Dr T . Ananthi | Bharthidasan, S.T.E.T Women's College, Mannargudi (original) (raw)

Papers by Dr T . Ananthi

Research paper thumbnail of HPTLC, FTIR AND GC-MS ANALYSIS OF ETHANOL EXTRACT OF Talinum triangulare (Jacq.) Willd

Annals of Roman Society and Cell Biology, 2021

Medicinal plants are the important bio resources of drugs for traditional system of medicine. The... more Medicinal plants are the important bio resources of drugs for traditional system of medicine. They comprise of various natural dynamic fixings; in this manner they are utilized for the treatment of an extensive number of irresistible sicknesses. The aim of the present study to identify the phytoconstituents from the Talinum triangulare leaves using various techniques such as FTIR, HPTLC and GC-MS analysis.

Research paper thumbnail of Physicochemical, phytochemicals and antioxidant activity of leaves of Sesbania grandiflora (L

The antioxidant activity of aqueous leaf extracts of Sesbania grandiflora was investigated using ... more The antioxidant activity of aqueous leaf extracts of Sesbania grandiflora was investigated using the DPPH radical scavenging assay, Hydrogen peroxide assay and Total antioxidant activity. In all testing a positive correlation existed between concentration of the extract and percentage inhibition of free radicals. Phytochemical screening, proximate analysis, mineral analysis by XRF were also determined. Qualitative screening for phytochemical showed the presence of alkaloids, flavonoids, phenols, tannins, terpenoids and cardiac glycosides. Proximate and mineral analysis showed moisture content (75.4%), ash content (2.24%), crude fibre (15.77%), crude lipid (1.81%), protein (7.14%), carbohydrate (12.8%) and minerals namely sodium, potassium and calcium which highlighted its good nutritional value. The results concluded that the presence of phytochemicals and some minerals proves that it is really an antioxidative.

Research paper thumbnail of HPTLC analysis and anti-inϐlammatory activity of Plectranthus amboinicus (Lour.) leaves

International Journal of Research in Pharmaceutical Sciences, 2021

Plectranthus amboinicus (Lour.) Spreng. are generally used in Chinese folk medicine for treating ... more Plectranthus amboinicus (Lour.) Spreng. are generally used in Chinese folk
medicine for treating fever, cough, mumps, sore throats, and mosquito bite.
The current study was therefore carried out to provide vital pharmacological details about the leaf. This study investigated the anti-inϐlammatory properties of ethanolic extract from Plectranthus amboinicus. The leaves of Plectranthus amboinicus were rinsed, air dried and then powdered using machine.
The ethanol extract of the plant was used for phytochemical analysis to identify the constituents present in the plants. HPTLC analysis was carried out to
investigate its secondary metabolite proϐile by using the solvent mixture. The
anti-inϐlammatory effect was analysed by inhibition of protein denaturation
assay. The hypotonicity induced haemolysis was observed from 100µg/ml
to 500µg/ml of extract concentration. The results proved that the ethanolic leaf extract of P.amboinicus has antiinϐlammatory activity. The presence
of ϐlavonoid, quercetin in plant extract was conϐirmed by HPTLC analysis and
these ϐlavonoids may be responsible for the antiinϐlammatory activity. Further
studies are needed to the development of potential drug that may be used for
various pharmacological activities

Research paper thumbnail of HPTLC Analysis: Identification of Flavonoid from Senna alata (L.) Flowers

