Dopamine neurons derived from human ES cells efficiently engraft in animal models of Parkinson’s disease (original) (raw)

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Gene Expression Omnibus

Data deposits

The raw gene expression data generated in this study have been deposited in the Gene Expression Omnibus database under accession number GSE32658.

Change history

In the online-only Methods, in the "Neural induction" subsection, the concentration of DAPT appeared incorrectly as "10 nM" in the AOP PDF version. This has been corrected.

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Acknowledgements

We thank K. Manova, M. Tomishima and A. Viale for excellent technical support, and R. McKay for the anti-nestin antibody. The work was supported by NIH/NINDS grant NS052671, the European Commission project NeuroStemcell, the Starr foundation and NYSTEM contract C024414 to L.S.; by NYSTEM contract C024413, the Michael T. McCarthy Foundation and the Elkus Family Foundation to V.T.; by the Consolidated Anti-Aging Foundation to J.H.K.; by NIH/NINDS grant P50 NS047085, and support from Falk Medical Research Trust to D.J.S.; J.-W.S. was supported by NYSCF (Druckenmiller fellowship) and S.K. by a Starr stem cell scholar fellowship.

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Author notes

  1. Sonja Kriks and Jae-Won Shim: These authors contributed equally to this work.

Authors and Affiliations

  1. Center for Stem Cell Biology, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, New York 10065, USA,
    Sonja Kriks, Jae-Won Shim, Jinghua Piao, Yosif M. Ganat, Gordon Auyeung, Chris Antonacci, Amanda Buch, Viviane Tabar & Lorenz Studer
  2. Developmental Biology Program, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, New York 10065, USA,
    Sonja Kriks, Jae-Won Shim, Yosif M. Ganat & Lorenz Studer
  3. Department of Neurosurgery, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, New York 10065, USA,
    Jinghua Piao, Gordon Auyeung, Chris Antonacci, Amanda Buch, Viviane Tabar & Lorenz Studer
  4. Department of Neurological Sciences, Rush University Medical Center, Chicago, 60612, Illinois, USA
    Dustin R. Wakeman & Jeffrey H. Kordower
  5. Department of Physiology, Feinberg School of Medicine, Northwestern University, Chicago, 60611, Illinois, USA
    Zhong Xie, Luis Carrillo-Reid & D. James Surmeier
  6. Department of Neurology and Neuroscience, Weill Medical College of Cornell University, New York Presbyterian Hospital, 525 East 68th Street, New York, New York 10021, USA,
    Lichuan Yang & M. Flint Beal

Authors

  1. Sonja Kriks
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  2. Jae-Won Shim
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  3. Jinghua Piao
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  4. Yosif M. Ganat
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  5. Dustin R. Wakeman
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  6. Zhong Xie
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  7. Luis Carrillo-Reid
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  8. Gordon Auyeung
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  9. Chris Antonacci
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  10. Amanda Buch
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  11. Lichuan Yang
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  12. M. Flint Beal
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  13. D. James Surmeier
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  14. Jeffrey H. Kordower
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  15. Viviane Tabar
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  16. Lorenz Studer
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Contributions

S.K. and J.-W.S.: conception and study design, maintenance and directed differentiation of PSCs, cellular/molecular assays, histological analyses, mouse behavioural assays, data interpretation and writing of manuscript. J.P., G.A., C.A. and A.B.: rat transplantation, histological analyses and behavioural assays. Y.M.G.: mouse transplantation and histological analyses. D.R.W. and J.H.K.: monkey transplantation, histological analysis and data interpretation. L.Y. and M.F.B.: HPLC analysis and data interpretation. L.C.-R., Z.X and D.J.S.: electrophysiological analyses and data interpretation. V.T.: study design, data analysis and writing of manuscript. L.S.: conception and study design, data analysis and interpretation, and writing of manuscript.

Corresponding author

Correspondence toLorenz Studer.

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The authors declare no competing financial interests.

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Kriks, S., Shim, JW., Piao, J. et al. Dopamine neurons derived from human ES cells efficiently engraft in animal models of Parkinson’s disease.Nature 480, 547–551 (2011). https://doi.org/10.1038/nature10648

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