The Spinothalamic System Targets Motor and Sensory Areas in the Cerebral Cortex of Monkeys (original) (raw)

Abstract

Classically, the spinothalamic (ST) system has been viewed as the major pathway for transmitting nociceptive and thermoceptive information to the cerebral cortex. There is a long-standing controversy about the cortical targets of this system. We used anterograde transneuronal transport of the H129 strain of herpes simplex virus type 1 in the Cebus monkey to label the cortical areas that receive ST input. We found that the ST system reaches multiple cortical areas located in the contralateral hemisphere. The major targets are granular insular cortex, secondary somatosensory cortex and several cortical areas in the cingulate sulcus. It is noteworthy that comparable cortical regions in humans consistently display activation when subjects are acutely exposed to painful stimuli. We next combined anterograde transneuronal transport of virus with injections of a conventional tracer into the ventral premotor area (PMv). We used the PMv injection to identify the cingulate motor areas on the medial wall of the hemisphere. This combined approach demonstrated that each of the cingulate motor areas receives ST input. Our meta-analysis of imaging studies indicates that the human equivalents of the three cingulate motor areas also correspond to sites of pain-related activation. The cingulate motor areas in the monkey project directly to the primary motor cortex and to the spinal cord. Thus, the substrate exists for the ST system to have an important influence on the cortical control of movement.

Introduction

The spinothalamic (ST) system has long been known to be a major route for transmitting information about pain, temperature and perhaps the state of internal organs to the cerebral cortex (Craig, 2003). It is generally agreed that the ST system is comprised of several components which originate largely from neurons in laminae I, V, and VII of the spinal cord. The axons of these spinal neurons terminate in multiple thalamic nuclei. Past attempts to trace this pathway from the spinal cord through the thalamus and then, to the cerebral cortex have encountered a number of technical and conceptual difficulties. These include, but are not limited to, major disagreements about thalamic nomenclature and thalamic boundaries. As a consequence, the cortical targets of the thalamic neurons which receive ST input have been the subject of continuing controversy (Willis et al., 2002; Craig, 2003).

Here we used anterograde transneuronal transport of the H129 strain of herpes simplex virus type 1 (HSV1) to trace the disynaptic pathway that transmits information from the spinal cord via the thalamus to the cerebral cortex. The H129 strain has been successfully used to reveal a wide variety of multisynaptic circuits including those in the visual system, trigeminal afferent pathways, central viscerosensory pathways, and subcortical pathways involving the basal ganglia and cerebellum (Zemanick et al., 1991; Barnett et al., 1995; Sun et al., 1996; LaVail et al., 1997; Garner and LaVail, 1999; Archin and Atherton, 2002; Kelly and Strick, 2003; Rinaman and Schwartz, 2004). Thus, this strain of HSV1 is uniquely suited for tracing the multiple components of the ST system.

Transneuronal transport of the H129 strain from cervical segments of the spinal cord consistently labeled neurons in multiple cortical areas of the contralateral hemisphere. The major sites containing labeled neurons include granular insular cortex (Ig), secondary somatosensory cortex (S2) and several regions buried within the cingulate sulcus. It is noteworthy that comparable cortical regions in humans consistently display activation when subjects are acutely exposed to painful stimuli. In one animal we used retrograde transport of a conventional tracer from the ventral premotor area (PMv) to identify the motor areas in the cingulate sulcus. Transneuronal transport of H129 from the spinal cord in this animal demonstrated that each of the cingulate motor areas receives ST input. Next, we performed a meta-analysis of imaging studies in humans and compared the sites of pain-related activation on the medial wall of the hemisphere with the sites of movement-related activation in the same region. This analysis demonstrated that the human equivalents of the three cingulate motor areas correspond to sites of pain-related activation. Because the cingulate motor areas project to the primary motor cortex and to the spinal cord, these observations suggest that the neural substrate exists for the ST system to have an important influence on the cortical control of movement.

Materials and Methods

This report is based on observations from three Cebus monkeys (Cebus apella; 2.2–2.7 kg; 2 males, 1 female). In all three animals, we placed multiple injections of the H129 strain of HSV1 into lower cervical segments of the spinal cord. In one animal, we also injected a conventional tracer into the arm area of the PMv before injecting virus into the spinal cord.

As detailed in prior publications, all surgeries were performed under deep general anesthesia (1.5–2.5% isoflurane) using aseptic conditions (Kelly and Strick, 2003; Dum and Strick, 2005). Analgesic (buprenorphine) and antibiotic drugs (ceftriaxone) were given postoperatively in accordance with the Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC) and the National Institutes of Health Guide for the Care and Use of Laboratory Animals. The protocol was approved by the Institutional Animal Care and Use Committee and the Biosafety Committee. Biosafety practices conformed to the Biosafety Level 2 regulations outlined in Biosafety in Microbiological and Biomedical Laboratories (Department of Health and Human Services publication No. 93–8395). Details of the procedures for handling virus and virus-infected animals have been published previously (Kelly and Strick, 2000, 2003).

Spinal cord injections.