The aim of the study is to develop the high performance thin layer chromatography (HPTLC) fingerp... more The aim of the study is to develop the high performance thin layer chromatography (HPTLC) fingerprint profile of flavonoid of Senna alata (L.) HPTLC method for the separation of the active constituents in extracts has been developed using solvent system Toluene: Ethyl acetate: Formic acid (5:4:1). The preliminary phytochemical results of S.alata indicated that the presence of alkaloids, flavonoids, saponins, tannins and glycosides. In HPTLC analysis, it showed the presence of flavonoid quercetin in standard as well as the sample with Rf value 0.57. In future, these fingerprinting images will be helpful in the identification and quality control of the drug and ensure therapeutic efficacy. T.Ananthi* and R.Keerthana PG and Research Department of Biochemistry, S.T.E.T Women’s College, Sundarakkottai, Mannargudi, Thiruvarur Dt, Tamil Nadu, India Submission: 5 May 2016 Accepted: 10 May 2016 Published: 25 May 2016 www.ijsrm.humanjournals.com Citation: T.Ananthi et al. Ijsrm.Human, 2016; V...

Research paper thumbnail of Production of Pleurotus platypus and its nutrient analysis

Mushrooms have been a food supplement in various cu ltures and they are cultivated and eaten for ... more Mushrooms have been a food supplement in various cu ltures and they are cultivated and eaten for their edibility and delicacy. They fall between the best vegetables and animal protein source. Mushroom consider as a dist inctive food since the beginning of human evolution. As a fruit body of an edible white rotungus, oyster mushroom b elongs to Pleurotus, Pleurotaceae, Agaricales and Basidiomyco ta. One species of oyster mushroom Pleurotus platyp us spawn were produced by using sorghum grains. The mushroom was grown on paddy straw and average yield was calculated 410g and 273g in first and second harves ting. Whereas average yield 910g was recorded. The biochemical analysis confirms that the protein, car bohydrate, lipid and amino acid in Pleurotus platyp us. The total protein was 9.1 mg/g, carbohydrate 6.7mg/g, lipid 1 .27 mg/g and amino acid 1.35 mg/g were recorded.

Research paper thumbnail of Hepatoprotective Effect of Moringa oleifera in Isoniazid Induced Rats

The present study was carried out to screen and evaluate the hepatoprotective activity of flower ... more The present study was carried out to screen and evaluate the hepatoprotective activity of flower extract of Moringa oleifera. Hepatoprotective activity of ethyl acetate extract of Moringa oleifera was examined against Isoniazid induced liver damage in rats using silymarin as control. Enzyme activities of Aspartate Transaminase (AST), Alanine Transaminase (ALT), Catalase and Thio barbituric Acid Reactive substance (TBARS) were analyzed. Results indicate that ethyl acetate extract of M.oleifera has potent activity over isoniazid treatment as compared to control. Results of the present investigation confirm the traditional uses of this plant as a potential hepatoprotective agent.

Research paper thumbnail of HPTLC fingerprinting of stem bark extract of Nyctanthes arbor-tristis (L.)

Journal of Drug Delivery and Therapeutics, 2019

Fingerprint profile of bark extract of Nyctanthes arbor-tristis using High Performance Thin Lay... more Fingerprint profile of bark extract of Nyctanthes arbor-tristis using High Performance Thin Layer chromatography (HPTLC) has been established. HPTLC is a valuable tool for the investigation of medicinal plants with reference to the qualitative analysis of the phytoconstituents. Separation of the active constituents from the extracts has been developed using solvent system of Toluene: Ethyl acetate: Formic acid (5:4:1). The HPTLC analysis showed the presence of the flavonoid quercetin in the standard as well as in the sample and the Rf value was 0.73. These images of fingerprinting help in the proper identification and quantification of the marker compounds. On the basis of the marker compounds, new drugs could be formulated to treat various diseases Keywords: Nyctanthes arbor- tristis, HPTLC analysis, quercetin, bark extract

Research paper thumbnail of American Journal of Advanced Drug Delivery www.ajadd.co.uk American Journal of Advanced Drug Delivery www.ajadd.co.uk Original Article In vitro Evaluation of Antioxidant Activity

