Junaid A M E E R Malik | Central University of Punjab (original) (raw)

Papers by Junaid A M E E R Malik

Nucb Journal of Language Culture and Communication, 2010

Indian Journal of Medical Specialities, 2013

A 17 year old male presented with one month history of difficulty in swallowing, reduced hearing ... more A 17 year old male presented with one month history of difficulty in swallowing, reduced hearing with intermittent pain in right ear, occasional nasal bleeding; slurring of speech and occasional headache with a few episodes of fainting. Clinical examination revealed right hypoglossal nerve palsy, and right lateral pharyngeal wall and tonsil were displaced medially. Right serous otitis media along with ipsilateral cervical and axillary lymphadenopathy were present. Blood biochemistry and urine examination were normal. An irregular heterogenous-enhancing mass in the right parapharyngeal space encasing the internal and external carotid arteries was evident on CT. Bone marrow biopsy was free from lymphomatous involvement. Intraoral biopsy from the nasopharyngeal bulge was consistent with a submucosal malignant round cell tumour. Immunohistochemistry showed tumour cells which were CD20 and CD22 positive, and negative for CD5, Desmin, Cytokeratin (CK), CD99, and synaptophysin; with a proliferative fraction (Ki-67 index) of greater than 98%. A diagnosis of Burkitt's lymphoma of the parapharyngeal space, stage II E was made. Patient responded to chemotherapy. Sporadic Burkitt's lymphoma in an immunocompetent Indian male is being reported because of its unusual location-parapharyngeal space.

Eastern Mediterranean health journal = La revue de sante de la Mediterranee orientale = al-Majallah al-sihhiyah li-sharq al-mutawassit, 2007

We studied the prevalence and factors associated with overweight and obesity among 200 non-pregna... more We studied the prevalence and factors associated with overweight and obesity among 200 non-pregnant Iraqi women (> 18 years) attending 2 outpatient clinics in Baghdad. The women were interviewed using a questionnaire and had their body mass index (BMI) and waist-to-hip ratio (WHR) calculated. Only 24% of the women were normal weight: 39%, 25% and 12% were overweight (BMI 25-29.9 kg/m2), obese (> or =30 kg/m2) and morbidly obese (> or = 40 kg/m2) respectively. While the mean WHR was normal, the mean waist circumference was higher than normal. Factors significantly associated with overweight and obesity were older age, history of previous laparotomy and type of clothing worn (gown) inside the home.

Genes, 2021

The outbreak of coronavirus disease 2019 (COVID-19), by the severe acute respiratory syndrome cor... more The outbreak of coronavirus disease 2019 (COVID-19), by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has quickly developed into a worldwide pandemic. Mutations in the SARS-CoV-2 genome may affect various aspects of the disease including fatality ratio. In this study, 553,518 SARS-CoV-2 genome sequences isolated from patients from continents for the period 1 December 2020 to 15 March 2021 were comprehensively analyzed and a total of 82 mutations were identified concerning the reference sequence. In addition, associations between the mutations and the case fatality ratio (CFR), cases per million and deaths per million, were examined. The mutations having the highest frequencies among different continents were Spike_D614G and NSP12_P323L. Among the identified mutations, NSP2_T153M, NSP14_I42V and Spike_L18F mutations showed a positive correlation to CFR. While the NSP13_Y541C, NSP3_T73I and NSP3_Q180H mutations demonstrated a negative correlation to CFR. The Spike_...

Engineering and Technology Journal, 2019

استخدام النانوهيدروكسي اباتايت في اساس بوليمري كمادة نشطة بايولوجيةلهندسة انسجة العظم له اماكنية ... more استخدام النانوهيدروكسي اباتايت في اساس بوليمري كمادة نشطة بايولوجيةلهندسة انسجة العظم له اماكنية علاجية هائلة بسبب الخواص المفيدةمثل التوافقيةالبايولوجية، التحلل البايولوجي، ويعتبر مكونة غير عضوي رئيسية لااساس العظم. الاغشية المخلوطة من الشيتوزان والبولي فينيل الكحول قد تمت دراستها مع وبدون الجينبين والنانوهيدركسي اباتايت. العينات قد حضرت بطريقة صب المذيبات. وقداجري لها فحص تحويل فورييه بالاشعة تحت الحمرتحت الجمراء،سلوك التحلل، درجة الانتفاخو مقاومة الشد. النتائج اظهرت بأن متراكبة الشيتوزان ممكن استخدامها كمادة بايولوجية فعالة لهندسة تجدد العظم مع معدلات تحلل مختلفة.

