Solea senegalensis Research Papers - Academia.edu (original) (raw)
Five strains (CAIM 1831T, CAIM 1832, CAIM 1833, CAIM 1834 and CAIM 1836) were isolated from cultured sole (Solea senegalensis) in two regions of Spain, two strains (CAIM 404 and CAIM 1294) from wild-caught spotted rose snapper (Lutjanus... more
Five strains (CAIM 1831T, CAIM 1832, CAIM 1833, CAIM 1834 and CAIM 1836) were isolated
from cultured sole (Solea senegalensis) in two regions of Spain, two strains (CAIM 404 and CAIM
1294) from wild-caught spotted rose snapper (Lutjanus guttatus) in Mexico, and one strain (CAIM
1835) from corals in Brazil. The 16S rRNA gene sequences of the novel isolates showed similarity
to Vibrio ponticus (98.2–98.3 %, GenBank accession no. AJ630103) and to a lesser degree to
Vibrio furnissii (97.2–97.3 %, X76336) and to Vibrio fluvialis (96.9–97.1 %, X74703). Multilocus
sequence analysis clustered these strains closely together and clearly separated them from
phylogenetically related species of the genus Vibrio. Genomic fingerprinting by rep-PCR
clustered the novel strains according to their geographical origin. Phenotypic analyses showed a
large variation among the new strains, but many tests enabled them to be differentiated from other
species of the genus Vibrio. The mean DTm values between the strains analysed here and closely
related type strains were above 6.79 6C. The values between the novel isolates were below
2.35 6C, well outside the limit suggested for the delineation of a bacterial species. The
phenotypic and genotypic data presented here clearly place these new strains as a coherent
group within the genus Vibrio, for which we propose the name Vibrio alfacsensis sp. nov. with
CAIM 1831T (5DSM 24595T5S277T) as the type strain.