Transgenic mice with defined combinations of drug-inducible reprogramming factors (original) (raw)

Nature Biotechnology volume 27, pages 169–171 (2009)Cite this article

Abstract

Proviruses carrying drug-inducible Oct4, Sox2, Klf4 and c-Myc used to derive 'primary' induced pluripotent stem (iPS) cells were segregated through germline transmission, generating mice and cells carrying subsets of the reprogramming factors. Drug treatment produced 'secondary' iPS cells only when the missing factor was introduced. This approach creates a defined system for studying reprogramming mechanisms and allows screening of genetically homogeneous cells for compounds that can replace any transcription factor required for iPS cell derivation.

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Acknowledgements

We thank G. Bell, R. Flannery and members of the Jaenisch lab for assistance and comments. R.J. is supported by grants from the National Institutes of Health: 5-RO1-HDO45022, 5-R37-CA084198 and 5-RO1-CA087869. J.H. is a Novartis Fellow by the Helen Hay Whitney Foundation.

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Author notes

  1. Styliani Markoulaki and Jacob Hanna: These authors contributed equally to this work.

Authors and Affiliations

  1. The Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, 02142, Massachusetts, USA
    Styliani Markoulaki, Jacob Hanna, Caroline Beard, Bryce W Carey, Albert W Cheng, Christopher J Lengner, Jessica A Dausman, Dongdong Fu, Qing Gao, Su Wu, John P Cassady & Rudolf Jaenisch
  2. Department of Biology, Massachusetts Institute of Technology, 31 Ames Street, Cambridge, 02142, Massachusetts, USA
    Bryce W Carey, Albert W Cheng, John P Cassady & Rudolf Jaenisch

Authors

  1. Styliani Markoulaki
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  2. Jacob Hanna
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  3. Caroline Beard
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  4. Bryce W Carey
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  5. Albert W Cheng
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  6. Christopher J Lengner
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  7. Jessica A Dausman
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  8. Dongdong Fu
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  9. Qing Gao
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  10. Su Wu
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  11. John P Cassady
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  12. Rudolf Jaenisch
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Corresponding author

Correspondence toRudolf Jaenisch.

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Competing interests

R.J. is an advisor to Stemgent.

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Markoulaki, S., Hanna, J., Beard, C. et al. Transgenic mice with defined combinations of drug-inducible reprogramming factors.Nat Biotechnol 27, 169–171 (2009). https://doi.org/10.1038/nbt.1520

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