BACKGROUND: Understanding the awareness and perception to infectious disease threats particularly... more BACKGROUND: Understanding the awareness and perception to infectious disease threats particularly among medical students is important to assist improving educational programs. OBJECTIVE: This study aimed to assess students’ knowledge of the pandemic H1N1 influenza. PATIENTS AND METHODS: A cross-sectional study was conducted among medical students of Al-Nahrain Medical College in Baghdad during February 2010. The data were collected through a self-administered anonymous questionnaire and results were analyzed using SPSS version 16. RESULTS: A total of 217 medical students participated in this study with a mean age of 21.1 (± 1.7). Overall, the mean score of students' knowledge was 69.95 (± 17.7) degrees. The main source of knowledge of these students (37.8%) was from the mass media. Significantly females, fourth grade, passed the last semester exams, and having educational activity dealt with swine flu scored higher. CONCLUSION: The awareness regarding pandemic H1N1 infection ...

Background and Objectives: To determine the most chromosomal abnormalities seen in Childhood Acut... more Background and Objectives: To determine the most chromosomal abnormalities seen in Childhood Acute Lymphoblastic Leukemia (CALL) in Mosul, and to evaluate the correlations between clinical haematological and chromosomal abnormalities in CALL. Pateints and Methods: Clinical notes, haematological parameters and cytogenetic analysis were studied for all patients. Cases were collected from oncology unit at Ibn Al-Atheer Teaching Hospital (ATH) in Mosul. Results: The frequency of normal karyotype was (42.9%) while the frequency of pseudodiploidy, hyperdiploidy and hypodiploidy were (8.5%), (28.6%) and (20%), respectively. Cases with hyperdiploidy had significantly low Total Leukocyte Count (TLC), higher platelet count with (P<0.001), (P<0.05), respectively. Massive Hepato-Splenomegaly (MHS) was seen mainly in hypodiploidy group (P<0.01).

International Journal of Biometeorology

Newborn length has been reported by many researchers to be reduced at high altitudes. However, ma... more Newborn length has been reported by many researchers to be reduced at high altitudes. However, many of these studies lacked adequate control of the ethnic group which may be confounding the altitude differences. In addition, few studies have examined the sources of variation in birth weight at high altitudes that may be related to ethnic group adaptation to the stresses of this hypoxic environment. In our study, we tested the hypotheses that the effect of altitude differences in newborn length depends on ethnic variation. Samples of 3359 healthy male newborns from different areas in Kyrgyzstan between the years 2003 and 2011 were analyzed for altitude and ethnic variation on male newborn length. Our results indicate significant decrease in male newborn length as a latitude increase. It is concluded that ethnic group difference in pregnancy outcome reflects a better state of adaptation to high altitude in this healthy indigenous population and that long-term genetic selection may be the most plausible explanation for these ethnic differences.

PLOS ONE

CD36 (cluster of differentiation 36) is a membrane protein involved in lipid metabolism and has b... more CD36 (cluster of differentiation 36) is a membrane protein involved in lipid metabolism and has been linked to pathological conditions associated with metabolic disorders, such as diabetes and dyslipidemia. A case-control study was conducted and included 177 patients with type-2 diabetes mellitus (T2DM) and 173 control subjects to study the involvement of CD36 gene rs1761667 (G>A) and rs1527483 (C>T) polymorphisms in the pathogenesis of T2DM and dyslipidemia among Jordanian population. Lipid profile, blood sugar, gender and age were measured and recorded. Also, genotyping analysis for both polymorphisms was performed. Following statistical analysis, 10 different neural networks and machine learning (ML) tools were used to predict subjects with diabetes or dyslipidemia. Towards further understanding of the role of CD36 protein and gene in T2DM and dyslipidemia, a protein-protein interaction network and meta-analysis were carried out. For both polymorphisms, the genotypic freque...