A detailed description of our procedures for injecting tracers into the spinal cord has been presented in He et al. (1993). Briefly, we performed a laminectomy to expose the cervical segments of the spinal cord. With the dura open, dorsal root entry zones were used to identify segmental levels. We used a Hamilton syringe (30-gauge needle) to place 13–15 injection tracks into the lower cervical segments (C5-T1). Small amounts of the H129 strain of HSV1 (0.2 μl; 3.2 × 1010 pfu/ml; supplied by Dr. Richard D. Dix, Georgia State University, Atlanta, GA) were injected at three depths along each track. The three depths targeted the major concentrations of ST neurons in laminae I, V and VII (Apkarian and Hodge, 1989a). After all injections were completed, the spinal cord was covered with artificial dura and the incision was closed in anatomical layers. We set the survival time for virus injections at 4 d. In Cebus monkeys, this survival time is appropriate for anterograde transneuronal transport of virus from the injection site in the spinal cord to the thalamus and then, a second stage of anterograde transneuronal transport from the thalamus to the cerebral cortex (Zemanick et al., 1991; Kelly and Strick, 2003).

PMv injection.

In one animal, we identified the arm representation of the PMv with intracortical stimulation and injected this region with a conventional tracer, diamidino yellow (DY). Because of differences in transport times, the PMv injections were made 11 d before the spinal injections of virus. Our methods for mapping the PMv have been described previously (Dum and Strick, 2005). Briefly, the lateral surface of the cortex was exposed under isoflurane anesthesia. Then, the anesthesia was switched to Telazol (5–7 mg/kg/h) and butorphanol (0.01 mg/kg) for the duration of the intracortical mapping. The exposed cortex was photographed. Trains of stimuli (33 cathodal pulses, 0.2 ms duration, 333 Hz frequency, 1–40 μA intensity) were delivered at a standard depth (1500 μm) with parylene-insulated Elgiloy microelectrodes. The movement evoked at each stimulation site was determined by visual inspection and muscle palpation. We defined the threshold current for each response as the stimulus intensity that evoked movement in ∼50% of the trials. The location of each penetration, the depth of stimulation, the movement evoked and its threshold were entered into a computer program that generated maps of these results.

Once the arm area of the PMv was identified, we used a Hamilton syringe to inject DY (2% in distilled water) into three sites. At each site, we placed small amounts of tracer (0.1–0.3 μl) at two depths (1100 μm, 1500 μm) below the cortical surface. When the injections were completed, the cortical surface was covered with artificial dura and the incision was closed.

Histological procedures.

At the end of the survival period, each animal was deeply anesthetized (ketamine, 20–25 mg/kg, i.m. followed by sodium pentobarbital, 40 mg/kg, i.p.) and was perfused transcardially with a 3 step procedure. The perfusates included: (1) 0.1 m phosphate buffer; (2) 10% buffered formalin; and (3) 10% buffered formalin with 10% glycerol added. After the perfusion the segmental levels of the spinal cord were marked with India ink at the junctions of dorsal root entry zones. The brain and spinal cord were blocked, photographed and stored at 4°C in buffered 10% formalin with 20% glycerol for 5–7 d.

Serial frozen sections (50 μm) of the spinal cord and cerebral cortex were cut in the transverse plane. Every tenth section was stained with cresyl violet to reveal cytoarchitecture. To identify virus-infected neurons, we processed at least every fourth section as free-floating tissue according to the avidin-biotin peroxidase method (Vectastain, Vector Laboratories). We detected HSV1 using a commercially available polyclonal antibody (diluted 1:2000; Dako) (Kelly and Strick, 2003).

Analytic procedures.

Brain and spinal cord sections were examined under the microscope for virus infected neurons using brightfield illumination and for DY labeled neurons using epifluorescence illumination (Olympus filter U-MWBV2). Section outlines, labeled neurons and injection sites were plotted using a computer-based charting system (MD2, Accustage) (Dum and Strick, 1991, 2005). We charted at least every fourth section for virus infected neurons or virus infected and DY labeled neurons. Software written in our laboratory allowed us to construct maps showing the location and density of labeled neurons in the cerebral cortex. The procedures we used to generate unfolded maps of cortical regions have been described in detail previously (Dum and Strick, 1991, 2005). Briefly, for charted sections we marked major morphological features (e.g., surface and depths of sulci) and drew a curved line along the border between cortical layers III and V. Then, our computer program used this information along with the location of labeled neurons to create flattened maps of cortical regions. To examine the density of labeled neurons, the computer program divided labeled regions into 200 μm bins and totaled the number of labeled neurons in each bin. This number was converted to a color code for display.

Images of selected anatomical structures were obtained using a digital camera (RT3 monochrome camera, Diagnostic Instruments) coupled to a personal computer. The images were adjusted for contrast, brightness and intensity using Corel Photopaint. In some instances, multiple images were formed into a composite with the “Stitch” function built into Corel Photopaint. Separate composites were produced from sections processed for virus and from adjacent sections processed for cytoarchitecture. The two composite images were aligned using blood vessels and major morphological features and then digitally merged into a single image.

Analysis of injection sites.

H129 injection sites display concentric central and peripheral zones of immunoreactive labeling (Zemanick et al., 1991; Kelly and Strick, 2003). The central zone, located adjacent to the needle track, is characterized by marked tissue necrosis and dense accumulations of reaction product but no discernible neurons. The peripheral zone contains darkly labeled neurons that can be distinguished from the background staining of the neuropil. We used the central zone of labeling to define the H129 injection sites in the spinal cord. To define the location of the central zone we examined sections of spinal cord at 1 mm intervals through the cervical enlargement.

DY injection sites also display central and peripheral zones of fluorescent labeling. We defined the DY injection site as the central zone containing the injected tracer (Zone 1) and the surrounding region of intense staining of neurons, glia and neuropil (Zone 2) (Huisman et al., 1983; Kuypers and Huisman, 1984; Condé, 1987; He et al., 1993). Based on this definition, we reconstructed the DY injection site and aligned it to the motor map of the PMv using the injection tracks as a guide.

