Sequence variation of latent membrane protein-1 of Epstein-Barr virus strains associated with hairy leukoplakia - PubMed (original) (raw)
Sequence variation of latent membrane protein-1 of Epstein-Barr virus strains associated with hairy leukoplakia
J M Palefsky et al. J Infect Dis. 1996 Mar.
Abstract
The Epstein-Barr virus (EBV) latent membrane protein (LMP)-1 is expressed in hairy leukoplakia (HL), but data on LMP-1 sequence variation of HL isolates are limited. Variation in the LMP-1 repeat region and presence of a 30-nt deletion were studied using DNA scrapings from 28 HL lesions. cDNAs from 3 different HL isolates were sequenced, 2 from lymphocyte cell lines (LCLs) generated using HL biopsy material. The deletion was found in 16 (57%) of 28 HL scraping, and multiple repeat region variants were seen in 13 scrapings (46%). HL LMP sequence were similar to those described in nasopharyngeal cancer and lymphoma tissues, including two motifs of four amino acid changes relative to B95-8 upstream and downstream of the repeat region, respectively. Generation of LCLs using HL biopsy material confirmed the ability of HL EBV strains to infect and transform lymphocytes.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials