High-fructose, medium chain trans fat diet induces liver fibrosis and elevates plasma coenzyme Q9 in a novel murine model of obesity and nonalcoholic steatohepatitis - PubMed (original) (raw)
Michelle Kirby, Stavra A Xanthakos, Samir Softic, Ariel E Feldstein, Vijay Saxena, Peter H Tang, Lili Miles, Michael V Miles, William F Balistreri, Stephen C Woods, Randy J Seeley
Affiliations
- PMID: 20607689
- PMCID: PMC2932817
- DOI: 10.1002/hep.23797
High-fructose, medium chain trans fat diet induces liver fibrosis and elevates plasma coenzyme Q9 in a novel murine model of obesity and nonalcoholic steatohepatitis
Rohit Kohli et al. Hepatology. 2010 Sep.
Abstract
Diets high in saturated fat and fructose have been implicated in the development of obesity and nonalcoholic steatohepatitis (NASH) in humans. We hypothesized that mice exposed to a similar diet would develop NASH with fibrosis associated with increased hepatic oxidative stress that would be further reflected by increased plasma levels of the respiratory chain component, oxidized coenzyme Q9 ((ox)CoQ9). Adult male C57Bl/6 mice were randomly assigned to chow, high-fat (HF), or high-fat high-carbohydrate (HFHC) diets for 16 weeks. The chow and HF mice had free access to pure water, whereas the HFHC group received water with 55% fructose and 45% sucrose (wt/vol). The HFHC and HF groups had increased body weight, body fat mass, fasting glucose, and were insulin-resistant compared with chow mice. HF and HFHC consumed similar calories. Hepatic triglyceride content, plasma alanine aminotransferase, and liver weight were significantly increased in HF and HFHC mice compared with chow mice. Plasma cholesterol (P < 0.001), histological hepatic fibrosis, liver hydroxyproline content (P = 0.006), collagen 1 messenger RNA (P = 0.003), CD11b-F4/80+Gr1+ monocytes (P < 0.0001), transforming growth factor beta1 mRNA (P = 0.04), and alpha-smooth muscle actin messenger RNA (P = 0.001) levels were significantly increased in HFHC mice. Hepatic oxidative stress, as indicated by liver superoxide expression (P = 0.002), 4-hydroxynonenal, and plasma (ox)CoQ9 (P < 0.001) levels, was highest in HFHC mice.
Conclusion: These findings demonstrate that nongenetically modified mice maintained on an HFHC diet in addition to developing obesity have increased hepatic ROS and a NASH-like phenotype with significant fibrosis. Plasma (ox)CoQ9 correlated with fibrosis progression. The mechanism of fibrosis may involve fructose inducing increased ROS associated with CD11b+F4/80+Gr1+ hepatic macrophage aggregation, resulting in transforming growth factor beta1-signaled collagen deposition and histologically visible hepatic fibrosis.
Figures
Comment in
- Fructose at the center of necroinflammation and fibrosis in nonalcoholic steatohepatitis.
Alisi A, Manco M, Pezzullo M, Nobili V. Alisi A, et al. Hepatology. 2011 Jan;53(1):372-3. doi: 10.1002/hep.23873. Epub 2010 Jul 29. Hepatology. 2011. PMID: 20814895 No abstract available. - Non-alcoholic fatty liver disease and fructose: bad for us, better for mice.
Anania FA. Anania FA. J Hepatol. 2011 Jul;55(1):218-20. doi: 10.1016/j.jhep.2011.01.029. Epub 2011 Feb 19. J Hepatol. 2011. PMID: 21338637 Free PMC article. No abstract available. - No transfats.
Neuschwander-Tetri BA. Neuschwander-Tetri BA. Hepatology. 2011 Aug;54(2):750-1. doi: 10.1002/hep.24321. Epub 2011 Jun 27. Hepatology. 2011. PMID: 21437925 No abstract available. - Dietary supplementation with methyl donor groups could prevent nonalcoholic fatty liver.
Cordero P, Campion J, Milagro FI, Martínez JA. Cordero P, et al. Hepatology. 2011 Jun;53(6):2151-2. doi: 10.1002/hep.24164. Epub 2011 Apr 29. Hepatology. 2011. PMID: 21538430 No abstract available.
References
- Bray GA, Nielsen SJ, Popkin BM. Consumption of high-fructose corn syrup in beverages may play a role in the epidemic of obesity. Am J Clin Nutr. 2004;79:537–543. - PubMed
- Cave M, Deaciuc I, Mendez C, Song Z, Joshi-Barve S, Barve S, McClain C. Nonalcoholic fatty liver disease: predisposing factors and the role of nutrition. J Nutr Biochem. 2007;18:184–195. - PubMed
- Ekstedt M, Franzen LE, Mathiesen UL, Thorelius L, Holmqvist M, Bodemar G, Kechagias S. Long-term follow-up of patients with NAFLD and elevated liver enzymes. Hepatology. 2006;44:865–873. - PubMed
- McCullough AJ. The clinical features, diagnosis and natural history of nonalcoholic fatty liver disease. Clin Liver Dis. 2004;8:521–533. viii. - PubMed
- Day CP. From fat to inflammation. Gastroenterology. 2006;130:207–210. - PubMed
Publication types
MeSH terms
Substances
Grants and funding
- R01 DK017844/DK/NIDDK NIH HHS/United States
- P30 DK078392/DK/NIDDK NIH HHS/United States
- K08 DK084310-01/DK/NIDDK NIH HHS/United States
- K08 DK084310/DK/NIDDK NIH HHS/United States
- K12 HD028827/HD/NICHD NIH HHS/United States
- 1K08DK084310/DK/NIDDK NIH HHS/United States
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Research Materials
Miscellaneous