Monocytes give rise to mucosal, but not splenic, conventional dendritic cells (original) (raw)

J Exp Med. 2007 Jan 22; 204(1): 171–180.

Article

Chen Varol

1Department of Immunology and 2Department of Veterinary Resources, The Weizmann Institute of Science, 76100 Rehovot, Israel

Limor Landsman

1Department of Immunology and 2Department of Veterinary Resources, The Weizmann Institute of Science, 76100 Rehovot, Israel

Darin K. Fogg

3Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratory of Mononuclear Phagocyte Biology, Necker Enfants Malades Institute, and University of Paris Rene Descartes Medical School, Necker Enfants Malades Hospital, 75015 Paris, France

Liat Greenshtein

1Department of Immunology and 2Department of Veterinary Resources, The Weizmann Institute of Science, 76100 Rehovot, Israel

Boaz Gildor

1Department of Immunology and 2Department of Veterinary Resources, The Weizmann Institute of Science, 76100 Rehovot, Israel

Raanan Margalit

1Department of Immunology and 2Department of Veterinary Resources, The Weizmann Institute of Science, 76100 Rehovot, Israel

Vyacheslav Kalchenko

1Department of Immunology and 2Department of Veterinary Resources, The Weizmann Institute of Science, 76100 Rehovot, Israel

Frederic Geissmann

Steffen Jung

1Department of Immunology and 2Department of Veterinary Resources, The Weizmann Institute of Science, 76100 Rehovot, Israel

Received 2006 May 10; Accepted 2006 Nov 22.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

Supplementary Materials

[Supplemental Material Index]

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Abstract

The mononuclear phagocyte (MP) system is a body-wide macrophage (MΦ) and dendritic cell (DC) network, which contributes to tissue homeostasis, inflammation, and immune defense. The in vivo origins of MPs remain poorly understood. Here, we use an adoptive precursor cell transfer strategy into MP-depleted mice to establish the in vivo differentiation sequence from a recently identified MΦ/DC-restricted bone marrow (BM) precursor (MDP) via BM and blood intermediates to peripheral MΦs and DCs. We show that MDPs are in vivo precursors of BM and blood monocytes. Interestingly, grafted Gr1high “inflammatory” blood monocytes shuttle back to the BM in the absence of inflammation, convert into Gr1low monocytes, and contribute further to MP generation. The grafted monocytes give rise to DCs in the intestinal lamina propria and lung, but not to conventional CD11chigh DCs in the spleen, which develop during homeostasis from MDPs without a monocytic intermediate.

Historically, the body-wide cellular network of peripheral mononuclear phagocytes (MPs) has been subdivided into macrophages (MΦs) and DCs, which were discovered toward the end of the 19th century and in the 1970s, respectively (1, 2). Both MΦs and DCs consist of multiple subpopulations largely defined by distinct anatomic location and phenotypes. MΦs include representatives in the serosa, lamina propria (lp), lung, brain (microglia), bone (osteoclasts), and liver (Kupffer cells; reference 3). DCs, on the other hand, have been divided into epidermal Langerhans cells, plasmacytoid DCs, and conventional CD11chigh DCs, which in mice are themselves composed of three subsets (CD4+ CD8−, CD4− CD8−, and CD4− CD8+; reference 4). MPs are involved in tissue remodeling and homeostasis, as well as regulatory and stimulatory aspects of innate and adaptive immunity.

MPs arise from mesoderm-derived hematopoietic precursor cells, which in mammals are generated in two independent temporally and spatially separated waves (5). “Definitive” intra-embryonic hematopoiesis results in the generation of multipotent hematopoietic stem cells that eventually seed the BM. Certain MP populations, such as the brain microglia and epidermal Langerhans cells, are capable of self-renewal or are derived from tissue-resident precursors (6, 7). However, most other peripheral MP subsets of the adult, particularly the short-lived CD11chigh DC (8), are believed to rely on continuous replenishment from the BM-resident hematopoietic stem cells. The hematopoietic stem cell differentiation pathway into MPs includes several BM intermediates, such as the common myeloid precursors (9), granulocyte/MΦ precursors (9), and MΦ/DC precursors (MDPs; reference 10), characterized by their progressive loss of ability to give rise to other hematopoietic cell types.

Systemic dissemination into the peripheral MP pool is thought to be ensured by circulating blood monocytes (11). Human monocytes are long known to consist of discrete subpopulations (12), and, more recently, monocyte heterogeneity has also been established in the mouse (13–16) and rat (17). Circulating murine monocytes comprise two main subsets: Gr1high CX3CR1int CD62L+ CCR2+ and Gr1low CX3CR1high CCR2−. Gr1high “inflammatory” monocytes were shown to home preferentially to sites of inflammation, whereas Gr1low monocytes are believed to seed resting tissues in the steady state (15). However, the origin and biology of this intriguing short-lived leukocyte, which cannot be generated in vitro, remain poorly understood.