Results

We injected the H129 strain of HSV1 into lower cervical segments of the spinal cord in cebus monkeys. We set the survival time following the virus injections at 4 d to allow for two stages of anterograde transneuronal transport (Zemanick et al., 1991; Kelly and Strick, 2003). In the first stage of transport, virus is taken up by first-order neurons at the injection site and transported in the anterograde direction to the thalamus where it moves trans-synaptically to infect second-order neurons (Fig. 1A). Then, in a second stage, virus is transported in the anterograde direction from second-order neurons in the thalamus to the cerebral cortex where it moves trans-synaptically to infect third-order neurons (Fig. 1).

Figure 1.

Experimental paradigm and cortical labeling. A, Diagram of anterograde transneuronal transport of virus from spinothalamic neurons in laminae I, V, and VII to thalamus and then from thalamus to cerebral cortex. The gray area in the spinal cord represents the virus injection site. B, C, Photomicrographs of third-order neurons labeled in the cingulate sulcus after anterograde transneuronal transport of virus from cervical segments of the spinal cord. The boxed area in B is enlarged in C. CgS, Cingulate sulcus.

Injection sites in the spinal cord

In two animals (DL2, DL3), the injection site included most of the dorsal horn and large portions of the intermediate zone throughout the cervical enlargement (C5-T2) (Fig. 2). In each case, the injection site was confined to the ipsilateral side and involved all the laminae (I, V, VII) in the lower cervical segments that are known to be the origin of spinothalamic neurons (Apkarian and Hodge, 1989a; Willis et al., 1979, 2001). In the third animal (DL7), the injection site was more confined (Fig. 2). It involved fewer segments (C7-T1) and did not completely fill the dorsal horn and intermediate zone. Consequently, DL7 was not included in the quantitative analysis of the distribution of cortical labeling. However, the major cortical areas that contained labeled neurons in DL7 were the same as those in the other two animals.

Figure 2.

Virus injection sites in the spinal cord. Upper left: We divided the gray matter of the spinal cord into eight regions and determined the extent of the spread of virus at the injection site into each region. Upper right and bottom panels, Each panel displays the injection site of a separate animal. Dark gray shading indicates the central zone of virus labeling and light gray indicates the peripheral zone. The injection was considered to be the central zone of labeling (see Materials and Methods for details). The segments sampled are indicated along the abscissa of each chart. We sampled each segment at 1 mm intervals.

Thalamic targets of the ST system

Our analysis of thalamic labeling has been limited to confirming that infected neurons were located at the known sites of termination of spinothalamic input. This is because a survival time sufficient to label third-order neurons in the cerebral cortex, will label not only second-order neurons in the thalamus, but also third-order neurons as well (Zemanick et al., 1991; Kelly and Strick, 2003). In short, we found labeled neurons at all the major sites of spinothalamic termination (Figs. 3, 4) (Ganchrow, 1978; Berkley, 1980; Mantyh, 1983; Apkarian and Hodge, 1989b; Gingold et al., 1991; Stepniewska et al., 2003; Craig, 2004). These included the caudal subdivision of the ventral posterior lateral nucleus (VPLc), the inferior portion of the ventral posterior nucleus (VPI), the posterior-suprageniculate complex (Po-SG), the central lateral nucleus (CL), and caudal portions of the medial dorsal nucleus (MD) (Figs. 3, 4). The region with labeled neurons that we designated as Po-SG is thought to include a subdivision termed the posterior portion of the ventral medial nucleus (VMpo) (Craig, 2004; see also Gingold et al., 1991). We also observed scattered small clusters of labeled neurons in the caudal portion of the oral subdivision of the ventral posterior lateral nucleus (VPLo), the caudal subdivision of the ventral lateral nucleus (VLc), the oral subdivision of the pulvinar (Pul.o) as well as in some midline nuclei (e.g., reuniens, central medial, submedius). Thus, the pattern of thalamic labeling is consistent with transneuronal transport of virus via all components of the spinothalamic system.

Figure 3.

Distribution of virus infected neurons in the thalamus. Photomicrographs show neurons in various nuclei of the thalamus that were infected by anterograde transneuronal transport of virus from the cervical spinal cord. A, B, Nissl stained sections of the thalamus show nuclear borders. Some gliosis is evident in regions where virus infected neurons are located (e.g., VPI in A; MD in B). C, D, Sections reacted to demonstrate virus infected neurons. Section C is near section A; section D is near section B. Black arrows mark distinct patches of labeled neurons. Open arrows in C and D mark blood vessels that are also present in A and B. E, F, Digitally merged photographs of the sections A + C and B + D. The calibration in F applies to all sections. CM, Centromedian nucleus; fr, fasciculus retroflexus; Hb, habenular nucleus; LP, lateral posterior nucleus; Pf, parafasciculus nucleus; VPM, ventral posterior medial nucleus.

Figure 4.

Virus infected neurons in thalamic nuclei. Photomicrographs of virus infected neurons in thalamic nuclei. VPI and VLc/VPLc are taken from the dashed boxes in Figure 3C. Po-SG and MD are taken from the dashed boxes in Figure 3D. The inset in Po-SG displays a higher-magnification view of an infected neuron (marked by the small arrow). The calibration for all sections is shown in MD. Abbreviations as in Figure 3.