SSRN Electronic Journal

The coronavirus disease (COVID-19) is a highly pathogenic viral infection caused by Severe acute ... more The coronavirus disease (COVID-19) is a highly pathogenic viral infection caused by Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). World Health Organization (WHO) announced the COVID-19 outbreak as a pandemic on 11 March 2020. The B cells in our immune system play a major role in protecting against viruses, bacterium, or other pathogens by producing different classes of antibodies. This study aimed to evaluate the correlation between antibodies production (IgG and IgM) and several factors; age, sex, co-morbidity diseases, patient’s blood group, and Chest computed tomography (CT) findings. IgG and IgM antibody levels were determined for 500 COVID-19 Russian patients. There was a significant inverse correlation (χ2 = 92.53, df = 2, P < 0.001) between IgG antibody production and disease severity determined by CT scan. However, other clinical factors, such as blood group, and co-morbidity diseases, were not significantly associated with antibody production among COVID-19 patients. from the results in this study show that lung damage finding in CT decreases the immune system’s capacity and worsens symptoms and severity of COVID-19. Funding Statement: This paper has been supported by the RUDN University Strategic Academic Leadership Program. Declaration of Interests: The authors declare no conflict of interest. Ethics Approval Statement: The Ethical Committee approved the study of Clinical studies at the Peoples' Friendship University of Russia. (IRB no. 2019-0300/1198).

Endocrine, Metabolic & Immune Disorders - Drug Targets

Background: Fibromyalgia syndrome (FMS) is a chronic disease characterized by widespread body pai... more Background: Fibromyalgia syndrome (FMS) is a chronic disease characterized by widespread body pain, weakness in certain parts of the body (critical points), low pain tolerance, sleep disturbances, and fatigue. This syndrome is considered rare in Jordan. Objectives: The research aimed to find out the association of the angiotensin converting enzyme, methylenetetrahydrofolate reductase, and vitamin D receptor (ACE, MHFTR, and VDR, respectively) genotypes with FMS among Jordanian patients. Methods: This work included 22 FM patients and 22 healthy individuals of Jordanian Arabic origin. The ACE rs4646994, MTHFR rs1801133, and VDR rs2228570 genotypes were determined using polymerase chain reaction (PCR) followed by restriction fragment length polymorphism. Results: No associations between ACE rs4646994, MTHFR rs1801133, and VDR rs2228570 with the vulnerability of a person for the development of FMS were found. However, we found an association between the ACE rs4646994 genotype and restle...

Biology

Type 2 diabetes mellitus (T2DM) is a multifactorial disease associated with many genetic polymorp... more Type 2 diabetes mellitus (T2DM) is a multifactorial disease associated with many genetic polymorphisms; among them is the FokI polymorphism in the vitamin D receptor (VDR) gene. In this case-control study, samples from 82 T2DM patients and 82 healthy controls were examined to investigate the association of the FokI polymorphism and lipid profile with T2DM in the Jordanian population. DNA was extracted from blood and genotyped for the FokI polymorphism by polymerase chain reaction (PCR) and DNA sequencing. Lipid profile and fasting blood sugar were also measured. There were significant differences in high-density lipoprotein (HDL) cholesterol and triglyceride levels between T2DM and control samples. Frequencies of the FokI polymorphism (CC, CT and TT) were determined in T2DM and control samples and were not significantly different. Furthermore, there was no significant association between the FokI polymorphism and T2DM or lipid profile. A feed-forward neural network (FNN) was used as...