Here, we report the use of adoptive precursor transfer experiments in WT and MP-depleted recipient mice to study the origin, interrelation, and differentiation potential of murine BM and blood monocytes. Using intra bone cavity (IBC) transfer we establish the in vivo differentiation sequence from the recently reported MDP (10) to BM and blood monocytes and terminally differentiated peripheral MPs. Interestingly, monocytes appear to be dedicated to DC replenishment of nonlymphoid organs, such as the intestinal lp and the lung, whereas splenic DCs seem to arise from local precursors without a monocytic intermediate. Furthermore, we show that in the absence of inflammation, the Gr1high blood monocyte subset efficiently shuttles back to the BM, converts into Gr1low monocytes, and thus contributes further to MP generation.

RESULTS

MDPs give rise to BM and blood monocytes

Taking advantage of a mouse strain that carries a replacement of the CX3CR1 chemokine receptor gene by a GFP reporter (CX3CR1gfp mice; reference 18), we recently reported the isolation of a novel proliferating clonogenic precursor (termed MDP) from murine BM that gives rise exclusively to MΦs and DCs. When injected into the blood stream of recipient mice, MDPs expand and differentiate into splenic MΦs and DCs (10). In this study, we sought to study the potential of MDPs to differentiate within their native BM microenvironment and give rise to BM and blood monocytes. We therefore revised our original adoptive MDP transfer approach by injecting the MDPs into the BM, i.e., the femoral bone cavity, of the recipient mice.

The BM of CX3CR1gfp donor mice contains three main distinct CD115+ CX3CR1/GFP+ populations: CD117+ CD11b− Gr1− cells (Fig. 1 A), which represent dedicated MDPs (GFPlow; reference 10), and two discrete Gr1(Ly6C/G)high and Gr1(Ly6C/G)low subsets of CD11b+ BM monocytes (19) that in CX3CR1gfp mice appear as GFPint and GFPhigh, respectively (Fig. 1 A). To study the interrelation of these populations, we isolated BM from heterozygote mutant CX3CR1gfp donor mice (CD45.1) and sorted MDPs to purity according to surface CD117 (cKit) and GFP expression, as well as the absence of CD11b and Gr1 markers (Fig. 1 B). MDPs were then injected into the right femoral bone cavity of unmanipulated WT mice (CD45.2). The recipient mice were killed at various time points after adoptive transfer and subjected to flow cytometry analysis of blood and BM. The differential allotypic CD45 marker of host and graft, in addition to the GFP label of the CX3CR1gfp cells, allowed detection of the graft. Analysis of the recipient BM at days 3 and 6 after transfer revealed the presence of GFP+ CD45.1+ cells expressing the surface marker CD11b (Fig. 1 C). As in the donor mice, CD11b+ GFP+ cells could be further subdivided according to Gr1 and CX3CR1/GFP expression (Fig. 1 C). Interestingly, Gr1high cells, and to a lesser extent Gr1low graft-derived cells, were detected also in the noninjected (left) recipient femur (Fig. 1 D). Analysis of the blood revealed the presence of GFP+ graft-derived monocytes that were largely Gr1low (day 6; Fig. 1 E). These findings show that upon IBC transfer, MDPs are able to differentiate into both Gr1high and Gr1low BM monocytes. Given the small number of transferred MDPs (2.5 × 104 cells), this sequence likely involved proliferative expansion of the graft. To investigate the interrelation between the two BM monocyte subsets, we next isolated CD11b+ Gr1high GFPint BM monocytes from the BM of CX3CR1gfp mice (CD45.1; Fig. 2 A) and performed an IBC transfer. Analysis of recipient mice days 1 and 3 after transfer revealed the presence of graft-derived Gr1low GFPhigh BM monocytes (Fig. 2 B). These findings suggest that Gr1high BM monocytes can serve as in vivo precursors of Gr1low BM monocytes, as has been reported for Gr1high blood monocytes (16, 20). It bears mention that at both time points, graft-derived monocytes were again detected in the noninjected femura of the recipient mice (Fig. 2 C). Recipient mice also exhibited a prominent population of Gr1low blood monocytes, supporting the notion that the grafted Gr1high cells had differentiated (Fig. 2 D). Collectively, IBC transfer of MDP and BM monocytes recapitulated the sequential in vivo differentiation of MDPs, Gr1high BM monocytes, Gr1low BM monocytes, and Gr1low blood monocytes.

Characterization of CX3CR1gfp BM and IBC transfer of MDPs. (A) Flow cytometry analysis of CX3CR1gfp/+ BM. The cells in the dot plots to the right are gated according to CD115 and GFP expression. Note the presence of three main CD115+ GFP+ populations: CD11b− CD117+ MDP, CD11b+ Gr1high, and CD11b+ Gr1low BM monocytes. (B) Flow cytometry analysis of an MDP graft isolated by high speed sorting according to CD117 and GFP expression. (C) Analysis of WT recipient BM (right, injected femur) at the indicated time points after IBC transfer of 2.5 × 104 MDPs (purity: 85%, devoid of Gr1high CD11b+ cells). Note the distinct GFP intensity pattern of differentiated graft-derived (CD45.1+) CD11b+ Gr1high and Gr1low BM monocytes and the decrease in the Gr1high/Gr1low ratio with time: 14 (day 3) to 2.7 (day 6). (D) Analysis of WT recipient BM (left, noninjected femur) at the indicated time points after IBC transfer. Gating as in C. (E) Analysis of recipient blood (day 6). The cells were gated according to CD115 surface marker expression. The data are representative of at least two independent experiments per time point.