Spinal input to other subcortical sites

Although the focus of this report is spinothalamic projections to the cerebral cortex, we also observed labeling at all the subcortical sites known to be the target of spinal input (not illustrated) (Yezierski, 1988; Craig, 1995; Newman et al., 1996; Andrew et al., 2003). For example, we found large numbers of labeled neurons in regions of the brainstem including the parabrachial nuclei, dorsomedial and ventrolateral medullary reticular formation, locus ceruleus, subceruleus, Kölliker-Fuse, solitary nucleus and periaqueductal gray (Yezierski, 1988; Craig, 1995; Andrew et al., 2003). In addition, we observed labeled neurons in basal ganglia and limbic areas known to be the target of spinal input including the globus pallidus, central nucleus of the amygdala, substantia innominata and the hypothalamus (Newman et al., 1996). The presence of infected neurons at these sites raises the possibility that the cortical labeling we observed could be mediated by routes other than a spino-thalamocortical pathway, such as a “postsynaptic dorsal column system” (Hayes and Rustioni, 1981; Al-Chaer et al.,1998, 1999; Al-Chaer et al.,1998, 1999; Willis et al., 1999), a spino-reticular formation-thalamocortical pathway (Yezierski et al., 1987; Craig, 1995; Gauriau and Bernard, 2002), or a spino-parabrachial nucleus-thalamocortical pathway (Pritchard et al., 2000, Gauriau and Bernard, 2002). We can rule out transport to the cortex via these pathways because they require transneuronal transfer through three synapses rather than the two required of the spino-thalamocortical pathway. In other words, the cortical neurons labeled via a spino-reticular formation-thalamocortical pathway would be fourth-order neurons rather than the third-order neurons labeled via the ST system. Only the hypothalamus also has the potential to mediate a disynaptic route from the spinal cord to the cerebral cortex (Kievit and Kuypers, 1975; Burstein et al., 1987; Newman et al., 1996; Rempel-Clower and Barbas, 1998; Barbas et al., 2003). However, we can rule out transport via this structure because the major cortical targets of the hypothalamus, i.e., regions of prefrontal and orbitofrontal cortex (Barbas et al., 2003) lacked labeled neurons in our experiments (see below).

Cortical targets of the ST system

Anterograde transneuronal transport of HSV1 from the spinal cord infected clusters of third-order neurons at multiple sites in the contralateral cerebral cortex (Figs. 5–7). The infected neurons were generally filled with reaction product and showed little or no evidence of cell lysis (Fig. 1B,C). The labeled cells varied in size, and were largely pyramidal-shaped. In regions of dense labeling, infected neurons were most concentrated in layer V with additional populations of labeled neurons in layers III–IV and VI.

Figure 5.

Distribution of cortical neurons labeled by anterograde transneuronal transport of virus from the spinal cord. Charts display dense clusters of labeled neurons (small black dots) found on coronal sections at various levels of the cerebral cortex. The numbers below each section indicate their location (marked in Figs. 6, 7). ArSi, ArSs, Inferior and superior limbs of the arcuate sulcus; ArSpur, spur of the arcuate sulcus; CgS, cingulate sulcus; LS, lateral sulcus; 1, 3a, 3b, 4, cytoarchitectonic areas.

Figure 6.

ST projections to cortical areas in the lateral sulcus. A, Lateral view of the left hemisphere from a cebus monkey. The lateral sulcus is unfolded to show the cortical areas buried in it. The lateral sulcus is unfolded downward from its dorsal edge. The dorsal and ventral edges of the sulcus are represented by solid lines and various bends in the sulcus are represented by dashed lines. Dotted lines enclose S2 on the dorsal bank of the lateral sulcus (LSd) and Ig on the Insula. B, C, Location and density of neurons labeled in the lateral sulcus following anterograde transneuronal transport of virus from the spinal cord (B, animal DL2; C, animal DL3). The density of labeled neurons was determined by counting the number of labeled cells within 200 μm bins along each section. The number of cells per bin was divided into five levels and color coded: white, top 5% of bins (95–100%); yellow, 90–95%; red, 80–90%; large blue squares, 40–80%; 0–40%, omitted. The numbered arrows refer to sections illustrated in Figure 5. AC, Level of the anterior commissure; ArS, arcuate sulcus; CSl, lateral extent of central sulcus; Ia, agranular insular cortex; Id, dysgranular insular cortex; IPS, intraparietal sulcus; LSv, lateral sulcus, ventral bank; PS, principal sulcus; 7op, opercular portion of area 7. Other abbreviations and conventions as in Figures 4 and 5.

Figure 7.

ST projections to cortical areas in the cingulate sulcus. A, Unfolded map of medial wall of the hemisphere. The medial wall is unfolded and reflected upward from the midline. The edges of the cingulate sulcus are represented by solid lines and the fundus is represented by a dashed line. A dotted line encloses the portion of the primary motor cortex that extends onto the medial wall. The numbers and letters in italics indicate the relative location of the various cortical areas that are on the medial wall. B, Location and density of neurons labeled in the cingulate sulcus following anterograde transneuronal transport of virus from the spinal cord (animal DL3). The numbered arrows refer to sections illustrated in Figure 5. ArG, Level of the genu of the arcuate sulcus; CC, corpus callosum; CgSd, dorsal bank of the cingulate sulcus; CgSv, ventral bank of the cingulate sulcus; CgG, cingulate gyrus; CMAd, dorsal cingulate motor area; CMAr, rostral cingulate motor area; CMAv, ventral cingulate motor area; PreSMA, presupplementary motor area; SGm, medial portion of the superior frontal gyrus; SMA, supplementary motor area. Other abbreviations and conventions as in Figures 4–6.