Current Pharmaceutical Design

Background: Oxandrolone is a synthetic testosterone analogue that is widely used among bodybuilde... more Background: Oxandrolone is a synthetic testosterone analogue that is widely used among bodybuilders and athletes. However, oxandrolone causes male infertility. Recently, it was found that metformin reduces the risk of infertility associated with diabetes mellitus. Aim: This study aimed to investigate the protective effects of metformin against oxandrolone-induced infertility in male rats. Methods: Rats continuously received one of four treatments (n=7) over 14 days: control DMSO administration, oxandrolone administration, metformin administration, or co-administration of oxandrolone and metformin. Doses were equivalent to those used for human treatment. Subsequently, testicular and blood samples were collected for morphological, biochemical, and histological examination. In addition, gene expression of the testosterone synthesizing enzyme CYP11A1 was analyzed in the testes using RT-PCR. Results: Oxandrolone administration induced male infertility by significantly reducing relative w...

Endocrine, Metabolic & Immune Disorders - Drug Targets

Background: Practitioners of traditional medicine use the decoction of Ononis natrix L. to treat ... more Background: Practitioners of traditional medicine use the decoction of Ononis natrix L. to treat hyperglycemia. The literature offers no evidence to support the use. Objective: To investigate the effect of the decoction of Ononis natrix L. on the blood glucose concentration in Wistar rats (Rattus norvegicus) with streptozotocin-induced diabetes mellitus. Methods: We obtained 35 Wistar rats from the animal colony of The University of Jordan School of Medicine. We induced diabetes by a single intraperitoneal injection of streptozotocin (60 mg/kg body weight) and 23 rats (66%) survived to allocation. We randomly assigned the rats to one of four groups: negative control (1% Tween 80 in distilled water), positive control (100 mg/kg metformin), high-dose treatment (7.5 mL of the decoction), and low-dose treatment (3.5 mL of the decoc-tion). We administered the doses twice daily by oral gavage for two weeks and measured the tail-blood glucose concentration twice daily, once before the firs...

Asian Pacific Journal of Cancer Prevention, Mar 1, 2020

1999). X-ray repair cross-complementing group 1 (XRCC1) is a key protein in BER and closely assoc... more 1999). X-ray repair cross-complementing group 1 (XRCC1) is a key protein in BER and closely associated with BER pathway coordination due to interacting with most components of the BER short-patch pathway (Caldecott et al., 1996; Marintchev et al., 2000). There are many single

npj Microgravity

Microgravity affects plant growth and content. A three-dimensional clinostat was used at 4 rotati... more Microgravity affects plant growth and content. A three-dimensional clinostat was used at 4 rotations/min to rotate the seeds of Triticum aestivum cultivar (Ammon) in three dimensions for 7 days, following which the antioxidant activities of ethanolic extracts were evaluated using both nitric oxide- and hydrogen peroxide-scavenging activities. The antidiabetic activities of ethanolic extracts were evaluated by measuring the concentration of plasma glucose, insulin, C peptide, and glycated hemoglobin (HbA1c); determining the number of β cells in the pancreatic islets; and performing the glucose tolerance test. Furthermore, the effects of the ethanolic extracts on the lipid profile and liver function were estimated. After rats were sacrificed, their pancreases were isolated and used for histopathological processing. The results indicated that the antioxidant potential and antioxidant metabolite content were significantly increased under microgravity conditions in comparison to those un...

IOP Conference Series: Materials Science and Engineering

In this paper, the biological and physical properties of chitosan, a potential polysaccharide res... more In this paper, the biological and physical properties of chitosan, a potential polysaccharide resource, were investigated. The proposed film was synthesised by mixing chitosan (CS) solution and polyvinyl alcohol (PVA) using solvent casting. The modified chitosan/PVA films were then subjected to analysis by Fourier transform infrared spectroscopy (FTIR), in which intermolecular hydrogen bonding between the molecules of CS and PVA was clearly demonstrated. The swelling degree of chitosan along with the contact angle were also examined. After the addition of genipin, which increased chitosan concentration the swelling degree, degradation rate, and contact angle was decreased. The results confirm that the properties of chitosan are such that they can be employed for tissue engineering applications.

