Structural basis for the assembly of a nuclear export complex (original) (raw)

References

  1. Weis, K. Regulating access to the genome. Nucleocytoplasmic transport throughout the cell cycle. Cell 112, 441–451 (2003)
    Article CAS Google Scholar
  2. Mosammaparast, M. & Pemberton, L. Karyopherins: from nuclear transport mediators to nuclear function regulators. Trends Cell Biol. 14, 547–556 (2004)
    Article CAS Google Scholar
  3. Kobe, B. Autoinhibition by an internal nuclear localization signal revealed by the crystal structure of mammalian importin α. Nature Struct. Biol. 6, 388–397 (1999)
    Article CAS Google Scholar
  4. Hood, J. K. & Silver, P. A. Cse1p is required for export of Srp1p/importin-α from the nucleus in Saccharomyces cerevisiae. J. Biol. Chem. 273, 35142–35146 (1998)
    Article CAS Google Scholar
  5. Solsbacher, J., Maurer, P., Bischoff, F. R. & Schlenstedt, G. Cse1p is involved in export of yeast importin α from the nucleus. Mol. Cell. Biol. 18, 6805–6815 (1998)
    Article CAS Google Scholar
  6. Kutay, U., Bischoff, F. R., Kostka, S., Kraft, R. & Gorlich, D. Export of importin α from the nucleus is mediated by a specific nuclear transport factor. Cell 90, 1061–1071 (1997)
    Article CAS Google Scholar
  7. Cingolani, G., Petose, C., Weis, K. & Muller, C. Structure of importin-β bound to the IBB domain of importin-α. Nature 399, 221–229 (1999)
    Article ADS CAS Google Scholar
  8. Chook, Y. M. & Blobel, G. Structure of the nuclear transport complex karyopherin-β2-Ran·GppNHp. Nature 399, 230–237 (1999)
    Article ADS CAS Google Scholar
  9. Vetter, I. R., Arndt, A., Kutay, U., Gorlich, D. & Wittinghofer, A. Structural view of the Ran-Importin β interaction at 2.3 Å resolution. Cell 97, 635–646 (1999)
    Article CAS Google Scholar
  10. Lee, S. J. et al. The structure of importin-β bound to SREBP-2: nuclear import of a transcription factor. Science 302, 1571–1575 (2003)
    Article ADS CAS Google Scholar
  11. Bayliss, R., Littlewood, T. & Stewart, M. Structural basis for the interaction between FxFG nucleoporin repeats and importin-β in nuclear trafficking. Cell 102, 99–108 (2000)
    Article CAS Google Scholar
  12. Nilsson, J., Weis, K. & Kjems, J. The C-terminal extension of the small GTPase Ran is essential for defining the GDP-bound form. J. Mol. Biol. 318, 583–593 (2002)
    Article CAS Google Scholar
  13. Herold, A., Truant, R., Wiegand, H. & Cullen, B. R. Determination of the functional organization of the importin α nuclear import factor. J. Cell Biol. 143, 309–318 (1998)
    Article CAS Google Scholar
  14. Harreman, M. T. et al. Characterization of the auto-inhibitory sequence within the N-terminal domain of importin α. J. Biol. Chem. 278, 21361–21369 (2003)
    Article CAS Google Scholar
  15. Gruss, O. J. et al. Ran induces spindle assembly by reversing the inhibitory effect of importin α on TPX2 activity. Cell 104, 83–93 (2001)
    Article CAS Google Scholar
  16. Behrens, P., Brinkmann, U. & Wellmann, A. CSE1L/CAS: Its role in proliferation and apoptosis. Apoptosis 8, 39–44 (2003)
    Article CAS Google Scholar
  17. Xiao, Z., McGrew, J. T., Schroeder, A. J. & Fitzgerald-Hayes, M. CSE1 and CSE2, two new genes required for accurate mitotic chromosome segregation in Saccharomyces cerevisiae. Mol. Cell. Biol. 13, 4691–4702 (1993)
    Article CAS Google Scholar
  18. Conti, E., Uy, M., Leighton, L., Blobel, G. & Kuriyan, J. Crystallographic analysis of the recognition of a nuclear localization signal by the nuclear import factor karyopherin α. Cell 94, 193–204 (1998)
    Article CAS Google Scholar
  19. Gorlich, D. et al. A novel class of RanGTP binding proteins. J. Cell Biol. 138, 65–80 (1997)
    Article CAS Google Scholar
  20. Vetter, I. R., Nowak, C., Nishimoto, T., Kuhlmann, J. & Wittinghofer, A. Structure of a Ran-binding domain complexed with Ran bound to a GTP analogue: implications for nuclear transport. Nature 398, 39–46 (1999)
    Article ADS CAS Google Scholar
  21. Collaborative Computational Project No. 4. The CCP4 suite: programs for protein crystallography. Acta Crystallogr. D 50, 760–763 (1994)
    Article Google Scholar
  22. Brunger, A. T. et al. Crystallography and NMR system: a new software suite for macromolecular structure determination. Acta Crystallogr. D 50, 905–921 (1998)
    Article Google Scholar
  23. Conti, E. & Kuriyan, J. Crystallographic analysis of the specific yet versatile recognition of distinct nuclear localization signals by karyopherin α. Struct. Fold. Des. 8, 329–338 (2000)
    Article CAS Google Scholar
  24. Perrakis, A., Morris, R. & Lamzin, V. S. Automated protein model building combined with iterative structure refinement. Nature Struct. Biol. 6, 458–463 (1999)
    Article CAS Google Scholar
  25. Jones, T. A., Cowan, S. & Kjelgaard, M. Improved methods for building protein models in electron density maps and the location of errors in these models. Acta Crystallogr. A 47, 110–119 (1991)
    Article Google Scholar
  26. Kraulis, P. J. MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures. J. Appl. Crystallogr. 24, 946–950 (1991)
    Article Google Scholar
  27. Merritt, E. A. & Bacon, D. J. Raster3D: photorealistic molecular graphics. Methods Enzymol. 277, 505–524 (1997)
    Article CAS Google Scholar
  28. Matsuura, Y., Lange, A., Harreman, M. T., Corbett, A. H. & Stewart, M. Structural basis for Nup2p function in cargo release and karyopherin recycling in nuclear import. EMBO J. 22, 5358–5369 (2003)
    Article CAS Google Scholar
  29. Askjaer, P. et al. RanGTP-regulated interactions of CRM1 with nucleoporins and a shuttling DEAD-box helicase. Mol. Cell. Biol. 19, 6276–6285 (1999)
    Article CAS Google Scholar

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