The vast majority (93%) of the neurons labeled by transport from the spinal cord were found in 3 cortical regions (Figs. 5–9) (Table 1). Two of these regions were located in the lateral sulcus and correspond to Ig and S2 (Figs. 5, 6) (Robinson and Burton, 1980a; Mesulam and Mufson, 1982; Burton et al., 1995; Krubitzer et al., 1995). The third region was located in the cingulate sulcus at the level of the cingulate motor areas (Figs. 7, 9) (Dum and Strick, 1991, 2005; He et al., 1995).

Figure 8.

Injection site in the arm representation of the PMv. The arm representation of the PMv was identified using intracortical stimulation. Then, it was injected with a conventional tracer (diamidino yellow). A, Lateral view of the left hemisphere from a cebus monkey. The dashed box on the brain outline indicates the region magnified in B. B, Motor responses and injection site in the PMv (animal DL7). Symbols indicate the movement evoked at each site of intracortical stimulation (see key at lower right). The size of each symbol indicates the threshold (large = <10 μA, medium = 10–20 μA, small = >20 μA). A small “x” indicates each of the 3 sites of tracer injection. The spread of the tracer is indicated by the gray shading. Abbreviations and conventions as in Figures 5 and 6.

Figure 9.

Overlap between ST projections to the cingulate sulcus and the cingulate motor areas. In animal DL7, a conventional tracer (diamidino yellow) was injected into the arm representation of the PMv. In the same animal, the H129 strain of HSV1 was injected into cervical segments of the spinal cord. A, Unfolded map of neurons on the medial wall of the hemisphere that project to the PMv (the injection site is shown in Fig. 8). These neurons are located in each of the cortical motor areas in and adjacent to the cingulate sulcus, i.e., SMA, CMAr, CMAd, CMAv. A separate small patch of labeled neurons (*) is located in the caudal part of the CMAd. B, Location and density of neurons labeled in the cingulate sulcus following anterograde transneuronal transport of virus from the spinal cord. C, Overlap map of the cortical regions that project to the PMv and the cortical regions that are the target of the ST system. Blue bins indicate cortical regions containing only neurons labeled by anterograde transneuronal transport of virus from the spinal cord. Yellow bins indicate cortical regions containing only neurons labeled by retrograde transport of a conventional tracer from the PMv. Red bins indicate cortical regions containing both types of labeled neurons. The numbered arrow marks the location of the section illustrated in Figure 10. Abbreviations and conventions as in Figures 6 and 7.

Table 1.

Cortical targets of the spinothalamic tract

Lateral sulcus

Ig contained the highest density and largest number of labeled neurons (41%). These neurons were located in the posterior insula at levels just caudal to the anterior commissure and rostral to the lateral extent of the central sulcus (CS) (Fig. 5, section 328) (Fig. 6B,C). The next largest number of labeled neurons (29%) was located in a mid to rostral region of S2 Figure 6B,C). These labeled neurons were most concentrated on the dorsal bank of the lateral sulcus at levels just caudal to the lateral extent of the CS (Fig. 5, sections 456, 476) (Fig. 6B,C).

Cingulate sulcus

The labeling in the cingulate sulcus comprised 24% of the total number of labeled neurons in the cortex. These neurons were distributed in three separate groupings (Figs. 7B, 9B). A small rostral grouping (3%) was located near the fundus of the cingulate sulcus at levels just rostral to the genu of the arcuate sulcus (Fig. 5, section 160). The middle grouping was the largest (14%) and was located on the dorsal and ventral banks of the cingulate sulcus around the level of the anterior commissure (Fig. 5, section 272). A caudal grouping (8%) was located largely on the dorsal bank near the fundus of the cingulate sulcus at levels 5–6 mm caudal to the anterior commissure (Fig. 5, section 412).

No other cortical areas contained substantial numbers of labeled neurons. We did find a few small isolated patches of labeled neurons in primary somatosensory cortex (S1) (Fig. 5, sections 456, 476). Together, the number of labeled neurons in areas 3a, 3b, 1 and 2 represented ∼4% of the total cortical labeling. We also observed a few isolated single neurons labeled in several other areas (e.g., primary motor cortex, supplementary motor area, opercular area 7 and area 5). The labeling at these sites never amounted to >3 per cent of the total. It is possible that some of these neurons represent weak retrograde transport of the H129 strain (Rinaman and Schwartz, 2004). In the third animal only, a few labeled neurons were found in the ipsilateral hemisphere within the fundus of the cingulate sulcus at levels just rostral to the arcuate genu.

Spinothalamic input to the cingulate motor areas

Our prior studies have shown that the cingulate sulcus contains three distinct cingulate motor areas: rostral, dorsal and ventral (CMAr, CMAd, and CMAv) (Dum and Strick, 1991, 2005, He et al., 1995). Each of these areas projects directly to the spinal cord and to the other cortical motor areas in the frontal lobe including the primary motor cortex (M1), the dorsal premotor area (PMd) and the ventral premotor area (PMv) (Dum and Strick, 2005). Our current results raise an obvious question about the relationship between the cortical targets of the ST pathway in the cingulate sulcus and the cingulate motor areas. To examine this issue we injected the hand representation of the PMv with DY (Fig. 8) and used retrograde transport of this tracer to define the location of the hand representations of the cingulate motor areas. Then, in the same animal, we injected lower cervical segments of the spinal cord with the H129 strain of HSV1 and used anterograde transneuronal transport of the virus to define the cortical targets of the ST pathway.

