Multiplex amplification of large sets of human exons (original) (raw)

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In the version of this article initially published online,the affiliation for Jay Shendure was listed as Department of Computer Science, Virginia Commonwealth University,601 West Main Street,Richmond,Virginia 23284,USA. The correct affiliation should be Department of Genome Sciences,University of Washington,1705 NE Pacific St.,Seattle,Washington 98195,USA. The error has been corrected for all versions of the article.

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Acknowledgements

This work was supported by a Center for Excellence in Genome Sciences grant from the National Human Genome Research Institute, and a SPARC grant from the Broad Institute of Massachusetts Institute of Technology and Harvard University. We are grateful to G. Buck, M. Davis, N. Sheth, C. Childress, Jr. and J. Noble (Center for High Performance Computing and Center for the Study of Biological Complexity, Virginia Commonwealth University) for setting up the Illumina Genome Analyzer analysis pipeline. We thank H. Ji, S. Fredriksson, A. Gnirke, E. Lander, D. Jaffe and C. Nusbaum for discussions.

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Author notes

  1. Kun Zhang, Fredrik Dahl & Jay Shendure
    Present address: Present addresses: Department of Bioengineering, University of California at San Diego, 9500 Gilman Dr., La Jolla, California 92093, USA (K.Z.), Complete Genomics Inc., 2071 Stierlin Court, Suite 100, Mountain View, California 94043, USA (F.D.), and Department of Genome Sciences, University of Washington, 1705 NE Pacific St., Seattle, Washington 98195, USA (J.S.).,
  2. Gregory J Porreca, Kun Zhang, George M Church and Jay Shendure: These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, 02115, Massachusetts, USA
    Gregory J Porreca, Kun Zhang, Jin Billy Li, Sara L Vassallo, George M Church & Jay Shendure
  2. Center for the Study of Biological Complexity, Virginia Commonwealth University, 1000 W. Cary St., Richmond, 23284, Virginia, USA
    Bin Xie, Derek Austin & Yuan Gao
  3. Genomics Solution Unit, Agilent Technologies Inc., 5301 Stevens Creek Blvd., Santa Clara, 95051, California, USA
    Emily M LeProust & Bill J Peck
  4. Codon Devices Inc., One Kendall Square, Building 300, Third Floor, Cambridge, 02139, Massachusetts, USA
    Christopher J Emig
  5. Stanford Genome Technology Center, Clark Center W300, 318 Campus Drive, Stanford, 94305, California, USA
    Fredrik Dahl
  6. Department of Computer Science, Virginia Commonwealth University, 601 West Main Street, Richmond, 23284, Virginia, USA
    Yuan Gao

Authors

  1. Gregory J Porreca
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  2. Kun Zhang
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  3. Jin Billy Li
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  4. Bin Xie
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  5. Derek Austin
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  6. Sara L Vassallo
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  7. Emily M LeProust
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  8. Bill J Peck
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  9. Christopher J Emig
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  10. Fredrik Dahl
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  11. Yuan Gao
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  12. George M Church
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  13. Jay Shendure
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Corresponding author

Correspondence toJay Shendure.

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Competing interests

E.M.L. and B.J.P. are employed by Agilent Technologies, Inc., and Agilent reagents are used in the research presented in this article.

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Porreca, G., Zhang, K., Li, J. et al. Multiplex amplification of large sets of human exons.Nat Methods 4, 931–936 (2007). https://doi.org/10.1038/nmeth1110

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