IBC transfer of BM monocytes. (A) Flow cytometry analysis of Gr1high BM monocyte graft. (B) Analysis of WT recipient BM (right, injected femur) at the indicated time points after IBC transfer of 5 × 105 Gr1high BM monocytes (purity: 90%). Note the distinct GFP intensity pattern of differentiated graft-derived (CD45.1+) CD11b+ Gr1high and Gr1low cells and the decrease in the Gr1high/Gr1low ratio with time: 5 (day 1) to 1.8 (day 3). (C) Analysis of WT recipient BM (left, noninjected femur) at the indicated time points after IBC transfer. Gating as in C. The data are representative of at least two independent experiments per time point. (D) Analysis of recipient blood (day 3). Note the sizeable presence of graft-derived (CD45.1+) CD11b+ Gr1low monocytes.

Gr1high inflammatory monocytes shuttle between the blood and BM

The detection of graft-derived BM monocytes in the noninjected femur after the IBC transfer of MDPs and BM monocytes (Figs. 1 D and 2 C) suggested that grafted cells or their descendants had entered the circulation and shuttled between bone cavities. Furthermore, the rare graft-derived cells in the noninjected femura were mainly of the Gr1high phenotype. We previously reported that homing of the Gr1high CD62L+ CCR2+ monocyte subset was restricted to sites of inflammation. In the absence of the latter, adoptively transferred Gr1high blood monocytes, but not Gr1low blood monocytes, rapidly disappeared from the circulation of recipient mice (15). Our present results raised the possibility that this observation might have been due to the immediate and quantitative recruitment of the Gr1high blood monocytes to the recipient BM. To investigate this issue, we adoptively transferred Gr1high blood monocytes isolated by MACS from Rag−/− CX3CR1gfp mice (Fig. 3 A) to the circulation of WT recipients. Analysis of the recipient mice on day 4 revealed the abundant presence of grafted GFP+ cells in the recipient BM (Fig. 3 B), which were Gr1high and Gr1low (not depicted). In contrast and as previously reported (15), we failed to detect Gr1high monocytes in the recipient blood, although it contained a sizeable population of Gr1low monocytes (Fig. 3 B). This indicates that in the absence of inflammation, Gr1high blood monocytes efficiently shuttle from the blood to the BM. These data also directly support the notion that Gr1high blood monocytes are in vivo precursors of Gr1low monocytes (16, 20). To further substantiate this point, we i.v. transferred Gr1high BM monocytes into recipient mice. Like the Gr1high blood monocytes, these cells rapidly homed to the recipient BM. Day 1 after transfer, graft-derived monocytes were mainly Gr1high in both the BM and blood compartments, whereas at day 3, the grafted cells had almost quantitatively converted into Gr1low monocytes (Fig. 3 C and Table I). To confirm the BM homing of the i.v. injected Gr1high BM monocytes, we performed whole body optical imaging using the near-infrared lipophilic carbocyanine tracer DiR (21). Although most transferred DiR-labeled monocytes accumulated in the lung, liver, and spleen, grafted cells were also readily detectable in the BM (femora and cranium) of the recipient mice (Fig. 4, A–C). Independent intravital experiments with CFSE-labeled grafts suggested that Gr1high monocytes had indeed extravasated and entered the cranial BM parenchyma (Fig. 4 D). These results show that Gr1high monocytes efficiently home to the BM. However, unlike senescent neutrophils that return to the BM to die (22), Gr1high monocytes recycle, differentiate into Gr1low monocytes, and can return to the blood to further contribute to the peripheral MP pool.

Gr1high blood and BM monocytes shuttle between blood and BM. (A) Flow cytometry analysis of a blood monocyte graft isolated from Rag−/− CX3CR1gfp mice. The GFP− cells are Ly6G+ CX3CR1− granulocytes (reference 18). (B) Analysis of recipient BM and blood 4 d after adoptive cell transfer of Gr1high blood monocytes (105 cells). Note the presence of graft-derived (GFP+) Gr1low monocytes in the blood. Fractions of graft-derived GFP+ cells of total CD115+ cells in recipient BM (0.12%) and in recipient blood (0.76%). (C) Analysis of BM and blood of WT recipients of Gr1high BM monocytes (5 × 105 cells; purity: 96%) days 1 and 3 after i.v. transfer. Note the loss of Ly6C/G expression on grafted Gr1high BM monocytes. Bar diagrams summarize data obtained from three mice per time point. Note the difference of GFP intensity of BM and blood monocytes on day 1 (mean fluorescence intensity: 274.75 ± 13.9 vs. 376.6 ± 20.5; P = 0.003). (D) Flow cytometry analysis of BM and peritoneal cavity (PC) lavage of WT recipients of MACS-purified CD115+ BM monocytes (106 cells; purity: 88%) that were left untreated or had been inoculated with thioglycollate. Note the recruitment of grafted Gr1high BM monocytes to the inflamed peritoneal cavity. The data are representative of at least two independent experiments per time point.