The aim of the present study was to investigate the antioxidant activity of methanolic extract of... more The aim of the present study was to investigate the antioxidant activity of methanolic extract of Michelia champaca flowers. Antioxidant activity was evaluated by using in-vitro assay models like Total antioxidant capacity, Nitric oxide radical scavenging assay, Reducing Power Assay and Hydrogen Peroxide Scavenging Activity. All the antioxidant activities were compared with standard antioxidant such as ascorbic acid. Methanolic extract of the plant showed effective free radical scavenging activity at 300µg concentration. The IC50 values of the ethanolic extract of Michelia champaca flowers were found to be 260 µg in total antioxidant activity, 150 µg in nitric oxide radical assay, 240 µg in reducing power assay and 280 µg in hydrogen peroxide assay. The results obtained in this study showed that the flowers of Michelia champaca have antioxidant properties which provide a basis for the traditional use of the plant.

Research paper thumbnail of Hepatoprotective activity of Michelia champaca L. against carbontetrachloride induced hepatic injury in rats

Michelia champaca (Magnoliaceae), commonly known as Svarna champa is a glorious traditional India... more Michelia champaca (Magnoliaceae), commonly known as Svarna champa is a glorious traditional Indian med icinal plant. The aim of this study was to evaluate the he patoprotective activity against CCl 4 induced liver injury in rats. Methanolic flower extract of Michelia champaca was investigated against CCl 4 induced hepatotoxicity and compared with standard drug silymarin. Liver marker enzymes (AST, ALT, ALP and GGT) and Renal markers (Urea, Creatinine and Total Bilirubin) were evaluat ed in control and experimental rats. CCl 4 treated rats elevated the liver marker enzymes and renal markers. However treatment with M.champaca significantly reversed t he above changes compared to the control group as observed i n the CCl 4 treated rats. The results clearly indicate that fl ower extract of Michelia champaca possess promising hepa toprotective effect.

Research paper thumbnail of GC-MS analysis of stem bark extracts of Senna alata (L.)

The aim of the study was to investigate the stem bark Extracts of Senna alata phytochemical compo... more The aim of the study was to investigate the stem bark Extracts of Senna alata phytochemical compounds of ethanol extract. The phyto compounds were screened by qualitative and GC-MS method. Qualitatively the different extracts were analyzed the compounds were found to like alkaloids, saponins flavonoids, tannins, glycosides, whereas reducing sugar, quinines, and coumarins, totally absent in this plant. In the GC-MS analysis, 22 bioactive compounds were identified in the ethanol extract of stem bark of Senna alata.

Research paper thumbnail of Effect of chromium on tannery workers in Tiruchirapalli District

Research paper thumbnail of Physicochemical And Phytochemical Studies On Jatropha Curcas

Jatropha curcas is a species of the genus Jatropha, belonging to the family euphorbiaceae. The st... more Jatropha curcas is a species of the genus Jatropha, belonging to the family euphorbiaceae. The study includes preparation of different extracts by successive solvent extraction for detailed analysis. Qualitative phytochemical analysis of these plant confirm the presence of various phytochemicals compounds in their ethanolic extracts. Different physicochemical parameters such as total ash value, percentage of solubility were analyzed. Fluorescence analysis of Jatropha curcas powder samples were noted under UV light and normal ordinary light, which signifies there characteristics. The different colored amino acid molecule with different of values constituted by paper chrmatography.

Research paper thumbnail of HPTLC analysis of Plumeria acaminata

Research paper thumbnail of Invitro clonal propagation of Tylophora indica

Research paper thumbnail of Isolation and characterization of extracellular cellulase using  Bacilluls subtilis from mangrove soil

A novel thermostable extracellular cellulases producing Bacillus subtilis was isolated from Mangr... more A novel thermostable extracellular cellulases producing Bacillus subtilis was isolated from Mangrove soil. The bacteria were grown on carboxymethyl cellulose agar at 45 o C and screened for the cellulase activity using Congo red method. The gram staining and biochemical tests had confirmed the microorganisms as Bacillus subtilis. Cellulose is commonly degraded by an enzyme called "cellulase". Cellulase is used for commercial food processing in coffee, textile industry and in laundry detergents. Maximum enzymatic activity was found at following optical parameters pH 7 (0.382 IU/ml) temperature 45 o C (0.620 IU/ml); nitrogen source 0.6g (0.398 IU/ml) and incubation period 5 days. In addition, protein determination of different pH, temperatures and nitrogen sources was found to be most suitable for cellulase production.

