Somatic coding mutations in human induced pluripotent stem cells (original) (raw)

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Sequencing results for the mutations reported here are included in Supplementary Figure 1. Raw Illumina sequencing reads are available from the NCBI ShortRead Archive, accession SRP005709, except for lines derived from Hib11, Hib17, Hib29, CF, HFFxF, dH1F fibroblasts as the original donors were not consulted about public release of their genome data.

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Acknowledgements

We thank J. M. Akey, G. M. Church, S. Ding, J. B. Li and J. Shendure for discussions and suggestions, S. Vassallo for assistance with DNA shearing, and G. L. Boulting and S. Ratansirintrawoot for assistance with hiPS cell culture. This study is supported by NIH R01 HL094963 and a UCSD new faculty start-up fund (to K.Z.), a training grant from the California Institute for Regenerative Medicine (TG2-01154) and a CIRM grant (RC1-00116) (to L.S.B.G.). L.S.B.G. is an Investigator of the Howard Hughes Medical Institute. A. Gore is supported by the Focht-Powell Fellowship and a CIRM predoctoral fellowship. M.L.W. is supported by an institutional training grant from the National Institute of General Medical Sciences (T32 GM008666). Y.-H.L. is supported by the A*Star Institute of Medical Biology and the Singapore Stem Cell Consortium. Work in the laboratory of J.C.I.B. was supported by grants from MICINN, Sanofi-Aventis, the G. Harold and Leila Y. Mathers Foundation and the Cellex Foundation. G.Q.D. is an investigator of the Howard Hughes Medical Institute and supported by grants from the NIH.

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1. Athurva Gore and Zhe Li: These authors contributed equally to this work.

Authors and Affiliations

1. Department of Bioengineering, Institute for Genomic Medicine and Institute of Engineering in Medicine, University of California at San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA,
   Athurva Gore, Zhe Li, Ho-Lim Fung & Kun Zhang
2. Department of Cellular and Molecular Medicine and Howard Hughes Medical Institute, University of California at San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA,
   Jessica E. Young, Isabel Canto, Mason A. Israel, Melissa L. Wilbert & Lawrence S. B. Goldstein
3. Division of Pediatric Hematology/Oncology, Children’s Hospital Boston and Dana Farber Cancer Institute, Boston, 02115, Massachusetts, USA
   Suneet Agarwal, Yuin-Han Loh, Philip D. Manos & George Q. Daley
4. Department of Anatomy, University of Wisconsin-Madison, Madison, 53705, Wisconsin, USA
   Jessica Antosiewicz-Bourget, Junying Yu & James A. Thomson
5. Center of Regenerative Medicine, 08003 Barcelona, Spain,
   Alessandra Giorgetti, Nuria Montserrat & Juan Carlos Izpisua Belmonte
6. Department of Stem Cell and Regenerative Biology, Howard Hughes Medical Institute, Harvard Stem Cell Institute, Harvard University, Cambridge, 02138, Massachusetts, USA
   Evangelos Kiskinis & Kevin Eggan
7. Department of Genetics, Harvard Medical School, Boston, 02135, Massachusetts, USA
   Je-Hyuk Lee
8. Salk Institute for Biological Studies, La Jolla, 92037, California, USA
   Athanasia D. Panopoulos, Sergio Ruiz & Juan Carlos Izpisua Belmonte
9. The J. Craig Venter Institute, Rockville, 20850, Maryland, USA
   Ewen F. Kirkness
10. Immune Disease Institute, Children’s Hospital Boston, Boston, 02115, Massachusetts, USA
    Derrick J. Rossi

Authors

1. Athurva Gore
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2. Zhe Li
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3. Ho-Lim Fung
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4. Jessica E. Young
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5. Suneet Agarwal
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6. Jessica Antosiewicz-Bourget
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7. Isabel Canto
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8. Alessandra Giorgetti
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9. Mason A. Israel
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10. Evangelos Kiskinis
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11. Je-Hyuk Lee
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12. Yuin-Han Loh
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13. Philip D. Manos
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14. Nuria Montserrat
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15. Athanasia D. Panopoulos
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16. Sergio Ruiz
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17. Melissa L. Wilbert
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18. Junying Yu
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19. Ewen F. Kirkness
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20. Juan Carlos Izpisua Belmonte
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21. Derrick J. Rossi
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22. James A. Thomson
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23. Kevin Eggan
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24. George Q. Daley
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25. Lawrence S. B. Goldstein
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26. Kun Zhang
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Contributions

L.S.B.G. and K.Z. co-directed the study. A. Gore, Z.L., L.S.B.G. and K.Z. designed the experiments. J.E.Y., S.A., J.A.-B., I.C., A. Giorgetti, M.A.I., E.K., J.-H.L., Y.-H.L., P.D.M., N.M., A.D.P., S.R., M.L.W., J. Yu, J.C.I.B., D.J.R., J.A.T., K.E., G.Q.D. and L.S.B.G. biopsied, cultured and derived hiPS cell lines. Z.L. performed DNA extraction. A. Gore, Z.L. and K.Z. performed exome library construction, DigiQ library construction and validation Sanger sequencing. H.-L.F. performed Illumina sequencing. A. Gore and K.Z. performed bioinformatic and statistical analysis with contributions from E.F.K. A. Gore, Z.L., L.S.B.G., G.Q.D. and K.Z. wrote the manuscript with contributions from all other authors.

Corresponding authors

Correspondence toLawrence S. B. Goldstein or Kun Zhang.

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The authors declare no competing financial interests.

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Gore, A., Li, Z., Fung, HL. et al. Somatic coding mutations in human induced pluripotent stem cells.Nature 471, 63–67 (2011). https://doi.org/10.1038/nature09805

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• Received: 29 April 2010
• Accepted: 12 January 2011
• Published: 02 March 2011
• Issue Date: 03 March 2011
• DOI: https://doi.org/10.1038/nature09805