Table I.

Distribution of adoptively transferred Gr1high BM monocyte grafts in the BM and blood compartment of recipient mice

Total no. CD115+ cells	% graft-derivedCD115+ cells	No. graft-derivedCD115+ cells
Day 1
Blood	1.6 × 105 a	1.6 (± 0.15)	2.6 × 103
BM	5 × 107 b	0.17 (± 0.01)	8.5 × 104
Day 3
Blood	0.6 (± 0.13)	103
BM	0.05 (± 0.01)	2.3 × 104
Day 6
Blood	0.15 (± 0.03)	240
BM	0.033 (± 0.005)	1.6 × 104

Whole body imaging of Gr1high BM monocyte recipients. (A) Color-coded near-infrared fluorescent image overlaid on a photographic image of a CD1 nude mouse 16 h after tail vein injection of DiR-labeled BM monocytes (3 × 106 cells; purity: 94%; right). Control (acquired on IVIS 100) is shown on the left. Note the presence of label in femura. (B) Color-coded near-infrared fluorescent image overlaid on a photographic image of isolated lung, spleen, and femura of CD1 nude recipients of DiR-labeled BM monocytes (right) and noninjected control (left; 3 × 106 cells; purity: 94%). (C) Monochrome fluorescent microscopy image of a cranium of a CD1 nude mouse that received DiR-labeled BM monocytes (3 × 106 cells; purity: 94%). Note the presence of labeled cells in bone cavities. (D) Dual-channel fluorescent microscopic image of a cranium of a C57BL/6 WT recipient mouse that had received an i.v. injection of CFSE-labeled BM monocytes (3 × 106 cells; purity: 96%). Arrows indicate two graft-derived cells that extravasated into the BM parenchyma. Bar, 100 μm. The data are representative of two independent experiments.

Collectively, our results suggest that Gr1high BM and blood monocyte populations are linked. To investigate a potential functional overlap between these two populations, we tested Gr1high BM monocytes for a hallmark of their blood counterpart, e.g., the recruitment to sites of inflammation (13, 15, 23). Highlighting the dual homing potential of i.v. injected Gr1high BM monocytes, thioglycollate-induced inflammation caused the grafted cells to deviate from their steady-state route (BM) to the peritoneal cavity (Fig. 3 D). This indicates further that Gr1high BM and blood monocyte subsets have functional similarities.

MDPs, but not monocytes, can replenish splenic DCs

Upon i.v. transfer into irradiated and young nonirradiated recipient mice, MDPs give rise to splenic DCs (10), which are characterized by a frequent steady-state turnover (8). However, MDP differentiation into long-lived MPs, particularly of peripheral tissues, could have been hampered by the limited precursor input in the time window analyzed (10). We therefore sought to improve the engraftment of the MP compartments by depleting DCs and MΦs from the recipients before MDP transfer. To this end, we took advantage of CD11c–diphtheria toxin (DTx) receptor (DTR) transgenic mice, which harbor CD11c-expressing MPs, including MΦs and DCs that are sensitive to DTx and are hence depleted upon DTx treatment (24–27). In this conditional cell ablation system, targeted cells die by apoptosis and are removed without causing major inflammation (28). Their replenishment might therefore mimic steady-state conditions. BM chimeras generated by reconstitution of lethally irradiated WT mice with CD11c-DTR transgenic BM allow for extended MP depletion (29, 30). MDPs were isolated from CX3CR1gfp donor mice (CD45.1) and injected into DTx-treated BM chimeras. Analysis of the recipient 7 d after i.v. MDP transfer revealed the presence of graft-derived Gr1high and Gr1low monocytes in the BM and blood (not depicted). At the same time, the spleen of the MDP recipient mouse was efficiently seeded by DTx-resistant CD11chigh DCs, including CD11b+ and CD11b− cells (Fig. 5 A). As in CX3CR1gfp donor mice (10, 18), CD11c+ CD11b− (CD8α+) DCs could be subdivided into CX3CR1/GFP+ and CX3CR1/GFP− subsets. In addition, recipient spleens contained an appreciable population of CD11b+ CD11cint cells of unknown identity that were also identified in donor spleens. Because MDPs are in vivo precursors of Gr1high BM monocytes (Fig. 1 C), we next investigated the potential of the monocytes to give rise to splenic DCs in the MP-depleted recipients. Interestingly, grafted Gr1high BM monocytes failed to give rise to CD11chigh splenic DCs but differentiated almost exclusively into the CD11b+ CD11cint population we had previously observed with the MDP graft (Fig. 5 A). Because MDPs are monocyte precursors but the latter do not generate splenic CD11chigh DCs, we next examined whether MDPs could give rise to splenic DCs without prior differentiation into monocytes, i.e., when placed directly in the spleen. As seen in Fig. 5 B, intra-splenic injection of BM MDPs into MP-depleted mice yielded CD11b+ and CD11b− CD11chigh DCs. This suggests that local spleen-resident precursors, such as MDPs, might replenish the CD11chigh DC population without a monocytic intermediate.