Research paper thumbnail of Saraswati 2008

Research paper thumbnail of Internationally indexed journal Internationally indexed journal Internationally indexed journal Internationally indexed journal

The "International Journal of Pharma and Bio Sciences" (IJPBS) is an international journal in Eng... more The "International Journal of Pharma and Bio Sciences" (IJPBS) is an international journal in English published quarterly. The aim of IJPBS is to publish peer reviewed research and review articles rapidly without delay in the developing field of pharmaceutical and biological sciences www.ijpbs.net Indexed in Elsevier Bibliographic Database

Research paper thumbnail of Production of Pleurotus platypus and its nutrient analysis

Mushrooms have been a food supplement in various cultures and they are cultivated and eaten for t... more Mushrooms have been a food supplement in various cultures and they are cultivated and eaten for their edibility and delicacy. They fall between the best vegetables and animal protein source. Mushroom consider as a distinctive food since the beginning of human evolution. As a fruit body of an edible white rotungus, oyster mushroom belongs to Pleurotus, Pleurotaceae, Agaricales and Basidiomycota. One species of oyster mushroom Pleurotus platypus spawn were produced by using sorghum grains. The mushroom was grown on paddy straw and average yield was calculated 410g and 273g in first and second harvesting. Whereas average yield 910g was recorded. The biochemical analysis confirms that the protein, carbohydrate, lipid and amino acid in Pleurotus platypus. The total protein was 9.1 mg/g, carbohydrate 6.7mg/g, lipid 1.27 mg/g and amino acid 1.35 mg/g were recorded.

Research paper thumbnail of Anti-inflammatory effect of Aegle marmelos in albino rats

Research paper thumbnail of EVALUATION OF PHYTOCHEMICAL SCREENING AND PHARMACOLOGICAL ACTIVITIES OF PORTULACA OLERACEA LEAVES

This study was carried out in aqueous extracts of the dried leaves of Portulaca oleracea and used... more This study was carried out in aqueous extracts of the dried leaves of Portulaca oleracea and used for phytochemicals, physiochemical antioxidant, antimicrobial and anti inflammatory analysis. By detecting zone inhibition, the antibacterial activity was determined against different bacterial and fungal strains. Phytochemical investigation of this plant determines alkaloids, phenols, flavonoid, tannins and saponin which is confirmed to be rich in leaves. The antioxidant activity was calculated by in vitro assay. The anti inflammatory activity of plant was done by protein denaturation method. The physiochemical result obtained can be used for the identification of powdered drugs. The antioxidant activity determination revealed that leaf extract had the highest antioxidant activity on DPPH free radicals. The results of the present study conclude that the studied plant possesses broadspectrum antibacterial and antioxidant properties and may act as a potent antioxidant for biological systems susceptible to free radical-mediated reactions.

Research paper thumbnail of HPTLC, FTIR AND GC-MS ANALYSIS OF ETHANOL EXTRACT OF Talinum triangulare (Jacq.) Willd

Annals of Roman Society and Cell Biology, 2021

Medicinal plants are the important bio resources of drugs for traditional system of medicine. The... more Medicinal plants are the important bio resources of drugs for traditional system of medicine. They comprise of various natural dynamic fixings; in this manner they are utilized for the treatment of an extensive number of irresistible sicknesses. The aim of the present study to identify the phytoconstituents from the Talinum triangulare leaves using various techniques such as FTIR, HPTLC and GC-MS analysis.

