Genetic engineering of human pluripotent cells using TALE nucleases (original) (raw)
This is a preview of subscription content, access via your institution
References
- Zou, J. et al. Cell Stem Cell 5, 97–110 (2009).
Article PubMed PubMed Central Google Scholar - Hockemeyer, D. et al. Nat. Biotechnol. 27, 851–857 (2009).
Article PubMed PubMed Central Google Scholar - Hockemeyer, D. & Jaenisch, R. Cold Spring Harb. Symp. Quant. Biol. 75, 201–209 (2010).
Article PubMed Google Scholar - Urnov, F.D., Rebar, E.J., Holmes, M.C., Zhang, H.S. & Gregory, P.D. Nat. Rev. Genet. 11, 636–646 (2010).
Article PubMed Google Scholar - Soldner, F. et al. Cell (in the press).
- Boch, J. & Bonas, U. Annu. Rev. Phytopathol. 48, 419–436 (2010).
Article PubMed Google Scholar - Boch, J. et al. Science 326, 1509–1512 (2009).
Article PubMed Google Scholar - Moscou, M.J. & Bogdanove, A.J. Science 326, 1501 (2009).
Article PubMed Google Scholar - Miller, J.C. et al. Nat. Biotechnol. 29, 143–148 (2011).
Article PubMed Google Scholar - Cermak, T. et al. published online, doi:10.1093/nar/gkr218 (14 April 2011).
- Lengner, C.J. et al. Cell 141, 872–883 (2010).
Article PubMed Google Scholar - Hockemeyer, D. et al. Cell Stem Cell 3, 346–353 (2008).
Article PubMed PubMed Central Google Scholar - Zwaka, T. & Thomson, J. Nat. Biotechnol. 21, 319–321 (2003).
Article PubMed Google Scholar - Doyon, Y. et al. Nat. Methods 8, 74–79 (2011).
Article PubMed Google Scholar - Zhang, F. et al. Nat. Biotechnol. 29, 149–153 (2011).
Article PubMed PubMed Central Google Scholar
Acknowledgements
We thank R. Alagappan, P. Xu, S. Cristea, S. Lam and A. Vincent for expert technical assistance. We thank F. Soldner for helpful discussions on the manuscript. D.H. is a Merck Fellow of the Life Sciences Research Foundation. R.J. was supported by US National Institutes of Health grants R37-CA084198, RO1-CA087869 and RO1-HD045022 and by a grant from the Howard Hughes Medical Institute.
Author information
Author notes
- Dirk Hockemeyer and Haoyi Wang: These authors contributed equally to this work.
Authors and Affiliations
- The Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA
Dirk Hockemeyer, Haoyi Wang, Samira Kiani, Christine S Lai, Qing Gao, John P Cassady & Rudolf Jaenisch - Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
Christine S Lai, John P Cassady & Rudolf Jaenisch - Sangamo BioSciences, Inc., Richmond, California, USA
Gregory J Cost, Lei Zhang, Yolanda Santiago, Jeffrey C Miller, Bryan Zeitler, Jennifer M Cherone, Xiangdong Meng, Sarah J Hinkley, Edward J Rebar, Philip D Gregory & Fyodor D Urnov
Authors
- Dirk Hockemeyer
You can also search for this author inPubMed Google Scholar - Haoyi Wang
You can also search for this author inPubMed Google Scholar - Samira Kiani
You can also search for this author inPubMed Google Scholar - Christine S Lai
You can also search for this author inPubMed Google Scholar - Qing Gao
You can also search for this author inPubMed Google Scholar - John P Cassady
You can also search for this author inPubMed Google Scholar - Gregory J Cost
You can also search for this author inPubMed Google Scholar - Lei Zhang
You can also search for this author inPubMed Google Scholar - Yolanda Santiago
You can also search for this author inPubMed Google Scholar - Jeffrey C Miller
You can also search for this author inPubMed Google Scholar - Bryan Zeitler
You can also search for this author inPubMed Google Scholar - Jennifer M Cherone
You can also search for this author inPubMed Google Scholar - Xiangdong Meng
You can also search for this author inPubMed Google Scholar - Sarah J Hinkley
You can also search for this author inPubMed Google Scholar - Edward J Rebar
You can also search for this author inPubMed Google Scholar - Philip D Gregory
You can also search for this author inPubMed Google Scholar - Fyodor D Urnov
You can also search for this author inPubMed Google Scholar - Rudolf Jaenisch
You can also search for this author inPubMed Google Scholar
Contributions
D.H., H.W. and R.J. designed the targeting experiments and wrote the manuscript. H.W. and D.H. generated donor plasmids. D.H. preformed targeting experiments. S.K., C.S.L. and H.W. assisted with Southern blot analysis. Q.G. analyzed teratomas. J.P.C. and D.H. performed FACS analysis of targeted cells. L.Z. and J.C.M. designed the TALENs, S.J.H. assembled the TALENs, G.J.C. and Y.S. tested the TALENs, and B.Z., J.M.C. and X.M. performed the off-target analysis. D.H., R.J., L.Z., G.J.C., J.C.M., B.Z., X.M. and F.D.U. analyzed the data. E.J.R., P.D.G. and F.D.U. designed and supervised the design of the TALENs and contributed to writing the manuscript.
Corresponding author
Correspondence toRudolf Jaenisch.
Ethics declarations
Competing interests
R.J. is an adviser to Stemgent and a cofounder of Fate Therapeutics. G.J.C., L.Z., Y.S., J.C.M., B.Z., J.M.C., X.M., S.J.H., E.J.R., P.D.G. and F.D.U. are full-time employees of Sangamo BioSciences.
Supplementary information
Rights and permissions
About this article
Cite this article
Hockemeyer, D., Wang, H., Kiani, S. et al. Genetic engineering of human pluripotent cells using TALE nucleases.Nat Biotechnol 29, 731–734 (2011). https://doi.org/10.1038/nbt.1927
- Received: 11 March 2011
- Accepted: 28 June 2011
- Published: 01 August 2011
- Issue Date: August 2011
- DOI: https://doi.org/10.1038/nbt.1927