MDPs, but not monocytes, act as precursors of splenic DCs. (A) Flow cytometry analysis of CX3CR1gfp donor spleen and spleens of DTx-treated, MP-depleted mice with and without engraftment of CX3CR1gfp MDP (8 × 104 cKit+ CD115+ Gr1− CD11b− cells; purity: 80%) and Gr1high BM monocytes (8 × 105 CD115+ Gr1+ CD11b+ cells; purity: 88%). Note the presence of graft-derived (CD45.1+) DCs (CD11chigh) in MDP recipients that, as in donor mice CD11b− DCs, are split into CX3CR1/GFP+ and CX3CR1/GFP− cells. CD11chigh/CD11cint ratios in an MDP recipient: 2.7 in Gr1high monocyte recipient 0.05 (as determined by the indicated gates). (B) Flow cytometry analysis of the spleen of an MP-depleted mouse with and without intra-splenic injection of CX3CR1gfp BM MDPs (2.5 × 104 cells; purity: 96%). Note the presence of graft-derived (CD45.1+) DCs (CD11chigh) in MDP recipients. The data are representative of two independent experiments.

Monocytes replenish intestinal lp and lung DCs

Because monocytes are established DC precursors (3), we decided to investigate whether the grafted Gr1high BM monocytes can give rise to DCs in other nonlymphoid tissues. We first chose to study the seeding of the intestinal lp, which, as we recently reported, contains two discrete populations of CX3CR1+ lpDCs and CX3CR1− lpMΦs (27, 31). Both of these cell types are CD11c+ and hence DTx sensitive in CD11c-DTR transgenic mice and [DTR→WT] mixed BM chimeras (27, 29). When transferred into untreated WT mice, Gr1high BM monocytes essentially failed to give rise to lpMP, as indicated by the absence of CD11c+ CD45.1+ cells (Fig. 6 A). DTx-induced lpMP depletion in the [DTR→WT] recipients, however, promoted the efficient seeding of this peripheral tissue with graft-derived (CD45.1+) CX3CR1/GFP+ lpDCs and CX3CR1/GFP− lpMΦs (Fig. 6 A). lpMPs were also observed after the adoptive transfer of MDPs (Fig. 6 A). To address the potential role of inflammatory signals associated with our conditional ablation strategy in graft recruitment and differentiation, we repeated the experiment using a novel strain of mice that constitutively lacks CD11c+ lpMPs and was recently developed in our laboratory (to be described elsewhere). Flow cytometry and histological analysis of these recipient mice showed efficient engraftment of their lp with CD45.1+ CX3CR1/GFP+ lpDCs and CX3CR1/GFP− lpMΦs (Fig. 6, A and B), arguing in favor of the homeostatic nature of this MP differentiation route. However, further experimentation will be needed to assess the contribution of the MΦ/DC depletion to the process. Notably, recruitment could also be mediated by constitutively expressed inflammatory signals present at the mucosal tissues. Further clarification of this issue will require the definition of the molecular parameters that guide the transferred cells to the lp. To extend our analysis to another nonlymphoid organ, we studied the lung parenchyma of CD11c-DTR transgenic recipient mice that were depleted of lung DCs by intra-tracheal DTx instillation (26). Grafted Gr1high BM monocytes gave efficient rise to lung DCs, which can be defined by coexpression of the CD11c and CD11b integrins, as well as of CX3CR1 (32, 33, 56; Fig. 6 C).

Monocytes are precursors of MPs in the intestine and lung. (A) Flow cytometry analysis of small intestinal lp cells isolated from a CX3CR1gfp donor mouse, as well as of WT, MP-depleted, and MP-deficient recipients of CX3CR1gfp Gr1high BM monocytes and MDPs (7 d after i.v. transfer of 8 × 105 CD115+ CD11b+ Gr1+ monocytes [purity: 88%] or 8 × 104 CD117+ Gr1− CD11b− MDPs [purity: 80%]). Note the presence of graft-derived (CD45.1+) CD11c+ CX3CR1/GFP+ lpDCs and CD11c+ CX3CR1/GFP− lpMΦs in donors and MP-depleted and MP-deficient recipients of precursor grafts. The data are representative of three independent experiments. (B) Fluorescence microscopy analysis of small intestinal villi of mice constitutively lacking lpDCs and lpMΦs with or without adoptive transfer of CX3CR1gfp BM monocytes (106 CD115+ Gr1high CD11b+ cells; purity: 85%). Note the presence of graft-derived CX3CR1/GFP+ lpDCs in the live tissue of a BM monocyte recipient. Bar, 100 μm. The data are representative of three independent experiments. (C) Flow cytometry analysis of parenchymal lung cells isolated from CX3CR1gfp donor mouse, an MP-depleted mouse, and MP-depleted recipients of CX3CR1gfp Gr1high BM monocytes (7 d after i.v. transfer of 7.5 × 105 CD115+ Gr1+ CD11b+ cells; purity: 85%). Note the presence of graft-derived (CD45.1+) CD11c+ CD11b+ CX3CR1/GFP+ lung DCs in the graft recipient. The data are representative of two independent experiments.

