeds is a main obstacle in P. grooti farming. This study aimed to induce gonadal maturation of P. grootii. A total of 95 females (100.81 ± 0.78 g), reared in 15 aquaria (n=9), fed with a commercial pellet, and injected twice on the 7th and 14th day.with PMSG 10 IU.kg-1 of BW (T1), PMSG 10 + LHRHa 50 g.kg-1 of BW (T2), PMSG 20 (T3), PMSG 20 + LHRHa 50 (T4), and control (T5). Blood samples were collected from the caudal vein at 0, 30, and 60 days post-injection. The results showed that P grootii injected with a combination PMSG 20 + LHRHa 50 had the highest estradiol 17β (905.46±83.09 ρg.ml-1), total cholesterol (383.78±40.57 mg.dL-1), GSI (1.68±0.12 %), fecundity (2,946±174.72 egg.fish-1), and oocyte diameter (922.64±11.54 µm). The combination of PMSG 20 IU.kg-1 of BW + LHRHa 50 g. kg-1 of BW was the best treatment for gonadal maturation of P. grootii.
International Journal of Fisheries and Aquatic Studies, 2016
The spawning performance of spiny eel, M. pancalus was investigated by using different hormones. The water quality parameters were within optimum ranges such as average temperature, DO and pH were 29.13 oC, 4.19 mg/L and 8.32 respectively. Hormones were administered into females in two doses; a lower priming dose followed a 6 h interval by a higher resolving dose. In first dose, the female were injected ovaprim, HCG and PG at 0.25 ml/kg, 200 IU/kg and 30 mg/kg of body weight respectively. The second dose was applied to both male and female and female received double amount of hormone of the first dose. The male were administered a single dose at 0.5 ml/kg, 400 IU/kg and 60 mg/kg of ovaprim, HCG and PG respectively. It is indicated that the use of ovaprim was more effective in spawning (100%), fertilization (75%) and hatching (55%) of M. pancalus compared to the other stimulators.
The present study compared the effectiveness of the Carp pituitary gland extract (PGE) and the synthetic hormone Ovaprim on spawning success of the stinging catfish, Heteropneustes fossilis during induced breeding. The PGE hormone was administered at 6 mg/kg of body weight for females and 2 mg/kg of body weight for males. In contrast, Ovaprim was administered at 0.3 ml/kg body weight and 0.1 ml/kg of body weight for females and males, respectively. The spawning success was higher in the Ovaprim-induced individuals with better performance recorded at all stages of spawning including latency period, ovulation, fertilization, hatching and incubation period compared to the PGE-induced individuals. In the Ovaprim induced individuals, the latency period occurred within 10 hours while in PGinduced individuals, the latency was after 15 hours. Similarly, ovulation rate was 90% for Ovaprim injected fish but lower 78.7% for PGE injected fish. Higher rate of fertilization was observed in the eggs of Ovaprim treated fishes 86.7% compared to 69.2% in PGE induced fish. On the other hand, hatching rate was 76.9% in eggs spawned from Ovaprim induced individuals compared to 72.7% in PGE induced fish and the incubation period was also shorter at 3.5 h for eggs from Ovaprim-induced fish while the PGE induced fish eggs required a 5-h incubation period. Finally, the results showed that Ovaprim treated fish yielded better results compared the PGE treated fish in terms of ovulation, fertilization and hatching rates of H. fossilis.
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Large requirements of fish larvae, led the specialists at the Carja 1 farm to experience injection with pituitary extract from other species of cyprinids, silver carp pituitary. The experiment took place in the period from 2-25 June 2008. To verify the effectiveness of injections of silver carp pituitary suspension were used two experimental groups of 30 exemplars (1:1), the first group of females received a total dose of 2.9 mg/kg, and the second batch a dose of 4.5 mg/kg. The total dose used for breeding male is 2 mg/kg pituitary. During the Asian cyprinid reproductive guided aimed to determine: the proportion of breeding maturation, fertilization rates and survival during embryonic development index and the index of survival to hatch from eggs to larvae of 3-5 days (most representative for the whole process of reproduction). Determining the number of larvae from each batch of fertilized eggs, the ultimate goal of action is guided by reproductive hormonal stimulation with silver carp pituitary. The number of larvae obtained from experimental plots 3-5 days silver carp injected with silver carp pituitary extract (63,96 and 64,67 thousand) comparable with those obtained in experimental groups grass carp (36,21 and 31,14 thousand) and bighead carp (39,36 and 41, 34 thousand) was approximately 58% higher.