Research paper thumbnail of Physicochemical, phytochemicals and antioxidant activity of leaves of Sesbania grandiflora (L

The antioxidant activity of aqueous leaf extracts of Sesbania grandiflora was investigated using ... more The antioxidant activity of aqueous leaf extracts of Sesbania grandiflora was investigated using the DPPH radical scavenging assay, Hydrogen peroxide assay and Total antioxidant activity. In all testing a positive correlation existed between concentration of the extract and percentage inhibition of free radicals. Phytochemical screening, proximate analysis, mineral analysis by XRF were also determined. Qualitative screening for phytochemical showed the presence of alkaloids, flavonoids, phenols, tannins, terpenoids and cardiac glycosides. Proximate and mineral analysis showed moisture content (75.4%), ash content (2.24%), crude fibre (15.77%), crude lipid (1.81%), protein (7.14%), carbohydrate (12.8%) and minerals namely sodium, potassium and calcium which highlighted its good nutritional value. The results concluded that the presence of phytochemicals and some minerals proves that it is really an antioxidative.

Research paper thumbnail of HPTLC analysis and anti-inϐlammatory activity of Plectranthus amboinicus (Lour.) leaves

International Journal of Research in Pharmaceutical Sciences, 2021

Plectranthus amboinicus (Lour.) Spreng. are generally used in Chinese folk medicine for treating ... more Plectranthus amboinicus (Lour.) Spreng. are generally used in Chinese folk
medicine for treating fever, cough, mumps, sore throats, and mosquito bite.
The current study was therefore carried out to provide vital pharmacological details about the leaf. This study investigated the anti-inϐlammatory properties of ethanolic extract from Plectranthus amboinicus. The leaves of Plectranthus amboinicus were rinsed, air dried and then powdered using machine.
The ethanol extract of the plant was used for phytochemical analysis to identify the constituents present in the plants. HPTLC analysis was carried out to
investigate its secondary metabolite proϐile by using the solvent mixture. The
anti-inϐlammatory effect was analysed by inhibition of protein denaturation
assay. The hypotonicity induced haemolysis was observed from 100µg/ml
to 500µg/ml of extract concentration. The results proved that the ethanolic leaf extract of P.amboinicus has antiinϐlammatory activity. The presence
of ϐlavonoid, quercetin in plant extract was conϐirmed by HPTLC analysis and
these ϐlavonoids may be responsible for the antiinϐlammatory activity. Further
studies are needed to the development of potential drug that may be used for
various pharmacological activities

Research paper thumbnail of HPTLC Analysis: Identification of Flavonoid from Senna alata (L.) Flowers

The aim of the study is to develop the high performance thin layer chromatography (HPTLC) fingerp... more The aim of the study is to develop the high performance thin layer chromatography (HPTLC) fingerprint profile of flavonoid of Senna alata (L.) HPTLC method for the separation of the active constituents in extracts has been developed using solvent system Toluene: Ethyl acetate: Formic acid (5:4:1). The preliminary phytochemical results of S.alata indicated that the presence of alkaloids, flavonoids, saponins, tannins and glycosides. In HPTLC analysis, it showed the presence of flavonoid quercetin in standard as well as the sample with Rf value 0.57. In future, these fingerprinting images will be helpful in the identification and quality control of the drug and ensure therapeutic efficacy. T.Ananthi* and R.Keerthana PG and Research Department of Biochemistry, S.T.E.T Women’s College, Sundarakkottai, Mannargudi, Thiruvarur Dt, Tamil Nadu, India Submission: 5 May 2016 Accepted: 10 May 2016 Published: 25 May 2016 www.ijsrm.humanjournals.com Citation: T.Ananthi et al. Ijsrm.Human, 2016; V...