Collectively, adoptively transferred monocytes fail to give rise to CD11chigh DCs in the spleen but efficiently do so in the intestinal lp and lung. These results suggest a differential contribution of monocytes and MDPs to the lymphoid and nonlymphoid DC compartment.

DISCUSSION

Here, we report three novel findings on the origin, interrelation, and fate of murine MPs. First, we establish that a recently reported MDP (10) differentiates in vivo into BM and blood monocytes. Second, we show that in the absence of inflammation, the previously reported Gr1high inflammatory monocyte subset (15) homes back to the BM, recycles, and takes further part in MP differentiation. Third, and most importantly, our data together with other recent studies (10, 34–36) establish that the spleen relies on a distinct nonmonocytic DC precursor input. In contrast, nonlymphoid tissues including the intestinal lp, lung, and epidermis—possibly most peripheral tissues—are seeded by monocytes for renewal of DCs. At least in some cases, however, the role of monocytes in reseeding tissue DCs is subordinate to local self-renewal (7).

Maintenance of most peripheral MPs is believed to require continuous replenishment from the BM. This includes the short-lived DC compartment (37), but also MΦs. However, the latter (38–40) and, more recently, splenic DCs (34, 41) were also reported to be capable of limited self-renewal. The link between the BM and peripheral MΦs or DCs is thought to be provided by circulating blood monocytes (Fig. S1, available at http://www.jem.org/cgi/content/full/jem.20061011/DC1). Despite this critical position in the MP differentiation pathway, the in vivo biology of this ephemeral MP intermediate (13, 15, 16, 35, 42, 43) remains poorly understood. Based on the expression of the chemokine receptors CX3CR1 and CCR2, human and murine peripheral blood monocytes can be subdivided into two major populations (11). Furthermore, studies in the mouse suggested that these subsets are functionally distinguishable in that inflammatory CX3CR1low CCR2+ Gr1 (Ly6C/G)high monocytes are preferentially recruited to sites of tissue damage, whereas CX3CR1high Gr1(Ly6C/G)low cells extravasate spontaneously to resting tissues (15). Beyond this dichotomy, “monocytes” are likely to include additional minor subpopulations, as suggested by the recent identification of the Ly6Cmid CCR7+ CCR8+ subset (16, 20), as well as subsets with novel pro-angiogeneic activities (44, 45).

Monocytes are known to originate in the BM from a granulocyte-myeloid precursor (9). More recently, we identified a BM-resident clonotypic MP progenitor that, as opposed to a granulocyte-myeloid precursor, lost the potential to differentiate into granulocytes. Instead, when cultured under the appropriate in vitro conditions or transferred into recipient mice, the CX3CR1+ CD117+ lin− cells expanded and gave rise exclusively to MΦs and DCs. They were hence named MDPs for MΦ/DC precursors (10). We now investigated the potential of MDPs to differentiate within their native BM microenvironment. Using IBC transfer, we showed that the CD11b− Gr1 (Ly6C/G)− MDPs differentiate into CD11b+ Gr1(Ly6C/G)high and CD11b+ Gr1(Ly6C/G)low BM monocytes, which have been previously reported and are potentially able to differentiate into MPs (46, 47). The IBC transfer also allowed the detection of graft-derived peripheral blood monocytes. These data, together with the results obtained from subsequent IBC transfers of the Gr1high BM monocyte intermediate, document a sequence of myeloid differentiation from a BM-resident precursor via BM-resident intermediates to circulating blood monocytes (Fig. S1).

