NANOG-dependent function of TET1 and TET2 in establishment of pluripotency (original) (raw)

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Acknowledgements

We thank W. Mansfield for blastocyst injections, A. Radzisheuskaya for cell culture assistance, and R. Jaenisch for _Tet1_−/− embryonic stem cells. This study was supported by a grant from the NIH (1R01-GM095942-01A1), a grant from New York state Department of Health (NYSTEM#C026420), and a seed fund from the Black Family Stem Cell Institute to J.W., by the Wellcome Trust Fellowship (WT086692MA) and the Isaac Newton Trust Grant (11.19(ad)) to J.C.R.S., who is a Wellcome Trust Career Development Fellow, by the BBSRC, the MRC, the Wellcome Trust, and EU Epigenesys and BLUEPRINT to W.R., and by the Wellcome Trust Fellowship WT079249 to T.W.T.

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Author notes

  1. Yael Costa, Junjun Ding, Thorold W. Theunissen, Francesco Faiola, José C. R. Silva and Jianlong Wang: These authors contributed equally to this work.

Authors and Affiliations

  1. Wellcome Trust-Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK,
    Yael Costa, Thorold W. Theunissen, Moyra Lawrence, Sabine Dietmann & José C. R. Silva
  2. Department of Developmental and Regenerative Biology, Black Family Stem Cell Institute, Graduate School of Biological Sciences, Mount Sinai School of Medicine, New York, 10029, New York, USA
    Junjun Ding, Francesco Faiola, Miguel Fidalgo, Arven Saunders & Jianlong Wang
  3. Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK,
    Thorold W. Theunissen, Moyra Lawrence & José C. R. Silva
  4. Epigenetics Programme, The Babraham Institute, Cambridge CB22 3AT, UK,
    Timothy A. Hore & Wolf Reik
  5. Cambridge Centre for Proteomics, Cambridge Systems Biology Centre, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK,
    Pavel V. Shliaha
  6. Department of Internal Medicine, and Program in Molecular and Cellular Biology, University of Iowa, Iowa City, 52242, Iowa, USA
    Satyabrata Das & Dana N. Levasseur
  7. Department of Pediatrics, Indiana University, Indianapolis, 46202, Indiana, USA
    Zhe Li & Mingjiang Xu
  8. Centre for Trophoblast Research, University of Cambridge, Cambridge CB2 3EG, UK,
    Wolf Reik

Authors

  1. Yael Costa
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  2. Junjun Ding
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  3. Thorold W. Theunissen
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  4. Francesco Faiola
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  5. Timothy A. Hore
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  6. Pavel V. Shliaha
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  7. Miguel Fidalgo
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  8. Arven Saunders
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  9. Moyra Lawrence
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  10. Sabine Dietmann
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  11. Satyabrata Das
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  12. Dana N. Levasseur
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  13. Zhe Li
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  14. Mingjiang Xu
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  15. Wolf Reik
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  16. José C. R. Silva
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  17. Jianlong Wang
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Contributions

J.C.R.S. and J.W. conceived the project, designed the experiments, prepared and approved the manuscript. T.W.T. designed and performed experiments and wrote the manuscript draft. Y.C., J.D., F.F., M.F. and A.S. designed and performed experiments and prepared the manuscript. T.A.H. designed and performed experiments. P.V.S. performed interactomics data analysis. M.L. provided technical assistance. S.Di. provided bioinformatic analysis. S.Da., D.N.L., Z.L. and M.X. contributed to the reagents. W.R. designed experiments and contributed to the reagents. J.C.R.S. and J.W. are equal senior and corresponding authors.

Corresponding authors

Correspondence toJosé C. R. Silva or Jianlong Wang.

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The authors declare no competing financial interests.

Supplementary information

Supplementary Information

This file contains Supplementary Methods, Supplementary Figures 1-16, Supplementary Tables 1-3 and 5-6 (see separate excel file for Supplementary Table 4), and Supplementary References. (PDF 3821 kb)

Supplementary Table 4

This file contains the genomic coordinates of NANOG and TET1 co-bound sites. (XLS 743 kb)

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Costa, Y., Ding, J., Theunissen, T. et al. NANOG-dependent function of TET1 and TET2 in establishment of pluripotency.Nature 495, 370–374 (2013). https://doi.org/10.1038/nature11925

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