Nucb Journal of Language Culture and Communication, 2010

Indian Journal of Medical Specialities, 2013

A 17 year old male presented with one month history of difficulty in swallowing, reduced hearing ... more A 17 year old male presented with one month history of difficulty in swallowing, reduced hearing with intermittent pain in right ear, occasional nasal bleeding; slurring of speech and occasional headache with a few episodes of fainting. Clinical examination revealed right hypoglossal nerve palsy, and right lateral pharyngeal wall and tonsil were displaced medially. Right serous otitis media along with ipsilateral cervical and axillary lymphadenopathy were present. Blood biochemistry and urine examination were normal. An irregular heterogenous-enhancing mass in the right parapharyngeal space encasing the internal and external carotid arteries was evident on CT. Bone marrow biopsy was free from lymphomatous involvement. Intraoral biopsy from the nasopharyngeal bulge was consistent with a submucosal malignant round cell tumour. Immunohistochemistry showed tumour cells which were CD20 and CD22 positive, and negative for CD5, Desmin, Cytokeratin (CK), CD99, and synaptophysin; with a proliferative fraction (Ki-67 index) of greater than 98%. A diagnosis of Burkitt's lymphoma of the parapharyngeal space, stage II E was made. Patient responded to chemotherapy. Sporadic Burkitt's lymphoma in an immunocompetent Indian male is being reported because of its unusual location-parapharyngeal space.

Eastern Mediterranean health journal = La revue de sante de la Mediterranee orientale = al-Majallah al-sihhiyah li-sharq al-mutawassit, 2007

We studied the prevalence and factors associated with overweight and obesity among 200 non-pregna... more We studied the prevalence and factors associated with overweight and obesity among 200 non-pregnant Iraqi women (> 18 years) attending 2 outpatient clinics in Baghdad. The women were interviewed using a questionnaire and had their body mass index (BMI) and waist-to-hip ratio (WHR) calculated. Only 24% of the women were normal weight: 39%, 25% and 12% were overweight (BMI 25-29.9 kg/m2), obese (> or =30 kg/m2) and morbidly obese (> or = 40 kg/m2) respectively. While the mean WHR was normal, the mean waist circumference was higher than normal. Factors significantly associated with overweight and obesity were older age, history of previous laparotomy and type of clothing worn (gown) inside the home.

Genes, 2021

The outbreak of coronavirus disease 2019 (COVID-19), by the severe acute respiratory syndrome cor... more The outbreak of coronavirus disease 2019 (COVID-19), by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has quickly developed into a worldwide pandemic. Mutations in the SARS-CoV-2 genome may affect various aspects of the disease including fatality ratio. In this study, 553,518 SARS-CoV-2 genome sequences isolated from patients from continents for the period 1 December 2020 to 15 March 2021 were comprehensively analyzed and a total of 82 mutations were identified concerning the reference sequence. In addition, associations between the mutations and the case fatality ratio (CFR), cases per million and deaths per million, were examined. The mutations having the highest frequencies among different continents were Spike_D614G and NSP12_P323L. Among the identified mutations, NSP2_T153M, NSP14_I42V and Spike_L18F mutations showed a positive correlation to CFR. While the NSP13_Y541C, NSP3_T73I and NSP3_Q180H mutations demonstrated a negative correlation to CFR. The Spike_...

Engineering and Technology Journal, 2019

استخدام النانوهيدروكسي اباتايت في اساس بوليمري كمادة نشطة بايولوجيةلهندسة انسجة العظم له اماكنية ... more استخدام النانوهيدروكسي اباتايت في اساس بوليمري كمادة نشطة بايولوجيةلهندسة انسجة العظم له اماكنية علاجية هائلة بسبب الخواص المفيدةمثل التوافقيةالبايولوجية، التحلل البايولوجي، ويعتبر مكونة غير عضوي رئيسية لااساس العظم. الاغشية المخلوطة من الشيتوزان والبولي فينيل الكحول قد تمت دراستها مع وبدون الجينبين والنانوهيدركسي اباتايت. العينات قد حضرت بطريقة صب المذيبات. وقداجري لها فحص تحويل فورييه بالاشعة تحت الحمرتحت الجمراء،سلوك التحلل، درجة الانتفاخو مقاومة الشد. النتائج اظهرت بأن متراكبة الشيتوزان ممكن استخدامها كمادة بايولوجية فعالة لهندسة تجدد العظم مع معدلات تحلل مختلفة.

