| dbo:abstract |
La régulation par la tétracycline est une méthode d'expression génique inductible dans laquelle la transcription est activée ou désactivée de manière réversible en présence de l'antibiotique tétracycline ou l'un de ses dérivés (doxycycline). (fr) Tetracycline-controlled transcriptional activation is a method of inducible gene expression where transcription is reversibly turned on or off in the presence of the antibiotic tetracycline or one of its derivatives (e.g. doxycycline). Tetracycline-controlled gene expression is based upon the mechanism of resistance to tetracycline antibiotic treatment found in gram-negative bacteria. In nature, the Ptet promoter expresses TetR, the repressor, and TetA, the protein that pumps tetracycline antibiotic out of the cell. The difference between Tet-On and Tet-Off is not whether the transactivator turns a gene on or off, as the name might suggest; rather, both proteins activate expression. The difference relates to their respective response to tetracycline or doxycycline (Dox, a more stable tetracycline analogue); Tet-Off activates expression in the absence of Dox, whereas Tet-On activates in the presence of Dox. (en) L'attivazione trascrizionale controllata da tetraciclina è un sistema di espressione genica inducibile nella quale la trascrizione è attivata o disattivata in modo reversibile in presenza dell'antibiotico tetraciclina, o di uno dei suoi derivati (ad esempio doxiciclina). In natura, il promotore Ptet esprime il fattore TetR, il repressore, e il fattore TetA, costituito da una proteina in grado di pompare all'esterno della cellula l'antibiotico tetraciclina. Il sistema viene distinto in Tet-On o Tet-Off a seconda che l'attivazione trascrizionale del gene di interesse sia rispettivamente indotta o repressa dalla presenza di tetraciclina. (it) 四環素轉錄調控系統（Tetracycline-Controlled Transcriptional Activation）是一種能夠定時調控（開啓或關閉）特定基因表達的轉錄調控系統。該系統由一個順式作用元件和一個活性受四環素調控的特殊轉錄調控蛋白（反式作用因子）構成。當四環素或多西環素（Doxy）等四環素衍生物存在時，處於該系統調控下的基因表達會開啓或關閉。四環素轉錄調控系統基於革蘭氏陰性菌四環素抗性基因表達調控系統。四環素轉錄調控系統中，轉錄調控蛋白即是通過原核生物的TetA或TetR蛋白與病毒的轉錄激活蛋白結構域融合產生，而與之配對的順式作用原件則是以細菌的四環素抗性基因操縱子爲基礎構建的。四環素轉錄調控系統分爲兩種類型：Tet-On系統和Tet-Off系統。在Tet-On系統中，有四環素類化合物存在時，對應的基因表達會打開。而在Tet-Off系統中，有四環素類化合物存在時，對應基因的表達會關閉。 (zh) |
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| rdfs:comment |
La régulation par la tétracycline est une méthode d'expression génique inductible dans laquelle la transcription est activée ou désactivée de manière réversible en présence de l'antibiotique tétracycline ou l'un de ses dérivés (doxycycline). (fr) L'attivazione trascrizionale controllata da tetraciclina è un sistema di espressione genica inducibile nella quale la trascrizione è attivata o disattivata in modo reversibile in presenza dell'antibiotico tetraciclina, o di uno dei suoi derivati (ad esempio doxiciclina). In natura, il promotore Ptet esprime il fattore TetR, il repressore, e il fattore TetA, costituito da una proteina in grado di pompare all'esterno della cellula l'antibiotico tetraciclina. Il sistema viene distinto in Tet-On o Tet-Off a seconda che l'attivazione trascrizionale del gene di interesse sia rispettivamente indotta o repressa dalla presenza di tetraciclina. (it) 四環素轉錄調控系統（Tetracycline-Controlled Transcriptional Activation）是一種能夠定時調控（開啓或關閉）特定基因表達的轉錄調控系統。該系統由一個順式作用元件和一個活性受四環素調控的特殊轉錄調控蛋白（反式作用因子）構成。當四環素或多西環素（Doxy）等四環素衍生物存在時，處於該系統調控下的基因表達會開啓或關閉。四環素轉錄調控系統基於革蘭氏陰性菌四環素抗性基因表達調控系統。四環素轉錄調控系統中，轉錄調控蛋白即是通過原核生物的TetA或TetR蛋白與病毒的轉錄激活蛋白結構域融合產生，而與之配對的順式作用原件則是以細菌的四環素抗性基因操縱子爲基礎構建的。四環素轉錄調控系統分爲兩種類型：Tet-On系統和Tet-Off系統。在Tet-On系統中，有四環素類化合物存在時，對應的基因表達會打開。而在Tet-Off系統中，有四環素類化合物存在時，對應基因的表達會關閉。 (zh) Tetracycline-controlled transcriptional activation is a method of inducible gene expression where transcription is reversibly turned on or off in the presence of the antibiotic tetracycline or one of its derivatives (e.g. doxycycline). Tetracycline-controlled gene expression is based upon the mechanism of resistance to tetracycline antibiotic treatment found in gram-negative bacteria. In nature, the Ptet promoter expresses TetR, the repressor, and TetA, the protein that pumps tetracycline antibiotic out of the cell. (en) |
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Régulation par la tétracycline (fr) Attivazione trascrizionale controllata da tetraciclina (it) Tetracycline-controlled transcriptional activation (en) 四環素轉錄調控系統 (zh) |
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