In most current schemes of myeloid differentiation, the blood monocyte appears as a “one-way intermediate” from the BM to the periphery (11). A most intriguing finding of our studies is, however, that blood monocytes efficiently home back to the BM (Fig. S1). Thus, after IBC transfer of MDPs, we unexpectedly retrieved graft-derived cells from the noninjected contra-lateral bone. Using IBC and i.v. engraftment of Gr1high BM monocytes, we directly showed that this cell population is responsible for the inter-BM translocation. The i.v. transfer of blood monocytes further revealed that the BM homing potential is inherent to all Gr1high monocytes. We previously reported that Gr1high blood monocytes are dedicated to migrate to sites of inflammation but in the absence of the latter disappeared from the circulation of recipient mice (15). Our present results strongly suggest that the grafted Gr1high inflammatory monocytes in these studies had shuttled back to the BM. Furthermore, they might also provide an explanation for the reported loss of grafted monocytes in other studies (16). Importantly, Gr1high BM and blood monocytes that returned to the BM converted into Gr1low monocytes and contributed further to MP generation (see below). Peripheral blood monocyte shuttling to the BM likely plays a role in antigen acquisition (43). Furthermore, it will have to be taken in consideration when interpreting the results of parabiosis experiments. Murine blood monocytes ingest Listeria monocytogenes upon in vivo challenge and critically contribute to dissemination of the bacteria to the brain (48). Moreover, circulating phagocytes have been implied in the systemic spreading of Salmonella typhimurium after oral challenge (49). Blood monocyte shuttling to the BM could be exploited by pathogens to reach this immuno-privileged compartment and establish persistent infection or latency.

Sunderkotter et al. (16) reported that Ly6Chigh blood monocytes differentiate into Ly6Clow monocytes. Together with the identification of a Ly6Cmid CCR7+ CCR8+ monocyte subset in the blood, this led to the suggestion that monocyte conversion occurs within the circulation (16, 20). Our Gr1 (Ly6C/G)high BM and blood monocyte transfer studies provide further direct evidence that this monocyte subset acts as an efficient precursor pool of Gr1low BM and blood monocytes. However, given the kinetics of the BM monocyte subset appearance after IBC transfer and the efficient BM homing of the i.v injected Gr1high monocytes, our results suggest that the BM is a major site of monocyte conversion. This could also explain the reported transient loss of fluorochrome-labeled Ly6Chigh blood monocytes and their reappearance as Ly6Clow cells (16).

We recently noted that adoptively transferred monocytes fail to differentiate into splenic DCs (10). We have now substantiated this observation by using the conditional depletion of splenic host DCs, thereby boosting replenishment of the DC compartment by grafted cells. Strikingly, adoptively transferred monocytes efficiently seeded the recipient spleen with CD11cint progenies but did not give rise to splenic CD11chigh DCs. In contrast, in the same MP-depleted mice, the grafted Gr1high monocytes efficiently reconstituted the small intestinal lp with lpDCs and lpMΦs (27). Furthermore, grafted Gr1high monocytes also gave rise to DCs in another nonlymphoid tissue, the lung. Importantly, the conversion of Gr1high monocytes into Gr1low cells precludes drawing the conclusion that the Gr1high monocyte subset is the direct precursor of the intestinal and lung DCs in the present study. Notably, Yrlid et al. (17) recently reported that in the rat, the CCR2neg monocyte subset has the potential to differentiate into intestinal lymph DCs (17), and we observed the generation of lung DCs from adoptively transferred fractionated Gr1low blood monocytes (unpublished data).

Recent studies indicate that steady-state DC maintenance in lymphoid organs might be independent of monocyte input, and that spleen DCs have the potential for self-renewal or rely on local precursors (34, 41). The i.v. and intra-splenic transfer of MDPs shows that BM MDPs can give rise to splenic CD11chigh DCs, including both CD11b+ and CD11b− cells. These findings suggest that spleen-resident precursors might give rise to steady-state DCs in the spleen without a monocytic intermediate. Interestingly, such a cell with DC differentiation potential has recently been reported by Naik et al. (36), and it will be important to establish the link of these so-called “pre-cDCs” to the MDP.

The heterogeneity of the body-wide MΦ/DC network and the emerging differential in vivo functions of distinct MΦ/DC subsets (24, 50) suggest that manipulation of the MP system might be of therapeutic value. Rather than transferring terminally differentiated MPs, which cannot faithfully be generated in vitro and are unlikely to reach the desired physiological microenvironment, MP system manipulation could rely on precursor differentiation in their physiological context. Such strategies will, however, require an in-depth understanding of the underlying MP differentiation pathways. In our study, we have established two sequences of in vivo MP differentiation using recipient mice in which we conditionally ablated defined MPs in lymphoid and nonlymphoid organs. We show that adoptively transferred MDPs (10) can reconstitute DCs in the spleen and intestinal lp, whereas engraftment with monocytes allows efficient seeding of nonlymphoid tissues with MΦs and DCs. Importantly, grafted Gr1 (Ly6C/G)high monocytes efficiently home to the BM and enter physiological differentiation pathways. Combined with the recent progress in our understanding of the molecular checkpoints of MP precursor differentiation (51–54), genetically modified precursor grafts might allow the development of strategies for the manipulation of the peripheral MP pool.

MATERIALS AND METHODS

Animals.