Using this “double labeling” approach, we found a remarkable correspondence between the cingulate motor areas (Fig. 9A) and the targets of ST input in the cingulate sulcus (Fig. 9B,C). In particular, the small rostral grouping of ST input in the cingulate sulcus overlaps with the CMAr. Likewise, the middle grouping of ST input overlaps with both the CMAd and the CMAv (Figs. 9C, 10). The caudal grouping of ST input overlaps with a small, caudal part of the CMAd (Fig. 9C, asterisk). Our prior studies have consistently shown that this portion of the CMAd projects to both the cervical spinal cord and the hand area of M1 (Dum and Strick, 1991, 2005; He et al., 1995). Thus, the ST input from the cervical cord to the cingulate sulcus targets the arm representation in each of the cingulate motor areas.

Figure 10.

Overlap between ST projections to the cingulate sulcus and the CMAd and CMAv. Neurons labeled by anterograde transneuronal transport from the spinal cord (black dots) and neurons labeled retrograde transport from the PMv (gray dots) are plotted on a single cortical section. Abbreviations and conventions as in Figure 5.

Our observations suggest a functional linkage between the ST system and the cingulate motor areas. This led us to examine whether human studies provide evidence for a similar linkage. We performed a meta-analysis of imaging studies and compared the sites of pain-related activation with the sites of movement-related activation (Fig. 11; Table 2). Our analysis included studies that applied noxious stimuli to the hand/arm and reported significant pain-related activations on or near the midline of the contralateral hemisphere (Fig. 11, red symbols). These “pain” sites were overlapped on a map of the sites of activation associated with the performance of “simple” (blue circles) and “complex” (blue diamonds) movements [Picard and Strick (1996), taken from their Fig. 5]. The summary map (Fig. 11) also includes the outlines of the cingulate motor areas in humans.

Figure 11.

Relationship between pain-related and motor-related activations on the medial wall of the hemisphere in humans. Pain-related activations on or near the midline of the contralateral hemisphere are indicated by red symbols. The numbers in the symbols refer to the references listed in Table 2. These “pain” sites were overlapped on a map of the sites of activation associated with the performance of “simple” (blue circles) and “complex” (blue diamonds) movements [taken from Picard and Strick (1996), their Fig. 5]. The summary map also includes the outlines of the cingulate motor areas in humans (gray shading; Picard and Strick, 1996). CA-CP, Horizontal line passing through the anterior and posterior commissures; CCZ, caudal cingulate zone; RCZa, anterior subdivision of the rostral cingulate zone; RCZp, posterior subdivision of rostral cingulate zone; Vca, vertical line through the anterior commissure; Vcp, vertical line through the posterior commissure.

Table 2.

Medial wall activations associated with pain

Approximately half of the pain-related activations (Fig. 11, red circles) cluster near the Vca line and overlap the major group of activations related to simple movements (Fig. 11, blue circles). This region of overlap is within the caudal cingulate zone (CCZ), the proposed homolog of the monkey CMAd (Picard and Strick, 1996). A second, small group of pain-related activations (sites 1, 2, 5, 30) is located ∼0–10 mm rostral to the Vca line and overlaps a small group of activations related to complex movements (Fig. 11, blue diamonds). This region of overlap is within the posterior portion of the rostral cingulate zone (RCZp), the proposed homolog of the monkey CMAv (Picard and Strick, 1996). A third group of pain-related activations is located 20–25 mm rostral to the Vca line and overlaps a larger group of activations related to complex movements (Fig. 11, blue diamonds). This region of overlap is within the anterior portion of the rostral cingulate zone (RCZa), the proposed homolog of the monkey CMAr (Picard and Strick, 1996). Clearly, the human equivalents of the three cingulate motor areas also correspond to sites of pain-related activations. Thus, there is evidence in the human as well as in the monkey for a linkage between nociceptive input and the cortical control of movement. Our monkey experiments add two unique contributions to this story: the results provide (1) an anatomical substrate for the pain-related activations in the cingulate sulcus and (2) a direct demonstration of the linkage between a presumed nociceptive system and the cingulate motor areas.

Discussion

The ST system is recognized as the major route for transmitting nociceptive and thermoceptive information to the cerebral cortex (Craig, 2003). The actual cortical targets of this system have been the subject of considerable controversy (Willis et al., 2002; Craig, 2003). Anterograde transneuronal transport of the H129 strain of HSV1 enabled us to determine the precise location and relative strength of input to each of the cortical targets of the ST system. Our experiments produced two novel results. First, the major cortical targets of the ST system are Ig, S2 and several regions buried in the cingulate sulcus. Second, the ST input to the cingulate sulcus specifically targets the cingulate motor areas. This result is supported by our meta-analysis of imaging which shows that the human equivalents of the three cingulate motor areas correspond to sites of pain-related activation. These new findings have important implications for concepts about the use of ST input at the cortical level.

Insula

Over 40% of the neurons labeled by anterograde transneuronal transport of virus from the spinal cord were located in a single cortical area—Ig in the posterior insula (Fig. 12). This observation, along with prior anatomical, physiological and imaging data, lends support to the hypothesis that Ig is the primary receiving area in the cortex for nociceptive and thermoceptive information (Craig, 2003). Many ST neurons in lamina I (and some in lamina V) terminate in Po-SG (Craig, 2004; Craig and Zhang, 2006), a thalamic region which is thought to project to Ig (Mufson and Mesulam, 1984; Friedman and Murray, 1986). Most of these ST neurons are thought to transmit nociceptive and thermoceptive information (Craig, 2003). In addition, the posterior insula is the cortical region in imaging studies of human subjects that is most consistently activated by stimuli which produce acute pain (Peyron et al., 2000; Apkarian et al., 2005). Furthermore, human subjects report painful sensations following electrical stimulation of the posterior insula (Ostrowsky et al., 2002; Mazzola et al., 2006). However, the hypothesis that Ig in the posterior insula is the primary receiving area for nociceptive information lacks one critical piece of information. There is little or no evidence from physiological recordings that Ig contains a large population of neurons that are specifically sensitive to nociceptive stimuli (Schneider et al., 1993; Zhang et al., 1999). Our map of the ST input to the Ig should enable future studies to determine whether neurons in this region are especially responsive to nociceptive input.

