Acetate kinase (original) (raw)
L'acétate kinase est une phosphotransférase répandue chez les bactéries et les archées, qui catalyse la réaction :Acétate + ATP phosphate d'acétyle + ADP. Elle utilise le cation magnésium Mg2+ comme cofacteur. L'acétate kinase intervient dans la glycolyse en cas d'excès de glucose, ainsi que dans la méthanogenèse pour convertir l'acétate en acétyl-CoA avec la phosphate acétyltransférase.
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| dbo:abstract | In molecular biology, acetate kinase (EC 2.7.2.1), which is predominantly found in micro-organisms, facilitates the production of acetyl-CoA by phosphorylating acetate in the presence of ATP and a divalent cation. Short-chain fatty acids (SCFAs) play a major role in carbon cycle and can be utilized as a source of carbon and energy by bacteria. Salmonella typhimurium propionate kinase (StTdcD) catalyzes reversible transfer of the γ-phosphate of ATP to propionate during l-threonine degradation to propionate. Kinetic analysis revealed that StTdcD possesses broad ligand specificity and could be activated by various SCFAs (propionate>acetate≈butyrate), nucleotides (ATP≈GTP>CTP≈TTP; dATP>dGTP>dCTP) and metal ions (Mg2+≈Mn2+>Co2+). Inhibition of StTdcD by tricarboxylic acid (TCA) cycle intermediates such as citrate, succinate, α-ketoglutarate and malate suggests that the enzyme could be under plausible feedback regulation. Crystal structures of StTdcD bound to PO4 (phosphate), AMP, ATP, Ap4 (adenosine tetraphosphate), GMP, GDP, GTP, CMP and CTP revealed that binding of nucleotide mainly involves hydrophobic interactions with the base moiety and could account for the broad biochemical specificity observed between the enzyme and nucleotides. Modelling and site-directed mutagenesis studies suggest Ala88 to be an important residue involved in determining the rate of catalysis with SCFA substrates. Molecular dynamics simulations on monomeric and dimeric forms of StTdcD revealed plausible open and closed states, and also suggested role for dimerization in stabilizing segment 235-290 involved in interfacial interactions and ligand binding. Observation of an ethylene glycol molecule bound sufficiently close to the γ-phosphate in StTdcD complexes with triphosphate nucleotides supports direct in-line phosphoryl transfer. The enzyme is important in the process of glycolysis, enzyme levels being increased in the presence of excess glucose. The growth of a bacterial mutant lacking acetate kinase has been shown to be inhibited by glucose, suggesting that the enzyme is involved in excretion of excess carbohydrate. A related enzyme, butyrate kinase, facilitates the formation of butyryl-CoA by phosphorylating butyrate in the presence of ATP to form butyryl phosphate. (en) L'acétate kinase est une phosphotransférase répandue chez les bactéries et les archées, qui catalyse la réaction :Acétate + ATP phosphate d'acétyle + ADP. Elle utilise le cation magnésium Mg2+ comme cofacteur. L'acétate kinase intervient dans la glycolyse en cas d'excès de glucose, ainsi que dans la méthanogenèse pour convertir l'acétate en acétyl-CoA avec la phosphate acétyltransférase. (fr) 酢酸キナーゼ（acetate kinase, AK）は、酢酸とADPの間でリン酸基の転移を触媒する転移酵素である。 ATP + 酢酸 ADP + (ja) |
| dbo:symbol | Acetate_kinase |
| dbo:thumbnail | wiki-commons:Special:FilePath/1tuy.jpg?width=300 |
| dbo:wikiPageID | 32018991 (xsd:integer) |
| dbo:wikiPageLength | 3975 (xsd:nonNegativeInteger) |