This study involved the use of WT, heterozygote mutant CX3CR1+/gfp (18), and CD11c-DTR transgenic mice (B6.FVB-Tg [Itgax-DTR/GFP] 57Lan/J; reference 24), all of which were backcrossed against a C57BL/6 background. All whole body imaging was performed on CD1 nude recipients. Recipient mice that constitutively lack CD11chigh MPs will be described elsewhere. Mixed [DTR→WT] BM chimeras for conditional MP ablation were generated as reported previously (29). In brief, C57BL/6 WT mice were exposed to a single lethal dose of 950 rad total body irradiation, followed by i.v. transfer of 5 × 106 CD11c-DTR transgenic BM cells. The mice were allowed to rest for 8 wk before use. For systemic MP depletion, DTR transgenic BM chimeras were inoculated intraperitoneally every other day with DTx (D-2918; Sigma-Aldrich) at 8 ng/g body weight (29). For pulmonary MP depletion, we intratracheally installed 100 ng DTx (26 and unpublished data). Peritoneal sterile inflammation was induced by injection of thioglycollate (Diffco). All mice were maintained under specific pathogen-free conditions and handled according to protocols approved by the Weizmann Institute Animal Care Committee as per international guidelines.

Isolation of MDP and BM monocyte precursor grafts.

BM cells were harvested from the femora and tibiae of CX3CR1GFP/+ CD45.1 mice and enriched for mononuclear cells on a Ficoll density gradient. The cells were then immunostained with anti–CD117-PE, anti–CD11b-PECy7/PerCP, and anti–Gr1-APC fluorochrome–conjugated antibodies. MDP cells were identified as CX3CR1 (GFP), CD117 (cKit) positive cells negative for CD11b and Gr1 markers. These cells were purified by high speed sorting using a FACS Aria (Becton Dickinson) and injected i.v. into congenic CD45.2 WT mice or DTx-treated mixed [DTR→WT] BM chimeras. Gr1high BM monocytes were isolated by high speed sorting of the Gr1highCD11b+CD115+ BM cell fraction. For IVIS and intravital microscopy experiments, the monocyte graft was isolated through MACS enrichment using biotinylated anti-CD115 antibodies and streptavidin-coupled magnetic beads (Miltenyi Biotec).

Adoptive transfer procedure.

If not indicated otherwise, 0.2 ml PBS containing the respective cell populations was injected into the tail vein. For spleen and IBC transfer, mice were anesthetized with a mixture containing 15% xylazin (20 mg/ml; Vitamed) and 85% ketaset (100 mg/ml; Fort Dodge Animal Health). Cells were injected directly into the spleen (20 μl vol) or into the BM cavity (∼5 μl vol) using a U-100 insulin syringe (with a 30-G needle; Becton Dickinson). For IBC transfer, the knee side of the femur was pierced in advance with a 27-G 1/2 needle.

Analysis of recipient mice and flow cytometry.

lp cells were isolated as described previously (27). Fluorochrome-labeled monoclonal antibodies were purchased from BD Biosciences or eBioscience and used according to the manufacturer's instructions. Cells were analyzed with a FACSCalibur cytometer (Becton Dickinson) using CELLQuest software (Becton Dickinson).

Whole body and fluorescent imaging.

Isolated monocytes were labeled ex vivo with the near infrared lipophilic carbocyanine dye 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide (DiR; Invitrogen) for 1 h (21). Labeled cells were injected i.v. into CD1 nude mice. DiR-labeled monocyte localization within the intact animal, as well as isolated within organs, was assessed using the IVIS 100 Series Imaging System (Xenogen). The excitation (Ex) and emission (Em) filter sets were 710–760 and 810–760 nm, respectively. For intravital microscopy imaging, monocytes were labeled with the intracellular fluorescent dye, CFSE (Invitrogen). Monocytes in the cranium were visualized using a Zoom Stereo Microscope SZX-RFL-2 (Olympus) equipped with a fluorescence illuminator and a CCD camera Pixelfly QE (PCO). The Ex and Em filter for fluorescence in the visual spectrum were: Ex 460–490/ Em 510–550 nm (green channel) and Ex 520–550 nm/Em 580–630 nm (red channel). The Ex 710–750/Em 780–830 nm filter set was used for fluorescence in the near-infrared spectrum. Images were acquired using camera-controlling software (Camware; PCO) with ImageJ 1.330 software.

Supplemental Material

[Supplemental Material Index]

Acknowledgments

We thank Drs. R. Alon, Y. Pewzner-Jung, and A. Mahler for critical reading of the manuscript; R. Krauthgamer, E. Ariel, and A. Sharp for help with flow cytometry; and Y. Chermesh and O. Amram for animal husbandry.

This work was supported by the MINERVA Foundation, the Israel Science Foundation, and the EU6 Program for Migration and Inflammation Network. S. Jung is the incumbent of the Pauline Recanati Career Development Chair and a Scholar of the Benoziyo Center for Molecular Medicine. F. Geissmann is supported by INSERM, the City of Paris, the FRM, the Fondation de France, and the Fondation Schlumberger.

The authors have no conflicting financial interests.

Notes

Abbreviations used: DTR, diphtheria toxin receptor; DTx, diphtheria toxin; IBC, intra bone cavity; lp, lamina propria; MDP, MΦ/DC precursor; MP, mononuclear phagocyte; MΦ, macrophage.

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