BACKGROUND: Understanding the awareness and perception to infectious disease threats particularly... more BACKGROUND: Understanding the awareness and perception to infectious disease threats particularly among medical students is important to assist improving educational programs. OBJECTIVE: This study aimed to assess students’ knowledge of the pandemic H1N1 influenza. PATIENTS AND METHODS: A cross-sectional study was conducted among medical students of Al-Nahrain Medical College in Baghdad during February 2010. The data were collected through a self-administered anonymous questionnaire and results were analyzed using SPSS version 16. RESULTS: A total of 217 medical students participated in this study with a mean age of 21.1 (± 1.7). Overall, the mean score of students' knowledge was 69.95 (± 17.7) degrees. The main source of knowledge of these students (37.8%) was from the mass media. Significantly females, fourth grade, passed the last semester exams, and having educational activity dealt with swine flu scored higher. CONCLUSION: The awareness regarding pandemic H1N1 infection ...

Background and Objectives: To determine the most chromosomal abnormalities seen in Childhood Acut... more Background and Objectives: To determine the most chromosomal abnormalities seen in Childhood Acute Lymphoblastic Leukemia (CALL) in Mosul, and to evaluate the correlations between clinical haematological and chromosomal abnormalities in CALL. Pateints and Methods: Clinical notes, haematological parameters and cytogenetic analysis were studied for all patients. Cases were collected from oncology unit at Ibn Al-Atheer Teaching Hospital (ATH) in Mosul. Results: The frequency of normal karyotype was (42.9%) while the frequency of pseudodiploidy, hyperdiploidy and hypodiploidy were (8.5%), (28.6%) and (20%), respectively. Cases with hyperdiploidy had significantly low Total Leukocyte Count (TLC), higher platelet count with (P<0.001), (P<0.05), respectively. Massive Hepato-Splenomegaly (MHS) was seen mainly in hypodiploidy group (P<0.01).

International Journal of Biometeorology

Newborn length has been reported by many researchers to be reduced at high altitudes. However, ma... more Newborn length has been reported by many researchers to be reduced at high altitudes. However, many of these studies lacked adequate control of the ethnic group which may be confounding the altitude differences. In addition, few studies have examined the sources of variation in birth weight at high altitudes that may be related to ethnic group adaptation to the stresses of this hypoxic environment. In our study, we tested the hypotheses that the effect of altitude differences in newborn length depends on ethnic variation. Samples of 3359 healthy male newborns from different areas in Kyrgyzstan between the years 2003 and 2011 were analyzed for altitude and ethnic variation on male newborn length. Our results indicate significant decrease in male newborn length as a latitude increase. It is concluded that ethnic group difference in pregnancy outcome reflects a better state of adaptation to high altitude in this healthy indigenous population and that long-term genetic selection may be the most plausible explanation for these ethnic differences.

PLOS ONE

CD36 (cluster of differentiation 36) is a membrane protein involved in lipid metabolism and has b... more CD36 (cluster of differentiation 36) is a membrane protein involved in lipid metabolism and has been linked to pathological conditions associated with metabolic disorders, such as diabetes and dyslipidemia. A case-control study was conducted and included 177 patients with type-2 diabetes mellitus (T2DM) and 173 control subjects to study the involvement of CD36 gene rs1761667 (G>A) and rs1527483 (C>T) polymorphisms in the pathogenesis of T2DM and dyslipidemia among Jordanian population. Lipid profile, blood sugar, gender and age were measured and recorded. Also, genotyping analysis for both polymorphisms was performed. Following statistical analysis, 10 different neural networks and machine learning (ML) tools were used to predict subjects with diabetes or dyslipidemia. Towards further understanding of the role of CD36 protein and gene in T2DM and dyslipidemia, a protein-protein interaction network and meta-analysis were carried out. For both polymorphisms, the genotypic freque...

