Transgenic mice with defined combinations of drug-inducible reprogramming factors (original) (raw)
- Brief Communication
- Published: 18 January 2009
- Jacob Hanna1 na1,
- Caroline Beard1,
- Bryce W Carey1,2,
- Albert W Cheng1,2,
- Christopher J Lengner1,
- Jessica A Dausman1,
- Dongdong Fu1,
- Qing Gao1,
- Su Wu1,
- John P Cassady1,2 &
- …
- Rudolf Jaenisch1,2
Nature Biotechnology volume 27, pages 169–171 (2009)Cite this article
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Abstract
Proviruses carrying drug-inducible Oct4, Sox2, Klf4 and c-Myc used to derive 'primary' induced pluripotent stem (iPS) cells were segregated through germline transmission, generating mice and cells carrying subsets of the reprogramming factors. Drug treatment produced 'secondary' iPS cells only when the missing factor was introduced. This approach creates a defined system for studying reprogramming mechanisms and allows screening of genetically homogeneous cells for compounds that can replace any transcription factor required for iPS cell derivation.
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References
- Jaenisch, R. & Young, R. Cell 132, 567–582 (2008).
Article CAS PubMed PubMed Central Google Scholar - Takahashi, K. & Yamanaka, S. Cell 126, 663–676 (2006).
CAS PubMed Google Scholar - Wernig, M. et al. Nature 448, 318–324 (2007).
Article CAS PubMed Google Scholar - Takahashi, K. et al. Cell 131, 861–872 (2007).
Article CAS PubMed Google Scholar - Hanna, J. et al. Science 318, 1920–1923 (2007).
Article CAS PubMed Google Scholar - Shi, Y. et al. Cell Stem Cell 3, 568–574 (2008).
Article CAS PubMed Google Scholar - Marson, A. et al. Cell Stem Cell 3, 132–135 (2008).
Article CAS PubMed PubMed Central Google Scholar - Huangfu, D. et al. Nat. Biotechnol. 26, 1269–1275 (2008).
Article CAS PubMed Google Scholar - Wernig, M. et al. Nat. Biotechnol. 26, 916–924 (2008).
Article CAS PubMed PubMed Central Google Scholar - Hanna, J. et al. Cell 133, 250–264 (2008).
Article CAS PubMed PubMed Central Google Scholar - Wernig, M. et al. Cell Stem Cell 2, 10–12 (2008).
Article CAS PubMed Google Scholar - Nakagawa, M. et al. Nat. Biotechnol. 26, 101–106 (2008).
Article CAS PubMed Google Scholar
Acknowledgements
We thank G. Bell, R. Flannery and members of the Jaenisch lab for assistance and comments. R.J. is supported by grants from the National Institutes of Health: 5-RO1-HDO45022, 5-R37-CA084198 and 5-RO1-CA087869. J.H. is a Novartis Fellow by the Helen Hay Whitney Foundation.
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- Styliani Markoulaki and Jacob Hanna: These authors contributed equally to this work.
Authors and Affiliations
- The Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, 02142, Massachusetts, USA
Styliani Markoulaki, Jacob Hanna, Caroline Beard, Bryce W Carey, Albert W Cheng, Christopher J Lengner, Jessica A Dausman, Dongdong Fu, Qing Gao, Su Wu, John P Cassady & Rudolf Jaenisch - Department of Biology, Massachusetts Institute of Technology, 31 Ames Street, Cambridge, 02142, Massachusetts, USA
Bryce W Carey, Albert W Cheng, John P Cassady & Rudolf Jaenisch
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- Styliani Markoulaki
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Correspondence toRudolf Jaenisch.
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R.J. is an advisor to Stemgent.
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Markoulaki, S., Hanna, J., Beard, C. et al. Transgenic mice with defined combinations of drug-inducible reprogramming factors.Nat Biotechnol 27, 169–171 (2009). https://doi.org/10.1038/nbt.1520
- Received: 11 September 2008
- Accepted: 19 December 2008
- Published: 18 January 2009
- Issue Date: February 2009
- DOI: https://doi.org/10.1038/nbt.1520