Research paper thumbnail of Production of Pleurotus platypus and its nutrient analysis

Mushrooms have been a food supplement in various cu ltures and they are cultivated and eaten for ... more Mushrooms have been a food supplement in various cu ltures and they are cultivated and eaten for their edibility and delicacy. They fall between the best vegetables and animal protein source. Mushroom consider as a dist inctive food since the beginning of human evolution. As a fruit body of an edible white rotungus, oyster mushroom b elongs to Pleurotus, Pleurotaceae, Agaricales and Basidiomyco ta. One species of oyster mushroom Pleurotus platyp us spawn were produced by using sorghum grains. The mushroom was grown on paddy straw and average yield was calculated 410g and 273g in first and second harves ting. Whereas average yield 910g was recorded. The biochemical analysis confirms that the protein, car bohydrate, lipid and amino acid in Pleurotus platyp us. The total protein was 9.1 mg/g, carbohydrate 6.7mg/g, lipid 1 .27 mg/g and amino acid 1.35 mg/g were recorded.

Research paper thumbnail of Hepatoprotective Effect of Moringa oleifera in Isoniazid Induced Rats

The present study was carried out to screen and evaluate the hepatoprotective activity of flower ... more The present study was carried out to screen and evaluate the hepatoprotective activity of flower extract of Moringa oleifera. Hepatoprotective activity of ethyl acetate extract of Moringa oleifera was examined against Isoniazid induced liver damage in rats using silymarin as control. Enzyme activities of Aspartate Transaminase (AST), Alanine Transaminase (ALT), Catalase and Thio barbituric Acid Reactive substance (TBARS) were analyzed. Results indicate that ethyl acetate extract of M.oleifera has potent activity over isoniazid treatment as compared to control. Results of the present investigation confirm the traditional uses of this plant as a potential hepatoprotective agent.

Research paper thumbnail of HPTLC fingerprinting of stem bark extract of Nyctanthes arbor-tristis (L.)

Journal of Drug Delivery and Therapeutics, 2019

Fingerprint profile of bark extract of Nyctanthes arbor-tristis using High Performance Thin Lay... more Fingerprint profile of bark extract of Nyctanthes arbor-tristis using High Performance Thin Layer chromatography (HPTLC) has been established. HPTLC is a valuable tool for the investigation of medicinal plants with reference to the qualitative analysis of the phytoconstituents. Separation of the active constituents from the extracts has been developed using solvent system of Toluene: Ethyl acetate: Formic acid (5:4:1). The HPTLC analysis showed the presence of the flavonoid quercetin in the standard as well as in the sample and the Rf value was 0.73. These images of fingerprinting help in the proper identification and quantification of the marker compounds. On the basis of the marker compounds, new drugs could be formulated to treat various diseases Keywords: Nyctanthes arbor- tristis, HPTLC analysis, quercetin, bark extract

Research paper thumbnail of American Journal of Advanced Drug Delivery www.ajadd.co.uk American Journal of Advanced Drug Delivery www.ajadd.co.uk Original Article In vitro Evaluation of Antioxidant Activity

The aim of the present study was to investigate the antioxidant activity of methanolic extract of... more The aim of the present study was to investigate the antioxidant activity of methanolic extract of Michelia champaca flowers. Antioxidant activity was evaluated by using in-vitro assay models like Total antioxidant capacity, Nitric oxide radical scavenging assay, Reducing Power Assay and Hydrogen Peroxide Scavenging Activity. All the antioxidant activities were compared with standard antioxidant such as ascorbic acid. Methanolic extract of the plant showed effective free radical scavenging activity at 300µg concentration. The IC50 values of the ethanolic extract of Michelia champaca flowers were found to be 260 µg in total antioxidant activity, 150 µg in nitric oxide radical assay, 240 µg in reducing power assay and 280 µg in hydrogen peroxide assay. The results obtained in this study showed that the flowers of Michelia champaca have antioxidant properties which provide a basis for the traditional use of the plant.