| dbo:wikiPageRevisionID | 1079492761 (xsd:integer) |
| dbo:wikiPageWikiLink | dbr:Carbohydrate dbr:Carbon_cycle dbr:Mutant dbc:Protein_families dbr:Enzyme dbr:Glucose dbr:Glycolysis dbr:Bacteria dbr:Cation dbr:Acetate dbr:Acetyl-CoA dbr:Cell_growth dbr:Kinase dbc:EC_2.7.2 dbr:Butyrate_kinase dbr:Phosphate dbr:Divalent dbr:Excretion dbr:Phosphorylation |
| dbp:caption | Acetate kinase homodimer, Methanosarcina thermophila (en) |
| dbp:interpro | IPR000890 (en) |
| dbp:name | Acetate_kinase (en) |
| dbp:pfam | PF00871 (en) |
| dbp:pfamClan | CL0108 (en) |
| dbp:prosite | PDOC00826 (en) |
| dbp:scop | 1 (xsd:integer) |
| dbp:symbol | Acetate_kinase (en) |
| dbp:width | 270 (xsd:integer) |
| dbp:wikiPageUsesTemplate | dbt:2.7-enzyme-stub dbt:EC_number dbt:Enzymes dbt:Infobox_protein_family dbt:InterPro_content dbt:Kinases dbt:Portal_bar dbt:Reflist dbt:Serine/threonine-specific_protein_kinases dbt:Short_description |
| dcterms:subject | dbc:Protein_families dbc:EC_2.7.2 |
| rdf:type | owl:Thing dbo:Biomolecule wikidata:Q206229 wikidata:Q8054 yago:Abstraction100002137 yago:Family108078020 yago:Group100031264 yago:Organization108008335 yago:YagoLegalActor yago:YagoLegalActorGeo yago:YagoPermanentlyLocatedEntity dbo:Protein yago:SocialGroup107950920 yago:Unit108189659 yago:WikicatProteinFamilies |
| rdfs:comment | L'acétate kinase est une phosphotransférase répandue chez les bactéries et les archées, qui catalyse la réaction :Acétate + ATP phosphate d'acétyle + ADP. Elle utilise le cation magnésium Mg2+ comme cofacteur. L'acétate kinase intervient dans la glycolyse en cas d'excès de glucose, ainsi que dans la méthanogenèse pour convertir l'acétate en acétyl-CoA avec la phosphate acétyltransférase. (fr) 酢酸キナーゼ（acetate kinase, AK）は、酢酸とADPの間でリン酸基の転移を触媒する転移酵素である。 ATP + 酢酸 ADP + (ja) In molecular biology, acetate kinase (EC 2.7.2.1), which is predominantly found in micro-organisms, facilitates the production of acetyl-CoA by phosphorylating acetate in the presence of ATP and a divalent cation. Short-chain fatty acids (SCFAs) play a major role in carbon cycle and can be utilized as a source of carbon and energy by bacteria. Salmonella typhimurium propionate kinase (StTdcD) catalyzes reversible transfer of the γ-phosphate of ATP to propionate during l-threonine degradation to propionate. Kinetic analysis revealed that StTdcD possesses broad ligand specificity and could be activated by various SCFAs (propionate>acetate≈butyrate), nucleotides (ATP≈GTP>CTP≈TTP; dATP>dGTP>dCTP) and metal ions (Mg2+≈Mn2+>Co2+). Inhibition of StTdcD by tricarboxylic acid (TCA) cycle intermedia (en) |
| rdfs:label | Acetate kinase (en) Acétate kinase (fr) 酢酸キナーゼ (ja) |
| owl:sameAs | freebase:Acetate kinase yago-res:Acetate kinase wikidata:Acetate kinase dbpedia-fr:Acetate kinase dbpedia-ja:Acetate kinase dbpedia-sh:Acetate kinase dbpedia-sr:Acetate kinase https://global.dbpedia.org/id/4Kz4i |
| prov:wasDerivedFrom | wikipedia-en:Acetate_kinase?oldid=1079492761&ns=0 |
| foaf:depiction | wiki-commons:Special:FilePath/1tuy.jpg |
| foaf:isPrimaryTopicOf | wikipedia-en:Acetate_kinase |
| is dbo:wikiPageRedirects of | dbr:ACKA dbr:EC_2.7.2.1 dbr:ATP:acetate_phosphotransferase |
| is dbo:wikiPageWikiLink of | dbr:Methanosarcina dbr:ACKA dbr:Weizmannia dbr:Corynebacterium dbr:List_of_EC_numbers_(EC_2) dbr:List_of_MeSH_codes_(D08) dbr:Dimethyl_dicarbonate dbr:EC_2.7.2.1 dbr:ATP:acetate_phosphotransferase |
| is foaf:primaryTopic of | wikipedia-en:Acetate_kinase |
