Genetic engineering of human pluripotent cells using TALE nucleases (original) (raw)

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Acknowledgements

We thank R. Alagappan, P. Xu, S. Cristea, S. Lam and A. Vincent for expert technical assistance. We thank F. Soldner for helpful discussions on the manuscript. D.H. is a Merck Fellow of the Life Sciences Research Foundation. R.J. was supported by US National Institutes of Health grants R37-CA084198, RO1-CA087869 and RO1-HD045022 and by a grant from the Howard Hughes Medical Institute.

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Author notes

  1. Dirk Hockemeyer and Haoyi Wang: These authors contributed equally to this work.

Authors and Affiliations

  1. The Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA
    Dirk Hockemeyer, Haoyi Wang, Samira Kiani, Christine S Lai, Qing Gao, John P Cassady & Rudolf Jaenisch
  2. Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    Christine S Lai, John P Cassady & Rudolf Jaenisch
  3. Sangamo BioSciences, Inc., Richmond, California, USA
    Gregory J Cost, Lei Zhang, Yolanda Santiago, Jeffrey C Miller, Bryan Zeitler, Jennifer M Cherone, Xiangdong Meng, Sarah J Hinkley, Edward J Rebar, Philip D Gregory & Fyodor D Urnov

Authors

  1. Dirk Hockemeyer
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  2. Haoyi Wang
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  3. Samira Kiani
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  4. Christine S Lai
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  5. Qing Gao
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  6. John P Cassady
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  7. Gregory J Cost
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  8. Lei Zhang
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  9. Yolanda Santiago
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  10. Jeffrey C Miller
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  11. Bryan Zeitler
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  12. Jennifer M Cherone
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  13. Xiangdong Meng
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  14. Sarah J Hinkley
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  15. Edward J Rebar
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  16. Philip D Gregory
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  17. Fyodor D Urnov
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  18. Rudolf Jaenisch
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Contributions

D.H., H.W. and R.J. designed the targeting experiments and wrote the manuscript. H.W. and D.H. generated donor plasmids. D.H. preformed targeting experiments. S.K., C.S.L. and H.W. assisted with Southern blot analysis. Q.G. analyzed teratomas. J.P.C. and D.H. performed FACS analysis of targeted cells. L.Z. and J.C.M. designed the TALENs, S.J.H. assembled the TALENs, G.J.C. and Y.S. tested the TALENs, and B.Z., J.M.C. and X.M. performed the off-target analysis. D.H., R.J., L.Z., G.J.C., J.C.M., B.Z., X.M. and F.D.U. analyzed the data. E.J.R., P.D.G. and F.D.U. designed and supervised the design of the TALENs and contributed to writing the manuscript.

Corresponding author

Correspondence toRudolf Jaenisch.

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Competing interests

R.J. is an adviser to Stemgent and a cofounder of Fate Therapeutics. G.J.C., L.Z., Y.S., J.C.M., B.Z., J.M.C., X.M., S.J.H., E.J.R., P.D.G. and F.D.U. are full-time employees of Sangamo BioSciences.

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Hockemeyer, D., Wang, H., Kiani, S. et al. Genetic engineering of human pluripotent cells using TALE nucleases.Nat Biotechnol 29, 731–734 (2011). https://doi.org/10.1038/nbt.1927

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