| dbo:abstract |
Group II introns are a large class of self-catalytic ribozymes and mobile genetic elements found within the genes of all three domains of life. Ribozyme activity (e.g., self-splicing) can occur under high-salt conditions in vitro. However, assistance from proteins is required for in vivo splicing. In contrast to group I introns, intron excision occurs in the absence of GTP and involves the formation of a lariat, with an A-residue branchpoint strongly resembling that found in lariats formed during splicing of nuclear pre-mRNA. It is hypothesized that pre-mRNA splicing (see spliceosome) may have evolved from group II introns, due to the similar catalytic mechanism as well as the structural similarity of the Group II Domain V substructure to the U6/U2 extended snRNA. Finally, their ability to site-specifically insert into DNA sites has been exploited as a tool for biotechnology. For example, group II introns can be modified to make site-specific genome insertions and deliver cargo DNA such as reporter genes or lox sites (en) |
| dbo:symbol |
Domain_X GIIM |
| dbo:thumbnail |
wiki-commons:Special:FilePath/IntronGroupII.jpg?width=300 |
| dbo:wikiPageID |
7916185 (xsd:integer) |
| dbo:wikiPageLength |
14774 (xsd:nonNegativeInteger) |
| dbo:wikiPageRevisionID |
1112937161 (xsd:integer) |
| dbo:wikiPageWikiLink |
dbr:Protists dbr:Pyrimidine dbr:Nucleotide dbc:Ribozymes dbr:Ribozyme dbr:In_vitro dbr:In_vivo dbr:Intron dbr:MRNA dbr:Open_reading_frame dbr:RNA_splicing dbr:Fungi dbr:Gene dbr:Maturase_K dbr:Small_nuclear_RNA dbr:Bacteria dbr:Database_for_bacterial_group_II_introns dbr:Domain_(biology) dbr:Adenosine dbr:Protein dbc:RNA dbr:Group_I_catalytic_intron dbr:Guanosine_triphosphate dbc:RNA_splicing dbr:Biotechnology dbr:Plants dbr:Group_III_intron dbr:Organelle dbr:Exon dbr:Lariat_(disambiguation) dbr:LtrA dbr:Twintron dbr:Spliceosome dbr:TRNA dbr:RNA_Tertiary_Structure dbr:RRNA dbr:Nucleophilic_attack dbr:Splice_site dbr:Splicing_(genetics) dbr:File:Domain-V-secondary-structure.svg |
| dbp:caption |
full secondary structure of group II intron (en) |
| dbp:extra |
180.0 (dbd:second) 300.0 (dbd:second) |
| dbp:interpro |
IPR013597 (en) IPR024937 (en) |
| dbp:name |
Domain X (en) Group II catalytic intron, D1-D4 (en) Group II catalytic intron, D5 (en) Group II intron, maturase-specific (en) |
| dbp:pdb |
4 (xsd:integer) 6 (xsd:integer) |
| dbp:pfam |
PF01348 (en) PF08388 (en) |
| dbp:pfamClan |
CL0359 (en) |
| dbp:rfam |
RF00029 (en) |
| dbp:rfamClan |
CL00102 (en) |
| dbp:symbol |
Domain_X (en) GIIM (en) Intron_gpII (en) group-II-D1D4 (en) |
| dbp:wikiPageUsesTemplate |
dbt:Cite_journal dbt:Refbegin dbt:Refend dbt:Reflist dbt:Short_description dbt:Infobox_rfam dbt:Pfam_box dbt:Self-replicating_organic_structures |
| dct:subject |
dbc:Ribozymes dbc:RNA dbc:RNA_splicing |
| gold:hypernym |
dbr:Class |
| rdf:type |
owl:Thing dbo:Biomolecule wikidata:Q206229 wikidata:Q8054 dbo:Protein |
| rdfs:comment |
Group II introns are a large class of self-catalytic ribozymes and mobile genetic elements found within the genes of all three domains of life. Ribozyme activity (e.g., self-splicing) can occur under high-salt conditions in vitro. However, assistance from proteins is required for in vivo splicing. In contrast to group I introns, intron excision occurs in the absence of GTP and involves the formation of a lariat, with an A-residue branchpoint strongly resembling that found in lariats formed during splicing of nuclear pre-mRNA. It is hypothesized that pre-mRNA splicing (see spliceosome) may have evolved from group II introns, due to the similar catalytic mechanism as well as the structural similarity of the Group II Domain V substructure to the U6/U2 extended snRNA. Finally, their ability to (en) |
| rdfs:label |
Group II intron (en) |
| owl:sameAs |
freebase:Group II intron wikidata:Group II intron https://global.dbpedia.org/id/4ktZk |
| prov:wasDerivedFrom |
wikipedia-en:Group_II_intron?oldid=1112937161&ns=0 |
| foaf:depiction |
wiki-commons:Special:FilePath/Domain-V-secondary-structure.svg wiki-commons:Special:FilePath/IntronGroupII.jpg |
| foaf:isPrimaryTopicOf |
wikipedia-en:Group_II_intron |
| is dbo:wikiPageRedirects of |
dbr:Domain_X dbr:Lariat_intron dbr:Targetron dbr:Group_2_intron dbr:Group_II_catalytic_intron |
| is dbo:wikiPageWikiLink of |
dbr:Metalloprotein dbr:Retron dbr:Ribozyme dbr:Index_of_genetics_articles dbr:Intron dbr:Prp8 dbr:Gene_silencing dbr:Geobacillus_stearothermophilus dbr:Nucleic_acid_tertiary_structure dbr:RNA_hydrolysis dbr:RNA_splicing dbr:Constructive_neutral_evolution dbr:Orthornavirae dbr:Bernard_Dujon dbr:MISL_RNA_motif dbr:Maturase_K dbr:U2_spliceosomal_RNA dbr:Database_for_bacterial_group_II_introns dbr:Alan_Lambowitz dbr:History_of_RNA_biology dbr:History_of_molecular_biology dbr:Lenarviricota dbr:Realm_(virology) dbr:Group_I_catalytic_intron dbr:HEARO_RNA_motif dbr:Acido-Lenti-1_RNA_motif dbr:Kappa dbr:Circular_RNA dbr:Group_III_intron dbr:Domain_X dbr:OLE_RNA dbr:Mobile_genetic_elements dbr:LtrA dbr:Orientia_tsutsugamushi dbr:Twintron dbr:Riboviria dbr:Lariat_intron dbr:Targetron dbr:Group_2_intron dbr:Group_II_catalytic_intron |
| is foaf:primaryTopic of |
wikipedia-en:Group_II_intron |