The present study furnishes the comparison on the performance of different inducing agents in the induced breeding of the stinging catfish, Heteropneustes fossilis. During the study two experiments were conducted in two different hatcheries of Bangladesh. In the experiment 1, pituitary gland extract (PGE) was administered at 6 mg/kg body weight of females and 2 mg/kg body weight of males. In contrast, ovaprim was administered at 0.3 ml/kg and 0.1 ml/kg body weight of females and males, respectively. On the other hand, in case of experiment 2, PGE was administered at the same rate as experiment 1 while ovaprim was administered at 0.5 ml/kg and 0.1 ml/kg body weight of females and males, respectively and human chorionic gonadotropin (HCG) was injected at 1000 IU/kg body weight of both male and female fishes. Breeding success was found to be higher in ovaprim treated individuals in both the experiments in all aspects including latency period, ovulation rate, fertilization rate, hatching rate and incubation period compared to that of PGE and HCG induced individuals. In the ovaprim induced individuals, the latency period was within 10 hours while in PGE and HCG induced individuals, the latency period was 15 hours. In addition, the present investigation also revealed that, ovaprim is more efficient in terms of ovulation, fertilization and hatching rates when using at a rate of 0.5 ml/kg body weight of female fishes than using at a rate of 0.3 ml/kg body weight of female fishes. Results of the present study would help the hatchery managers in managing the induced breeding programs of H. fossilis and other catfishes.
Aquaculture, 1991
Spawning of the two Indian major carps3 rohu (Labeo rohita) and mrigal (Cirrhinus mrigala) could be induced by fish gonadotropin releasing hormone (GnRH) purified from murrel (Channa punctatus) hypothalamus. The 'Linpe' method i.e., GnRH plus a dopamine antagonist, pimozide, was used in combination with Cd 2+. Channa GnRH (cGnRH) was injected in two different doses, 10 or 20 pg per kg body weight in combination with pimozide (1 mg per kg) and Ca2+ (300 pg per kg) with an interval of 6 h. Induced spawning occurred in all six sets at the same time while control fish failed to spawn, The total number of eggs produc$ was approximately 30 000-35 000 in mrigal and 50 000-60 000 in rohu. Percent of fertilized eggs in rohu wr' c about 80% whereas it was about 900/b in mrigal. The above mentioned combination of cGnRH, pimozide and Ca2+, was used to test germinal vesicle breakdown (GVBD) in catfish (Clarias batrachus) and perch (Anabas testudineus). Oocyte diameter was significantly increased in catfish (from 07 i f 0.08 to 1.03 k 0.05 mm, P< 0.005) and in perch (from 0.46 & 0.03 to 0.74 + 0.09 mm, PC 0.0 1) at 36 h in response to above mentioned treatment. Oocyte diameter remained unaltered in control fish. In catfish, 82Oh GVBD occurred between 40-44 h while in perch 67% GVBD could be noticed between 50-56 h in the treated fish. GVBD did not take place in control catfish and perch till after 10 days of observation. Results indicate that use of cGnRH in the 'Linpe' method with Ca2+ is highly satisfactory for induced breeding of Indian major carps and final maturation of catfish and perch ovary.