SSRN Electronic Journal

The coronavirus disease (COVID-19) is a highly pathogenic viral infection caused by Severe acute ... more The coronavirus disease (COVID-19) is a highly pathogenic viral infection caused by Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). World Health Organization (WHO) announced the COVID-19 outbreak as a pandemic on 11 March 2020. The B cells in our immune system play a major role in protecting against viruses, bacterium, or other pathogens by producing different classes of antibodies. This study aimed to evaluate the correlation between antibodies production (IgG and IgM) and several factors; age, sex, co-morbidity diseases, patient’s blood group, and Chest computed tomography (CT) findings. IgG and IgM antibody levels were determined for 500 COVID-19 Russian patients. There was a significant inverse correlation (χ2 = 92.53, df = 2, P < 0.001) between IgG antibody production and disease severity determined by CT scan. However, other clinical factors, such as blood group, and co-morbidity diseases, were not significantly associated with antibody production among COVID-19 patients. from the results in this study show that lung damage finding in CT decreases the immune system’s capacity and worsens symptoms and severity of COVID-19. Funding Statement: This paper has been supported by the RUDN University Strategic Academic Leadership Program. Declaration of Interests: The authors declare no conflict of interest. Ethics Approval Statement: The Ethical Committee approved the study of Clinical studies at the Peoples' Friendship University of Russia. (IRB no. 2019-0300/1198).

Endocrine, Metabolic & Immune Disorders - Drug Targets

Background: Fibromyalgia syndrome (FMS) is a chronic disease characterized by widespread body pai... more Background: Fibromyalgia syndrome (FMS) is a chronic disease characterized by widespread body pain, weakness in certain parts of the body (critical points), low pain tolerance, sleep disturbances, and fatigue. This syndrome is considered rare in Jordan. Objectives: The research aimed to find out the association of the angiotensin converting enzyme, methylenetetrahydrofolate reductase, and vitamin D receptor (ACE, MHFTR, and VDR, respectively) genotypes with FMS among Jordanian patients. Methods: This work included 22 FM patients and 22 healthy individuals of Jordanian Arabic origin. The ACE rs4646994, MTHFR rs1801133, and VDR rs2228570 genotypes were determined using polymerase chain reaction (PCR) followed by restriction fragment length polymorphism. Results: No associations between ACE rs4646994, MTHFR rs1801133, and VDR rs2228570 with the vulnerability of a person for the development of FMS were found. However, we found an association between the ACE rs4646994 genotype and restle...

Biology

Type 2 diabetes mellitus (T2DM) is a multifactorial disease associated with many genetic polymorp... more Type 2 diabetes mellitus (T2DM) is a multifactorial disease associated with many genetic polymorphisms; among them is the FokI polymorphism in the vitamin D receptor (VDR) gene. In this case-control study, samples from 82 T2DM patients and 82 healthy controls were examined to investigate the association of the FokI polymorphism and lipid profile with T2DM in the Jordanian population. DNA was extracted from blood and genotyped for the FokI polymorphism by polymerase chain reaction (PCR) and DNA sequencing. Lipid profile and fasting blood sugar were also measured. There were significant differences in high-density lipoprotein (HDL) cholesterol and triglyceride levels between T2DM and control samples. Frequencies of the FokI polymorphism (CC, CT and TT) were determined in T2DM and control samples and were not significantly different. Furthermore, there was no significant association between the FokI polymorphism and T2DM or lipid profile. A feed-forward neural network (FNN) was used as...