Figure 12.

Major cortical targets of the ST system and their thalamic inputs. The percentage of the total ST input to each cortical area is indicated (“%”). This number is based on the average of two animals (DL2 and DL3, see Results for further details). The thalamic nuclei that are most likely to mediate this input are listed below each cortical area. These thalamic nuclei: (1) receive ST terminations (see Results for references), (2) contain infected neurons in the present study, and (3) are known to project to the relevant cortical area. The following references provide the basis for these thalamocortical linkages: Po-SG to Ig (Mufson and Mesulam, 1984; Friedman and Murray, 1986); VPI to S2 (Friedman and Murray, 1986; Stevens et al., 1993); MDvc to CMA (Hatanaka et al., 2003); VPLc to S1 (Gingold et al., 1991; Jones, 2007). CMA, Cingulate motor areas; MDvc, ventrocaudal portion of medial dorsal nucleus.

Although we have emphasized the potential of the insula to be the target of nociceptive information, there is also evidence that the posterior insula receives of information about temperature, itch, sensual touch, muscular and visceral sensations, vasomotor activity, hunger, thirst, and “air hunger” (Craig, 2003). For example, Olausson et al. (2002) observed activation in the posterior insula in a patient with a large fiber neuropathy when C-fibers responsive to low-threshold tactile stimuli were activated. Craig et al. (2000) found activation in a similar region associated with graded cooling stimuli. These and other observations led Craig to propose that the posterior insula represents the primary receiving area for an interoceptive system which reflects the state of internal organs and is associated with autonomic motor control (Craig, 2003). It is likely that the ST system is a major source of afferent signals for this interoceptive system.

It is important to note that many imaging studies of acute pain have reported activation not only in posterior regions comparable to Ig, but also in anterior portions of the insula (Peyron et al., 2000; Ostrowsky et al., 2002; Henderson et al., 2007). In addition, many of these studies observed bilateral activation in this region. Furthermore, recordings in S2 and Ig have found single neurons that have bilateral receptive fields (Robinson and Burton, 1980a; Schneider et al., 1993; Zhang et al., 1999). Thus, the distribution of ST input to the insula does not explain all of the patterns of activation in this region associated with acute pain. In this regard, it is important to remember that we have set the survival time to limit transneuronal transport of virus to third-order neurons. Single neuron recording and functional activation are not similarly constrained. Thus, much of the discrepancy between our anatomical results and the bilateral patterns of activation may be explained by fourth-order or longer connections.

S2

Almost 30% of the neurons labeled by anterograde transneuronal transport of virus from the spinal cord are located in another area within the lateral sulcus– S2 on the parietal operculum (upper bank of the lateral sulcus) (Fig. 12). Like the insula, S2 receives projections from thalamic nuclei that are the target of input from spinal cord neurons in laminae I and V (Fig. 12) (Friedman and Murray, 1986: Stevens et al., 1993; Craig, 2004, 2006). In meta-analyses of functional imaging studies, S2 is second only to the insula in its probability of activation by stimuli which induce acute pain (Peyron et al., 2000; Apkarian et al., 2005). Furthermore, like the posterior insula, electrical stimulation of S2 in humans can evoke painful sensations (Ostrowsky et al., 2002; Mazzola et al., 2006). Thus, our results support the view that the S2 is a second, distinct cortical site of nociceptive processing (Peyron et al., 2000; Apkarian et al., 2005).

One question raised by these results is whether Ig and S2 make differential contributions to nociceptive processing. Craig has suggested that the ST input to Ig originates largely from nociceptive and thermoceptive neurons in lamina I whereas ST input to S2 originates primarily from wide dynamic range neurons in lamina V (Craig, 2003, 2006; Craig and Zhang, 2006). On the other hand, functional imaging studies in humans have not reported clear differences in the types of stimuli that activate Ig and S2 (Peyron et al., 2000; Apkarian et al., 2005). Furthermore, recording studies in monkeys have failed to discover distinct differences between the response properties of neurons in or near Ig and S2 (Robinson and Burton, 1980b; Dong et al., 1989; Schneider et al., 1993; Zhang et al., 1999). Thus, additional information about the response properties of S2 and Ig neurons to noxious stimuli is required for any further speculation about the differential involvement of S2 and Ig in nociceptive processing.

S1

We confirmed that the various subdivisions of S1 (areas 3a, 3b, 1, 2) receive input from the ST system (Fig. 12) (Willis et al., 2002). However, our data indicate that this input is relatively weak. The number of neurons labeled in S1 by virus injections into the spinal cord is <6% of the number of neurons labeled in the lateral sulcus (Ig and S2). Consequently, our observations do not support the view that S1 is a major target of the ST system (see however, Gingold et al., 1991; Willis et al., 2002). For similar reasons, our data do not support the proposal that insular activation during imaging studies of pain processing is due to a serial flow of information from S1 and S2 to the insula (Willis et al., 2002). Instead, our results favor a direct pathway from the thalamus to the insula as the source of activation in these studies, and relegate S1 to a subordinate role in nociceptive processing.