The present study furnishes the comparison on the performance of different inducing agents in the induced breeding of the stinging catfish, Heteropneustes fossilis. During the study two experiments were conducted in two different hatcheries of Bangladesh. In the experiment 1, pituitary gland extract (PGE) was administered at 6 mg/kg body weight of females and 2 mg/kg body weight of males. In contrast, ovaprim was administered at 0.3 ml/kg and 0.1 ml/kg body weight of females and males, respectively. On the other hand, in case of experiment 2, PGE was administered at the same rate as experiment 1 while ovaprim was administered at 0.5 ml/kg and 0.1 ml/kg body weight of females and males, respectively and human chorionic gonadotropin (HCG) was injected at 1000 IU/kg body weight of both male and female fishes. Breeding success was found to be higher in ovaprim treated individuals in both the experiments in all aspects including latency period, ovulation rate, fertilization rate, hatching rate and incubation period compared to that of PGE and HCG induced individuals. In the ovaprim induced individuals, the latency period was within 10 hours while in PGE and HCG induced individuals, the latency period was 15 hours. In addition, the present investigation also revealed that, ovaprim is more efficient in terms of ovulation, fertilization and hatching rates when using at a rate of 0.5 ml/kg body weight of female fishes than using at a rate of 0.3 ml/kg body weight of female fishes. Results of the present study would help the hatchery managers in managing the induced breeding programs of H. fossilis and other catfishes.
2021
This research investigated the effectiveness of <em>P. obscura</em> pituitary gland extract in hypophysation of <em>C. gariepinus</em>. The experiment was laid out in a Completely Randomized Design (CRD), using the pituitary gland of <em>C. gariepinus</em> as the control hormone. Data, such as Temperature, Dissolved Oxygen Concentration, and weight of eggs stripped, egg count/gram, latency period, incubation period etc. Data collected were subjected to Independent sample T-test, to statistically evaluate the effectiveness of the test (<em>P. obscura </em>pituitary gland extract) hormone in the hypophysation of <em>C. gariepinus</em>. The latency period of <em>P. obscura </em>was found to extend for 2 hours beyond those injected with <em>C. gariepinus </em>pituitary. The Pituitary gland extract was able to induced maturation ovulation for fertilization. The induced eggs were fertilized and incubated f...
Effect of carp PG doses on induced breeding of Shing,Heteropneustes fossilis (Bloch)
2001
Five hormone doses viz. 25, 50, 75, 100, and 125 mg of carp PG/kg of body weight of the recipient fish were tested and they were designated as T1 T2, T3, T4, and T5 respectively. Significantly higher fertilization (98%) and hatching rates (38%) were obtained from T3 (75 mg of carp PG extract/kg body weight). While T4 (100 mg of carp PG extract/kg body weight) and T5 (125 mg of carp PG extract/kg body weight) gave the highest (90%) ovulation rate. In June and July the highest fertilization rate of 96 and 96.4% respectively and hatching rate 42.5 and 48.7% respectively were obtained. In over all consideration carp PG extract at a dose of 75 mg/kg body weight appears to be the suitable dose for induced breeding of H. fossilis and June and July are the suitable time for its induced breeding.
In the present study, intramuscular injection of ovaprim-C was studied on the number of eggs/kg, fertilization rate and hatching percentage during April to June 2008 at Fish Seed Hatchery, Satyana Road, Faisalabad, Pakistan on Grass carp (Ctenopharyngodon idella). Fishes were spawned successfully following a single dose of injection of ovaprim-C (luteinizing hormone releasing hormone analogue) with 0.6 ml/kg for female and 0.2 ml/kg for male. Ova and milt were stripped simultaneously and mixture was stirred for 15 to 30 s during which fertilization occurred. Hatching occurred within 18 to 22 h after fertilization. Experiment was conducted in circular spawning tank with 2 m diameter. Regression analysis was applied to assess the body weight dependence of absolute fecundity (total ripe eggs in the ovary) and relative fecundity (number of eggs/kg). It was observed that body weight has positive influence on absolute fecundity (r = 0.926). Equations were developed to describe these relationships. If it is impossible to determine the absolute and relative fecundity, then these parameters can be determined from the body weight. The equations of these parameters are highly significant (P < 0.001) and can be used to estimate the absolute and relative fecundity with a fair amount of accuracy.