Current Pharmaceutical Design

Background: Oxandrolone is a synthetic testosterone analogue that is widely used among bodybuilde... more Background: Oxandrolone is a synthetic testosterone analogue that is widely used among bodybuilders and athletes. However, oxandrolone causes male infertility. Recently, it was found that metformin reduces the risk of infertility associated with diabetes mellitus. Aim: This study aimed to investigate the protective effects of metformin against oxandrolone-induced infertility in male rats. Methods: Rats continuously received one of four treatments (n=7) over 14 days: control DMSO administration, oxandrolone administration, metformin administration, or co-administration of oxandrolone and metformin. Doses were equivalent to those used for human treatment. Subsequently, testicular and blood samples were collected for morphological, biochemical, and histological examination. In addition, gene expression of the testosterone synthesizing enzyme CYP11A1 was analyzed in the testes using RT-PCR. Results: Oxandrolone administration induced male infertility by significantly reducing relative w...

Endocrine, Metabolic & Immune Disorders - Drug Targets

Background: Practitioners of traditional medicine use the decoction of Ononis natrix L. to treat ... more Background: Practitioners of traditional medicine use the decoction of Ononis natrix L. to treat hyperglycemia. The literature offers no evidence to support the use. Objective: To investigate the effect of the decoction of Ononis natrix L. on the blood glucose concentration in Wistar rats (Rattus norvegicus) with streptozotocin-induced diabetes mellitus. Methods: We obtained 35 Wistar rats from the animal colony of The University of Jordan School of Medicine. We induced diabetes by a single intraperitoneal injection of streptozotocin (60 mg/kg body weight) and 23 rats (66%) survived to allocation. We randomly assigned the rats to one of four groups: negative control (1% Tween 80 in distilled water), positive control (100 mg/kg metformin), high-dose treatment (7.5 mL of the decoction), and low-dose treatment (3.5 mL of the decoc-tion). We administered the doses twice daily by oral gavage for two weeks and measured the tail-blood glucose concentration twice daily, once before the firs...

Asian Pacific Journal of Cancer Prevention, Mar 1, 2020

1999). X-ray repair cross-complementing group 1 (XRCC1) is a key protein in BER and closely assoc... more 1999). X-ray repair cross-complementing group 1 (XRCC1) is a key protein in BER and closely associated with BER pathway coordination due to interacting with most components of the BER short-patch pathway (Caldecott et al., 1996; Marintchev et al., 2000). There are many single

npj Microgravity

Microgravity affects plant growth and content. A three-dimensional clinostat was used at 4 rotati... more Microgravity affects plant growth and content. A three-dimensional clinostat was used at 4 rotations/min to rotate the seeds of Triticum aestivum cultivar (Ammon) in three dimensions for 7 days, following which the antioxidant activities of ethanolic extracts were evaluated using both nitric oxide- and hydrogen peroxide-scavenging activities. The antidiabetic activities of ethanolic extracts were evaluated by measuring the concentration of plasma glucose, insulin, C peptide, and glycated hemoglobin (HbA1c); determining the number of β cells in the pancreatic islets; and performing the glucose tolerance test. Furthermore, the effects of the ethanolic extracts on the lipid profile and liver function were estimated. After rats were sacrificed, their pancreases were isolated and used for histopathological processing. The results indicated that the antioxidant potential and antioxidant metabolite content were significantly increased under microgravity conditions in comparison to those un...

IOP Conference Series: Materials Science and Engineering

In this paper, the biological and physical properties of chitosan, a potential polysaccharide res... more In this paper, the biological and physical properties of chitosan, a potential polysaccharide resource, were investigated. The proposed film was synthesised by mixing chitosan (CS) solution and polyvinyl alcohol (PVA) using solvent casting. The modified chitosan/PVA films were then subjected to analysis by Fourier transform infrared spectroscopy (FTIR), in which intermolecular hydrogen bonding between the molecules of CS and PVA was clearly demonstrated. The swelling degree of chitosan along with the contact angle were also examined. After the addition of genipin, which increased chitosan concentration the swelling degree, degradation rate, and contact angle was decreased. The results confirm that the properties of chitosan are such that they can be employed for tissue engineering applications.