Research paper thumbnail of Hepatoprotective activity of Michelia champaca L. against carbontetrachloride induced hepatic injury in rats

Michelia champaca (Magnoliaceae), commonly known as Svarna champa is a glorious traditional India... more Michelia champaca (Magnoliaceae), commonly known as Svarna champa is a glorious traditional Indian med icinal plant. The aim of this study was to evaluate the he patoprotective activity against CCl 4 induced liver injury in rats. Methanolic flower extract of Michelia champaca was investigated against CCl 4 induced hepatotoxicity and compared with standard drug silymarin. Liver marker enzymes (AST, ALT, ALP and GGT) and Renal markers (Urea, Creatinine and Total Bilirubin) were evaluat ed in control and experimental rats. CCl 4 treated rats elevated the liver marker enzymes and renal markers. However treatment with M.champaca significantly reversed t he above changes compared to the control group as observed i n the CCl 4 treated rats. The results clearly indicate that fl ower extract of Michelia champaca possess promising hepa toprotective effect.

Research paper thumbnail of GC-MS analysis of stem bark extracts of Senna alata (L.)

The aim of the study was to investigate the stem bark Extracts of Senna alata phytochemical compo... more The aim of the study was to investigate the stem bark Extracts of Senna alata phytochemical compounds of ethanol extract. The phyto compounds were screened by qualitative and GC-MS method. Qualitatively the different extracts were analyzed the compounds were found to like alkaloids, saponins flavonoids, tannins, glycosides, whereas reducing sugar, quinines, and coumarins, totally absent in this plant. In the GC-MS analysis, 22 bioactive compounds were identified in the ethanol extract of stem bark of Senna alata.

Research paper thumbnail of Effect of chromium on tannery workers in Tiruchirapalli District

Research paper thumbnail of Physicochemical And Phytochemical Studies On Jatropha Curcas

Jatropha curcas is a species of the genus Jatropha, belonging to the family euphorbiaceae. The st... more Jatropha curcas is a species of the genus Jatropha, belonging to the family euphorbiaceae. The study includes preparation of different extracts by successive solvent extraction for detailed analysis. Qualitative phytochemical analysis of these plant confirm the presence of various phytochemicals compounds in their ethanolic extracts. Different physicochemical parameters such as total ash value, percentage of solubility were analyzed. Fluorescence analysis of Jatropha curcas powder samples were noted under UV light and normal ordinary light, which signifies there characteristics. The different colored amino acid molecule with different of values constituted by paper chrmatography.

Research paper thumbnail of HPTLC analysis of Plumeria acaminata

Research paper thumbnail of Invitro clonal propagation of Tylophora indica

Research paper thumbnail of Isolation and characterization of extracellular cellulase using  Bacilluls subtilis from mangrove soil

A novel thermostable extracellular cellulases producing Bacillus subtilis was isolated from Mangr... more A novel thermostable extracellular cellulases producing Bacillus subtilis was isolated from Mangrove soil. The bacteria were grown on carboxymethyl cellulose agar at 45 o C and screened for the cellulase activity using Congo red method. The gram staining and biochemical tests had confirmed the microorganisms as Bacillus subtilis. Cellulose is commonly degraded by an enzyme called "cellulase". Cellulase is used for commercial food processing in coffee, textile industry and in laundry detergents. Maximum enzymatic activity was found at following optical parameters pH 7 (0.382 IU/ml) temperature 45 o C (0.620 IU/ml); nitrogen source 0.6g (0.398 IU/ml) and incubation period 5 days. In addition, protein determination of different pH, temperatures and nitrogen sources was found to be most suitable for cellulase production.