Cingulate sulcus

The third major target of spinothalamic input (24% of the total) is cortex buried in mid regions of the cingulate sulcus (Fig. 12). This result is consistent with evidence that ST neurons in lamina I terminate in a thalamic region that projects to the cingulate sulcus (i.e., the ventrocaudal part of the medial dorsal nucleus [MDvc]) (Hatanaka et al., 2003; Craig, 2004). Also, regions of mid-cingulate cortex are consistently activated during imaging studies of acute pain (Peyron et al., 2000; Kwan et al., 2000; Büchel et al., 2002; Apkarian et al., 2005; Vogt, 2005) (Fig. 11). Painful laser stimulation evokes cortical potentials simultaneously in mid-cingulate cortex and in the S2/insula region (Frot et al., 2008). In addition, neuronal responses to noxious stimuli have been recorded in the mid-cingulate cortex in both monkeys and humans (Koyama et al., 1998; Hutchison et al., 1999). Thus, there is compelling evidence that regions of mid-cingulate cortex are the recipient of nociceptive information from the ST system.

Classically, the cingulate cortex has been considered to be part of the limbic system and to be involved in processing the affective-motivational aspects of pain (Peyron et al., 2000; Singer et al., 2004) (for review, see Vogt, 2005). Indeed, there is evidence that some activations in anterior cingulate cortex are modulated by pain unpleasantness (affect), whereas activations in somatosensory areas are generally modulated by pain intensity and localization (Rainville et al., 1997; Hofbauer et al., 2001; Kulkarni et al., 2005). On the other hand, we used a double labeling approach in monkeys to demonstrate that the ST input to the cingulate sulcus is directed solely to the cingulate motor areas. Thus, we find substantial overlap between an ascending afferent pathway involved in pain processing and the three cingulate motor areas that project both to the primary motor cortex and to the spinal cord (Dum and Strick, 1991, 1996, 2005; He et al., 1995). Furthermore, our meta-analysis showed that the human equivalents of the three cingulate motor areas correspond to sites of pain-related activation (Fig. 11). These results make it unlikely that the ST input to the cingulate sulcus is solely involved in affective-motivational aspects of pain.

Instead, several features of the cingulate motor areas reinforce the view that they are involved in motor control. The cingulate motor areas are somatotopically organized (Dum and Strick, 1991, 2005; He et al., 1995). Electrical stimulation of them in awake monkeys evokes specific limb movements (Luppino et al., 1991; Hatanaka et al., 2003), and in awake humans does not evoke reports of localized pain or unpleasant sensations (Hutchison et al., 1999). Many neurons in the cingulate motor areas, especially in the CMAd and CMAv, are well related to the preparation and execution of specific limb movements (Shima et al., 1991; Cadoret and Smith, 1997; Russo et al., 2002; Isomura et al., 2003). We have viewed the cingulate motor areas as nodes in a densely interconnected network that contributes to the generation and control of voluntary movement (Dum and Strick, 1991, 1996, 2005; Picard and Strick, 1996). This is especially the case for the CMAd and CMAv which have substantial connections with not only the primary motor cortex, but also with the PMd and PMv (Dum and Strick, 2005). Thus, ST input to the CMAd and CMAv may serve an integrative motor function which is unrelated to processing the experience of pain.

The view that ST input to the CMAd and CMAv is involved in sensorimotor integration fits with the concept that a portion of the ST system conveys information about the state of segmental interneurons involved in motor mechanisms such as spinal reflexes, locomotion and posture (Menétrey et al., 1984; Craig, 2008). Craig (2008) has proposed that a component of the ST system originates from neurons in laminae V and VII and primarily targets M1. However, our results suggest that the ST input to M1 is quite limited (see also Greenan and Strick, 1986; Stepniewska et al., 2003). Instead, the cortical motor areas that are the major target of ST input are the cingulate motor areas. The spinal laminae of origin for this input are unknown. If a component of the ST input to the CMAd and/or the CMAv originates from laminae V and VII, it could provide these areas with a corollary discharge reflecting spinal motor activity. This type of input could be used in a variety of ways including the adjustment of descending commands based on the status of spinal excitability.

The function of ST input to the CMAr may differ from that of the other cingulate motor areas. Some neurons in the CMAr show changes in activity related to attention for action and response selection based on reward value (Shima and Tanji, 1998; Akkal et al., 2002; Isomura et al., 2003; Amiez et al., 2006). The RCZa, the human equivalent of the CMAr, displays activation during tasks that involve a subject's evaluation of the consequences of their actions (Walton et al., 2004; Jocham et al., 2009). In this context, one could view ST input to the CMAr as highly salient feedback that indicates the consequences of an action and promotes adjustments in future behavior. In summary, the ST system targets multiple and diverse cortical areas and is likely to provide unique information to each area.

Footnotes

This work was supported in part by funds from the Office of Research and Development, Medical Research Service, Department of Veterans Affairs, National Institutes of Health Grants R01 NS24328 (P.L.S.), P40 RR018604 (P.L.S.), F30 NS043824 (D.J.L.), and T32 DK063922 (D.J.L.). This project was also funded, in part, under a grant with the Pennsylvania Department of Health. The Department specifically disclaims responsibility for any analyses, interpretations, or conclusions. We thank Dr. R. Dix (Georgia State University, Atlanta, GA) for supplying the H129 strain of HSV1. We also thank M. Page for the development of computer programs, P. Carras, M. O'Malley, and M. Watach for their expert technical assistance, Dr. K. Coffman for assisting in surgery, and Drs. R. Koerber and J. Roppolo for the loan of spinal stereotaxic equipment.

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