Research paper thumbnail of Saraswati 2008

Research paper thumbnail of Internationally indexed journal Internationally indexed journal Internationally indexed journal Internationally indexed journal

The "International Journal of Pharma and Bio Sciences" (IJPBS) is an international journal in Eng... more The "International Journal of Pharma and Bio Sciences" (IJPBS) is an international journal in English published quarterly. The aim of IJPBS is to publish peer reviewed research and review articles rapidly without delay in the developing field of pharmaceutical and biological sciences www.ijpbs.net Indexed in Elsevier Bibliographic Database

Research paper thumbnail of Production of Pleurotus platypus and its nutrient analysis

Mushrooms have been a food supplement in various cultures and they are cultivated and eaten for t... more Mushrooms have been a food supplement in various cultures and they are cultivated and eaten for their edibility and delicacy. They fall between the best vegetables and animal protein source. Mushroom consider as a distinctive food since the beginning of human evolution. As a fruit body of an edible white rotungus, oyster mushroom belongs to Pleurotus, Pleurotaceae, Agaricales and Basidiomycota. One species of oyster mushroom Pleurotus platypus spawn were produced by using sorghum grains. The mushroom was grown on paddy straw and average yield was calculated 410g and 273g in first and second harvesting. Whereas average yield 910g was recorded. The biochemical analysis confirms that the protein, carbohydrate, lipid and amino acid in Pleurotus platypus. The total protein was 9.1 mg/g, carbohydrate 6.7mg/g, lipid 1.27 mg/g and amino acid 1.35 mg/g were recorded.

Research paper thumbnail of Anti-inflammatory effect of Aegle marmelos in albino rats

Research paper thumbnail of EVALUATION OF PHYTOCHEMICAL SCREENING AND PHARMACOLOGICAL ACTIVITIES OF PORTULACA OLERACEA LEAVES

This study was carried out in aqueous extracts of the dried leaves of Portulaca oleracea and used... more This study was carried out in aqueous extracts of the dried leaves of Portulaca oleracea and used for phytochemicals, physiochemical antioxidant, antimicrobial and anti inflammatory analysis. By detecting zone inhibition, the antibacterial activity was determined against different bacterial and fungal strains. Phytochemical investigation of this plant determines alkaloids, phenols, flavonoid, tannins and saponin which is confirmed to be rich in leaves. The antioxidant activity was calculated by in vitro assay. The anti inflammatory activity of plant was done by protein denaturation method. The physiochemical result obtained can be used for the identification of powdered drugs. The antioxidant activity determination revealed that leaf extract had the highest antioxidant activity on DPPH free radicals. The results of the present study conclude that the studied plant possesses broadspectrum antibacterial and antioxidant properties and may act as a potent antioxidant for biological systems susceptible to free radical-mediated reactions.

Research paper thumbnail of EVALUATION OF PRELIMINARY ANTIBACTERIAL ACTIVITY AND UV- SPECROSCOPIC ANALYSIS OF MICHELIA CHAMPACA (L

The aim of the present study was undertaken to appraise the UV-VIS analysis and antibacterial act... more The aim of the present study was undertaken to appraise the UV-VIS analysis and antibacterial activity of flowers
of Michelia champaca. The phytochemical analysis revealed the presence of carbohydrate, alkaloids, terpenoids,
flavonoids, tannins, steroids and phenols. The UV-VIS profile of flower extract showed the peaks at 281.1 nm and
215.6 nm with the absorption of 2.397 and 4.040. The antibacterial activity of flower extract against gram positive
(Staphylococcus aureus and Bacillus subtilis) and gram negative bacteria (E.coli) were tested by using disc
diffusion method. Ampicilin was used as standard. The results obtained shows that the extract expressed
remarkable antibacterial activity with zone of inhibition ranging from 9 to 19 mm. Maximum inhibition zone was
recorded with Bacillus subtilis (19.6 mm) while minimum inhibition zone was recorded for E.coli (9.37mm). The
antibacterial activity increased linearly with increase in concentration of the extract. The antibacterial activity of
Michelia champaca was due to the presence of various bioactive constituents present in the flower extract. The
results provide a support for the use of this plant in traditional medicine and it is a potential antiseptic source for
prevention and treatment of bacterial infections.